IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gordan-2007-1 HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation gordan-2007-2 HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Rejected 2007-MAY-25 by DAVET. I think the same comments made in gordan-2007-1 are relevant here also. Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation gordan-2007-3 HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation gordan-2007-4 HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation gordan-2007-5 HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation gordan-2007-6 HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. human-wt8 Homo sapiens WT8 cells (786-O renal carcinoma cells expressing pVHL) wt8 WT8 cells are 786-O renal carcinoma cells expressing pVHL WT8 cells are 786-O renal carcinoma cells expressing pVHL. CRL-1932 is the ATCC identifier for 786-O cells. CRL-1932 is the ATCC identifier for 786-O cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gordan JD., Bertout JA., Hu CJ., Diehl JA., Simon MC. Cancer - Interactions investigated in the context of cancer Cells were maintained under hypoxic conditions (0.5% O2, 5% CO2, 94.5% N2) for varying amounts of time, then cells were lysed and IP assays performed. celeste2@mail.med.upenn.edu Cancer Cell (1535-6108) 2007 Only protein-protein interactions 20 imex curation cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL cdn1a_human Cyclin-dependent kinase inhibitor 1 CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 p21 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 protein protein human Homo sapiens Human MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT pias2_human E3 SUMO-protein ligase PIAS2 Protein inhibitor of activated STAT x Msx-interacting zinc finger protein DAB2-interacting protein Androgen receptor-interacting protein 3 PIAS-NY protein PIAS2 Protein inhibitor of activated STAT2 PIASX E3 SUMO-protein transferase PIAS2 protein protein human Homo sapiens Human MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLEGLSDLSTIKSSVFSLDGGSSPVEPDLAVAGIHSLPSTSVTPHSPSSPVGSVLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPCSVTVASEASKKKVDVIDLTIESSSDEEEDPPAKRKCIFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPISSMSSDLPGLDFLSLIPVDPQYCPPMFLDSLTSPLTASSTSVTTTSSHESSTHVSSSSSRSETGVITSSGSNIPDIISLD myc_human Myc proto-oncogene protein BHLHE39 Transcription factor p64 MYC Proto-oncogene c-Myc Class E basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA sp1_human Transcription factor Sp1 SP1 TSFP1 protein protein human Homo sapiens Human MSDQDHSMDEMTAVVKIEKGVGGNNGGNGNGGGAFSQARSSSTGSSSSTGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGSNGSESSKNRTVSGGQYVVAAAPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTTISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSSGTNSQGQTPQRVSGLQGSDALNIQQNQTSGGSLQAGQQKEGEQNQQTQQQQILIQPQLVQGGQALQALQAAPLSGQTFTTQAISQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCTWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF max_human Protein max MAX BHLHD4 Class D basic helix-loop-helix protein 4 Myc-associated factor X protein protein human Homo sapiens Human MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS mad1_human Max dimerization protein 1 MAD Protein MAD MXD1 protein protein human Homo sapiens Human MAAAVRMNIQMLLEAADYLERREREAEHGYASMLPYNNKDRDALKRRNKSKKNNSSSRSTHNEMEKNRRAHLRLCLEKLKGLVPLGPESSRHTTLSLLTKAKLHIKKLEDCDRKAVHQIDQLQREQRHLKRQLEKLGIERIRMDSIGSTVSSERSDSDREEIDVDVESTDYLTGDLDWSSSSVSDSDERGSMQSLGSDEGYSSTSIKRIKLQDSHKACLGL hif1a_human Hypoxia-inducible factor 1-alpha ARNT-interacting protein Member of PAS protein 1 Basic-helix-loop-helix-PAS protein MOP1 HIF1A MOP1 BHLHE78 PASD8 PAS domain-containing protein 8 Class E basic helix-loop-helix protein 78 protein protein human Homo sapiens Human MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN epas1_human Endothelial PAS domain-containing protein 1 EPAS1 HIF2A MOP2 Member of PAS protein 2 Basic-helix-loop-helix-PAS protein MOP2 BHLHE73 PASD2 Hypoxia-inducible factor 2-alpha HIF-1-alpha-like factor PAS domain-containing protein 2 Class E basic helix-loop-helix protein 73 protein protein human Homo sapiens Human MTADKEKKRSSSERRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVYNNCPPHNSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSGKGAVSEKSNFLFTKLKEEPEELAQLAPTPGDAIISLDFGNQNFEESSAYGKAILPPSQPWATELRSHSTQSEAGSLPAFTVPQAAAPGSTTPSATSSSSSCSTPNSPEDYYTSLDNDLKIEVIEKLFAMDTEAKDQCSTQTDFNELDLETLAPYIPMDGEDFQLSPICPEERLLAENPQSTPQHCFSAMTNIFQPLAPVAPHSPFLLDKFQQQLESKKTEPEHRPMSSIFFDAGSKASLPPCCGQASTPLSSMGGRSNTQWPPDPPLHFGPTKWAVGDQRTEFLGAAPLGPPVSPPHVSTFKTRSAKGFGARGPDVLSPAMVALSNKLKLKRQLEYEEQAFQDLSGGDPPGGSTSHLMWKRMKNLRGGSCPLMPDKPLSANVPNDKFTQNPMRGLGHPLRHLPLPQPPSAISPGENSKSRFPPQCYATQYQDYSLSSAHKVSGMASRLLGPSFESYLLPELTRYDCEVNVPVLGSSTLLQGGDLLRALDQAT cdk2-cdkn1a CDK2 coimmunoprecipitated p21 in HCT116 cell lysates 605114 N/A 605120 unspecified role unspecified role bait bait N/A 605121 unspecified role unspecified role prey prey physical association physical association false false false 3 E Hypoxia (0.5% O2 for 48 h) substantially increased levels of p21 interacting with Cdk2. cdk2-cdkn1b CDK2 coimmunoprecipitated p27 in HCT116 cell lysates 605114 N/A 605120 unspecified role unspecified role bait bait N/A 605122 unspecified role unspecified role prey prey physical association physical association false false false 3 E Hypoxia (0.5% O2 for 48 h) increased levels of p27 interacting with Cdk2 but not to the same extent as p21. However, it should be noted that HCT116 cells express higher levels of p21 than p27. myc-max-2 c-Myc coimmunoprecipitates Sp1, Miz1 and Max in HCT116 cell lysates 605114 N/A 605123 unspecified role unspecified role prey prey N/A 605124 unspecified role unspecified role bait bait N/A 605125 unspecified role unspecified role prey prey n/a 605126 unspecified role unspecified role prey prey association association false false false 6 A Hypoxic conditions (0.5% O2 for 4 h and 20 h) correlated with a substantial decrease in the amount of Sp1, Miz1 and Max coimmunoprecipitated with c-Myc. mad1l1-max Max coimmunoprecipitates with Mad1 in HCT116 cell lysates 605114 n/a 605126 unspecified role unspecified role prey prey N/A 605127 unspecified role unspecified role bait bait physical association physical association false false false 6 A This interaction increased under hypoxic conditions (0.5% O2 for 4 h and 20 h). sp1-myc c-Myc coimmunoprecipitates with Sp1 in HCT116 cell lysates 605114 N/A 605125 unspecified role unspecified role bait bait N/A 605124 unspecified role unspecified role prey prey physical association physical association false false false 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with a substantial decrease in the amount of Myc coimmunoprecipitated with Sp1. pias2-myc Miz1 coimmunoprecipitates c-Myc in HCT116 cell lysates 605114 N/A 605124 unspecified role unspecified role prey prey N/A 605123 unspecified role unspecified role bait bait physical association physical association false false false 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with a substantial decrease in the amount of c-Myc coimmunoprecipitated with Miz1. max-myc-5 c-Myc coimmunoprecipitates with Max in HCT116 cell lysates 605114 N/A 605124 unspecified role unspecified role prey prey n/a 605126 unspecified role unspecified role bait bait physical association physical association false false false 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with a substantial decrease in the amount of c-Myc coimmunoprecipitated with Max. cdk2-cdkn1b-1 CDK2 coimmunoprecipitated p27 in WT8 cell lysates 605115 N/A 605122 unspecified role unspecified role prey prey N/A 605120 unspecified role unspecified role bait bait physical association physical association false false false 3 E Hypoxia (0.5% O2 for 48 h) almost totally abrogated the interaction between p27 and Cdk2, in contrast to the effect on the interaction in HCT116 cells. mad1l1-max-1 Max coimmunoprecipitates with Mad1 in WT8 cell lysates 605115 n/a 605126 unspecified role unspecified role prey prey N/A 605127 unspecified role unspecified role bait bait physical association physical association false false false 6 A This interaction decreased slightly under hypoxic conditions (0.5% O2 for 4 h and 20 h), in contrast to the situation in HCT116 cells. myc-max-3 c-Myc coimmunoprecipitates Sp1, Miz1 and Max in WT8 cell lysates 605115 N/A 605124 unspecified role unspecified role bait bait N/A 605123 unspecified role unspecified role prey prey N/A 605125 unspecified role unspecified role prey prey n/a 605126 unspecified role unspecified role prey prey association association false false false 6 A Hypoxic conditions (0.5% O2 for 4 h and 20 h) correlated with an increase in the amount of Sp1, Miz1 and Max