IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cho-2006-1 Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org A human testis cDNA library was used. Cancer Immun. 2006 cho-2006-2 Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). human-sk_mel_37 Homo sapiens melanoma derived cell line SK-MEL-37 sk_mel_37 Human melanoma derived SK-MEL-37 cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org A human testis cDNA library was used. Cancer Immun. 2006 cho-2006-3 Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). human-u266 Homo sapiens myeloma cell line U-266 u266 Human myeloma cell line U-266 confirmed as human with IEF of AST, MDH, NP established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968; cells were described to produce IgE lambda; cells express mRNA for BCL2 gene anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org A human testis cDNA library was used. Cancer Immun. 2006 cho-2006-4 Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). human-sk_mel_37 Homo sapiens melanoma derived cell line SK-MEL-37 sk_mel_37 Human melanoma derived SK-MEL-37 cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org A human testis cDNA library was used. Cancer Immun. 2006 ctg1b_human Cancer/testis antigen 1 CTAG1A LAGE2A LAGE2B L antigen family member 2 Autoimmunogenic cancer/testis antigen NY-ESO-1 Cancer/testis antigen 6.1 CTAG1B CTAG CTAG1 ESO1 LAGE2 protein protein human Homo sapiens Human MQAEGRGTGGSTGDADGPGGPGIPDGPGGNAGGPGEAGATGGRGPRGAGAARASGPGGGAPRGPHGGAASGLNGCCRCGARGPESRLLEFYLAMPFATPMEAELARRSLAQDAPPLPVPGVLLKEFTVSGNILTIRLTAADHRQLQLSISSCLQQLSLLMWITQCFLPVFLAQPPSGQRR magc1_human Melanoma-associated antigen C1 MAGE-C1 antigen Cancer/testis antigen 7.1 MAGEC1 protein protein human Homo sapiens Human MGDKDMPTAGMPSLLQSSSESPQSCPEGEDSQSPLQIPQSSPESDDTLYPLQSPQSRSEGEDSSDPLQRPPEGKDSQSPLQIPQSSPEGDDTQSPLQNSQSSPEGKDSLSPLEISQSPPEGEDVQSPLQNPASSFFSSALLSIFQSSPESTQSPFEGFPQSVLQIPVSAASSSTLVSIFQSSPESTQSPFEGFPQSPLQIPVSRSFSSTLLSIFQSSPERTQSTFEGFAQSPLQIPVSPSSSSTLLSLFQSFSERTQSTFEGFAQSSLQIPVSPSFSSTLVSLFQSSPERTQSTFEGFPQSPLQIPVSSSSSSTLLSLFQSSPERTHSTFEGFPQSLLQIPMTSSFSSTLLSIFQSSPESAQSTFEGFPQSPLQIPGSPSFSSTLLSLFQSSPERTHSTFEGFPQSPLQIPMTSSFSSTLLSILQSSPESAQSAFEGFPQSPLQIPVSSSFSYTLLSLFQSSPERTHSTFEGFPQSPLQIPVSSSSSSSTLLSLFQSSPECTQSTFEGFPQSPLQIPQSPPEGENTHSPLQIVPSLPEWEDSLSPHYFPQSPPQGEDSLSPHYFPQSPPQGEDSLSPHYFPQSPQGEDSLSPHYFPQSPPQGEDSMSPLYFPQSPLQGEEFQSSLQSPVSICSSSTPSSLPQSFPESSQSPPEGPVQSPLHSPQSPPEGMHSQSPLQSPESAPEGEDSLSPLQIPQSPLEGEDSLSSLHFPQSPPEWEDSLSPLHFPQFPPQGEDFQSSLQSPVSICSSSTSLSLPQSFPESPQSPPEGPAQSPLQRPVSSFFSYTLASLLQSSHESPQSPPEGPAQSPLQSPVSSFPSSTSSSLSQSSPVSSFPSSTSSSLSKSSPESPLQSPVISFSSSTSLSPFSEESSSPVDEYTSSSDTLLESDSLTDSESLIESEPLFTYTLDEKVDELARFLLLKYQVKQPITKAEMLTNVISRYTGYFPVIFRKAREFIEILFGISLREVDPDDSYVFVNTLDLTSEGCLSDEQGMSQNRLLILILSIIFIKGTYASEEVIWDVLSGIGVRAGREHFAFGEPRELLTKVWVQEHYLEYREVPNSSPPRYEFLWGPRAHSEVIKRKVVEFLAMLKNTVPITFPSSYKDALKDVEERAQAIIDTTDDSTATESASSSVMSPSFSSE rusc2_human AP-4 complex accessory subunit RUSC2 Interacting protein of Rab1 RUN and SH3 domain-containing protein 2 RUSC2 KIAA0375 protein protein human Homo sapiens Human