IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ muntean-2010-2 The PAF complex synergizes with MLL fusion proteins at HOX loci to promote leukemogenesis. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Muntean AG., Tan J., Sitwala K., Huang Y., Bronstein J., Connelly JA., Basrur V., Elenitoba-Johnson KS., Hess JL. Apoptosis - Interactions involving proteins with a function related to apoptosis Species of constructs supplied by authors. jayhess@umich.edu Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer muntean-2010-7 The PAF complex synergizes with MLL fusion proteins at HOX loci to promote leukemogenesis. mouse-bone-marrow Mouse bone marrow bone_marrow bone marrow ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Muntean AG., Tan J., Sitwala K., Huang Y., Bronstein J., Connelly JA., Basrur V., Elenitoba-Johnson KS., Hess JL. Apoptosis - Interactions involving proteins with a function related to apoptosis Cell lines were generated by transducing mouse bone marrow cells with either (human) MLL-ENL or E2A-HLF. Species of constructs supplied by authors. jayhess@umich.edu Further interactions involving histone H3 species cannot be entered due to the lack of specificity of the antibodies used. Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer muntean-2010-4 The PAF complex synergizes with MLL fusion proteins at HOX loci to promote leukemogenesis. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Muntean AG., Tan J., Sitwala K., Huang Y., Bronstein J., Connelly JA., Basrur V., Elenitoba-Johnson KS., Hess JL. Apoptosis - Interactions involving proteins with a function related to apoptosis Species of constructs supplied by authors. jayhess@umich.edu Further interactions involving histone H3 species cannot be entered due to the lack of specificity of the antibodies used. Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer muntean-2010-3 The PAF complex synergizes with MLL fusion proteins at HOX loci to promote leukemogenesis. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Muntean AG., Tan J., Sitwala K., Huang Y., Bronstein J., Connelly JA., Basrur V., Elenitoba-Johnson KS., Hess JL. Apoptosis - Interactions involving proteins with a function related to apoptosis Species of constructs supplied by authors. jayhess@umich.edu Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer muntean-2010-6 The PAF complex synergizes with MLL fusion proteins at HOX loci to promote leukemogenesis. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Muntean AG., Tan J., Sitwala K., Huang Y., Bronstein J., Connelly JA., Basrur V., Elenitoba-Johnson KS., Hess JL. Apoptosis - Interactions involving proteins with a function related to apoptosis Species of constructs supplied by authors. jayhess@umich.edu Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer muntean-2010-1 The PAF complex synergizes with MLL fusion proteins at HOX loci to promote leukemogenesis. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Muntean AG., Tan J., Sitwala K., Huang Y., Bronstein J., Connelly JA., Basrur V., Elenitoba-Johnson KS., Hess JL. Apoptosis - Interactions involving proteins with a function related to apoptosis Species of constructs supplied by authors. jayhess@umich.edu Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer muntean-2010-5 The PAF complex synergizes with MLL fusion proteins at HOX loci to promote leukemogenesis. human-kopn_8 Human KOPN8 B cell precursor leukemia line (MLL-ENL fusion gene. kopn_8 Human B cell precursor leukemia cell line (MLL-ENL fusion) Established from the peripheral blood of a 3-month-old girl with B cell precursor acute lymphoblastic leukemia (BCP-ALL) of type B-III in 1977; cells were described to carry the t(11;19)(q23;p13) leading to the MLL-ENL fusion gene. