IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yu-2006-1 Requirement for CDK4 kinase function in breast cancer. mouse-mammary gland Mus musculus mammary gland mammary_gland mammary gland anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yu Q., Sicinska E., Geng Y., Ahnstrom M., Zagozdzon A., Kong Y., Gardner H., Kiyokawa H., Harris LN., Stal O., Sicinski P. Cancer - Interactions investigated in the context of cancer peter_sicinski@dfci.harvard.edu Cancer Cell (1535-6108) 2006 Only protein-protein interactions 6 imex curation ccnd1_mouse G1/S-specific cyclin-D1 Ccnd1 Cyl-1 protein protein mouse Mus musculus Mouse MEHQLLCCEVETIRRAYPDTNLLNDRVLRAMLKTEETCAPSVSYFKCVQKEIVPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPLKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEADENKQTIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAMQGLNLGSPNNFLSCYRTTHFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNVDPKATEEEGEVEEEAGLACTPTDVRDVDI cdk6_mouse Cyclin-dependent kinase 6 Serine/threonine-protein kinase PLSTIRE Cdkn6 Cdk6 Crk2 Cell division protein kinase 6 CR2 protein kinase protein protein mouse Mus musculus Mouse MEKDSLSRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTSEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDIIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYGALNHPYFQDLERYKDNLNSHLPSNQSTSELNTA cdk4_mouse Cyclin-dependent kinase 4 Cell division protein kinase 4 PSK-J3 CRK3 Cdk4 Crk3 protein protein mouse Mus musculus Mouse MAATRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGAAGGGLPVSTVREVALLRRLEAFEHPNVVRLMDVCATSRTDRDIKVTLVFEHIDQDLRTYLDKAPPPGLPVETIKDLMRQFLSGLDFLHANCIVHRDLKPENILVTSNGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPREVSLPRGAFAPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKEESDAE cdn1b_mouse Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 Cdkn1b protein protein mouse Mus musculus Mouse MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVNHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGRYEWQEVERGSLPEFYYRPPRPPKSACKVLAQESQDVSGSRQAVPLIGSQANSEDRHLVDQMPDSSDNPAGLAEQCPGMRKRPAAEDSSSQNKRANRTEENVSDGSPNAGTVEQTPKKPGLRRQT cdk2_mouse Cyclin-dependent kinase 2 Cdk2 Cell division protein kinase 2 Cdkn2 protein protein mouse Mus musculus Mouse MENFQKVEKIGEGTYGVVYKAKNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINAEGSIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMHLVCTQHHAKCCGEHRRNGRHSLCPLCSYLEVAASQGGGMTAVSAPHPVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL ccnd3_mouse G1/S-specific cyclin-D3 Ccnd3 Cyl-3 protein protein mouse Mus musculus Mouse MELLCCEGTRHAPRAGPDPRLLGDQRVLQSLLRLEERYVPRASYFQCVQKEIKPHMRKMLAYWMLEVCEEQRCEEDVFPLAMNYLDRYLSCVPTRKAQLQLLGTVCLLLASKLRETTPLTIEKLCIYTDQAVAPWQLREWEVLVLGKLKWDLAAVIAHDFLALILHRLSLPSDRQALVKKHAQTFLALCATDYTFAMYPPSMIATGSIGAAVLGLGACSMSADELTELLAGITGTEVDCLRACQEQIEAALRESLREAAQTAPSPVPKAPRGSSSQGPSQTSTPTDVTAIHL ccnd2_mouse G1/S-specific cyclin-D2 Ccnd2 Cyl-2 protein protein mouse Mus musculus Mouse MELLCCEVDPVRRAVPDRNLLEDRVLQNLLTIEERYLPQCSYFKCVQKDIQPYMRRMVATWMLEVCEEQKCEEEVFPLAMNYLDRFLAGVPTPKTHLQLLGAVCMFLASKLKETIPLTAEKLCIYTDNSVKPQELLEWELVVLGKLKWNLAAVTPHDFIEHILRKLPQQKEKLSLIRKHAQTFIALCATDFKFAMYPPSMIATGSVGAAICGLQQDDEVNTLTCDALTELLAKITHTDVDCLKACQEQIEALLLNSLQQFRQEQHNAGSKSVEDPDQATTPTDVRDVDL ccnd1-cdk2 509336 N/A 509337 unspecified role unspecified role bait bait N/A 509338 unspecified role unspecified role prey prey N/A 509339 unspecified role unspecified role prey prey N/A 509340 unspecified role unspecified role prey prey N/A 509341 unspecified role unspecified role prey prey association association false false false 1 B This interaction was found in mammary glands of pregnant wild-type mice and in mammary glands of wild-type female mice that were hormonally stimulated to undergo lobuloalveolar development. In Cdk4 null mammary glands that were stimulated by hormones to undergo lobuloalveolar development, cyclin D1 associated with all of these proteins except for Cdk4. ccnd1-cdk4-1 509336 N/A 509337 unspecified role unspecified role bait bait N/A 509338 unspecified role unspecified role prey prey N/A 509339 unspecified role unspecified role prey prey association association false false false 3 B These complexes form in mammary glands from day 13.5 mouse embryos. cdk4-ccnd1-8 509336 N/A 509339 unspecified role unspecified role bait bait N/A 509337 unspecified role unspecified role prey prey N/A 509342 unspecified role unspecified role prey prey N/A 509343 unspecified role unspecified role prey prey association association false false false 3 C These complexes form in mammary glands from day 13.5 mouse embryos. The band for cyclin D3 is lighter than for the other forms of cyclin D. There is a double band for cyclin D1. ccnd1-cdk4-3 509336 N/A 509337 unspecified role unspecified role bait bait N/A 509339 unspecified role unspecified role prey prey physical association physical association false false false 3 B These complexes form in mammary tumours arising in MMTV-ErbB-2 mice. cdk4-ccnd1-9 509336 N/A 509339 unspecified role unspecified role bait bait N/A 509337 unspecified role unspecified role prey prey physical association physical association false false false 3 C These complexes form in mammary tumours arising in MMTV-ErbB-2 mice. There is a double band for cyclin D1. ccnd1-cdk2-1 509336 N/A 509340 unspecified role unspecified role prey prey N/A 509337 unspecified role unspecified role bait bait N/A 509338 unspecified role unspecified role prey prey N/A 509341 unspecified role unspecified role prey prey association association false false false 1 B right panel This interaction was in mammary glands of CDK4 null female mice that were hormonally stimulated to undergo lobuloalveolar development.