IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ adorno-2009-1 A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. human-hacat Homo sapiens immortalised keratinocytes hacat human immortalised keratinocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Cell (0092-8674) 2009 Only protein-protein interactions 10 imex curation Cancer - Interactions investigated in the context of cancer adorno-2009-4 A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Cell (0092-8674) 2009 Only protein-protein interactions 10 imex curation Cancer - Interactions investigated in the context of cancer adorno-2009-2 A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Cell (0092-8674) 2009 Only protein-protein interactions 10 imex curation Cancer - Interactions investigated in the context of cancer adorno-2009-3 A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Cell (0092-8674) 2009 Only protein-protein interactions 10 imex curation Cancer - Interactions investigated in the context of cancer adorno-2009-6 A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Cell (0092-8674) 2009 Only protein-protein interactions 10 imex curation Cancer - Interactions investigated in the context of cancer adorno-2009-7 A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. mouse-d3 Mouse D3 spindle cells d3 Mouse D3 spindle cell carcinoma of the skin cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Cell (0092-8674) 2009 Only protein-protein interactions 10 imex curation Cancer - Interactions investigated in the context of cancer adorno-2009-8 A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adorno M., Cordenonsi M., Montagner M., Dupont S., Wong C., Hann B., Solari A., Bobisse S., Rondina MB., Guzzardo V., Parenti AR., Rosato A., Bicciato S., Balmain A., Piccolo S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of SMAD3 previously communicated by authors (EBI-2214463), origin of p63 taken from publication of donating author PMID:12446784 piccolo@civ.bio.unipd.it Cell (0092-8674) 2009 Only protein-protein interactions 10 imex curation Cancer - Interactions investigated in the context of cancer p63_human Tumor protein 63 TP63 KET P63 P73H P73L TP73L Transformation-related protein 63 Tumor protein p73-like p51 p40 Keratinocyte transcription factor KET Chronic ulcerative stomatitis protein protein protein human Homo sapiens Human MNFETSRCATLQYCPDPYIQRFVETPAHFSWKESYYRSTMSQSTQTNEFLSPEVFQHIWDFLEQPICSVQPIDLNFVDEPSEDGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSTKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD smad2_human Mothers against decapentaplegic homolog 2 SMAD2 MADH2 MADR2 Mad-related protein 2 SMAD family member 2 JV18-1 protein protein human Homo sapiens Human MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS smad3_human Mothers against decapentaplegic homolog 3 SMAD3 MADH3 SMAD family member 3 JV15-2 protein protein human Homo sapiens Human MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS o88898-5 Tumor protein 63 Tp63 P63 P73l Tp73l Trp63 Transformation-related protein 63 Tumor protein p73-like TA*-gamma TA*p63gamma protein protein mouse Mus musculus Mouse MNFETSRCATLQYCPDPYIQRFIETPAHFSWKESYYRSAMSQSTQTSEFLSPEVFQHIWDFLEQPICSVQPIELNFVDEPSENGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSAKNGDAFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKHLLSACFRNELVEPRGEAPTQSDVFFRHSNPPNHSVYP Produced by alternative splicing of isoform 1. o88898-2 Tumor protein 63 Tp63 P63 P73l Tp73l Trp63 Transformation-related protein 63 Tumor protein p73-like DeltaN-alpha protein protein mouse Mus musculus Mouse MLYLENNAQTQFSEPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSAKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSMQSQSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTMPEGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHDFSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE Produced by alternative promoter usage. smad3_mouse Mothers against decapentaplegic homolog 3 Smad3 Madh3 SMAD family member 3 protein protein mouse Mus musculus Mouse MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS p63_mouse Tumor protein 63 Tp63 P63 P73l Tp73l Trp63 Transformation-related protein 63 Tumor protein p73-like protein protein mouse Mus musculus Mouse MNFETSRCATLQYCPDPYIQRFIETPAHFSWKESYYRSAMSQSTQTSEFLSPEVFQHIWDFLEQPICSVQPIELNFVDEPSENGATNKIEISMDCIRMQDSDLSDPMWPQYTNLGLLNSMDQQIQNGSSSTSPYNTDHAQNSVTAPSPYAQPSSTFDALSPSPAIPSNTDYPGPHSFDVSFQQSSTAKSATWTYSTELKKLYCQIAKTCPIQIKVMTPPPQGAVIRAMPVYKKAEHVTEVVKRCPNHELSREFNEGQIAPPSHLIRVEGNSHAQYVEDPITGRQSVLVPYEPPQVGTEFTTVLYNFMCNSSCVGGMNRRPILIIVTLETRDGQVLGRRCFEARICACPGRDRKADEDSIRKQQVSDSAKNGDGTKRPFRQNTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSMQSQSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTMPEGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHDFSSPPHLLRTPSGASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDSRRNKQQRIKEEGE smad2_mouse Mothers against decapentaplegic homolog 2 Smad2 Madh2 Madr2 Mad-related protein 2 SMAD family member 2 protein protein mouse Mus musculus Mouse MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTANTVDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human tp53-tp63-1 Interaction between TP53 and TP63 identified by coimmunopreciptiation 814145 n/a 814152 unspecified role unspecified role prey prey n/a 814153 unspecified role unspecified role bait bait P04637:p.