MDSPPKLTGETLIVHHIPLVHCQVPDRQCCGGAGGGGGSTRPNPFCPPELGITQPDQDLGQADSLLFSSLHSTPGGTARSIDSTKSRSRDGRGPGAPKRHNPFLLQEGVGEPGLGDLYDDSIGDSATQQSFHLHGTGQPNFHLSSFQLPPSGPRVGRPWGTTRSRAGVVEGQEQEPVMTLDTQQCGTSHCCRPELEAETMELDECGGPGGSGSGGGASDTSGFSFDQEWKLSSDESPRNPGCSGSGDQHCRCSSTSSQSEAADQSMGYVSDSSCNSSDGVLVTFSTLYNKMHGTPRANLNSAPQSCSDSSFCSHSDPGAFYLDLQPSPFESKMSYESHHPESGGREGGYGCPHASSPELDANCNSYRPHCEPCPAVADLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEEHTKISPPPGPGPDPGPSQPSEYYLFQKPEVQPEEQEAVSSSTQAAAAVGPTVLEGQVYTNTSPPNLSTGRQRSRSYDRSLQRSPPVRLGSLERMLSCPVRLSEGPAAMAGPGSPPRRVTSFAELAKGRKKTGGSGSPPLRVSVGDSSQEFSPIQEAQQDRGAPLDEGTCCSHSLPPMPLGPGMDLLGPDPSPPWSTQVCQGPHSSEMPPAGLRATGQGPLAQLMDPGPALPGSPANSHTQRDARARADGGGTESRPVLRYSKEQRPTTLPIQPFVFQHHFPKQLAKARALHSLSQLYSLSGCSRTQQPAPLAAPAAQVSVPAPSGEPQASTPRATGRGARKAGSEPETSRPSPLGSYSPIRSVGPFGPSTDSSASTSCSPPPEQPTATESLPPWSHSCPSAVRPATSQQPQKEDQKILTLTEYRLHGTGSLPPLGSWRSGLSRAESLARGGGEGSMATRPSNANHLSPQALKWREYRRKNPLGPPGLSGSLDRRSQEARLARRNPIFEFPGSLSAASHLNCRLNGQAVKPLPLTCPDFQDPFSLTEKPPAEFCLSPDGSSEAISIDLLQKKGLVKAVNIAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLVLKYLCPAVRAVLEDGLKAFVLDVIIGQRKNMPWSVVEASTQLGPSTKVLHGLYNKVSQFPELTSHTMRFNAFILGLLNIRSLEFWFNHLYNHEDIIQTHYQPWGFLSAAHTVCPGLFEELLLLLQPLALLPFSLDLLFQHRLLQSGQQQRQHKELLRVSQDLLLSAHSTLQLARARGQEGPGDVDRAAQGERVKGVGASEGGEEEEEEEETEEVAEAAGGSGRARWARGGQAGWWYQLMQSSQVYIDGSIEGSRFPRGSSNSSSEKKKGAGGGGPPQAPPPREGVVEGAEACPASEEALGRERGWPFWMGSPPDSVLAELRRSREREGPAASPAENEEGASEPSPGGIKWGHLFGSRKAQREARPTNRLPSDWLSLDKSMFQLVAQTVGSRREPEPKESLQEPHSPALPSSPPCEVQALCHHLATGPGQLSFHKGDILRVLGRAGGDWLRCSRGPDSGLVPLAYVTLTPTPSPTPGSSQN t2fa_human General transcription factor IIF subunit 1 Transcription initiation factor IIF subunit alpha GTF2F1 RAP74 Transcription initiation factor RAP74 General transcription factor IIF 74 kDa subunit protein protein human Homo sapiens Human MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRGRRKASELRIHDLEDDLEMSSDASDASGEEGGRVPKAKKKAPLAKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSSSQEEPESKAKAPQQEEGPKGVDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEESDIDSEASSALFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAAKRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTKKTGLSSEQTVNVLAQILKRLNPERKMINDKMHFSLKE echd2_human Enoyl-CoA hydratase domain-containing protein 2, mitochondrial ECHDC2 protein protein human Homo sapiens Human MLRVLCLLRPWRPLRARGCASDGAAGGSEIQVRALAGPDQGITEILMNRPSARNALGNVFVSELLETLAQLREDRQVRVLLFRSGVKGVFCAGADLKEREQMSEAEVGVFVQRLRGLMNDIAAFPAPTIAAMDGFALGGGLELALACDLRVAASSAVMGLIETTRGLLPGAGGTQRLPRCLGVALAKELIFTGRRLSGTEAHVLGLVNHAVAQNEEGDAAYQRARALAQEILPQAPIAVRLGKVAIDRGTEVDIASGMAIEGMCYAQNIPTRDRLEGMAAFREKRTPKFVGK atp5l_human ATP synthase subunit g, mitochondrial ATP5MG ATP synthase membrane subunit g ATP5L protein protein human Homo sapiens Human MAQFVRNLVEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQSLKKIVNSAQTGSFKQLTVKEAVLNGLVATEVLMWFYVGEIIGKRGIIGYDV rm19_human 39S ribosomal protein L19, mitochondrial MRPL15 39S ribosomal protein L15, mitochondrial MRPL19 KIAA0104 Mitochondrial large ribosomal subunit protein bL19m protein protein human Homo sapiens Human MAACIAAGHWAAMGLGRSFQAARTLLPPPASIACRVHAGPVRQQSTGPSEPGAFQPPPKPVIVDKHRPVEPERRFLSPEFIPRRGRTDPLKFQIERKDMLERRKVLHIPEFYVGSILRVTTADPYASGKISQFLGICIQRSGRGLGATFILRNVIEGQGVEICFELYNPRVQEIQVVKLEKRLDDSLLYLRDALPEYSTFDVNMKPVVQEPNQKVPVNELKVKMKPKPWSKRWERPNFNIKGIRFDLCLTEQQMKEAQKWNQPWLEFDMMREYDTSKIEAAIWKEIEASKRS