MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint barral-2005-1 The interaction of the hnRNP family member E1B-AP5 with p53. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Barral PM., Rusch A., Turnell AS., Gallimore PH., Byrd PJ., Dobner T., Grand RJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Pull down experiments were performed using lysates from A549 cells, HCT116 cells, C33A cells and AdE1HEK293 cells (HEK293 cells transformed with Ad5 early region 1 DNA), and Ad12E1HER2 cells (human embryonic retinoblasts (HER2 transformed with the Ad12 E1 region). r.j.a.grand@bham.ac.uk FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer barral-2005-6 The interaction of the hnRNP family member E1B-AP5 with p53. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Barral PM., Rusch A., Turnell AS., Gallimore PH., Byrd PJ., Dobner T., Grand RJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Pull down experiments were performed using lysates from A549 cells, HCT116 cells, C33A cells and AdE1HEK293 cells (HEK293 cells transformed with Ad5 early region 1 DNA), and Ad12E1HER2 cells (human embryonic retinoblasts (HER2 transformed with the Ad12 E1 region). r.j.a.grand@bham.ac.uk FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer barral-2005-2 The interaction of the hnRNP family member E1B-AP5 with p53. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barral PM., Rusch A., Turnell AS., Gallimore PH., Byrd PJ., Dobner T., Grand RJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Pull down experiments were performed using lysates from A549 cells, HCT116 cells, C33A cells and AdE1HEK293 cells (HEK293 cells transformed with Ad5 early region 1 DNA), and Ad12E1HER2 cells (human embryonic retinoblasts (HER2 transformed with the Ad12 E1 region). r.j.a.grand@bham.ac.uk FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer barral-2005-3 The interaction of the hnRNP family member E1B-AP5 with p53. human-er2 Homo sapiens embryonic retinoblasts cells line HER2 er2 Human embryonic retinoblast cell line HER2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barral PM., Rusch A., Turnell AS., Gallimore PH., Byrd PJ., Dobner T., Grand RJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Pull down experiments were performed using lysates from A549 cells, HCT116 cells, C33A cells and AdE1HEK293 cells (HEK293 cells transformed with Ad5 early region 1 DNA), and Ad12E1HER2 cells (human embryonic retinoblasts (HER2 transformed with the Ad12 E1 region). r.j.a.grand@bham.ac.uk FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer barral-2005-4 The interaction of the hnRNP family member E1B-AP5 with p53. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barral PM., Rusch A., Turnell AS., Gallimore PH., Byrd PJ., Dobner T., Grand RJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Pull down experiments were performed using lysates from A549 cells, HCT116 cells, C33A cells and AdE1HEK293 cells (HEK293 cells transformed with Ad5 early region 1 DNA), and Ad12E1HER2 cells (human embryonic retinoblasts (HER2 transformed with the Ad12 E1 region). r.j.a.grand@bham.ac.uk FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer barral-2005-5 The interaction of the hnRNP family member E1B-AP5 with p53. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barral PM., Rusch A., Turnell AS., Gallimore PH., Byrd PJ., Dobner T., Grand RJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Pull down experiments were performed using lysates from A549 cells, HCT116 cells, C33A cells and AdE1HEK293 cells (HEK293 cells transformed with Ad5 early region 1 DNA), and Ad12E1HER2 cells (human embryonic retinoblasts (HER2 transformed with the Ad12 E1 region). r.j.a.grand@bham.ac.uk FEBS Lett. (0014-5793) 2005 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD hnrl1_human Heterogeneous nuclear ribonucleoprotein U-like protein 1 E1BAP5 HNRNPUL1 HNRPUL1 Adenovirus early region 1B-associated protein 5 E1B-55 kDa-associated protein 5 protein protein human Homo sapiens Human