IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ amir-2006-1 MSF-A interacts with hypoxia-inducible factor-1alpha and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Amir S., Wang R., Matzkin H., Simons JW., Mabjeesh NJ. Cancer - Interactions investigated in the context of cancer The cell line used was cultured under normoxic or hypoxic conditions. Figure 4 (D). nicolam@tasmc.health.gov.il Cancer Res. (0008-5472) 2006 Only protein-protein interactions imex curation amir-2006-2 MSF-A interacts with hypoxia-inducible factor-1alpha and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Amir S., Wang R., Matzkin H., Simons JW., Mabjeesh NJ. Cancer - Interactions investigated in the context of cancer The cell line used was cultured under normoxic or hypoxic conditions. Figure 4 (D). nicolam@tasmc.health.gov.il Cancer Res. (0008-5472) 2006 Only protein-protein interactions imex curation amir-2006-3 MSF-A interacts with hypoxia-inducible factor-1alpha and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis. human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Amir S., Wang R., Matzkin H., Simons JW., Mabjeesh NJ. Cancer - Interactions investigated in the context of cancer The cell line used was cultured under normoxic or hypoxic conditions. Figure 4 (D). nicolam@tasmc.health.gov.il Cancer Res. (0008-5472) 2006 Only protein-protein interactions imex curation amir-2006-4 MSF-A interacts with hypoxia-inducible factor-1alpha and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis. human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Amir S., Wang R., Matzkin H., Simons JW., Mabjeesh NJ. Cancer - Interactions investigated in the context of cancer The cell line used was cultured under normoxic or hypoxic conditions. Figure 4 (D). nicolam@tasmc.health.gov.il Cancer Res. (0008-5472) 2006 Only protein-protein interactions imex curation hif1a_human Hypoxia-inducible factor 1-alpha ARNT-interacting protein Member of PAS protein 1 Basic-helix-loop-helix-PAS protein MOP1 HIF1A MOP1 BHLHE78 PASD8 PAS domain-containing protein 8 Class E basic helix-loop-helix protein 78 protein protein human Homo sapiens Human MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN q9uhd8-1 Septin-9 SEPTIN9 KIAA0991 MLL septin-like fusion protein MSF-A MSF Ovarian/Breast septin SEPT9 Septin D1 Epsilon MSF-A protein protein human Homo sapiens Human MKKSYSGGTRTSSGRLRRLGDSSGPALKRSFEVEEVETPNSTPPRRVQTPLLRATVASSTQKFQDLGVKNSEPSARHVDSLSQRSPKASLRRVELSGPKAAEPVSRRTELSIDISSKQVENAGAIGPSRFGLKRAEVLGHKTPEPAPRRTEITIVKPQESAHRRMEPPASKVPEVPTAPATDAAPKRVEIQMPKPAEAPTAPSPAQTLENSEPAPVSQLQSRLEPKPQPPVAEATPRSQEATEAAPSCVGDMADTPRDAGLKQAPASRNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGMEEKEPEAPEM sept9-q53xp6 Immunoprecipitation analysis reveals interaction between the MSF-A and HIF-1 alpha proteins. 329015 N/A 329019 unspecified role unspecified role prey prey N/A 329020 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false The authors note that this interaction was observed under normoxic conditions. q53xp6-sept9 Immunoprecipitation analysis reveals interaction between the HIF-1 alpha and MSF-A proteins. 329016 N/A 329019 unspecified role unspecified role bait bait N/A 329020 unspecified role unspecified role prey prey n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The authors note that this interaction was observed under normoxic conditions. sept9-q53xp6-1 Co-localisation analysis reveals interaction between the MSF-A and HIF-1 alpha proteins. 329017 N/A 329019 unspecified role unspecified role neutral component neutral component N/A 329020 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false The proteins colocalised to the nucleus. Under normoxic conditions Q53XP6 was barely detectable but accumulated under hypoxic conditions. q53xp6-sept9-1 Immunoprecipitation analysis reveals interaction between the MSF-A and HIF-1 alpha proteins. 329018 N/A 329019 unspecified role unspecified role bait bait N/A 329020 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The authors suggest that endogenous HIF-1 alpha associates with the endogenous MSF-A protein with higher affinity under normoxic conditions than under hypoxic conditions, in spite of higher content of HIF-1 alpha during hypoxy.