IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ikeda-2004-3 Protein phosphatase 5 is a negative regulator of estrogen receptor-mediated transcription. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ikeda K., Ogawa S., Tsukui T., Horie-Inoue K., Ouchi Y., Kato S., Muramatsu M., Inoue S. Cancer - Interactions investigated in the context of cancer INOUE-GER@h.u-tokyo.ac.jp A cDNA library derived from estrogen-depleted MCF7 cells was used. Mol. Endocrinol. (0888-8809) 2004 Only protein-protein interactions imex curation ikeda-2004-4 Protein phosphatase 5 is a negative regulator of estrogen receptor-mediated transcription. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ikeda K., Ogawa S., Tsukui T., Horie-Inoue K., Ouchi Y., Kato S., Muramatsu M., Inoue S. Cancer - Interactions investigated in the context of cancer INOUE-GER@h.u-tokyo.ac.jp A cDNA library derived from estrogen-depleted MCF7 cells was used. Mol. Endocrinol. (0888-8809) 2004 Only protein-protein interactions imex curation ikeda-2004-1 Protein phosphatase 5 is a negative regulator of estrogen receptor-mediated transcription. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ikeda K., Ogawa S., Tsukui T., Horie-Inoue K., Ouchi Y., Kato S., Muramatsu M., Inoue S. Cancer - Interactions investigated in the context of cancer INOUE-GER@h.u-tokyo.ac.jp A cDNA library derived from estrogen-depleted MCF7 cells was used. Mol. Endocrinol. (0888-8809) 2004 Only protein-protein interactions imex curation ikeda-2004-2 Protein phosphatase 5 is a negative regulator of estrogen receptor-mediated transcription. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ikeda K., Ogawa S., Tsukui T., Horie-Inoue K., Ouchi Y., Kato S., Muramatsu M., Inoue S. Cancer - Interactions investigated in the context of cancer INOUE-GER@h.u-tokyo.ac.jp A cDNA library derived from estrogen-depleted MCF7 cells was used. Mol. Endocrinol. (0888-8809) 2004 Only protein-protein interactions imex curation ikeda-2004-5 Protein phosphatase 5 is a negative regulator of estrogen receptor-mediated transcription. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ikeda K., Ogawa S., Tsukui T., Horie-Inoue K., Ouchi Y., Kato S., Muramatsu M., Inoue S. Cancer - Interactions investigated in the context of cancer INOUE-GER@h.u-tokyo.ac.jp A cDNA library derived from estrogen-depleted MCF7 cells was used. Mol. Endocrinol. (0888-8809) 2004 Only protein-protein interactions imex curation ikeda-2004-6 Protein phosphatase 5 is a negative regulator of estrogen receptor-mediated transcription. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ikeda K., Ogawa S., Tsukui T., Horie-Inoue K., Ouchi Y., Kato S., Muramatsu M., Inoue S. Cancer - Interactions investigated in the context of cancer INOUE-GER@h.u-tokyo.ac.jp A cDNA library derived from estrogen-depleted MCF7 cells was used. Mol. Endocrinol. (0888-8809) 2004 Only protein-protein interactions imex curation ppp5_human Serine/threonine-protein phosphatase 5 Protein phosphatase T PPP5C PPP5 protein protein human Homo sapiens Human MAMAEGERTECAEPPRDEPPADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRSLAYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQECNKIVKQKAFERAIAGDEHKRSVVDSLDIESMTIEDEYSGPKLEDGKVTISFMKELMQWYKDQKKLHRKCAYQILVQVKEVLSKLSTLVETTLKETEKITVCGDTHGQFYDLLNIFELNGLPSETNPYIFNGDFVDRGSFSVEVILTLFGFKLLYPDHFHLLRGNHETDNMNQIYGFEGEVKAKYTAQMYELFSEVFEWLPLAQCINGKVLIMHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLLWSDPQPQNGRSISKRGVSCQFGPDVTKAFLEENNLDYIIRSHEVKAEGYEVAHGGRCVTVFSAPNYCDQMGNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMAYANTLLQLGMM esr2_human Estrogen receptor beta ESR2 ESTRB NR3A2 Nuclear receptor subfamily 3 group A member 2 protein protein human Homo sapiens Human MDIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQLVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKLADKELVHMISWAKKIPGFVELSLFDQVRLLESCWMEVLMMGLMWRSIDHPGKLIFAPDLVLDRDEGKCVEGILEIFDMLLATTSRFRELKLQHKEYLCVKAMILLNSSMYPLVTATQDADSSRKLAHLLNAVTDALVWVIAKSGISSQQQSMRLANLLMLLSHVRHASNKGMEHLLNMKCKNVVPVYDLLLEMLNAHVLRGCKSSITGSECSPAEDSKSKEGSQNPQSQ esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV ppp5c-esr2 Estrogen receptor beta is pulled down by protein phosphatase 5 in vitro 740306 N/A 740312 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 740313 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false 2 This interaction is not ligand-dependent. The level of interaction was not affected by the presence or absence of E2. ppp5c-esr1 Estrogen receptor alpha is pulled down by protein phosphatase 5 in vitro 740306 N/A 740314 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 740312 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 2 This interaction is not ligand-dependent. The level of interaction was not affected by the presence or absence of E2. esr2-ppp5c-2 Estrogen receptor beta interacts with protein phosphatase 5 in a directed mammalian two-hybrid assay 740307 N/A 740313 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 740312 unspecified role unspecified role prey prey physical association physical association false false false 3 The highest level of interaction was observed when the ERbeta construct containing only the E domain (aa 248-481) was used. esr1-ppp5c Estrogen receptor alpha interacts with protein phosphatase 5 in a directed mammalian two-hybrid assay 740307 N/A 740312 unspecified role unspecified role prey prey N/A 740314 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3 The highest levels of this interaction were observed when the ERalpha construct containing only the E domain (aa 302-530) was used. esr2-ppp5c Estrogen receptor beta interacts with protein phosphatase 5 in a yeast two-hybrid assay 740308 N/A 740312 unspecified role unspecified role prey prey N/A 740313 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The Estrogen receptor (ER) beta construct aa 1-481 is a dominant negative form, that has the potential to repress both ERalpha- and ERbeta-mediated transactivation. esr2-ppp5c-1 Estrogen receptor beta interacts with protein phosphatase 5 in a directed yeast two-hybrid assay 740309 N/A 740312 unspecified role unspecified role prey prey N/A 740313 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The Estrogen receptor (ER) beta construct aa 1-481 is a dominant negative form, that has the potential to repress both ERalpha- and ERbeta-mediated transactivation. 1 ppp5c-esr1-1 Estrogen receptor alpha is coimmunoprecipitated with protein phosphatase 5 in 293T lysates 740310 N/A 740312 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 740314 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4 B ppp5c-esr1-2 Endogenous ER alpha coimmunoprecipitates with endogenous protein phosphatase 5 in MCF7 lysates 740311 N/A 740314 unspecified role unspecified role prey prey N/A 740312 unspecified role unspecified role bait bait physical association physical association false false false 4 C