IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kurki-2003-1 Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. in vitro In vitro coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis 3 marikki.laiho@helsinki.fi J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer kurki-2003-4 Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Human fibroblasts (WS1) expressing wild type p53 were treated with UVC to induce DNA damage. 3 marikki.laiho@helsinki.fi J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer kurki-2003-5 Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis 3 marikki.laiho@helsinki.fi J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer kurki-2003-8 Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. imaging technique imaging technique microscopy immunostaining immunostaining Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 and mdm2 negative cells were transfected. Fluorescence microscopy was used for visualisation. 3 marikki.laiho@helsinki.fi J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer kurki-2003-2 Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Human SaOS-2 cell treated with proteasomal inhibitor MG132. 3 marikki.laiho@helsinki.fi J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer kurki-2003-3 Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Human SaOS-2 cell treated with UV radiation. 3 marikki.laiho@helsinki.fi J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer kurki-2003-6 Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma imaging technique imaging technique microscopy immunostaining immunostaining Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Colocalisation was found after treatments with UV, proteasomal inhibitor MG132 and arsenic trioxide. Fluorescence microscopy was used to visualise. 3 marikki.laiho@helsinki.fi J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer kurki-2003-7 Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. imaging technique imaging technique microscopy immunostaining immunostaining Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Colocalisation was found after treatments with UVC. Confocal microscopy was used to visualise. 3 marikki.laiho@helsinki.fi J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer pml_human Protein PML PML MYL RNF71 TRIM19 Tripartite motif-containing protein 19 RING finger protein 71 Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP p29590-5 Protein PML PML MYL RNF71 TRIM19 RING finger protein 71 Tripartite motif-containing protein 19 PML-IV PML-X TRIM19zeta Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNESGFSWGYPHPFLI p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD mdm2-pml-1 Interaction between human Mdm2 and human PML isoform detected by coIP 303084 n/a 303092 unspecified role unspecified role prey prey in vitro In vitro N/A 303093 unspecified role unspecified role bait bait in vitro In vitro direct interaction direct interaction false false false mdm2-pmlx-1 Interaction between human Mdm2 and human PML-X detected by coIP 303084 N/A 303094 unspecified role unspecified role prey prey in vitro In vitro N/A 303093 unspecified role unspecified role bait bait in vitro In vitro direct interaction direct interaction false false false mdm2-pmlx-2 Interaction between human Mdm2 and human PML-X detected by coIP 303084 N/A 303093 unspecified role unspecified role bait bait del58-89 Mdm2 amino acid deletion 58-89 sufficient to bind sufficient binding region sufficient to bind less-than less-than greater-than greater-than false in vitro In vitro N/A 303094 unspecified role unspecified role prey prey in vitro In vitro direct interaction direct interaction false false false mdm2-pmlx-3 Interaction between human Mdm2 and human PML-X detected by coIP 303084 N/A 303094 unspecified role unspecified role prey prey in vitro In vitro N/A 303093 unspecified role unspecified role bait bait del89-222 Mdm2 amino acid deletion 89-222 sufficient to bind sufficient binding region sufficient to bind less-than less-than greater-than greater-than false in vitro In vitro direct interaction direct interaction false false false mdm2-pmlx-4 Interaction between human Mdm2 and human PML-X detected by coIP 303084 N/A 303093 unspecified role unspecified role bait bait del222-437 Mdm2 amino acid deletion 222-437 sufficient to bind sufficient binding region sufficient to bind less-than less-than greater-than greater-than false in vitro In vitro N/A 303094 unspecified role unspecified role prey prey in vitro In vitro direct interaction direct interaction false false false p53-mdm2-1 Interaction between human p53 and human Mdm2 detected by coIP 303085 N/A 303093 unspecified role unspecified role prey prey human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. N/A 303095 unspecified role unspecified role bait bait human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. physical association physical association false false false p53-pml-1 Interaction between human p53 and human PML detected by coIP 303085 N/A 303095 unspecified role unspecified role bait bait human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. N/A 303092 unspecified role unspecified role prey prey human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. physical association physical association false false false p53-pmlx-mdm2-1 Complex formation between human proteins p53, PML-X and Mdm2 detected by coIP 303086 N/A 303094 unspecified role unspecified role prey prey in vitro In vitro N/A 303095 unspecified role unspecified role bait bait in vitro In vitro N/A 303093 unspecified role unspecified role prey prey in vitro In vitro association association false false false p53-pmlx-mdm2-2 Complex formation between human proteins p53, PML-X and Mdm2 detected by coIP 303086 N/A 303094 unspecified role unspecified role prey prey in vitro In vitro N/A 303095 unspecified role unspecified role prey prey in vitro In vitro N/A 303093 unspecified role unspecified role bait bait in vitro In vitro association association false false false pml-mdm2-3 Interaction between human Mdm2 and human PML III isoform detected by colocalisation 303087 n/a 303092 unspecified role unspecified role neutral component neutral component in vitro In vitro N/A 303093 unspecified role unspecified role neutral component neutral component in vitro In vitro direct interaction direct interaction false false false pml-mdm2-4 Interaction between human Mdm2 and human PML-X detected by colocalisation 303087 N/A 303094 unspecified role unspecified role neutral component neutral component in vitro In vitro N/A 303093 unspecified role unspecified role neutral component neutral component in vitro In vitro direct interaction direct interaction false false false pml-mdm2-1 Interaction between human PML and human Mdm2 detected by coIP 303088 N/A 303093 unspecified role unspecified role bait bait human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma N/A 303092 unspecified role unspecified role prey prey human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma physical association physical association false false false pml-mdm2-2 Interaction between human PML and human Mdm2 detected by coIP 303089 N/A 303092 unspecified role unspecified role prey prey human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma N/A 303093 unspecified role unspecified role bait bait human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma physical association physical association false false false mdm2-pml-2 Interaction between human MDM2 and PML detected by colocalisation 303090 N/A 303093 unspecified role unspecified role neutral component neutral component human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma n/a 303092 unspecified role unspecified role neutral component neutral component human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma colocalization colocalization false false false mdm2-pml-3 Interaction between human MDM2 and PML detected by colocalisation 303091 N/A 303093 unspecified role unspecified role neutral component neutral component human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. n/a 303092 unspecified role unspecified role neutral component neutral component human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. colocalization colocalization false false false