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Muntean AG., Tan J., Sitwala K., Huang Y., Bronstein J., Connelly JA., Basrur V., Elenitoba-Johnson KS., Hess JL. Apoptosis - Interactions involving proteins with a function related to apoptosis Species of constructs supplied by authors. jayhess@umich.edu Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer kmt2a_human Histone-lysine N-methyltransferase 2A KMT2A ALL-1 ALL1 CXXC-type zinc finger protein 7 CXXC7 HRX HTRX MLL MLL1 Myeloid/lymphoid or mixed-lineage leukemia Myeloid/lymphoid or mixed-lineage leukemia protein 1 TRX1 Trithorax-like protein Zinc finger protein HRX Cysteine methyltransferase KMT2A protein protein human Homo sapiens Human MAHSCRWRFPARPGTTGGGGGGGRRGLGGAPRQRVPALLLPPGPPVGGGGPGAPPSPPAVAAAAAAAGSSGAGVPGGAAAASAASSSSASSSSSSSSSASSGPALLRVGPGFDAALQVSAAIGTNLRRFRAVFGESGGGGGSGEDEQFLGFGSDEEVRVRSPTRSPSVKTSPRKPRGRPRSGSDRNSAILSDPSVFSPLNKSETKSGDKIKKKDSKSIEKKRGRPPTFPGVKIKITHGKDISELPKGNKEDSLKKIKRTPSATFQQATKIKKLRAGKLSPLKSKFKTGKLQIGRKGVQIVRRRGRPPSTERIKTPSGLLINSELEKPQKVRKDKEGTPPLTKEDKTVVRQSPRRIKPVRIIPSSKRTDATIAKQLLQRAKKGAQKKIEKEAAQLQGRKVKTQVKNIRQFIMPVVSAISSRIIKTPRRFIEDEDYDPPIKIARLESTPNSRFSAPSCGSSEKSSAASQHSSQMSSDSSRSSSPSVDTSTDSQASEEIQVLPEERSDTPEVHPPLPISQSPENESNDRRSRRYSVSERSFGSRTTKKLSTLQSAPQQQTSSSPPPPLLTPPPPLQPASSISDHTPWLMPPTIPLASPFLPASTAPMQGKRKSILREPTFRWTSLKHSRSEPQYFSSAKYAKEGLIRKPIFDNFRPPPLTPEDVGFASGFSASGTAASARLFSPLHSGTRFDMHKRSPLLRAPRFTPSEAHSRIFESVTLPSNRTSAGTSSSGVSNRKRKRKVFSPIRSEPRSPSHSMRTRSGRLSSSELSPLTPPSSVSSSLSISVSPLATSALNPTFTFPSHSLTQSGESAEKNQRPRKQTSAPAEPFSSSSPTPLFPWFTPGSQTERGRNKDKAPEELSKDRDADKSVEKDKSRERDREREKENKRESRKEKRKKGSEIQSSSALYPVGRVSKEKVVGEDVATSSSAKKATGRKKSSSHDSGTDITSVTLGDTTAVKTKILIKKGRGNLEKTNLDLGPTAPSLEKEKTLCLSTPSSSTVKHSTSSIGSMLAQADKLPMTDKRVASLLKKAKAQLCKIEKSKSLKQTDQPKAQGQESDSSETSVRGPRIKHVCRRAAVALGRKRAVFPDDMPTLSALPWEEREKILSSMGNDDKSSIAGSEDAEPLAPPIKPIKPVTRNKAPQEPPVKKGRRSRRCGQCPGCQVPEDCGVCTNCLDKPKFGGRNIKKQCCKMRKCQNLQWMPSKAYLQKQAKAVKKKEKKSKTSEKKDSKESSVVKNVVDSSQKPTPSAREDPAPKKSSSEPPPRKPVEEKSEEGNVSAPGPESKQATTPASRKSSKQVSQPALVIPPQPPTTGPPRKEVPKTTPSEPKKKQPPPPESGPEQSKQKKVAPRPSIPVKQKPKEKEKPPPVNKQENAGTLNILSTLSNGNSSKQKIPADGVHRIRVDFKEDCEAENVWEMGGLGILTSVPITPRVVCFLCASSGHVEFVYCQVCCEPFHKFCLEENERPLEDQLENWCCRRCKFCHVCGRQHQATKQLLECNKCRNSYHPECLGPNYPTKPTKKKKVWICTKCVRCKSCGSTTPGKGWDAQWSHDFSLCHDCAKLFAKGNFCPLCDKCYDDDDYESKMMQCGKCDRWVHSKCENLSDEMYEILSNLPESVAYTCVNCTERHPAEWRLALEKELQISLKQVLTALLNSRTTSHLLRYRQAAKPPDLNPETEESIPSRSSPEGPDPPVLTEVSKQDDQQPLDLEGVKRKMDQGNYTSVLEFSDDIVKIIQAAINSDGGQPEIKKANSMVKSFFIRQMERVFPWFSVKKSRFWEPNKVSSNSGMLPNAVLPPSLDHNYAQWQEREENSHTEQPPLMKKIIPAPKPKGPGEPDSPTPLHPPTPPILSTDRSREDSPELNPPPGIEDNRQCALCLTYGDDSANDAGRLLYIGQNEWTHVNCALWSAEVFEDDDGSLKNVHMAVIRGKQLRCEFCQKPGATVGCCLTSCTSNYHFMCSRAKNCVFLDDKKVYCQRHRDLIKGEVVPENGFEVFRRVFVDFEGISLRRKFLNGLEPENIHMMIGSMTIDCLGILNDLSDCEDKLFPIGYQCSRVYWSTTDARKRCVYTCKIVECRPPVVEPDINSTVEHDENRTIAHSPTSFTESSSKESQNTAEIISPPSPDRPPHSQTSGSCYYHVISKVPRIRTPSYSPTQRSPGCRPLPSAGSPTPTTHEIVTVGDPLLSSGLRSIGSRRHSTSSLSPQRSKLRIMSPMRTGNTYSRNNVSSVSTTGTATDLESSAKVVDHVLGPLNSSTSLGQNTSTSSNLQRTVVTVGNKNSHLDGSSSSEMKQSSASDLVSKSSSLKGEKTKVLSSKSSEGSAHNVAYPGIPKLAPQVHNTTSRELNVSKIGSFAEPSSVSFSSKEALSFPHLHLRGQRNDRDQHTDSTQSANSSPDEDTEVKTLKLSGMSNRSSIINEHMGSSSRDRRQKGKKSCKETFKEKHSSKSFLEPGQVTTGEEGNLKPEFMDEVLTPEYMGQRPCNNVSSDKIGDKGLSMPGVPKAPPMQVEGSAKELQAPRKRTVKVTLTPLKMENESQSKNALKESSPASPLQIESTSPTEPISASENPGDGPVAQPSPNNTSCQDSQSNNYQNLPVQDRNLMLPDGPKPQEDGSFKRRYPRRSARARSNMFFGLTPLYGVRSYGEEDIPFYSSSTGKKRGKRSAEGQVDGADDLSTSDEDDLYYYNFTRTVISSGGEERLASHNLFREEEQCDLPKISQLDGVDDGTESDTSVTATTRKSSQIPKRNGKENGTENLKIDRPEDAGEKEHVTKSSVGHKNEPKMDNCHSVSRVKTQGQDSLEAQLSSLESSRRVHTSTPSDKNLLDTYNTELLKSDSDNNNSDDCGNILPSDIMDFVLKNTPSMQALGESPESSSSELLNLGEGLGLDSNREKDMGLFEVFSQQLPTTEPVDSSVSSSISAEEQFELPLELPSDLSVLTTRSPTVPSQNPSRLAVISDSGEKRVTITEKSVASSESDPALLSPGVDPTPEGHMTPDHFIQGHMDADHISSPPCGSVEQGHGNNQDLTRNSSTPGLQVPVSPTVPIQNQKYVPNSTDSPGPSQISNAAVQTTPPHLKPATEKLIVVNQNMQPLYVLQTLPNGVTQKIQLTSSVSSTPSVMETNTSVLGPMGGGLTLTTGLNPSLPTSQSLFPSASKGLLPMSHHQHLHSFPAATQSSFPPNISNPPSGLLIGVQPPPDPQLLVSESSQRTDLSTTVATPSSGLKKRPISRLQTRKNKKLAPSSTPSNIAPSDVVSNMTLINFTPSQLPNHPSLLDLGSLNTSSHRTVPNIIKRSKSSIMYFEPAPLLPQSVGGTAATAAGTSTISQDTSHLTSGSVSGLASSSSVLNVVSMQTTTTPTSSASVPGHVTLTNPRLLGTPDIGSISNLLIKASQQSLGIQDQPVALPPSSGMFPQLGTSQTPSTAAITAASSICVLPSTQTTGITAASPSGEADEHYQLQHVNQLLASKTGIHSSQRDLDSASGPQVSNFTQTVDAPNSMGLEQNKALSSAVQASPTSPGGSPSSPSSGQRSASPSVPGPTKPKPKTKRFQLPLDKGNGKKHKVSHLRTSSSEAHIPDQETTSLTSGTGTPGAEAEQQDTASVEQSSQKECGQPAGQVAVLPEVQVTQNPANEQESAEPKTVEEEESNFSSPLMLWLQQEQKRKESITEKKPKKGLVFEISSDDGFQICAESIEDAWKSLTDKVQEARSNARLKQLSFAGVNGLRMLGILHDAVVFLIEQLSGAKHCRNYKFRFHKPEEANEPPLNPHGSARAEVHLRKSAFDMFNFLASKHRQPPEYNPNDEEEEEVQLKSARRATSMDLPMPMRFRHLKKTSKEAVGVYRSPIHGRGLFCKRNIDAGEMVIEYAGNVIRSIQTDKREKYYDSKGIGCYMFRIDDSEVVDATMHGNAARFINHSCEPNCYSRVINIDGQKHIVIFAMRKIYRGEELTYDYKFPIEDASNKLPCNCGAKKCRKFLN