IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ di_agostino-2006-5 Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Di Agostino S., Strano S., Emiliozzi V., Zerbini V., Mottolese M., Sacchi A., Blandino G., Piaggio G. Apoptosis - Interactions involving proteins with a function related to apoptosis blandino@ifo.it Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Cancer - Interactions investigated in the context of cancer di_agostino-2006-3 Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation. human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Di Agostino S., Strano S., Emiliozzi V., Zerbini V., Mottolese M., Sacchi A., Blandino G., Piaggio G. Apoptosis - Interactions involving proteins with a function related to apoptosis blandino@ifo.it Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Cancer - Interactions investigated in the context of cancer di_agostino-2006-1 Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation. human-colon human colon colon anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Di Agostino S., Strano S., Emiliozzi V., Zerbini V., Mottolese M., Sacchi A., Blandino G., Piaggio G. Apoptosis - Interactions involving proteins with a function related to apoptosis blandino@ifo.it Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Cancer - Interactions investigated in the context of cancer di_agostino-2006-2 Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation. human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Di Agostino S., Strano S., Emiliozzi V., Zerbini V., Mottolese M., Sacchi A., Blandino G., Piaggio G. Apoptosis - Interactions involving proteins with a function related to apoptosis blandino@ifo.it Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Cancer - Interactions investigated in the context of cancer di_agostino-2006-4 Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation. human-mcf10a Human non-transformed breast epithelial cell line mcf10a non-transformed human breast epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Di Agostino S., Strano S., Emiliozzi V., Zerbini V., Mottolese M., Sacchi A., Blandino G., Piaggio G. Apoptosis - Interactions involving proteins with a function related to apoptosis blandino@ifo.it Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Cancer - Interactions investigated in the context of cancer di_agostino-2006-6 Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation. human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion imaging technique imaging technique microscopy immunostaining immunostaining Di Agostino S., Strano S., Emiliozzi V., Zerbini V., Mottolese M., Sacchi A., Blandino G., Piaggio G. Apoptosis - Interactions involving proteins with a function related to apoptosis blandino@ifo.it Cancer Cell (1535-6108) 2006 Only protein-protein interactions 14 imex curation Cancer - Interactions investigated in the context of cancer kat2b_human Histone acetyltransferase KAT2B P300/CBP-associated factor PCAF Lysine acetyltransferase 2B Histone acetyltransferase PCAF KAT2B Spermidine acetyltransferase KAT2B protein protein human Homo sapiens Human MSEAGGAGPGGCGAGAGAGAGPGALPPQPAALPPAPPQGSPCAAAAGGSGACGPATAVAAAGTAEGPGGGGSARIAVKKAQLRSAPRAKKLEKLGVYSACKAEESCKCNGWKNPNPSPTPPRADLQQIIVSLTESCRSCSHALAAHVSHLENVSEEEMNRLLGIVLDVEYLFTCVHKEEDADTKQVYFYLFKLLRKSILQRGKPVVEGSLEKKPPFEKPSIEQGVNNFVQYKFSHLPAKERQTIVELAKMFLNRINYWHLEAPSQRRLRSPNDDISGYKENYTRWLCYCNVPQFCDSLPRYETTQVFGRTLLRSVFTVMRRQLLEQARQEKDKLPLEKRTLILTHFPKFLSMLEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINPPPVAGTISYNSTSSSLEQPNAGSSSPACKASSGLEANPGEKRKMTDSHVLEEAKKPRVMGDIPMELINEVMSTITDPAAMLGPETNFLSAHSARDEAARLEERRGVIEFHVVGNSLNQKPNKKILMWLVGLQNVFSHQLPRMPKEYITRLVFDPKHKTLALIKDGRVIGGICFRMFPSQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHDILNFLTYADEYAIGYFKKQGFSKEIKIPKTKYVGYIKDYEGATLMGCELNPRIPYTEFSVIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKDGVRQIPIESIPGIRETGWKPSGKEKSKEPRDPDQLYSTLKSILQQVKSHQSAWPFMEPVKRTEAPGYYEVIRFPMDLKTMSERLKNRYYVSKKLFMADLQRVFTNCKEYNPPESEYYKCANILEKFFFSKIKEAGLIDK p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD nfya_human Nuclear transcription factor Y subunit alpha NFYA Nuclear transcription factor Y subunit A CAAT box DNA-binding protein subunit A protein protein human Homo sapiens Human MEQYTANSNSSTEQIVVQAGQIQQQQQGGVTAVQLQTEAQVASASGQQVQTLQVVQGQPLMVQVSGGQLITSTGQPIMVQAVPGGQGQTIMQVPVSGTQGLQQIQLVPPGQIQIQGGQAVQVQGQQGQTQQIIIQQPQTAVTAGQTQTQQQIAVQGQQVAQTAEGQTIVYQPVNADGTILQQVTVPVSGMITIPAASLAGAQIVQTGANTNTTSSGQGTVTVTLPVAGNVVNSGGMVMMVPGAGSVPAIQRIPLPGAEMLEEEPLYVNAKQYHRILKRRQARAKLEAEGKIPKERRKYLHESRHRHAMARKRGEGGRFFSPKEKDSPHMQDPNQADEEAMTQIIRVS nfyb_human Nuclear transcription factor Y subunit beta NFYB HAP3 Nuclear transcription factor Y subunit B CAAT box DNA-binding protein subunit B protein protein human Homo sapiens Human MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS hdac1_human Histone deacetylase 1 HDAC1 RPD3L1 protein protein human Homo sapiens Human MAQTQGTRRKVCYYYDGDVGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKANAEEMTKYHSDDYIKFLRSIRPDNMSEYSKQMQRFNVGEDCPVFDGLFEFCQLSTGGSVASAVKLNKQQTDIAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNYPLRDGIDDESYEAIFKPVMSKVMEMFQPSAVVLQCGSDSLSGDRLGCFNLTIKGHAKCVEFVKSFNLPMLMLGGGGYTIRNVARCWTYETAVALDTEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTNEYLEKIKQRLFENLRMLPHAPGVQMQAIPEDAIPEESGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA nfya-tp53 511049 N/A 511055 unspecified role unspecified role prey prey N/A 511056 unspecified role unspecified role prey prey P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 511057 unspecified role unspecified role bait bait association association