IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ iorns-2008-3 Identification of CDK10 as an Important Determinant of Resistance to Endocrine Therapy for Breast Cancer. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Iorns E., Turner NC., Elliott R., Syed N., Garrone O., Gasco M., Tutt AN., Crook T., Lord CJ., Ashworth A. Cancer - Interactions investigated in the context of cancer The product number provided by the authors was used to search the Genecopoeia website, which shows the CDK10 construct used to be of human origin. chris.lord@icr.ac.uk,alan.ashworth@icr.ac.uk Cancer Cell (1535-6108) 2008 Only protein-protein interactions 2 imex curation iorns-2008-1 Identification of CDK10 as an Important Determinant of Resistance to Endocrine Therapy for Breast Cancer. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Iorns E., Turner NC., Elliott R., Syed N., Garrone O., Gasco M., Tutt AN., Crook T., Lord CJ., Ashworth A. Cancer - Interactions investigated in the context of cancer The product number provided by the authors was used to search the Genecopoeia website, which shows the CDK10 construct used to be of human origin. chris.lord@icr.ac.uk,alan.ashworth@icr.ac.uk Cancer Cell (1535-6108) 2008 Only protein-protein interactions 2 imex curation iorns-2008-2 Identification of CDK10 as an Important Determinant of Resistance to Endocrine Therapy for Breast Cancer. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Iorns E., Turner NC., Elliott R., Syed N., Garrone O., Gasco M., Tutt AN., Crook T., Lord CJ., Ashworth A. Cancer - Interactions investigated in the context of cancer The product number provided by the authors was used to search the Genecopoeia website, which shows the CDK10 construct used to be of human origin. chris.lord@icr.ac.uk,alan.ashworth@icr.ac.uk Cancer Cell (1535-6108) 2008 Only protein-protein interactions 2 imex curation cdk10_human Cyclin-dependent kinase 10 CDK10 Serine/threonine-protein kinase PISSLRE Cell division protein kinase 10 protein protein human Homo sapiens Human MAEPDLECEQIRLKCIRKEGFFTVPPEHRLGRCRSVKEFEKLNRIGEGTYGIVYRARDTQTDEIVALKKVRMDKEKDGIPISSLREITLLLRLRHPNIVELKEVVVGNHLESIFLVMGYCEQDLASLLENMPTPFSEAQVKCIVLQVLRGLQYLHRNFIIHRDLKVSNLLMTDKGCVKTADFGLARAYGVPVKPMTPKVVTLWYRAPELLLGTTTQTTSIDMWAVGCILAELLAHRPLLPGTSEIHQIDLIVQLLGTPSENIWPGFSKLPLVGQYSLRKQPYNNLKHKFPWLSEAGLRLLHFLFMYDPKKRATAGDCLESSYFKEKPLPCEPELMPTFPHHRNKRAAPATSEGQSKRCKP raf1_human_gene Human RAF1 (c-RAF) gene gene gene human Homo sapiens Human ets2_human Protein C-ets-2 ETS2 protein protein human Homo sapiens Human MNDFGIKNMDQVAPVANSYRGTLKRQPAFDTFDGSLFAVFPSLNEEQTLQEVPTGLDSISHDSANCELPLLTPCSKAVMSQALKATFSGFKKEQRRLGIPKNPWLWSEQQVCQWLLWATNEFSLVNVNLQRFGMNGQMLCNLGKERFLELAPDFVGDILWEHLEQMIKENQEKTEDQYEENSHLTSVPHWINSNTLGFGTEQAPYGMQTQNYPKGGLLDSMCPASTPSVLSSEQEFQMFPKSRLSSVSVTYCSVSQDFPGSNLNLLTNNSGTPKDHDSPENGADSFESSDSLLQSWNSQSSLLDVQRVPSFESFEDDCSQSLCLNKPTMSFKDYIQERSDPVEQGKPVIPAAVLAGFTGSGPIQLWQFLLELLSDKSCQSFISWTGDGWEFKLADPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTSGKRYVYRFVCDLQNLLGFTPEELHAILGVQPDTED cdk10-raf1_human HA-CDK10 binds to the c-RAF promoter as shown by a ChIP asay 729160 N/A 729163 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 729164 unspecified role unspecified role prey prey association association false false false 7 F Q15131 transfection CDK10 binds to the ETS2-binding site in the c-RAF promoter (GGAT). ets2-raf1_human-1 ETS2 binds to the c-RAF promoter as shown by a ChIP asay 729160 N/A 729164 unspecified role unspecified role prey prey N/A 729165 unspecified role unspecified role bait bait association association false false false 7 F, G Q15131 transfection Silencing of CDK10 leads to enhanced binding of ETS2 to the ETS2 binding site in the c-RAF promoter. CDK10 - Transfection with the CDK10 expression vector decreases this interaction. ETS2 binds to the ETS2-binding site in the c-RAF promoter (GGAT). cdk10-ets2 HA-CDK10 coimmunoprecipitates endogenous ETS2 in MCF7 lysates 729161 N/A 729163 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 729165 unspecified role unspecified role prey prey physical association physical association false false false 7 D Q15131 transfection ets2-cdk10 Endogenous CDK10 coimmunoprecipitates with endogenous ETS2 in MCF7 lysates 729162 N/A 729163 unspecified role unspecified role prey prey N/A 729165 unspecified role unspecified role bait bait physical association physical association false false false S5