h2az_human Histone H2A.Z H2AZ1 H2AFZ H2AZ protein protein human Homo sapiens Human MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTV magec1-ctag1b In a yeast two-hybrid assay MAGE-C1/CT7 interacts with NY-ESO-1 588005 N/A 588009 unspecified role unspecified role prey prey N/A 588010 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Table 1 magec1-rusc2 In a yeast two-hybrid assay MAGE-C1/CT7 interacts with RUSC2 588005 N/A 588010 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 588011 unspecified role unspecified role prey prey physical association physical association false false false Table 1 magec1-gtf2f1 In a yeast two-hybrid assay MAGE-C1/CT7 interacts with GTF2F1 588005 N/A 588012 unspecified role unspecified role prey prey N/A 588010 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Table 1 magec1-echdc2 In a yeast two-hybrid assay MAGE-C1/CT7 interacts with ECHDC2 588005 N/A 588010 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 588013 unspecified role unspecified role prey prey physical association physical association false false false Table 1 magec1-atp5l In a yeast two-hybrid assay MAGE-C1/CT7 interacts with ATP synthase subunit g (mitochondrial) 588005 N/A 588014 unspecified role unspecified role prey prey N/A 588010 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Table 1 magec1-mrpl19 In a yeast two-hybrid assay MAGE-C1/CT7 interacts with mitochondrial ribosomal protein L19 588005 N/A 588010 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 588015 unspecified role unspecified role prey prey physical association physical association false false false Table 1 magec1-h2afz In a yeast two-hybrid assay MAGE-C1/CT7 interacts with Histone H2AZ 588005 N/A 588016 unspecified role unspecified role prey prey N/A 588010 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false Table 1 magec1-ctag1b-1 MAGE-C1/CT7 coimmunoprecipitates NY-ESO-1 588006 N/A 588010 unspecified role unspecified role bait bait N/A 588009 unspecified role unspecified role prey prey physical association physical association false false false 1 A The band corresponding to NY-ESO-1 is not visible in the picture of the gel. ctag1b-magec1 MAGE-C1/CT7 coimmunoprecipitates with NY-ESO-1 588006 N/A 588009 unspecified role unspecified role bait bait N/A 588010 unspecified role unspecified role prey prey physical association physical association false false false 1 A magec1-ctag1b-3 MAGE-C1/CT7 coimmunoprecipitates NY-ESO-1 588007 N/A 588009 unspecified role unspecified role prey prey N/A 588010 unspecified role unspecified role bait bait physical association physical association false false false 1 B ctag1b-magec1-1 MAGE-C1/CT7 coimmunoprecipitates with NY-ESO-1 588007 N/A 588009 unspecified role unspecified role bait bait N/A 588010 unspecified role unspecified role prey prey physical association physical association false false false 1 B magec1-ctag1b-4 MAGE-C1/CT7 colocalizes with NY-ESO-1 588008 N/A 588009 unspecified role unspecified role neutral component neutral component N/A 588010 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2