I2D I2D uberti-2010-1 Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. human-pbmc Human Peripheral Blood Mononuclear cell peripheral_blood_mononuclear_cell Peripheral blood mononuclear cell anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database uberti-2010-5 Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. mouse-h_end Mus musculus immortalized heart endothelial cells h_end H-End Murine endothelioma The h.end cell line was established from heart cells taken from a DBAI2xC57BV6 mouse fetus at day 13 gestation and immortalized by infection with the retrovirus vector N-TKmT containing PmT. This cell line was characterized as EC on the basis of expression od CD31, factor VIII, uptake of acetylated low-density lipoproteins and synthesis of platelet-activating factor and chemokines after stimulation with IL-1 or TNF. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database uberti-2010-2 Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database uberti-2010-3 Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Extracts of endothelial progenitor cells isolated from peripheral blood mononuclear cells were used as the source of intergrin beta 1. Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database uberti-2010-4 Inhibition of beta1 integrin and IL-3Rbeta common subunit interaction hinders tumour angiogenesis. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Uberti B., Dentelli P., Rosso A., Defilippi P., Brizzi MF. Cancer - Interactions investigated in the context of cancer Extracts of endothelial cells isolated and cultured from human umbilical veins within 4hr of delivery were used as the source of intergrin beta 1. Endothelial progenitor cells were isolated from peripheral blood mononuclear cells. mariafelice.brizzi@unito.it Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database il3rb_human Cytokine receptor common subunit beta CSF2RB IL3RB IL5RB GM-CSF/IL-3/IL-5 receptor common beta subunit CDw131 protein protein human Homo sapiens Human MVLAQGLLSMALLALCWERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMWVLALIVIFLTIAVLLALRFCGIYGYRLRRKWEEKIPNPSKSHLFQNGSAELWPPGSMSAFTSGSPPHQGPWGSRFPELEGVFPVGFGDSEVSPLTIEDPKHVCDPPSGPDTTPAASDLPTEQPPSPQPGPPAASHTPEKQASSFDFNGPYLGPPHSRSLPDILGQPEPPQEGGSQKSPPPGSLEYLCLPAGGQVQLVPLAQAMGPGQAVEVERRPSQGAAGSPSLESGGGPAPPALGPRVGGQDQKDSPVAIPMSSGDTEDPGVASGYVSSADLVFTPNSGASSVSLVPSLGLPSDQTPSLCPGLASGPPGAPGPVKSGFEGYVELPPIEGRSPRSPRNNPVPPEAKSPVLNPGERPADVSPTSPQPEGLLVLQQVGDYCFLPGLGPGPLSLRSKPSSPGPGPEIKNLDQAFQVKKPPGQAVPQVPVIQLFKALKQQDYLSLPPWEVNKPGEVC itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK il3rb_mouse Cytokine receptor common subunit beta Csf2rb Aic2b Csf2rb1 Il3rb1 GM-CSF/IL-3/IL-5 receptor common beta subunit protein protein mouse Mus musculus Mouse MDQQMALTWGLCYMALVALCWGHGVTEAEETVPLKTLQCYNDYTNHIICSWADTEDAQGLINMTLYHQLEKKQPVSCELSEELMWSECPSSHRCVPRRCVIPYTRFSITNEDYYSFRPDSDLGIQLMVPLAQNVQPPLPKNVSISSSEDRFLLEWSVSLGDAQVSWLSSKDIEFEVAYKRLQDSWEDAYSLHTSKFQVNFEPKLFLPNSIYAARVRTRLSPGSSLSGRPSRWSPEVHWDSQPGDKAQPQNLQCFFDGIQSLHCSWEVWTQTTGSVSFGLFYRPSPVAPEEKCSPVVKEPPGASVYTRYHCSLPVPEPSAHSQYTVSVKHLEQGKFIMSYNHIQMEPPTLNLTKNRDSYSLHWETQKMAYSFIEHTFQVQYKKKSDSWEDSKTENLDRAHSMDLSQLEPDTSYCARVRVKPISNYDGIWSKWSEEYTWKTDWVMPTLWIVLILVFLILTLLLILRFGCVSVYRTYRKWKEKIPNPSKSLLFQDGGKGLWPPGSMAAFATKNPALQGPQSRLLAEQQGESYAHLEDNNVSPLTIEDPNIIRVPPSGPDTTPAASSESTEQLPNVQVEGPTPNRPRKQLPSFDFNGPYLGPPQSHSLPDLPDQLGSPQVGGSLKPALPGSLEYMCLPPGGQAQLVPLSQVMGQGQAMDVQCGSSLETSGSPSVEPKENPPVELSMEEQEARDNPVTLPISSGGPEGSMMASDYVTPGDPVLTLPTGPLSTSLGPSLGLPSAQSPSLCLKLPRVPSGSPALGPPGFEDYVELPPSVSQAAKSPPGHPAPPVASSPTVIPGEPREEVGPASPHPEGLLVLQQVGDYCFLPGLGPGSLSPHSKPPSPSLCSETEDLVQDLSVKKFPYQPMPQAPAIQFFKSLKHQDYLSLPPWDNSQSGKVC itb1_mouse Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta Itgb1 protein protein mouse Mus musculus Mouse MNLQLVSWIGLISLICSVFGQTDKNRCLKANAKSCGECIQAGPNCGWCTNTTFLQEGMPTSARCDDLEALKKKGCQPSDIENPRGSQTIKKNKNVTNRSKGMAEKLRPEDITQIQPQQLLLKLRSGEPQKFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTDRGEFFNELVGQQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNVYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSGNSSNVIQLIIDAYNSLSSEVILENSKLPDGVTINYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITANKCPNKESETIKIKPLGFTEEVEVVLQFICKCNCQSHGIPASPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCRCRVCECYPNYTGSACDCSLDTGPCLASNGQICNGRGICECGACKCTDPKFQGPTCETCQTCLGVCAEHKECVQCRAFNKGEKKDTCAQECSHFNLTKVESREKLPQPVQVDPVTHCKEKDIDDCWFYFTYSVNGNNEAIVHVVETPDCPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK il-3rbc-integrinb1 1008905 n/a 1008910 unspecified role unspecified role bait bait n/a 1008911 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1a, 4a il-3rbc-integrinb1-1 1008905 n/a 1008910 unspecified role unspecified role prey prey n/a 1008911 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1a, 4a il-3rbc-integrinb1-5 1008906 n/a 1008912 unspecified role unspecified role prey prey n/a 1008913 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4a il-3rbc-integrinb1-6 1008906 n/a 1008913 unspecified role unspecified role prey prey n/a 1008912 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4a il-3rbc-integrinb1-2 1008907 n/a 1008911 unspecified role unspecified role prey prey n/a 1008910 unspecified role unspecified role bait bait physical association physical association false false false Fig. S2b il-3rbc-integrinb1-3 1008908 n/a 1008910 unspecified role unspecified role bait bait chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 1008911 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-pbmc Human Peripheral Blood Mononuclear cell peripheral_blood_mononuclear_cell Peripheral blood mononuclear cell direct interaction direct interaction false false false Fig. 2b il-3rbc-integrinb1-4 1008909 n/a 1008910 unspecified role unspecified role bait bait chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 n/a 1008911 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-pbmc Human Peripheral Blood Mononuclear cell peripheral_blood_mononuclear_cell Peripheral blood mononuclear cell direct interaction direct interaction false false false Fig. S3a