IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mann-2006-1 Overexpression of Tissue Transglutaminase Leads to Constitutive Activation of Nuclear Factor-{kappa}B in Cancer Cells: Delineation of a Novel Pathway. human-mdapanc28 Homo sapiens pancreatic adenocarcinoma cell line mdapanc28 Human pancreatic adenocarcinoma cell line The cell line expresses trypsin and ribonuclease RNA, which are considered to be acinar cell markers, and carbonic anhydrase II (CAII), which is considered to be a duct-cell markers. The histological appearance of xenografts in nude mice was similar to that of the tumor from which the cell line was established. The chromosome number varied between 46 and 60. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mann AP., Verma A., Sethi G., Manavathi B., Wang H., Fok JY., Kunnumakkara AB., Kumar R., Aggarwal BB., Mehta K. Cancer - Interactions investigated in the context of cancer kmehta@mdanderson.org Cancer Res. (0008-5472) 2006 Only protein-protein interactions imex curation mann-2006-2 Overexpression of Tissue Transglutaminase Leads to Constitutive Activation of Nuclear Factor-{kappa}B in Cancer Cells: Delineation of a Novel Pathway. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mann AP., Verma A., Sethi G., Manavathi B., Wang H., Fok JY., Kunnumakkara AB., Kumar R., Aggarwal BB., Mehta K. Cancer - Interactions investigated in the context of cancer kmehta@mdanderson.org Cancer Res. (0008-5472) 2006 Only protein-protein interactions imex curation mann-2006-3 Overexpression of Tissue Transglutaminase Leads to Constitutive Activation of Nuclear Factor-{kappa}B in Cancer Cells: Delineation of a Novel Pathway. human-bxpc3 Homo sapiens primary pancreatic adenocarcinoma cell line bxpc3 BxPC-3 Human primary pancreatic adenocarcinoma cell line Derived from a 61 year old female with a primary adenocarcinoma of the pancreas. BxPC-3 cells produce mucin and the tumour in a nude mouse is moderately well to poorly differentiated like the primary adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mann AP., Verma A., Sethi G., Manavathi B., Wang H., Fok JY., Kunnumakkara AB., Kumar R., Aggarwal BB., Mehta K. Cancer - Interactions investigated in the context of cancer kmehta@mdanderson.org Cancer Res. (0008-5472) 2006 Only protein-protein interactions imex curation mann-2006-4 Overexpression of Tissue Transglutaminase Leads to Constitutive Activation of Nuclear Factor-{kappa}B in Cancer Cells: Delineation of a Novel Pathway. human-mdapanc28 Homo sapiens pancreatic adenocarcinoma cell line mdapanc28 Human pancreatic adenocarcinoma cell line The cell line expresses trypsin and ribonuclease RNA, which are considered to be acinar cell markers, and carbonic anhydrase II (CAII), which is considered to be a duct-cell markers. The histological appearance of xenografts in nude mice was similar to that of the tumor from which the cell line was established. The chromosome number varied between 46 and 60. confocal microscopy confocal microscopy predetermined predetermined participant predetermined Mann AP., Verma A., Sethi G., Manavathi B., Wang H., Fok JY., Kunnumakkara AB., Kumar R., Aggarwal BB., Mehta K. Cancer - Interactions investigated in the context of cancer kmehta@mdanderson.org Cancer Res. (0008-5472) 2006 Only protein-protein interactions imex curation tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS tgm2_human Protein-glutamine gamma-glutamyltransferase 2 TGM2 Tissue transglutaminase Transglutaminase C Transglutaminase-2 Transglutaminase H Erythrocyte transglutaminase Heart G alpha(h) Isopeptidase TGM2 Protein G alpha(h) Protein-glutamine deamidase TGM2 Protein-glutamine dopaminyltransferase TGM2 Protein-glutamine histaminyltransferase TGM2 Protein-glutamine noradrenalinyltransferase TGM2 Protein-glutamine serotonyltransferase TGM2 Transglutaminase II protein protein human Homo sapiens Human