[His179Tyr;Arg282Trp] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false P04637 transfection Fig. 4A TGF-beta Pre-treatment with anti-SMAD2/3 or anti-TP53 siRNA inhibited this interaction. tp53-tp63-2 Interaction between TP53, SMAD2 and TP63 identified by coimmunopreciptiation 814145 n/a 814153 unspecified role unspecified role prey prey P04637:p.[His179Tyr;Arg282Trp] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 814152 unspecified role unspecified role prey prey n/a 814154 unspecified role unspecified role bait bait physical association physical association false false false P04637 transfection Fig. 4B TGF-beta Pre-treatment with anti-SMAD2/3 or anti-TP63 siRNA inhibited this interaction. smad2-tp63-1 Interaction between SMAD2 and TP63 identified by coimmunopreciptiation 814145 n/a 814152 unspecified role unspecified role prey prey n/a 814154 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4B TGF-beta Cells were pre-treated with anti-TP53 siRNA. smad3-tp63-1 Interaction between SMAD3 and p63 demonstrated by pulldown 814146 n/a 814155 unspecified role unspecified role bait bait m1 region required to bind necessary binding region required to bind range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 814156 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ta region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro 814180 814176 direct interaction direct interaction false false false Exact coordinates of the SMAD3 M1 are not given in the text so have been inferred from the UniProtKB entry. Fig. 5A, Supp Fi. 7 smad3-tp63-2 Interaction between SMAD3 and p63 demonstrated by pulldown 814146 n/a 814157 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 814155 unspecified role unspecified role bait bait m2 region required to bind necessary binding region required to bind range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Exact coordinates of the SMAD3 M2 are not given in the text so have been inferred from the UniProtKB entry. Fig. 5A, Supp Fi. 7 tp53-tp63-3 Interaction between TP53, SMAD2, SMAD3 and TP63 identified by coimmunopreciptiation 814147 n/a 814158 unspecified role unspecified role prey prey n/a 814159 unspecified role unspecified role prey prey n/a 814160 unspecified role unspecified role prey prey n/a 814153 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false P04637 transfection Fig. 4C TGF-beta TGF-beta induced the formation of a complex between p63, mutant-p53 and Smads only in presence of oncogenic Ras. TGF-beta receptor inhibitor SB431542 tp53-tp63-4 Interaction between TP53, SMAD2 and TP63 identified by coimmunopreciptiation 814148 n/a 814153 unspecified role unspecified role bait bait P04637:p.Arg280Lys mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 814152 unspecified role unspecified role prey prey n/a 814154 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4E, Supp. Fig. 5B TGF-beta Pre-treatment with anti-SMAD2/3, anti CK1 epsilon/delta or anti-TP53 siRNA inhibited this interaction, as did inhibiting the RAS pathway with PD98059. cdkn1a-1 814149 n/a 814161 unspecified role unspecified role prey prey n/a 814152 unspecified role unspecified role bait bait association association false false false Fig. 5D Interaction is inhibited by p53 (arg273his) but only when cells are treated with TGF-beta. tp53-tp63-5 Interaction between Tp53 and Tp63 identified by coimmunopreciptiation 814150 n/a 814159 unspecified role unspecified role prey prey n/a 814153 unspecified role unspecified role bait bait P04637:p.Cys238Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Interaction also occurs in a metastatic sub-popultation of cells, D3S2. The interaction was not observed following treatment with anti SMAD2/3 siRNA. TGF-beta Fig. 6B tp53-tp63-6 Interaction between TP53, SMAD2 and TP63 identified by coimmunopreciptiation 814151 n/a 814152 unspecified role unspecified role prey prey n/a 814153 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 814154 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 5C TGF-beta Mutation of the Ras/CK1-targeted phosphorylation sites of mutant-p53 abolished ternary complex formation.