IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ townson-2003-2 SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Townson SM., Dobrzycka KM., Lee AV., Air M., Deng W., Kang K., Jiang S., Kioka N., Michaelis K., Oesterreich S. Cancer - Interactions investigated in the context of cancer Source of ER taken from PMID: 9393950 as described in the text steffio@breastcenter.tmc.edu J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation townson-2003-1 SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Townson SM., Dobrzycka KM., Lee AV., Air M., Deng W., Kang K., Jiang S., Kioka N., Michaelis K., Oesterreich S. Cancer - Interactions investigated in the context of cancer Source of ER taken from PMID: 9393950 as described in the text steffio@breastcenter.tmc.edu J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation townson-2003-3 SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Townson SM., Dobrzycka KM., Lee AV., Air M., Deng W., Kang K., Jiang S., Kioka N., Michaelis K., Oesterreich S. Cancer - Interactions investigated in the context of cancer Source of ER taken from PMID: 9393950 as described in the text steffio@breastcenter.tmc.edu J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation townson-2003-4 SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor. chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Townson SM., Dobrzycka KM., Lee AV., Air M., Deng W., Kang K., Jiang S., Kioka N., Michaelis K., Oesterreich S. Cancer - Interactions investigated in the context of cancer Source of ER taken from PMID: 9393950 as described in the text steffio@breastcenter.tmc.edu J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation townson-2003-5 SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Townson SM., Dobrzycka KM., Lee AV., Air M., Deng W., Kang K., Jiang S., Kioka N., Michaelis K., Oesterreich S. Cancer - Interactions investigated in the context of cancer Source of ER taken from PMID: 9393950 as described in the text steffio@breastcenter.tmc.edu J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation townson-2003-6 SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor. human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma confocal microscopy confocal microscopy immunostaining immunostaining Townson SM., Dobrzycka KM., Lee AV., Air M., Deng W., Kang K., Jiang S., Kioka N., Michaelis K., Oesterreich S. Cancer - Interactions investigated in the context of cancer Fluorescently tagged proteins detected by direct fluorescence. Source of ER taken from PMID: 9393950 as described in the text steffio@breastcenter.tmc.edu J. Biol. Chem. (0021-9258) 2003 Only protein-protein interactions imex curation safb2_human Scaffold attachment factor B2 SAFB2 KIAA0138 protein protein human Homo sapiens Human MAETLPGSGDSGPGTASLGPGVAETGTRRLSELRVIDLRAELKKRNLDTGGNKSVLMERLKKAVKEEGQDPDEIGIELEATSKKSAKRCVKGLKMEEEGTEDNGLEDDSRDGQEDMEASLENLQNMGMMDMSVLDETEVANSSAPDFGEDGTDGLLDSFCDSKEYVAAQLRQLPAQPPEHAVDGEGFKNTLETSSLNFKVTPDIEESLLEPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTAHAQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARDSKEDGRKFDFDACNEVPPAPKESSTSEGADQKMSSFKEEKDIKPIIKDEKGRVGSGSGRNLWVSGLSSTTRATDLKNLFSKYGKVVGAKVVTNARSPGARCYGFVTMSTSDEATKCISHLHRTELHGRMISVEKAKNEPAGKKLSDRKECEVKKEKLSSVDRHHSVEIKIEKTVIKKEEKIEKKEEKKPEDIKKEEKDQDELKPGPTNRSRVTKSGSRGMERTVVMDKSKGEPVISVKTTSRSKERSSKSQDRKSESKEKRDILSFDKIKEQRERERQRQREREIRETERRREREQREREQRLEAFHERKEKARLQRERLQLECQRQRLERERMERERLERERMRVERERRKEQERIHREREELRRQQEQLRYEQERRPGRRPYDLDRRDDAYWPEGKRVAMEDRYRADFPRPDHRFHDFDHRDRGQYQDHAIDRREGSRPMMGDHRDGQHYGDDRHGHGGPPERHGRDSRDGWGGYGSDKRLSEGRGLPPPPRGGRDWGEHNQRLEEHQARAWQGAMDAGAASREHARWQGGERGLSGPSGPGHMASRGGVAGRGGFAQGGHSQGHVVPGGGLEGGGVASQDRGSRVPHPHPHPPPYPHFTRRY esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV o60504-2 Vinexin SORBS3 SCAM1 SH3-containing adapter molecule 1 Sorbin and SH3 domain-containing protein 3 protein protein human Homo sapiens Human MADGGSPFLGRRDFVYPSSTRDPSASNGGGSPARREEKKRKAARLKFDFQAQSPKELTLQKGDIVYIHKEVDKNWLEGEHHGRLGIFPANYVEVLPADEIPKPIKPPTYQVLEYGEAVAQYTFKGDLEVELSFRKGEHICLIRKVNENWYEGRITGTGRQGIFPASYVQVSREPRLRLCDDGPQLPTSPRLTAAARSARHPSSPSALRSPADPIDLGGQTSPRRTGFSFPTQEPRPQTQNLGTPGPALSHSRGPSHPLDLGTSSPNTSQIHWTPYRAMYQYRPQNEDELELREGDRVDVMQQCDDGWFVGVSRRTQKFGTFPGNYVAPV Contains a phosphoserine at position 6. Initiator Met-1 is removed. Contains