false false false 5 A, B Mutant p53 and NF-YA are needed for binding of p300. The H-175#41 cells are H1299 containing ponasterone-inducible mutp53 (arg175his). This complex was formed when the cells were induced with ponasterone and transfected with vectors for NF-YA and p300. Transfection p53, NF-YA and p300 were overexpressed. In the presence of p300 both NF-YA and mutant p53 (arg175his) were acetylated. nfya-tp53-1 511049 N/A 511057 unspecified role unspecified role bait bait N/A 511056 unspecified role unspecified role prey prey physical association physical association false false false 5 A Mutant p53 and NF-YA are needed for binding of p300. H-175#41 cells are H1299 cells containing ponasterone-inducible mutant p53 (arg173his). This complex was formed when cells were not induced with ponasterone. NF-YA and p300 were overexpressed. Transfection tp53-nfya-5 511049 N/A 511057 unspecified role unspecified role prey prey N/A 511055 unspecified role unspecified role prey prey N/A 511056 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false association association false false false 5 A, B Mutant p53 and NF-YA are needed for binding of p300. The H-175#41 cells are H1299 cells which contain ponasterone-inducible mutant p53His175. P53, NF-YA and p300 were overexpressed. Transfection. This complex was formed when the cells were induced with ponasterone and transfected with vectors for NF-YA and p300. In the presence of p300 both NF-YA and mutant p53 (arg175his) were acetylated. tp53-nfya-6 511049 N/A 511056 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 511058 unspecified role unspecified role prey prey N/A 511057 unspecified role unspecified role prey prey association association false false false S1 B Thic complex was formed in H1299 cells with ponasterone induceable wild-type p53 or mutant p53 (arg175his). Transfection The cells were tranfected with NF-YA vector, which was overexpressed. P53 was also overepxressed. nfya-tp53-2 511049 N/A 511057 unspecified role unspecified role prey prey N/A 511056 unspecified role unspecified role prey prey P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 511058 unspecified role unspecified role bait bait association association false false false S1 B Thic complex was formed in H1299 cells with ponasterone induceable wild-type p53 or mutant p53 (arg175his). Transfection The cells were tranfected with NF-YA vector, which was overexpressed. P53 was also overexpressed. nfya-tp53-3 511049 N/A 511058 unspecified role unspecified role bait bait N/A 511057 unspecified role unspecified role prey prey physical association physical association false false false S1 B Thic complex was formed in H1299 cells which are p53-null. Transfection The cells were tranfected with NF-YA vector, which was overexpressed. tp53-nfya-2 511050 N/A 511056 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 511057 unspecified role unspecified role prey prey N/A 511058 unspecified role unspecified role prey prey association association false false false 1 A SKBR3 cells harbour endogenous mutant p53 (arg175his). Adriamycin treatment does not affect formation of the complex. tp53-nfya-3 511050 N/A 511057 unspecified role unspecified role prey prey N/A 511055 unspecified role unspecified role prey prey N/A 511059 unspecified role unspecified role prey prey N/A 511056 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false association association false false false 5 C This complex was found predominantly in cells treated with adriamycin. SKBR3 cells harbour endogenous mutant p53 (arg175his). tp53-nfya-4 511050 N/A 511057 unspecified role unspecified role prey prey N/A 511059 unspecified role unspecified role prey prey N/A 511056 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false association association false false false 5 C This complex was found in the absence of adriamycin. SKBR3 cells harbour endogenous mutant p53 (arg175his). tp53-nfya-7 511050 N/A 511056 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 511057 unspecified role unspecified role prey prey physical association physical association false false false S1 C SKBR3 cells harbour mutant p53 (arg175his). tp53-nfya 511051 N/A 511056 unspecified role unspecified role bait bait P04637:p.[Arg273His;Pro309Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 511057 unspecified role unspecified role prey prey N/A 511058 unspecified role unspecified role prey prey association association false false false 1 A SW480 cells harbour mutant p53 (arg273his-leu309ser). Adriamycin treatment does not affect formation of the complex. tp53-nfya-1 511052 N/A 511056 unspecified role unspecified role bait bait P04637:p.Arg273His mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 511057 unspecified role unspecified role prey prey N/A 511058 unspecified role unspecified role prey prey association association false false false 1 A HT-29 cells harbour mutant p53 (arg273his). Adriamycin treatment does not affect formation of the complex. tp53-hdac1 511053 N/A 511059 unspecified role unspecified role prey prey N/A 511056 unspecified role unspecified role bait bait physical association physical association false false false 5 D wild-type p53 in MCF10 cells treated with adriamycin interacted preferentially with HDAC1 and not p300. tp53-nfya-8 511054 N/A 511056 unspecified role unspecified role neutral component neutral component P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 511057 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S1 B SKBR3 cells harbour mutant p53 (arg175his). RNAi knockdown of mutant p53 (arg175his) did not affect the localization of NF-YA