paf1_human RNA polymerase II-associated factor 1 homolog PAF1 PD2 Pancreatic differentiation protein 2 protein protein human Homo sapiens Human MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMETEEKEAGGSDEEQEKGSSSEKEGSEDEHSGSESEREEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAQGGSDNDSDSGSNGGGQRSRSHSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD wdr61_human WD repeat-containing protein 61 WDR61 Meiotic recombination REC14 protein homolog SKI8 homolog protein protein human Homo sapiens Human MTNQYGILFKQEQAHDDAIWSVAWGTNKKENSETVVTGSLDDLVKVWKWRDERLDLQWSLEGHQLGVVSVDISHTLPIAASSSLDAHIRLWDLENGKQIKSIDAGPVDAWTLAFSPDSQYLATGTHVGKVNIFGVESGKKEYSLDTRGKFILSIAYSPDGKYLASGAIDGIINIFDIATGKLLHTLEGHAMPIRSLTFSPDSQLLVTASDDGYIKIYDVQHANLAGTLSGHASWVLNVAFCPDDTHFVSSSSDKSVKVWDVGTRTCVHTFFDHQDQVWGVKYNGNGSKIVSVGDDQEIHIYDCPI cdc73_human Parafibromin CDC73 C1orf28 HRPT2 Cell division cycle protein 73 homolog Hyperparathyroidism 2 protein protein protein human Homo sapiens Human MADVLSVLRQYNIQKKEIVVKGDEVIFGEFSWPKNVKTNYVVWGTGKEGQPREYYTLDSILFLLNNVHLSHPVYVRRAATENIPVVRRPDRKDLLGYLNGEASTSASIDRSAPLEIGLQRSTQVKRAADEVLAEAKKPRIEDEECVRLDKERLAARLEGHKEGIVQTEQIRSLSEAMSVEKIAAIKAKIMAKKRSTIKTDLDDDITALKQRSFVDAEVDVTRDIVSRERVWRTRTTILQSTGKNFSKNIFAILQSVKAREEGRAPEQRPAPNAAPVDPTLRTKQPIPAAYNRYDQERFKGKEETEGFKIDTMGTYHGMTLKSVTEGASARKTQTPAAQPVPRPVSQARPPPNQKKGSRTPIIIIPAATTSLITMLNAKDLLQDLKFVPSDEKKKQGCQRENETLIQRRKDQMQPGGTAISVTVPYRVVDQPLKLMPQDWDRVVAVFVQGPAWQFKGWPWLLPDGSPVDIFAKIKAFHLKYDEVRLDPNVQKWDVTVLELSYHKRHLDRPVFLRFWETLDRYMVKHKSHLRF leo1_human RNA polymerase-associated protein LEO1 LEO1 RDL Replicative senescence down-regulated leo1-like protein protein protein human Homo sapiens Human MADMEDLFGSDADSEAERKDSDSGSDSDSDQENAASGSNASGSESDQDERGDSGQPSNKELFGDDSEDEGASHHSGSDNHSERSDNRSEASERSDHEDNDPSDVDQHSGSEAPNDDEDEGHRSDGGSHHSEAEGSEKAHSDDEKWGREDKSDQSDDEKIQNSDDEERAQGSDEDKLQNSDDDEKMQNTDDEERPQLSDDERQQLSEEEKANSDDERPVASDNDDEKQNSDDEEQPQLSDEEKMQNSDDERPQASDEEHRHSDDEEEQDHKSESARGSDSEDEVLRMKRKNAIASDSEADSDTEVPKDNSGTMDLFGGADDISSGSDGEDKPPTPGQPVDENGLPQDQQEEEPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEIKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESISLAAIKNRYKGGIREERARIYSSDSDEGSEEDKAQRLLKAKKLTSDEEGEPSGKRKAEDDDKANKKHKKYVISDEEEEDDD ctr9_human RNA polymerase-associated protein CTR9 homolog CTR9 KIAA0155 SH2BP1 SH2 domain-binding protein 1 protein protein human Homo sapiens Human MSRGSIEIPLRDTDEVIELDFDQLPEGDEVISILKQEHTQLHIWIALALEYYKQGKTEEFVKLLEAARIDGNLDYRDHEKDQMTCLDTLAAYYVQQARKEKNKDNKKDLITQATLLYTMADKIIMYDQNHLLGRACFCLLEGDKMDQADAQFHFVLNQSPNNIPALLGKACISFNKKDYRGALAYYKKALRTNPGCPAEVRLGMGHCFVKLNKLEKARLAFSRALELNSKCVGALVGLAVLELNNKEADSIKNGVQLLSRAYTIDPSNPMVLNHLANHFFFKKDYSKVQHLALHAFHNTEVEAMQAESCYQLARSFHVQEDYDQAFQYYYQATQFASSSFVLPFFGLGQMYIYRGDKENASQCFEKVLKAYPNNYETMKILGSLYAASEDQEKRDIAKGHLKKVTEQYPDDVEAWIELAQILEQTDIQGALSAYGTATRILQEKVQADVPPEILNNVGALHFRLGNLGEAKKYFLASLDRAKAEAEHDEHYYNAISVTTSYNLARLYEAMCEFHEAEKLYKNILREHPNYVDCYLRLGAMARDKGNFYEASDWFKEALQINQDHPDAWSLIGNLHLAKQEWGPGQKKFERILKQPSTQSDTYSMLALGNVWLQTLHQPTRDREKEKRHQDRALAIYKQVLRNDAKNLYAANGIGAVLAHKGYFREARDVFAQVREATADISDVWLNLAHIYVEQKQYISAVQMYENCLRKFYKHQNTEVVLYLARALFKCGKLQECKQTLLKARHVAPSDTVLMFNVALVLQRLATSVLKDEKSNLKEVLNAVKELELAHRYFSYLSKVGDKMRFDLALAATEARQCSDLLSQAQYHVARARKQDEEERELRAKQEQEKELLRQKLLKEQEEKRLREKEEQKKLLEQRAQYVEKTKNILMFTGETEATKEKKRGGGGGRRSKKGGEFDEFVNDDTDDDLPISKKKKRRKGSGSEQEGEDEEGGERKKKKRRRHPKGEEGSDDDETENGPKPKKRRPPKAEKKKAPKPERLPPSMKGKIKSKAIISSSDDSSDEDKLKIADEGHPRNSNSNSDSDEDEQRKKCASSESDSDENQNKSGSEAGSPRRPRRQRSDQDSDSDQPSRKRRPSGSEQSDNESVQSGRSHSGVSENDSRPASPSAESDHESERGSDNEGSGQGSGNESEPEGSNNEASDRGSEHGSDDSD mll_af9_human_protein MLL-AF9 fusion protein protein protein human Homo sapiens Human