MDVRRLKVNELREELQRRGLDTRGLKAELAERLQAALEAEEPDDERELDADDEPGRPGHINEEVETEGGSELEGTAQPPPPGLQPHAEPGGYSGPDGHYAMDNITRQNQFYDTQVIKQENESGYERRPLEMEQQQAYRPEMKTEMKQGAPTSFLPPEASQLKPDRQQFQSRKRPYEENRGRGYFEHREDRRGRSPQPPAEEDEDDFDDTLVAIDTYNCDLHFKVARDRSSGYPLTIEGFAYLWSGARASYGVRRGRVCFEMKINEEISVKHLPSTEPDPHVVRIGWSLDSCSTQLGEEPFSYGYGGTGKKSTNSRFENYGDKFAENDVIGCFADFECGNDVELSFTKNGKWMGIAFRIQKEALGGQALYPHVLVKNCAVEFNFGQRAEPYCSVLPGFTFIQHLPLSERIRGTVGPKSKAECEILMMVGLPAAGKTTWAIKHAASNPSKKYNILGTNAIMDKMRVMGLRRQRNYAGRWDVLIQQATQCLNRLIQIAARKKRNYILDQTNVYGSAQRRKMRPFEGFQRKAIVICPTDEDLKDRTIKRTDEEGKDVPDHAVLEMKANFTLPDVGDFLDEVLFIELQREEADKLVRQYNEEGRKAGPPPEKRFDNRGGGGFRGRGGGGGFQRYENRGPPGGNRGGFQNRGGGSGGGGNYRGGFNRSGGGGYSQNRWGNNNRDNNNSNNRGSYNRAPQQQPPPQQPPPPQPPPQQPPPPPSYSPARNPPGASTYNKNSNIPGSSANTSTPTVSSYSPPQPSYSQPPYNQGGYSQGYTAPPPPPPPPPAYNYGSYGGYNPAPYTPPPPPTAQTYPQPSYNQYQQYAQQWNQYYQNQGQWPPYYGNYDYGSYSGNTQGGTSTQ hnrpul1-tp53 GST-E1B-AP5 pulls down p53 from A549 lysates 189068 N/A 189074 unspecified role unspecified role prey prey human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 189075 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false 2 A hnrpul1-tp53-1 GST-E1B-AP5 pulls down p53 from HCT116 lysates 189068 N/A 189074 unspecified role unspecified role prey prey human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 N/A 189075 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false 2 A hnrpul1-tp53-2 GST-E1B-AP5 pulls down mutant p53 from C33A lysates 189068 N/A 189075 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 189074 unspecified role unspecified role prey prey human-c33a Homo sapiens epihelial cervix carcinoma cells c33a C33A cells epithelial-like cells, grown as monolayer; derived from cervical carcinoma physical association physical association false false false 2 A C33A cells express mutant p53. hnrpul1-tp53-3 GST-E1B-AP5 pulls down mutant p53 from Ad5E1HEK293 lysates 189068 N/A 189074 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 189075 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false 2 A The HEK293 cells (Ad5293) from which lysates were prepared, were transformed with the Ad5 early region 1 DNA. hnrpul1-tp53-4 GST-E1B-AP5 pulls down mutant p53 from Ad12E1HER2 lysates 189068 N/A 189074 unspecified role unspecified role prey prey human-er2 Homo sapiens embryonic retinoblasts cells line HER2 er2 Human embryonic retinoblast cell line HER2 N/A 189075 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false 2 A The human embryonic retinoblast cell line HER2 (Ad5293) from which lysates were prepared, was transformed with the Ad12 E1 region. tp53-hrnpul1 GST-p53 pulls down E1B-AP5 in vitro 189069 N/A 189074 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 189075 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false 3 Interaction can be seen with the C-terminal and central regions and to a somewhat lesser extent with the N-terminal region. (Densitometric scanning of the autoradiograph indicates that 8%, 2%, 9% and 5% of the input of full length, N-terminal fragment, central fragment and C-terminal fragment, respectively, bound to the GST-p53.) The C-terminal and central region polypeptides of E1B-AP5 overlap from amino acids 395-732 and it is possible that these residues encompass a major binding site for p53. tp53-hrnpul1-1 GST-p53 (aa318-393) pulls down E1B-AP5 in vitro 189069 N/A 189074 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) N/A 189075 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false 4 The results indicate that the major site of interaction p53 lies C-terminal to aa 318. tp53-hnrpul1 p53 coimmunoprecipitates E1B-AP5 in MCF7 lysates 189070 N/A 189075 unspecified role unspecified role prey prey N/A 189074 unspecified role unspecified role bait bait physical association physical association false false false 2 B tp53-hnrpul1-1 p53 coimmunoprecipitates E1B-AP5 in Ad12E1HER2 lysates 189071 N/A 189074 unspecified role unspecified role bait bait N/A 189075 unspecified role unspecified role prey prey physical association physical association false false false 2 B hrnpul1-tp53 p53 coimmunoprecipitates with E1B-AP5 in 293 lysates 189072 N/A 189075 unspecified role unspecified role bait bait N/A 189074 unspecified role unspecified role prey prey physical association physical association false false false 2 C hrnpul1-tp53-1 p53 coimmunoprecipitates E1B-AP5 in HCT116 lysates 189073 N/A 189075 unspecified role unspecified role bait bait N/A 189074 unspecified role unspecified role prey prey physical association physical association false false false 2 C