IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ simova-2007-3 Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Simova S., Klima M., Cermak L., Sourkova V., Andera L. Apoptosis - Interactions involving proteins with a function related to apoptosis Supp. Fig 2B could not be opened for curation as the available file appeared to be corrupted. From comparing the authors given range for the intracellular part of DR4 (aa 246-445), it is clear that they have not included the signal peptide of 23 aa that is included in the UniProtKB entry (O00220) for DR4. Thus the range has been altered by 23 to compensate for this discrepancy. andera@img.cas.cz. HA-tagged human cDNA libraries (leukocyte/bone marrow) were used. Apoptosis (1360-8185) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer simova-2007-1 Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Simova S., Klima M., Cermak L., Sourkova V., Andera L. Apoptosis - Interactions involving proteins with a function related to apoptosis Supp. Fig 2B could not be opened for curation as the available file appeared to be corrupted. From comparing the authors given range for the intracellular part of DR4 (aa 246-445), it is clear that they have not included the signal peptide of 23 aa that is included in the UniProtKB entry (O00220) for DR4. Thus the range has been altered by 23 to compensate for this discrepancy. andera@img.cas.cz. HA-tagged human cDNA libraries (leukocyte/bone marrow) were used. Apoptosis (1360-8185) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer simova-2007-2 Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Simova S., Klima M., Cermak L., Sourkova V., Andera L. Apoptosis - Interactions involving proteins with a function related to apoptosis Supp. Fig 2B could not be opened for curation as the available file appeared to be corrupted. From comparing the authors given range for the intracellular part of DR4 (aa 246-445), it is clear that they have not included the signal peptide of 23 aa that is included in the UniProtKB entry (O00220) for DR4. Thus the range has been altered by 23 to compensate for this discrepancy. andera@img.cas.cz. HA-tagged human cDNA libraries (leukocyte/bone marrow) were used. Apoptosis (1360-8185) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer simova-2007-4 Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. human-nctc Homo sapiens skin epithelial-like cells nctc Human skin epithelial-like cells confocal microscopy confocal microscopy immunostaining immunostaining Simova S., Klima M., Cermak L., Sourkova V., Andera L. Apoptosis - Interactions involving proteins with a function related to apoptosis Supp. Fig 2B could not be opened for curation as the available file appeared to be corrupted. From comparing the authors given range for the intracellular part of DR4 (aa 246-445), it is clear that they have not included the signal peptide of 23 aa that is included in the UniProtKB entry (O00220) for DR4. Thus the range has been altered by 23 to compensate for this discrepancy. andera@img.cas.cz. HA-tagged human cDNA libraries (leukocyte/bone marrow) were used. Apoptosis (1360-8185) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer arap1_human Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 ARAP1 CENTD2 Centaurin-delta-2 KIAA0782 protein protein human Homo sapiens Human MAEAGDAALSVAEWLRALHLEQYTGLFEQHGLVWATECQGLSDTRLMDMGMLLPGHRRRILAGLLRAHTSPAPAPRPTPRPVPMKRHIFRSPPVPATPPEPLPTTTEDEGLPAAPPIPPRRSCLPPTCFTTPSTAAPDPVLPPLPAKRHLAELSVPPVPPRTGPPRLLVSLPTKEEESLLPSLSSPPQPQSEEPLSTLPQGPPQPPSPPPCPPEIPPKPVRLFPEFDDSDYDEVPEEGPGAPARVMTKKEEPPPSRVPRAVRVASLLSEGEELSGDDQGDEEEDDHAYEGVPNGGWHTSSLSLSLPSTIAAPHPMDGPPGGSTPVTPVIKAGWLDKNPPQGSYIYQKRWVRLDTDHLRYFDSNKDAYSKRFISVACISHVAAIGDQKFEVITNNRTFAFRAESDVERKEWMQALQQAMAEQRARARLSSAYLLGVPGSEQPDRAGSLELRGFKNKLYVAVVGDKVQLYKNLEEYHLGIGITFIDMSVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAERIWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTETLIELFLQLGNGAGNRFWAANVPPSEALQPSSSPSTRRCHLEAKYREGKYRRYHPLFGNQEELDKALCAAVTTTDLAETQALLGCGAGINCFSGDPEAPTPLALAEQAGQTLQMEFLRNNRTTEVPRLDSMKPLEKHYSVVLPTVSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGVLSYFENERAVTPNGEIRASEIVCLAVPPPDTHGFEHTFEVYTEGERLYLFGLESAEQAHEWVKCIAKAFVPPLAEDLLARDFERLGRLPYKAGLSLQRAQEGWFSLSGSELRAVFPEGPCEEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMGWLGAIQKAAASMGDTLSEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLESLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQRLTWLEASEIEDEEEKVSRYRELLVRLPPVNRATVKALISHLYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSQRPWSGAPETSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV tr10a_human Tumor necrosis factor receptor superfamily member 10A Death receptor 4 TNF-related apoptosis-inducing ligand receptor 1 TNFRSF10A APO2 DR4 TRAILR1 protein protein human Homo sapiens Human MAPPPARVHLGAFLAVTPNPGSAASGTEAAAATPSKVWGSSAGRIEPRGGGRGALPTSMGQHGPSARARAGRAPGPRPAREASPRLRVHKTFKFVVVGVLLQVVPSSAATIKLHDQSIGTQQWEHSPLGELCPPGSHRSEHPGACNRCTEGVGYTNASNNLFACLPCTACKSDEEERSPCTTTRNTACQCKPGTFRNDNSAEMCRKCSRGCPRGMVKVKDCTPWSDIECVHKESGNGHNIWVILVVTLVVPLLLVAVLIVCCCIGSGCGGDPKCMDRVCFWRLGLLRGPGAEDNAHNEILSNADSLSTFVSEQQMESQEPADLTGVTVQSPGEAQCLLGPAEAEGSQRRRLLVPANGADPTETLMLFFDKFANIVPFDSWDQLMRQLDLTKNEIDVVRAGTAGPGDALYAMLMKWVNKTGRNASIHTLLDALERMEERHAREKIQDLLVDSGKFIYLEDGTGSAVSLE tr10b_human Tumor necrosis factor receptor superfamily member 10B Death receptor 5 TNF-related apoptosis-inducing ligand receptor 2 UNQ160/PRO186 TNFRSF10B DR5 KILLER TRAILR2 TRICK2 ZTNFR9 protein protein human Homo sapiens Human MEQRGQNAPAASGARKRHGPGPREARGARPGPRVPKTLVLVVAAVLLLVSAESALITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKESGTKHSGEVPAVEETVTSSPGTPASPCSLSGIIIGVTVAAVVLIVAVFVCKSLLWKKVLPYLKGICSGGGGDPERVDRSSQRPGAEDNVLNEIVSILQPTQVPEQEMEVQEPAEPTGVNMLSPGESEHLLEPAEAERSQRRRLLVPANEGDPTETLRQCFDDFADLVPFDSWEPLMRKLGLMDNEIKVAKAEAAGHRDTLYTMLIKWVNKTGRDASVHTLLDALETLGERLAKQKIEDHLLSSGKFMYLEGNADSAMS tnfrsf10a-centd2-2 DR4 coimmunoprecipitates ARAP1-C Delta exon 30 in HEK293T lysates 708006 n/a 708010 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 708011 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O00220:p.[Phe368Pro;Asp369Leu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2 A, B Full-length ARAP1 Delta exon 30 co-immunoprecipitated with DR4, but less efficiently than its C-terminal part. In the DR4 death domain, phe368 asp369 are conserved when compared to DR5. Their substitution with the corresponding amino acids from TNFR1 (proline and leucine) in mutant DR4-FD/PL led to the severe suppression of ARAP1-CDexon30 co-immunoprecipitation with DR4. O00220; Q96P48-3 transfection tnfrsf10b-centd2 DR5 coimmunoprecipitates ARAP1-C Delta exon 30 in HEK293T lysates 708006 n/a 708010 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 708012 unspecified role unspecified role bait bait physical association physical association false false false 2 B Myc-tagged ARAP1-CDexon30 co-immunoprecipitated with DR5 but to a lesser extent than with DR4. tnfrsf10a-centd2 DR4 interacts with ARAP1-C Delta exon 30 in a yeast two-hybrid assay 708007 n/a 708010 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 708011 unspecified role unspecified role bait bait c-terminal intracellular region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 A The CENTD2 protein is an isoform lacking 11 amino acids near to the C-terminus but this could not be unambiguously mapped to a single UniprotKB isoform tnfrsf10a-centd2-1 DR4 (intracellar domain) interacts with ARAP1-C lacking exon3 in a directed yeast two-hybrid assay 708008 n/a 708010 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 708011 unspecified role unspecified role bait bait c-terminal intracellular region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 708033 708031 physical association physical association false false false 1 B centd2-tnfrsf10a DR4 and ARAP1 Delta exon 30 colocalize in NCTC cells 708009 n/a 708010 unspecified role unspecified role neutral component neutral component N/A 708011 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 A Upon TRAIL treatment, both ARAP1 and DR4 co-localized in early endosomes