MAHSCRWRFPARPGTTGGGGGGGRRGLGGAPRQRVPALLLPPGPPVGGGGPGAPPSPPAVAAAAAAAGSSGAGVPGGAAAASAASSSSASSSSSSSSSASSGPALLRVGPGFDAALQVSAAIGTNLRRFRAVFGESGGGGGSGEDEQFLGFGSDEEVRVRSPTRSPSVKTSPRKPRGRPRSGSDRNSAILSDPSVFSPLNKSETKSGDKIKKKDSKSIEKKRGRPPTFPGVKIKITHGKDISELPKGNKEDSLKKIKRTPSATFQQATKIKKLRAGKLSPLKSKFKTGKLQIGRKGVQIVRRRGRPPSTERIKTPSGLLINSELEKPQKVRKDKEGTPPLTKEDKTVVRQSPRRIKPVRIIPSSKRTDATIAKQLLQRAKKGAQKKIEKEAAQLQGRKVKTQVKNIRQFIMPVVSAISSRIIKTPRRFIEDEDYDPPIKIARLESTPNSRFSAPSCGSSEKSSAASQHSSQMSSDSSRSSSPSVDTSTDSQASEEIQVLPEERSDTPEVHPPLPISQSPENESNDRRSRRYSVSERSFGSRTTKKLSTLQSAPQQQTSSSPPPPLLTPPPPLQPASSISDHTPWLMPPTIPLASPFLPASTAPMQGKRKSILREPTFRWTSLKHSRSEPQYFSSAKYAKEGLIRKPIFDNFRPPPLTPEDVGFASGFSASGTAASARLFSPLHSGTRFDMHKRSPLLRAPRFTPSEAHSRIFESVTLPSNRTSAGTSSSGVSNRKRKRKVFSPIRSEPRSPSHSMRTRSGRLSSSELSPLTPPSSVSSSLSISVSPLATSALNPTFTFPSHSLTQSGESAEKNQRPRKQTSAPAEPFSSSSPTPLFPWFTPGSQTERGRNKDKAPEELSKDRDADKSVEKDKSRERDREREKENKRESRKEKRKKGSEIQSSSALYPVGRVSKEKVVGEDVATSSSAKKATGRKKSSSHDSGTDITSVTLGDTTAVKTKILIKKGRGNLEKTNLDLGPTAPSLEKEKTLCLSTPSSSTVKHSTSSIGSMLAQADKLPMTDKRVASLLKKAKAQLCKIEKSKSLKQTDQPKAQGQESDSSETSVRGPRIKHVCRRAAVALGRKRAVFPDDMPTLSALPWEEREKILSSMGNDDKSSIAGSEDAEPLAPPIKPIKPVTRNKAPQEPPVKKGRRSRRCGQCPGCQVPEDCGVCTNCLDKPKFGGRNIKKQCCKMRKCQNLQWMPSKAYLQKQAKAVKKKEKKSKTSEKKDSKESSVVKNVVDSSQKPTPSAREDPAPKKSSSEPPPRKPVEEKSEEGNVSAPGPESKQATTPASRKSSKQVSQPALVIPPQPPTTGPPRKEVPKTTPSEPKKKQPPPPESGPEQSKQKKVAPRPSIPVKQKPKEKEKPPPVNKQENAGTLNILSTLSNGNSSKQKIPADKSPIKQSKSDKQIKNGECDKAYLDELVELHRRLMTLRERHILQQIVNLIEETGHFHITNTTFDFDLCSLDKTTVRKLQSYLETSGTS An amino acid sequence which is not represented in UniProt MLL derived protein of the protein corresponds to the CxxC-RD2 portion of the parent protein, only. ccnt1_human Cyclin-T1 CCNT1 protein protein human Homo sapiens Human MEGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQRLNVSQLTINTAIVYMHRFYMIQSFTQFPGNSVAPAALFLAAKVEEQPKKLEHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESIILQTLGFELTIDHPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCIHLACKWSNWEIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPNRLKRIWNWRACEAAKKTKADDRGTDEKTSEQTILNMISQSSSDTTIAGLMSMSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRTSENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYAYAAQNLLSHHDSHSSVILKMPIEGSENPERPFLEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAADKHNSVEDSVTKSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQTSNLAHKTYSLSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSLNFSFPSLPTMGQMPGHSSDTSGLSFSQPSCKTRVPHSKLDKGPTGANGHNTTQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISSRSGNTDKPRPPPLPSEPPPPLPPLPK q6xzl8_mouse Histone-lysine N-methyltransferase, H3 lysine-79 specific Dot1l protein protein mouse Mus musculus Mouse MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENKSNATTPTKVPESKAAATEAPADSGAEEEKSGVATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMSAASAERKSKKSQSTLDLLHSPPPAPPSASPQDAYRAPHSPFYQLPPSTQLHSPNPLLVAPTPPALQKLLESFRIQYLQFLAYTKTPQYKANLQQLLDQEKEKNTQLLGTAQQLFGHCQAQKEEIRRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQSEQLEKDNSELRSQSLRLLRARCEELRLDWSTLSLENLRKEKQALRSQISEKQRHCLELQISIVELEKTQRQQELLQLKSCVPPDDALSLHLRGKGALGRELEADAGRLRLELDCAKISLPHLSSMSPELSMNGHAASYELCNAASRPSSKQNTPQYLASPLDQEVVPCTPSHSGRPRLEKLSGLALPDYTRLSPAKIVLRRHLSQDHTGASKAATSEPHPRPEHPKESSLPYQSPGLSNSMKLSPQDPPLASPATSPLTSEKGSEKGVKERAYSSHGETITSLPVSIPLSTVQPNKLPVSIPLASVVLPSRAERARSTPSPVPQPRDSSATLEKQTGASAHGAGGAGAGSRSLAVAPTGFYAGSVAISGALASSPAPLASGMESAVFDESSGPSSLFATMGSRSTPPQHPPLLSQSRNSGPASPAHQLTASPRLSVTTQGSLPDTSKGELPSDPAFSDPESEAKRRIVFSISVGASSKQSPSTRHSPLTSGTRGDCVQSHGQDSRKRSRRKRASAGTPSLSTGVSPKRRALPTVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESSLKSSPTPYQDHDQPPVLRKERPLGLTNGAHYSPLTSDEEPGSEDEPSSARIERKIATISLESKSPPKTLENGGGLVGRKSAPSSEPINSSKWKSTFSPISDLGLAKAVDSPLQAGSALSHSPLFSFRPSLEEPAAEAKLPTHPRKSFAGSLGAAEGPSPGTNPPNGLAFSGGLAADLGLHSFNDGASLSHKGPDVTGLSASLSFPSQRGKDSTTEANPFLSRRQPEGLGGLKGEGNANKESGESLPLCGPSDKASLPHGSRASKGRDRELDFKGGHNLFISAAAVPPGGLLGGPGLVTVASSAGSATPTAQAPRPFLSTFAPGPQFTLGPMSLQANLGSVAGSSVLQSLFSTVPAAAGLVHVSSTATRLTNSHTMGSFSSGVAGGTVGGN mll_enl_human_protein Fusion of human MLL (N-terminal region) with human ENL C-terminal residues 373-559 protein protein human Homo sapiens Human An amino acid sequence which is not represented in UniProt paf1_mouse RNA polymerase II-associated factor 1 homolog Paf1 protein protein mouse Mus musculus Mouse MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMEAEEKEAGGSDEEQEKGSSSEKEGSEDEHSGSESDREEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAHRGSDNDSDSGSDGGGQRSRSQSRSRSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD hoxa9_mouse_gene Mouse HOXA9 gene gene gene mouse Mus musculus Mouse leo1_mouse RNA polymerase-associated protein LEO1 Leo1 Gm185 protein protein mouse Mus musculus Mouse MADMEDLFGSEAESEAERKDSESESDSDSDQDNGASGSNASGSESDQDDRGDSGQPSNKELFGDDSEEEGASHHSGSDNHSERSDNRSEASERSDHEDNEPSDEDQHSGSEAHNDDDDEGHRSDEGSRHSEAEGSEKAQSDDEKWDGEDKSDQSDDEKLQNSDDEDREQGSDEDKLQNSDDDEEKMQNTDDEDRAQISDDDRQQLSEEEKGNSDDEHPVASDNDEEKQNSDDEDQPQVSDEEKMQNSDDERPQVSDEDGRRSDGEEEQDQKSESARGSDSEDEVLRLKRKNAIPSDSEADSDTEVPKDNNGTMDLFGGADDISSGSDGEDKPPTPGQPVDENGLPQDQQEEEPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEIKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYLEPDRYDEEEEGEESVSLAAIKNRYKGGIREERARIYSSDSDEGSEEDKAQRLLKAKKLNSDEEGESSGKRKAEDDDKANKKHKKYVISDEEEEEDD cdc73_mouse Parafibromin Cdc73 Cell division cycle protein 73 homolog Hyperparathyroidism 2 protein homolog protein protein mouse Mus musculus Mouse MADVLSVLRQYNIQKKEIVVKGDEVIFGEFSWPKNVKTNYVVWGTGKEGQPREYYTLDSILFLLNNVHLSHPVYVRRAATENIPVVRRPDRKDLLGYLNGEASTSASIDRSAPLEIGLQRSTQVKRAADEVLAEAKKPRIEDEECVRLDKERLAARLEGHKEGIVQTEQIRSLSEAMSVEKIAAIKAKIMAKKRSTIKTDLDDDITALKQRSFVDAEVDVTRDIVSRERVWRTRTTILQSTGKNFSKNIFAILQSVKAREEGRAPEQRPAPNAAPVDPTLRTKQPIPAAYNRYDQERFKGKEETEGFKIDTMGTYHGMTLKSVTEGASARKTQTPAAQPVPRPVSQARPPPNQKKGSRTPIIIIPAATTSLITMLNAKDLLQDLKFVPSDEKKKQGCQRENETLIQRRKDQMQPGGTAISVTVPYRVVDQPLKLMPQDWDRVVAVFVQGPAWQFKGWPWLLPDGSPVDIFAKIKAFHLKYDEVRLDPNVQKWDVTVLELSYHKRHLDRPVFLRFWETLDRYMVKHKSHLRF ctr9_mouse RNA polymerase-associated protein CTR9 homolog SH2 domain-binding protein 1 Tetratricopeptide repeat-containing, SH2-binding phosphoprotein of 150 kDa Ctr9 Kiaa0155 Sh2bp1 protein protein mouse Mus musculus Mouse MSRGSIEIPLRDTDEVIELDFDQLPEGDEVISILKQEHTQLHIWIALALEYYKQGKTEEFVKLLEAARIDGNLDYRDHEKDQMTCLDTLAAYYVQQARKEKNKDNKKDLITQATLLYTMADKIIMYDQNHLLGRACFCLLEGDKMDQADAQFHFVLNQSPNNIPALLGKACISFNKKDYRGALAYYKKALRTNPGCPAEVRLGMGHCFVKLNKLEKARLAFSRALELNSKCVGALVGLAVLELNNKEADSIKNGVQLLSRAYTIDPSNPMVLNHLANHFFFKKDYSKVQHLALHAFHNTEVEAMQAESCYQLARSFHVQEDYDQAFQYYYQATQFASSSFVLPFFGLGQMYIYRGDKENASQCFEKVLKAYPNNYETMKILGSLYAASEDQEKRDIAKGHLKKVTEQYPDDVEAWIELAQILEQTDIQGALSAYGTATRILQEKVQADVPPEILNNVGALHFRLGNLGEAKKYFLASLDRAKAEAEHDEHYYNAISVTTSYNLARLYEAMCEFHEAEKLYKNILREHPNYVDCYLRLGAMARDKGNFYEASDWFKEALQINQDHPDAWSLIGNLHLAKQEWGPGQKKFERILKQPATQSDTYSMLALGNVWLQTLHQPTRDREKEKRHQDRALAIYKQVLRNDAKNLYAANGIGAVLAHKGYFREARDVFAQVREATADISDVWLNLAHIYVEQKQYISAVQMYENCLRKFYKHQNTEVVLYLARALFKCGKLQECKQTLLKARHVAPSDTVLMFNVALVLQRLATSVLKDEKSNLKEVLNAVKELELAHRYFSYLSKVGDKMRFDLALAASEARQCSDLLSQAQYHVARARKQDEEERELRAKQEQEKELLRQKLLKEQEEKRLREKEEQKKLLEQRAQYVEKTKNILMFTGETEATKEKKRGGGGGRRSKKGGEFDEFVNDDTDDDLPVSKKKKRRKGSGSEQEGEEEEGGERKKKRRRRPPKGEEGSEEEETENGPKPKKRRPPRAEKKKAPKPERLPPSMKGKIKSKAIISSSDDSSDEDKLKIADEGHPRNSNSDSDDDERPNRRASSESDSDDNQNKSGSEAGSPRRSGRQESDEDSDSDQPSRKRRRSGSEQSDNESVQSGRSPSGASENENDSRPASPSAESDHESEQGSDNEGSGQGSGNESEPEGSNNEASDRGSEHGSDDSD q8cj76_mouse Mllt1 protein protein mouse Mus musculus Mouse MDNQCTVQVKLELGHRAQLRKKPTTEGFTHDWMVFVRGPEQCDIQHFVEKVIFRLHDSFPKPKRVCKEPPYKVEESGYAGFIMLIEVYFKNKEEPRKVCFTYDLFLNLEGNPPVNHLRCEKLTFNNPTTEFRCKLLMAGGVMVMPEGADTVSRPSPDYPMLPTIPLSAFSDPKKNKPSHGSKDANKESGKASKPHKVAREHRERPRKDSESRSCSKEPEREPKSAKDAARKLPKEEKAPVPKAAFKEPKMALKETKLESLSPKGVPQPPTLPKASSKRPAATDSPKLSAKKPKKSGSKGTRRAPGTSPRASSFPDKKVARDKNSKGDKGKAESEPREAKRTLQTEDSNSEDEASFRSESAQSSPANASSSSDSSSDSDFEPSQNHSQGPLRSMVEDLQSEGSDEDDSSSGEENTRKANPGRESRLSFSDSESDNSADSCLPGREPLPPQKPPPPSSKVSGRRSPEPCSKPEKMLKKATYDKAYTDELVELHRRLMALRERNVLQQIVNLIEETGHFNVTNTTFDFDLFFLDESTVRKLQSCLEAVAT hoxa9_human_gene Human HOXA9 gene gene gene human Homo sapiens Human enl_human Protein ENL MLLT1 ENL LTG19 YEATS1 YEATS domain-containing protein 1 protein protein human Homo sapiens Human MDNQCTVQVRLELGHRAQLRKKPTTEGFTHDWMVFVRGPEQCDIQHFVEKVVFWLHDSFPKPRRVCKEPPYKVEESGYAGFIMPIEVHFKNKEEPRKVCFTYDLFLNLEGNPPVNHLRCEKLTFNNPTTEFRYKLLRAGGVMVMPEGADTVSRPSPDYPMLPTIPLSAFSDPKKTKPSHGSKDANKESSKTSKPHKVTKEHRERPRKDSESKSSSKELEREQAKSSKDTSRKLGEGRLPKEEKAPPPKAAFKEPKMALKETKLESTSPKGGPPPPPPPPPRASSKRPATADSPKPSAKKQKKSSSKGSRSAPGTSPRTSSSSSFSDKKPAKDKSSTRGEKVKAESEPREAKKALEVEESNSEDEASFKSESAQSSPSNSSSSSDSSSDSDFEPSQNHSQGPLRSMVEDLQSEESDEDDSSSGEEAAGKTNPGRDSRLSFSDSESDNSADSSLPSREPPPPQKPPPPNSKVSGRRSPESCSKPEKILKKGTYDKAYTDELVELHRRLMALRERNVLQQIVNLIEETGHFNVTNTTFDFDLFSLDETTVRKLQSCLEAVAT