IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sun-2007-1 PRAK is essential for ras-induced senescence and tumor suppression. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Sun P., Yoshizuka N., New L., Moser BA., Li Y., Liao R., Xie C., Chen J., Deng Q., Yamout M., Dong MQ., Frangou CG., Yates JR., Wright PE., Han J. Apoptosis - Interactions involving proteins with a function related to apoptosis Note that the cell type expressed in was searched for in the cited references where necessary. pqsun@scripps.edu,jhan@scripps.edu Cell (0092-8674) 2007 Only protein-protein interactions 7 imex curation Cancer - Interactions investigated in the context of cancer p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD mapk5_human MAP kinase-activated protein kinase 5 MAPKAPK5 PRAK p38-regulated/activated protein kinase protein protein human Homo sapiens Human MSEESDMDKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEEQTTSHESQ mk11_human Mitogen-activated protein kinase 11 MAPK11 PRKM11 SAPK2 Mitogen-activated protein kinase p38 beta p38-2 Stress-activated protein kinase 2b SAPK2B protein protein human Homo sapiens Human MSGPRAGFYRQELNKTVWEVPQRLQGLRPVGSGAYGSVCSAYDARLRQKVAVKKLSRPFQSLIHARRTYRELRLLKHLKHENVIGLLDVFTPATSIEDFSEVYLVTTLMGADLNNIVKCQALSDEHVQFLVYQLLRGLKYIHSAGIIHRDLKPSNVAVNEDCELRILDFGLARQADEEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLQGKALFPGSDYIDQLKRIMEVVGTPSPEVLAKISSEHARTYIQSLPPMPQKDLSSIFRGANPLAIDLLGRMLVLDSDQRVSAAEALAHAYFSQYHDPEDEPEAEPYDESVEAKERTLEEWKELTYQEVLSFKPPEPPKPPGSLEIEQ p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD mapk5_mouse MAP kinase-activated protein kinase 5 Mapkapk5 protein protein mouse Mus musculus Mouse MSEDSDMEKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALQHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKVDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRKKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDGIYIHDHENGTEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDALQSFSWNGRGFTDKVDRLKLAEVVKQVIEEQTLPHEPQ hspb1_human Heat shock protein beta-1 HSPB1 HSP27 HSP28 Heat shock 27 kDa protein Stress-responsive protein 27 Estrogen-regulated 24 kDa protein 28 kDa heat shock protein protein protein human Homo sapiens Human MTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK 1B4DC44A6F6606D5 atf2_human Cyclic AMP-dependent transcription factor ATF-2 ATF2 CREB2 CREBP1 Activating transcription factor 2 cAMP response element-binding protein CRE-BP1 HB16 Cyclic AMP-responsive element-binding protein 2 protein protein human Homo sapiens Human MKFKLHVNSARQYKDLWNMSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVADQTPTPTRFLKNCEEVGLFNELASPFENEFKKASEDDIKKMPLDLSPLATPIIRSKIEEPSVVETTHQDSPLPHPESTTSDEKEVPLAQTAQPTSAIVRPASLQVPNVLLTSSDSSVIIQQAVPSPTSSTVITQAPSSNRPIVPVPGPFPLLLHLPNGQTMPVAIPASITSSNVHVPAAVPLVRPVTMVPSVPGIPGPSSPQPVQSEAKMRLKAALTQQHPPVTNGDTVKGHGSGLVRTQSEESRPQSLQQPATSTTETPASPAHTTPQTQSTSGRRRRAANEDPDEKRRKFLERNRAAASRCRQKRKVWVQSLEKKAEDLSSLNGQLQSEVTLLRNEVAQLKQLLLAHKDCPVTAMQKKSGYHTADKDDSSEDISVPSSPHTEAIQHSSVSTSNGVSSTSKAEAVATSVLTQMADQSTEPALSQIVMAPSSQSQPSGS mapkapk5-tp53 Human HA-PRAK phosphorylates human p53 in vitro 593034 N/A 593035 enzyme target enzyme target substrate substrate unspecified role unspecified role n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P04637:p.Ser37Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 593036 enzyme enzyme unspecified role unspecified role region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8IW41:p.Thr182Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8IW41:p.Lys51Met mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. phosphorylation phosphorylation reaction phosphorylation false false false 7 A, B, C, D HA-PRAK immunoprecipitated from BJ cells transduced with Ha-RasV12, MKK3E or MKK6E had higher levels of kinase activity than when the BJ cells were only transduced with vector. P04637 Ser37 is phosphorylated by PRAK. Phosphopeptide mapping by mass