IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ itahana-2007-1 Targeted Inactivation of Mdm2 RING Finger E3 Ubiquitin Ligase Activity in the Mouse Reveals Mechanistic Insights into p53 Regulation. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Itahana K., Mao H., Jin A., Itahana Y., Clegg HV., Lindstrom MS., Bhat KP., Godfrey VL., Evan GI., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis ypzhang@med.unc.edu Cancer Cell (1535-6108) 2007 Only protein-protein interactions 6 imex curation Cancer - Interactions investigated in the context of cancer itahana-2007-2 Targeted Inactivation of Mdm2 RING Finger E3 Ubiquitin Ligase Activity in the Mouse Reveals Mechanistic Insights into p53 Regulation. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip weight identificat confirmation by molecular weight weight identificat Itahana K., Mao H., Jin A., Itahana Y., Clegg HV., Lindstrom MS., Bhat KP., Godfrey VL., Evan GI., Zhang Y. Apoptosis - Interactions involving proteins with a function related to apoptosis ypzhang@med.unc.edu Cancer Cell (1535-6108) 2007 Only protein-protein interactions 6 imex curation Cancer - Interactions investigated in the context of cancer mdm2_mouse E3 ubiquitin-protein ligase Mdm2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein Mdm2 RING-type E3 ubiquitin transferase Mdm2 protein protein mouse Mus musculus Mouse MCNTNMSVSTEGAASTSQIPASEQETLVRPKPLLLKLLKSVGAQNDTYTMKEIIFYIGQYIMTKRLYDEKQQHIVYCSNDLLGDVFGVPSFSVKEHRKIYAMIYRNLVAVSQQDSGTSLSESRRQPEGGSDLKDPLQAPPEEKPSSSDLISRLSTSSRRRSISETEENTDELPGERHRKRRRSLSFDPSLGLCELREMCSGGSSSSSSSSSESTETPSHQDLDDGVSEHSGDCLDQDSVSDQFSVEFEVESLDSEDYSLSDEGHELSDEDDEVYRVTVYQTGESDTDSFEGDPEISLADYWKCTSCNEMNPPLPSHCKRCWTLRENWLPDDKGKDKVEISEKAKLENSAQAEEGLDVPDGKKLTENDAKEPCAEEDSEEKAEQTPLSQESDDYSQPSTSSSIVYSSQESVKELKEETQDKDESVESSFSLNAIEPCVICQGRPKNGCIVHGKTGHLMSCFTCAKKLKKRNKPCPVCRQPIQMIVLTYFN p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD rl5_mouse 60S ribosomal protein L5 Rpl5 protein protein mouse Mus musculus Mouse MGFVKVVKNKAYFKRYQVRFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVNGGEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNNVTPDMMEEMYKKAHAAIRENPVYEKKPKREVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES rl11_mouse 60S ribosomal protein L11 Rpl11 protein protein mouse Mus musculus Mouse MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKYDGIILPGK ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD mdm2-tp53 Mdm2 coimmunoprecipitates p53 in MEF lysates 673721 N/A 673723 unspecified role unspecified role bait bait N/A 673724 unspecified role unspecified role prey prey region modified ligand-binding domain of the mouse estrogen receptor tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3 B The p53 protein was tagged witha modified ligand-binding domain of the mouse estrogen receptor (ERTAM), which binds 4-hydroxytamoxifen but not endogenous estrogen. Mdm2 C462A has similar p53-binding activity to wild-type Mdm2. mdm2-rpl5-1 Mdm2 coimmunoprecipitates ribosomal protein L5 in MEF lysates 673721 N/A 673723 unspecified role unspecified role bait bait N/A 673725 unspecified role unspecified role prey prey physical association physical association false false false 3 B Mdm2 C462A has similar p53-binding activity to wild-type Mdm2. mdm2-rpl11-2 Mdm2 coimmunoprecipitates ribosomal protein L11 in MEF lysates 673721 N/A 673723 unspecified role unspecified role bait bait N/A 673726 unspecified role unspecified role prey prey physical association physical association false false false 3 B Mdm2 C462A has similar p53-binding activity to wild-type Mdm2. mdm2-tp53-1 Mdm2 coimmunoprecipitates with tsp53 in MEF lysates 673721 N/A 673724 unspecified role unspecified role bait bait ala135val biological feature biological feature certain certain sequence position certain certain certain sequence position certain false N/A 673723 unspecified role unspecified role prey prey physical association physical association false false false 3 C Mdm2 C462A has similar p53-binding activity to wild-type Mdm2. A temperature sensitive mutant p53 (A135V) was used. P02340 infection mdm2-rps27a Polyubiquitination of Mdm2 in MEFs is enhanced by expression of p53 673722 N/A 673723 unspecified role unspecified role bait bait N/A 673727 unspecified role unspecified role prey prey physical association physical association false false false 3 B p53 - Infection with adenoviruses expressing human p53 enhanced polyubiquitination of Mdm2. tp53-rps27a Polyubiquitination of p53 in MEFs is promoted by wild-type Mdm2 673722 N/A 673728 unspecified role unspecified role bait bait N/A 673727 unspecified role unspecified role prey prey physical association physical association false false false 3 C Mdm2 - Wild-type Mdm2 and not Mdm2(C462A) promotes polyubiquitination of p53. P04637 infection