IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ weihua-2008-1 Survival of cancer cells is maintained by EGFR independent of its kinase activity. human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weihua Z., Tsan R., Huang WC., Wu Q., Chiu CH., Fidler IJ., Hung MC. Cancer - Interactions investigated in the context of cancer The PC-3MM2 cell line, a metastatic prostate cancer cell line derived from the parental PC3 line, was used. mhung@mdanderson.org,ifidler@mdanderson.org Cancer Cell (1535-6108) 2008 Only protein-protein interactions 3 imex curation weihua-2008-2 Survival of cancer cells is maintained by EGFR independent of its kinase activity. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weihua Z., Tsan R., Huang WC., Wu Q., Chiu CH., Fidler IJ., Hung MC. Cancer - Interactions investigated in the context of cancer The PC-3MM2 cell line, a metastatic prostate cancer cell line derived from the parental PC3 line, was used. The source of EGFR was taken from PMID 2790960, (a paper referred to by the reference CHan and Gill (1996) given by the authors). mhung@mdanderson.org,ifidler@mdanderson.org Cancer Cell (1535-6108) 2008 Only protein-protein interactions 3 imex curation weihua-2008-3 Survival of cancer cells is maintained by EGFR independent of its kinase activity. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Weihua Z., Tsan R., Huang WC., Wu Q., Chiu CH., Fidler IJ., Hung MC. Cancer - Interactions investigated in the context of cancer The PC-3MM2 cell line, a metastatic prostate cancer cell line derived from the parental PC3 line, was used. The source of EGFR was taken from PMID 2790960, (a paper referred to by the reference Chan and Gill (1996) given by the authors). The ranges of the EGFR ECD and TM domains construct and the ICD construct were taken from the paper referred to by the authors (PMID 17283074; Hsu and Hung, 2007). mhung@mdanderson.org,ifidler@mdanderson.org Cancer Cell (1535-6108) 2008 Only protein-protein interactions 3 imex curation egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA sc5a1_human Sodium/glucose cotransporter 1 SLC5A1 NAGT SGLT1 High affinity sodium-glucose cotransporter Solute carrier family 5 member 1 protein protein human Homo sapiens Human MDSSTWSPKTTAVTRPVETHELIRNAADISIIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPIGASLFASNIGSGHFVGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQRIQVYLSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTDTLQTVIMLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFIFGMSILTLWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLMPMFIMVMPGMISRILYTEKIACVVPSECEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIYAKVRKRASEKELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLILGLLIGISRMITEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISFITIVVISLLTKPIPDVHLYRLCWSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRTVLNVNGIILVTVAVFCHAYFA egfr-slc5a1 EGFR coimmunoprecipitates SLC5A1 (SLGT1) in PC3MM2 lysates 736470 N/A 736473 unspecified role unspecified role bait bait N/A 736474 unspecified role unspecified role prey prey physical association physical association false false false 5 D This interaction was independent of phosphorylation of EGFR. egfr-slc5a1-1 EGFR coimmunoprecipitates SLC5A1 (SLGT1) in MCF-7 lysates 736471 N/A 736473 unspecified role unspecified role bait bait N/A 736474 unspecified role unspecified role prey prey physical association physical association false false false 5 E P13866; P00533 transfection egfr-slc5a1-2 EGFR coimmunoprecipitates SLC5A1 (SLGT1) in HEK293 lysates 736472 N/A 736473 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal ECD plus TM required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 736474 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 5 F P13866; P00533 transfection SLC5A1 (SLGT1) coimmunoprecipitates with the EGFR extracellular domain (ECD) plus transmembrane doamin aa 1-648 but not the intracellular domain (ICD) aa 640-1186.