spectrometry confirmed Ser37 was the only residue phosphorylated by PRAK. The BJ cells from which PRAK was immunoprecipitated were senescent. mapkapk5-tp53-1 Human His-PRAK phosphorylates human p53 in vitro 593034 N/A 593035 enzyme target enzyme target substrate substrate unspecified role unspecified role P04637:p.Ser37Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 593036 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation false false false 7 E P04637 Ser37 is phosphorylated by PRAK. Phosphopeptide mapping by mass spectrometry confirmed Ser37 was the only residue phosphorylated by PRAK. mapk11-tp53 Human His-p38beta phosphorylates human p53 in vitro 593034 n/a 593037 enzyme enzyme unspecified role unspecified role N/A 593035 enzyme target enzyme target substrate substrate unspecified role unspecified role n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P04637:p.Ser33Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false S10 B HA-PRAK immunoprecipitated from BJ cells transduced with Ha-RasV12, MKK3E or MKK6E had higher levels of kinase activity than when the BJ cells were only transduced with vector. P04637 Ser33 is phosphorylated by p38. ENZYME protein AF001008 mapkapk5-tp53-2 PRAK immunoprecipitated from mouse normal skin or papilloma phosphorylated p53 593034 N/A 593038 enzyme target enzyme target substrate substrate unspecified role unspecified role P02340:p.Ser37Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false 4-60 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false The range was 1-57 but UniProt updated sequence so now range is 4-60. ecoli Escherichia coli (strain K12) N/A 593039 enzyme enzyme unspecified role unspecified role mouse-skin Mus musculus skin cells skin phosphorylation phosphorylation reaction phosphorylation false false false 7 I The PRAK immunoprecipitated from wild-type papilloma had increased kinase activity as compared to PRAK from adjacent normal skin. This suggests p53 Ser37 is phosphorylated by activated PRAK in tumours with senescent features. mapk5-hspb1 PRAK immunoprecipitated from mouse normal skin or papilloma phosphorylated HSP27 593034 N/A 593040 enzyme target enzyme target substrate substrate unspecified role unspecified role ecobb Escherichia coli (strain B / BL21) N/A 593039 enzyme enzyme unspecified role unspecified role mouse-skin Mus musculus skin cells skin phosphorylation phosphorylation reaction phosphorylation false false false 5 C Phosphorylation of HSPB1 PRAK from papilloma had increased kinase activity as compared with PRAK isolated from normal skin in the same mouse. mapk11-atf2 Human p38 isolated from BJ cells phosphorylates ATF2 in vitro 593034 N/A 593037 enzyme enzyme unspecified role unspecified role human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. N/A 593041 enzyme target enzyme target substrate substrate unspecified role unspecified role ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation false false false S10 A Phosphorylation of ATF2 Transduction of BJ cells with Ha-RasV12 increased p38 kinase activity as compared to p38 isolated from cells transduced with the vector control. mapk11-tp53-1 Human p38 isolated from BJ cells phosphorylates p53 in vitro 593034 N/A 593035 enzyme target enzyme target substrate substrate unspecified role unspecified role n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P04637:p.Ser33Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false N/A 593037 enzyme enzyme unspecified role unspecified role human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. phosphorylation phosphorylation reaction phosphorylation false false false S10 A Phosphorylation of TP53 Transduction of BJ cells with Ha-RasV12 increased p38 kinase activity as compared to p38 isolated from cells transduced with the vector control. The S37D protein was accessible to kinases since it was readily phosphorylated by recombinant and immunoprecipitated p38 at Ser33.