IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lin-2004-4 chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Cancer - Interactions investigated in the context of cancer Apoptosis - Interactions involving proteins with a function related to apoptosis shihh@nhri.org.tw Mol. Cell. Biol. (0270-7306) 2004 IMEx Origin of Daxx and AR inferred from sequence length. Only protein-protein interactions imex curation imex curation lin-2004-5 human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Cancer - Interactions investigated in the context of cancer Apoptosis - Interactions involving proteins with a function related to apoptosis shihh@nhri.org.tw Mol. Cell. Biol. (0270-7306) 2004 IMEx Origin of Daxx and AR inferred from sequence length. Only protein-protein interactions imex curation imex curation lin-2004-1 Negative modulation of androgen receptor transcriptional activity by Daxx. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer shihh@nhri.org.tw Mol. Cell. Biol. (0270-7306) 2004 IMEx Origin of Daxx and AR inferred from sequence length. Only protein-protein interactions imex curation imex curation lin-2004-2 Negative modulation of androgen receptor transcriptional activity by Daxx. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer shihh@nhri.org.tw Mol. Cell. Biol. (0270-7306) 2004 IMEx Origin of Daxx and AR inferred from sequence length. Only protein-protein interactions imex curation imex curation lin-2004-3 Negative modulation of androgen receptor transcriptional activity by Daxx. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer shihh@nhri.org.tw Mol. Cell. Biol. (0270-7306) 2004 IMEx Origin of Daxx and AR inferred from sequence length. Only protein-protein interactions imex curation imex curation andr_human Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 protein protein human Homo sapiens Human MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ daxx_human Death domain-associated protein 6 DAXX BING2 DAP6 Daxx Fas death domain-associated protein ETS1-associated protein 1 protein protein human Homo sapiens Human MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPSGMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCKKSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCIPSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD daxx-ar-5 Interaction of DAXX and AR (sumoylated) detected by CoIP. 194921 N/A 194926 unspecified role unspecified role bait bait N/A 194927 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false prostate cancer FIG. 1A. ar-daxx-1 194922 N/A 194926 unspecified role unspecified role prey prey N/A 194927 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1B daxx-ar-1 Interaction of DAXX and AR detected by Y2H. 194923 N/A 194926 unspecified role unspecified role prey prey n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false dna binding domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P10275:p.[Lys388Arg;Lys521Arg] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 194927 unspecified role unspecified role bait bait physical association physical association false false false prostate cancer FIG. 5., Fig 7 daxx-ar-3 Interaction of DAXX and AR detected by GST pull down. 194924 N/A 194927 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 194926 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false prostate cancer FIG. 1D daxx-ar-4 Interaction of DAXX and AR detected by CoIP. 194925 N/A 194927 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 194926 unspecified role unspecified role prey prey n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false dna binding domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false prostate cancer Fig. 1A, 5B