coimmunoprecipitated with c-Myc, although not as marked as the decrease seen in HCT116 cells. sp1-myc-1 c-Myc coimmunoprecipitates with Sp1 in WT8 cell lysates 605115 N/A 605125 unspecified role unspecified role bait bait N/A 605124 unspecified role unspecified role prey prey physical association physical association false false false 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with an increase in the amount of Myc coimmunoprecipitated with Sp1, in contrast to the situation in HCT116 cells. pias2-myc-1 Miz1 coimmunoprecipitates c-Myc in WT8 cell lysates 605115 N/A 605123 unspecified role unspecified role bait bait N/A 605124 unspecified role unspecified role prey prey physical association physical association false false false 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with an increase in the amount of c-Myc coimmunoprecipitated with Miz1, in contrast to the situation in HCT116 cells. max-myc-6 c-Myc coimmunoprecipitates with Max in WT8 cell lysates 605115 N/A 605124 unspecified role unspecified role prey prey n/a 605126 unspecified role unspecified role bait bait physical association physical association false false false 6 B Hypoxic conditions (0.5% O2 for 20 h) correlated with an increase in the amount of c-Myc coimmunoprecipitated with Max, in contrast to the situation in HCT116. max-hif1a Max coimmunoprecipitates HIF-1 alpha in WT8 lysates 605116 N/A 605128 unspecified role unspecified role prey prey n/a 605126 unspecified role unspecified role bait bait physical association physical association false false false 6 D Q16665 over-expressed Increasing levels of expression of HIF-1 alpha and HIF-1 alpha/Max binding correlated with decreasing levels of c-Myc/Max and HIF-2 alpha/Max binding. max-epas1 Max coimmunoprecipitates HIF-2 alpha in WT8 lysates 605116 n/a 605126 unspecified role unspecified role bait bait N/A 605129 unspecified role unspecified role prey prey physical association physical association false false false 6 D Increasing levels of expression of HIF-1 alpha and HIF-1 alpha/Max binding correlated with decreasing levels of c-Myc/Max and HIF-2 alpha/Max binding. HIF-2 alpha expression decreases with increasing HIF-1 alpha expression but the decrease in HIF-2 alpha/Max binding is greater than the decrease in expression. Increasing levels of HIF-1 alpha expression correlate with decreasing levels of the HIF-2 alpha/Max onteraction. max-myc-7 Max coimmunoprecipitates c-Myc in WT8 lysates 605116 n/a 605126 unspecified role unspecified role bait bait N/A 605124 unspecified role unspecified role prey prey physical association physical association false false false 6 D Increasing levels of expression of HIF-1 alpha and HIF-1 alpha/Max binding correlated with decreasing levels of c-Myc/Max and HIF-2 alpha/Max binding. Increasing levels of HIF-1 alpha expression correlate with decreasing levels of the c-Myc/Max interaction. max-epas1-1 Max coimmunoprecipitates HIF-2 alpha in WT8 lysates 605117 n/a 605126 unspecified role unspecified role bait bait N/A 605129 unspecified role unspecified role prey prey physical association physical association false false false 6 E Q99814 over-expressed This interaction occurred under hypoxic conditions. max-myc-8 Max coimmunoprecipitates c-Myc in WT8 lysates 605117 N/A 605124 unspecified role unspecified role prey prey n/a 605126 unspecified role unspecified role bait bait physical association physical association false false false 6 E This interaction occurred under hypoxic conditions. Increasing c-Myc/Max binding correlated with increasing levels of expression of HIF-2 alpha. max-myc-9 Max coimmunoprecipitates c-Myc in WT8 lysates 605118 n/a 605126 unspecified role unspecified role bait bait N/A 605124 unspecified role unspecified role prey prey physical association physical association false false false S6 B There was no difference in c-Myc/Max binding under normoxia correlating with HIF-1 alpha overexpression, in contrast to the findings under hypoxic conditions. max-myc-10 Max coimmunoprecipitates c-Myc in WT8 lysates 605119 n/a 605126 unspecified role unspecified role bait bait N/A 605124 unspecified role unspecified role prey prey physical association physical association false false false S6 C There was no difference in c-Myc/Max binding under normoxia correlating with HIF-2 alpha overexpression, in contrast to the findings under hypoxic conditions.