q2q440_human protein protein human Homo sapiens Human FLCASSGHVEDANKESSKTS cxxc-rd2-1 959541 n/a 959548 unspecified role unspecified role bait bait cxxc_rd2_ domain CxxC-RD2 region including a nuclear localization signal (NLS). sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 959549 unspecified role unspecified role prey prey n/a 959550 unspecified role unspecified role prey prey n/a 959551 unspecified role unspecified role prey prey n/a 959552 unspecified role unspecified role prey prey n/a 959553 unspecified role unspecified role prey prey association association false false false Fig 1C, S1B. Immunoprecipitation was preserved in the presence of Benzonase indicating the interaction is not DNA dependent. mll_af9-wdr61 959541 n/a 959554 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 959553 unspecified role unspecified role prey prey n/a 959552 unspecified role unspecified role prey prey n/a 959551 unspecified role unspecified role prey prey n/a 959549 unspecified role unspecified role prey prey n/a 959550 unspecified role unspecified role prey prey association association false false false Fig 1D. The lysate was treated with benzonase to digest DNA, indicating the PAFc interaction with CxxC-RD2 is DNA independent. Myc-tagged MLL CxxC-RD2-AF9 (MLL amino acids 1116-1397; AF9 - C terminal amino acids 276-568) interacts with PAFc subunits, showing that PAFc binding to CxxC-RD2 is maintained in the presence of the AF9 translocation partner. mll_af9-ctr9-1 959541 n/a 959554 unspecified role unspecified role bait bait n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 959551 unspecified role unspecified role prey prey n/a 959549 unspecified role unspecified role prey prey n/a 959553 unspecified role unspecified role prey prey n/a 959550 unspecified role unspecified role prey prey association association false false false Fig 1E. These experiments show the MLL-PAFc interaction is maintained both in the context of full-length (MLLN) as well as in the context of a leukemogenic MLL fusion protein. (MLL amino acids 1-1396;AF9 C terminal amino acids 276-568). mll-ctr9-3 959541 n/a 959550 unspecified role unspecified role prey prey n/a 959553 unspecified role unspecified role prey prey n/a 959548 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 959549 unspecified role unspecified role prey prey association association false false false Fig 1F. These experiments show the MLL-PAFc interaction is maintained both in the context of full-length (MLLN) as well as in the context of a leukemogenic MLL fusion protein. (MLL amino acids 1-1396;AF9 C terminal amino acids 276-568). mll_af9-ctr9 959541 n/a 959549 unspecified role unspecified role prey prey n/a 959551 unspecified role unspecified role prey prey n/a 959553 unspecified role unspecified role prey prey n/a 959554 unspecified role unspecified role bait bait n terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 3B, S4A. Using (N-terminal) Myc-tagged MLL CxxC-RD2-(C terminal)AF9 (C terminal amino acids 276-568) deletion mutants there was a sharp decrease in the MLL-PAFc interaction when C-terminal deletions were made past amino acid 1299. Residual low level binding of PAFc with proteins deleted at aa 1209 or aa 1258 was detected. mll_af9-leo1 959541 n/a 959550 unspecified role unspecified role prey prey n/a 959551 unspecified role unspecified role prey prey n/a 959549 unspecified role unspecified role prey prey n/a 959552 unspecified role unspecified role prey prey n/a 959554 unspecified role unspecified role bait bait n terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 3D, S4A. Experiments using deletion constructs [(N-terminal) Myc-tagged MLL CxxC-RD2-(C terminal)AF9 (C terminal amino acids 276-568)] that begin with MLL aa 1180 (deletion of the proximal site of PAFc interaction) showed PAFc