IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-2004f-1 A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wang L., Bhattacharyya N., Chelsea DM., Escobar PF., Banerjee S. Cancer - Interactions investigated in the context of cancer PMID 11467963 indicates that the recombinant DNA polBeta used is of human origin and gives the range for DNA polBeta Delta. A search for MGC5306 in NCBI Entrez found CAG33650 containing a reference to the TrEMBL entry Q6I9Y6, which corresponds to primary UniProtKB accession number Q9H5J8. banerjs@ccf.org HeLa cell cDNA library Cancer Res. (0008-5472) 2004 wang-2004f-2 A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang L., Bhattacharyya N., Chelsea DM., Escobar PF., Banerjee S. Cancer - Interactions investigated in the context of cancer PMID 11467963 indicates that the recombinant DNA polBeta used is of human origin and gives the range for DNA polBeta Delta. A search for MGC5306 in NCBI Entrez found CAG33650 containing a reference to the TrEMBL entry Q6I9Y6, which corresponds to primary UniProtKB accession number Q9H5J8. banerjs@ccf.org HeLa cell cDNA library Cancer Res. (0008-5472) 2004 wang-2004f-3 A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Wang L., Bhattacharyya N., Chelsea DM., Escobar PF., Banerjee S. Cancer - Interactions investigated in the context of cancer PMID 11467963 indicates that the recombinant DNA polBeta used is of human origin and gives the range for DNA polBeta Delta. A search for MGC5306 in NCBI Entrez found CAG33650 containing a reference to the TrEMBL entry Q6I9Y6, which corresponds to primary UniProtKB accession number Q9H5J8. banerjs@ccf.org HeLa cell cDNA library Cancer Res. (0008-5472) 2004 dpolb_human DNA polymerase beta POLB protein protein human Homo sapiens Human MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE taf1d_human TATA box-binding protein-associated factor RNA polymerase I subunit D RNA polymerase I-specific TBP-associated factor 41 kDa TAF1D JOSD3 TATA box-binding protein-associated factor 1D Transcription initiation factor SL1/TIF-IB subunit D protein protein human Homo sapiens Human MDKSGIDSLDHVTSDAVELANRSDNSSDSSLFKTQCIPYSPKGEKRNPIRKFVRTPESVHASDSSSDSSFEPIPLTIKAIFERFKNRKKRYKKKKKRRYQPTGRPRGRPEGRRNPIYSLIDKKKQFRSRGSGFPFLESENEKNAPWRKILTFEQAVARGFFNYIEKLKYEHHLKESLKQMNVGEDLENEDFDSRRYKFLDDDGSISPIEESTAEDEDATHLEDNECDIKLAGDSFIVSSEFPVRLSVYLEEEDITEEAALSKKRATKAKNTGQRGLKM xrcc1_human DNA repair protein XRCC1 XRCC1 X-ray repair cross-complementing protein 1 protein protein human Homo sapiens Human MPEIRLRHVVSCSSQDSTHCAENLLKADTYRKWRAAKAGEKTISVVLQLEKEEQIHSVDIGNDGSAFVEVLVGSSAGGAGEQDYEVLLVTSSFMSPSESRSGSNPNRVRMFGPDKLVRAAAEKRWDRVKIVCSQPYSKDSPFGLSFVRFHSPPDKDEAEAPSQKVTVTKLGQFRVKEEDESANSLRPGALFFSRINKTSPVTASDPAGPSYAAATLQASSAASSASPVSRAIGSTSKPQESPKGKRKLDLNQEEKKTPSKPPAQLSPSVPKRPKLPAPTRTPATAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSRRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDNMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA polb-josd3 DNA polymerase beta Delta interacts with MGC5306 in a yeast two-hybrid assay 670426 N/A 670429 unspecified role unspecified role bait bait region DNA polBeta Delta sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 670430 unspecified role unspecified role prey prey physical association physical association false false false 1 A polb-xrcc1 DNA polymerase beta Delta interacts with XRCC1 in a yeast two-hybrid assay 670426 N/A 670429 unspecified role unspecified role bait bait region DNA polBeta Delta sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 670431 unspecified role unspecified role prey prey physical association physical association false false false 1 A josd3-polb 5306-GFP coimmunoprecipitates endogenous DNA polBeta from 293 lysates 670427 N/A 670429 unspecified role unspecified role prey prey N/A 670430 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 B Q9H5J8 transfection josd3-polb-1 5306-GFP coimmunoprecipitates exogenous DNA polBeta Delta from 293 lysates 670427 N/A 670430 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 670429 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 B Q9H5J8; P06746 transfection polb-josd3-1 DNA polBeta and MGC5306 colocalize in nuclei of HEK293 cells 670428 N/A 670429 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 670430 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2 B Colocalization occurs in the nuclei of HEK293 cells.