MAEELVLERCDLELETNGRDHHTADLCREKLVVRRGQPFWLTLHFEGRNYEASVDSLTFSVVTGPAPSQEAGTKARFPLRDAVEEGDWTATVVDQQDCTLSLQLTTPANAPIGLYRLSLEASTGYQGSSFVLGHFILLFNAWCPADAVYLDSEEERQEYVLTQQGFIYQGSAKFIKNIPWNFGQFEDGILDICLILLDVNPKFLKNAGRDCSRRSSPVYVGRVVSGMVNCNDDQGVLLGRWDNNYGDGVSPMSWIGSVDILRRWKNHGCQRVKYGQCWVFAAVACTVLRCLGIPTRVVTNYNSAHDQNSNLLIEYFRNEFGEIQGDKSEMIWNFHCWVESWMTRPDLQPGYEGWQALDPTPQEKSEGTYCCGPVPVRAIKEGDLSTKYDAPFVFAEVNADVVDWIQQDDGSVHKSINRSLIVGLKISTKSVGRDEREDITHTYKYPEGSSEEREAFTRANHLNKLAEKEETGMAMRIRVGQSMNMGSDFDVFAHITNNTAEEYVCRLLLCARTVSYNGILGPECGTKYLLNLNLEPFSEKSVPLCILYEKYRDCLTESNLIKVRALLVEPVINSYLLAERDLYLENPEIKIRILGEPKQKRKLVAEVSLQNPLPVALEGCTFTVEGAGLTEEQKTVEIPDPVEAGEEVKVRMDLLPLHMGLHKLVVNFESDKLKAVKGFRNVIIGPA ikba_human NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 protein protein human Homo sapiens Human MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL nfkbia-rela-1 IkappaBalpha coimmunoprecipitates TG2 and p65 in MDAPanc28 lysates 669106 N/A 669110 unspecified role unspecified role prey prey N/A 669111 unspecified role unspecified role prey prey N/A 669112 unspecified role unspecified role bait bait association association false false false 4 A A23187 - Treatment with the calcium ionophore, A23187, (which causes activation of TG2), enhanced this interaction. nfkbia-rela-2 p65 coimmunoprecipitates with TG2 in MDAPanc28 lysates 669106 N/A 669110 unspecified role unspecified role prey prey N/A 669111 unspecified role unspecified role bait bait physical association physical association false false false 4 C Note that the blot in Fig. 4 C only shows that p65 is coimmunoprecipitated with TG2 and IkappaBalpha is not shown to coimmunoprecipitate. nfkb1-nfkb1-2 IkappaBalpha dimers form in cytoplasmic extract from MDAPanc28 669106 N/A 669112 unspecified role unspecified role prey prey Stoichiometry: 1.0 N/A 669112 unspecified role unspecified role bait bait Stoichiometry: 1.0 physical association physical association false false false 4 D A23187 - Treatment with the calcium ionophore (A23187), which activates TG2, augmented dimer formation and results in the apppearance of another polymeric IKappaBalpha band. The dimeric form of IkappaBalpha was also seen when IKappaBalpha immunoprecipitates were labeled with BPA and probed with HRP-conjugated streptavidin. nfkb1-nfkb1-3 IkappaBalpha polymeric form appears in cytoplasmic extract from MDAPanc28 cells treated with A23187 669106 N/A 669112 unspecified role unspecified role bait bait N/A 669112 unspecified role unspecified role prey prey physical association physical association false false false 4 D A23187 - Treatment with the calcium ionophore (A23187), which activates TG2, augmented dimer formation and results in the apppearance of another polymeric IKappaBalpha band. This polymeric form of IkappaBalpha is of higher order than the dimeric form also seen, as A23187 treatment resulted in the appearance of another polymeric band. The polymeric form of IkappaBalpha was also seen when IKappaBalpha immunoprecipitates were labeled with BPA and probed with HRP-conjugated streptavidin. nfkbia-rela-3 IKappaBalpha coimmunoprecipitates p65 in MCF7 lysates 669107 N/A 669112 unspecified role unspecified role bait bait N/A 669110 unspecified role unspecified role prey prey physical association physical association false false false 4 A Unlike in the MDAPanc28 lysates, IkappaBalpha did not also coimmunoprecipitate TG2. tgm2-rela TG2 coimmunoprecipitates P65 in BXPC-3 lysates 669108 N/A 669111 unspecified role unspecified role bait bait N/A 669110 unspecified role unspecified role prey prey physical association physical association false false false 4 C rela-tgm2 P65 and TG2 colocalize in MDAPanc28 cells 669109 N/A 669110 unspecified role unspecified role neutral component neutral component N/A 669111 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4 B A23187 - Treatment with the calcium ionophore, A23187, which activates TG2, enahnced complex formation and increased translocation of the complex to the nucleus.