interaction with MLL is completely eliminated with deletions beyond aa 1299. mll_af9-dot1l-cyt1 959541 n/a 959555 unspecified role unspecified role prey prey n/a 959554 unspecified role unspecified role bait bait n terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 959556 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig S4A. Experiments using deletion constructs [(N-terminal) Myc-tagged MLL CxxC-RD2-(C terminal)AF9 (C terminal amino acids 276-568)] that begin with MLL aa 1180 (deletion of the proximal site of PAFc interaction) showed PAFc interaction with MLL is completely eliminated with deletions beyond aa 1299. Association of DOT1L and the pTEFb component, cyclin T1, with AF9 was maintained in all the deletion constructs used. hoxa9-paf1-1 959542 n/a 959557 unspecified role unspecified role ancillary ancillary n/a 959558 unspecified role unspecified role bait bait n/a 959559 unspecified role unspecified role prey prey association association false false false Fig 6B. Co-localization of MLL-ENL and PAFc at the Meis1 and Hoxa9 loci in MLL-ENL transfected cells, with minimal binding seen in E2A-HLF cells. MLL-ENL binding is somewhat more abundant upstream and downstream of the transcriptional start site of the Hoxa9 locus. hoxa9-leo1 959542 n/a 959560 unspecified role unspecified role bait bait n/a 959559 unspecified role unspecified role prey prey n/a 959557 unspecified role unspecified role ancillary ancillary association association false false false Fig 6B. Co-localization of MLL-ENL and PAFc at the Meis1 and Hoxa9 loci in MLL-ENL transfected cells, with minimal binding seen in E2A-HLF cells. MLL-ENL binding is somewhat more abundant upstream and downstream of the transcriptional start site of the Hoxa9 locus. hoxa9-cdc73 959542 n/a 959561 unspecified role unspecified role bait bait n/a 959559 unspecified role unspecified role prey prey n/a 959557 unspecified role unspecified role ancillary ancillary association association false false false Fig 6B. Co-localization of MLL-ENL and PAFc at the Meis1 and Hoxa9 loci in MLL-ENL transfected cells, with minimal binding seen in E2A-HLF cells. MLL-ENL binding is somewhat more abundant upstream and downstream of the transcriptional start site of the Hoxa9 locus. hoxa9-ctr9 959542 n/a 959562 unspecified role unspecified role bait bait n/a 959557 unspecified role unspecified role ancillary ancillary n/a 959559 unspecified role unspecified role prey prey association association false false false Fig 6B. Co-localization of MLL-ENL and PAFc at the Meis1 and Hoxa9 loci in MLL-ENL transfected cells, with minimal binding seen in E2A-HLF cells. MLL-ENL binding is somewhat more abundant upstream and downstream of the transcriptional start site of the Hoxa9 locus. hoxa9-enl 959542 n/a 959559 unspecified role unspecified role prey prey n/a 959563 unspecified role unspecified role bait bait n/a 959557 unspecified role unspecified role ancillary ancillary association association false false false Fig 6B. Co-localization of MLL-ENL and PAFc at the Meis1 and Hoxa9 loci in MLL-ENL transfected cells, with minimal binding seen in E2A-HLF cells. MLL-ENL binding is somewhat more abundant upstream and downstream of the transcriptional start site of the Hoxa9 locus. hoxa9-paf1 959543 n/a 959549 unspecified role unspecified role bait bait n/a 959564 unspecified role unspecified role prey prey association association false false false Fig 7D. A significant decrease in binding was observed of CDC73, PAF1, and LEO1 in both the promoter and coding region of the HOXA9 locus following knockdown of PAF complex components. hox9a-cdc73 959543 n/a 959551 unspecified role unspecified role bait bait n/a 959564 unspecified role unspecified role prey prey association association false false false Fig 7D. A significant decrease in binding was observed of CDC73, PAF1, and LEO1 in both the promoter and coding region of the HOXA9 locus following knockdown of PAF complex components. hox9a-leo1 959543 n/a 959564 unspecified role unspecified role prey prey n/a 959552 unspecified role unspecified role bait