a N-acetylalanine at position 2. o60504-1 Vinexin SORBS3 SCAM1 SH3-containing adapter molecule 1 Sorbin and SH3 domain-containing protein 3 protein protein human Homo sapiens Human MQGPPRSLRAGLSLDDFIPGHLQSHIGSSSRGTRVPVIRNGGSNTLNFQFHDPAPRTVCNGGYTPRRDASQHPDPAWYQTWPGPGSKPSASTKIPASQHTQNWSATWTKDSKRRDKRWVKYEGIGPVDESGMPIAPRSSVDRPRDWYRRMFQQIHRKMPDLQLDWTFEEPPRDPRHLGAQQRPAHRPGPATSSSGRSWDHSEELPRSTFNYRPGAFSTVLQPSNQVLRRREKVDNVWTEESWNQFLQELETGQRPKKPLVDDPGEKPSQPIEVLLERELAELSAELDKDLRAIETRLPSPKSSPAPRRAPEQRPPAGPASAWSSSYPHAPYLGSARSLSPHKMADGGSPFLGRRDFVYPSSTRDPSASNGGGSPARREEKKRKAARLKFDFQAQSPKELTLQKGDIVYIHKEVDKNWLEGEHHGRLGIFPANYVEVLPADEIPKPIKPPTYQVLEYGEAVAQYTFKGDLEVELSFRKGEHICLIRKVNENWYEGRITGTGRQGIFPASYVQVSREPRLRLCDDGPQLPTSPRLTAAARSARHPSSPSALRSPADPIDLGGQTSPRRTGFSFPTQEPRPQTQNLGTPGPALSHSRGPSHPLDLGTSSPNTSQIHWTPYRAMYQYRPQNEDELELREGDRVDVMQQCDDGWFVGVSRRTQKFGTFPGNYVAPV safb1_human Scaffold attachment factor B1 SAFB HAP HET SAFB1 HSP27 estrogen response element-TATA box-binding protein protein protein human Homo sapiens Human MAETLSGLGDSGAAGAAALSSASSETGTRRLSDLRVIDLRAELRKRNVDSSGNKSVLMERLKKAIEDEGGNPDEIEITSEGNKKTSKRSSKGRKPEEEGVEDNGLEENSGDGQEDVETSLENLQDIDIMDISVLDEAEIDNGSVADCVEDDDADNLQESLSDSRELVEGEMKELPEQLQEHAIEDKETINNLDTSSSDFTILQEIEEPSLEPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTAHAQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARDSKEDGRKFDFDACNEVPPAPKESSTSEGADQKMSSPEDDSDTKRLSKEEKGRSSCGRNFWVSGLSSTTRATDLKNLFSKYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCINHLHKTELHGKMISVEKAKNEPVGKKTSDKRDSDGKKEKSSNSDRSTNLKRDDKCDRKDDAKKGDDGSGEKSKDQDDQKPGPSERSRATKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKEPRKSRDSESHSRVRERSEREQRMQAQWEREERERLEIARERLAFQRQRLERERMERERLERERMHVEHERRREQERIHREREELRRQQELRYEQERRPAVRRPYDLDRRDDAYWPEAKRAALDERYHSDFNRQDRFHDFDHRDRGRYPDHSVDRREGSRSMMGEREGQHYPERHGGPERHGRDSRDGWGGYGSDKRMSEGRGLPPPPRRDWGDHGRREDDRSWQGTADGGMMDRDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHPIPHGGMQGGFGGQSRGSRPSDARFTRRY safb2-esr1-2 Interaction between SAFB2 and ESR1 identified by coimmunopreciptiation 793328 n/a 793334 unspecified role unspecified role bait bait in vitro In vitro n/a 793335 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3C safb2-sorbs3-1 Interaction between SAFB2 and SORBS3 (Vinexin) identified by coimmunopreciptiation 793328 n/a 793336 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 793334 unspecified role unspecified role bait bait in vitro In vitro physical association physical association false false false Fig. 5B safb2-sorbs3-3 Interaction between SAFB2 and SORBS3 (Vinexin) identified by coimmunopreciptiation 793328 n/a 793337 unspecified role unspecified role prey prey n/a 793336 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 793334 unspecified role unspecified role bait bait in vitro In vitro association association false false false Fig. 5D safb2-esr1-1 Interaction between SAFB2 and ESR1 identified by pulldown 793329 n/a 793335 unspecified role unspecified role bait bait dbd_hinge region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 793334 unspecified role unspecified role prey prey in vitro In vitro direct interaction direct interaction false false false Fig. 3A safb1-safb2-1 793330 n/a 793334 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 793338 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 793360 793358 physical association physical association false false false Fig. 4A Amino acids 719-808 are the major interaction region and amino acids 599-720 are a region of minor interaction. safb1-safb2-2 Interaction between SAFB1 and SAFB2 identified by coimmunopreciptiation 793331 n/a 793338 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 793334 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4B Amino acids 719-808 are the major interaction region and amino acids 519-720 are a region of minor interaction. safb1-safb2-3 Interaction between SAFB1 and SAFB2 identified by coimmunopreciptiation 793332 n/a 793334 unspecified role unspecified role prey prey n/a 793338 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4C safb2-sorbs3-2 Colocalisation of SAFB2 and SORBS3 (Vinexin) 793333 n/a 793336 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 793334 unspecified role unspecified role neutral component neutral component in vitro In vitro colocalization colocalization false false false Fig. 5C