bait association association false false false Fig 7D. A significant decrease in binding was observed of CDC73, PAF1, and LEO1 in both the promoter and coding region of the HOXA9 locus following knockdown of PAF complex components. hox9a-mllc 959543 n/a 959548 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 959564 unspecified role unspecified role prey prey association association false false false Fig 7D. PAFc knockdown resulted in a marked decrease in wild-type MLL binding compared with mock treated cells. mll-paf 959544 n/a 959548 unspecified role unspecified role bait bait cxxc_rd2_ domain CxxC-RD2 region including a nuclear localization signal (NLS). sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false his-mocr tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 959549 unspecified role unspecified role prey prey region His-MOCR tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 2C. PAFc co-immunoprecipitated with a small fragment of MLL N-terminal to the CxxC domain (amino acids 1115-1154). MLL CxxC-RD2 and RD2 regions specifically associated with CTR9-C, and with a weak interaction detected between RD2 and CDC73. mll-cdc73 959544 n/a 959551 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 959548 unspecified role unspecified role bait bait rd2_ domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region His-MOCR tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 2C. PAFc co-immunoprecipitated with a small fragment of MLL N-terminal to the CxxC domain (amino acids 1115-1154). MLL CxxC-RD2 and RD2 regions specifically associated with CTR9-C, and with a weak interaction detected between RD2 and CDC73. mll-ctr9-2 959544 n/a 959553 unspecified role unspecified role prey prey c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 959548 unspecified role unspecified role bait bait region His-MOCR tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rd2_ domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 959669 959664 physical association physical association false false false Fig 2C. PAFc co-immunoprecipitated with a small fragment of MLL N-terminal to the CxxC domain (amino acids 1115-1154). MLL CxxC-RD2 and RD2 regions specifically associated with CTR9-C, and with a weak interaction detected between RD2 and CDC73. rd2-ctr9-1 959545 n/a 959548 unspecified role unspecified role prey prey his-mocr tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rd2_ domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 959553 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 959675 959673 physical association physical association false false false Fig S2 A+B. PAFc co-immunoprecipitated with a small fragment of MLL N-terminal to the CxxC domain (amino acids 1115-1154). MLL CxxC-RD2 and RD2 regions specifically associated with CTR9-C, and with a weak interaction detected between RD2 and CDC73. rd2-cdc73-1 959545 n/a 959548 unspecified role unspecified role prey prey rd2_ domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false his-mocr tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 959551 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig S2 cxxc-rd2 959546 n/a 959551 unspecified role unspecified role prey prey n/a 959549 unspecified role unspecified role prey prey n/a 959552 unspecified role unspecified role prey prey n/a 959553 unspecified role unspecified role prey prey n/a 959548 unspecified role unspecified role bait bait cxxc_rd2_ domain CxxC-RD2 region including a nuclear localization signal (NLS). sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 959550 unspecified role unspecified role prey prey association association false false false Fig 1B. enl-cdc73 959547 n/a 959565 unspecified role unspecified role bait bait n/a 959551 unspecified role unspecified role prey prey n/a 959566 unspecified role unspecified role prey prey physical association physical association false false false Fig S1C. Immunoprecipitation of endogenous MLL-ENL from KOPN8 cells and western blotting for the PAF component CDC73 shows interaction of endogenous MLL-ENL with endogenous PAFc.