IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ caballero-2002-3 Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Caballero OL., Resto V., Patturajan M., Meerzaman D., Guo MZ., Engles J., Yochem R., Ratovitski E., Sidransky D., Jen J. Cancer - Interactions investigated in the context of cancer jenj@mail.nih.gov A human fetal brain Matchmaker cDNA library (Clontech) was used. Oncogene (0950-9232) 2002 caballero-2002-1 Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Caballero OL., Resto V., Patturajan M., Meerzaman D., Guo MZ., Engles J., Yochem R., Ratovitski E., Sidransky D., Jen J. Cancer - Interactions investigated in the context of cancer jenj@mail.nih.gov A human fetal brain Matchmaker cDNA library (Clontech) was used. Oncogene (0950-9232) 2002 caballero-2002-2 Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Caballero OL., Resto V., Patturajan M., Meerzaman D., Guo MZ., Engles J., Yochem R., Ratovitski E., Sidransky D., Jen J. Cancer - Interactions investigated in the context of cancer jenj@mail.nih.gov A human fetal brain Matchmaker cDNA library (Clontech) was used. Oncogene (0950-9232) 2002 uchl1_human Ubiquitin carboxyl-terminal hydrolase isozyme L1 Ubiquitin thioesterase L1 Neuron cytoplasmic protein 9.5 PGP 9.5 UCHL1 protein protein human Homo sapiens Human MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA csn5_human COP9 signalosome complex subunit 5 Jun activation domain-binding protein 1 COPS5 CSN5 JAB1 protein protein human Homo sapiens Human MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMSQVIKDKLFNQINIS fbrl_human rRNA 2'-O-methyltransferase fibrillarin FBL FIB1 FLRN Histone-glutamine methyltransferase 34 kDa nucleolar scleroderma antigen U6 snRNA 2'-O-methyltransferase fibrillarin protein protein human Homo sapiens Human MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS ranb9_human Ran-binding protein 9 RanBP7 Ran-binding protein M BPM90 BPM-L RANBP9 RANBPM protein protein human Homo sapiens Human MSGQPPPPPPQQQQQQQQLSPPPPAALAPVSGVVLPAPPAVSAGSSPAGSPGGGAGGEGLGAAAAALLLHPPPPPPPATAAPPPPPPPPPPPASAAAPASGPPAPPGLAAGPGPAGGAPTPALVAGSSAAAPFPHGDSALNEQEKELQRRLKRLYPAVDEQETPLPRSWSPKDKFSYIGLSQNNLRVHYKGHGKTPKDAASVRATHPIPAACGIYYFEVKIVSKGRDGYMGIGLSAQGVNMNRLPGWDKHSYGYHGDDGHSFCSSGTGQPYGPTFTTGDVIGCCVNLINNTCFYTKNGHSLGIAFTDLPPNLYPTVGLQTPGEVVDANFGQHPFVFDIEDYMREWRTKIQAQIDRFPIGDREGEWQTMIQKMVSSYLVHHGYCATAEAFARSTDQTVLEELASIKNRQRIQKLVLAGRMGEAIETTQQLYPSLLERNPNLLFTLKVRQFIEMVNGTDSEVRCLGGRSPKSQDSYPVSPRPFSSPSMSPSHGMNIHNLASGKGSTAHFSGFESCSNGVISNKAHQSYCHSNKHQSSNLNVPELNSINMSRSQQVNNFTSNDVDMETDHYSNGVGETSSNGFLNGSSKHDHEMEDCDTEMEVDSSQLRRQLCGGSQAAIERMIHFGRELQAMSEQLRRDCGKNTANKKMLKDAFSLLAYSDPWNSPVGNQLDPIQREPVCSALNSAILETHNLPKQPPLALAMGQATQCLGLMARSGIGSCAFATVEDYLH cops5-uchl1-1 PGP9.5 colocalizes with JAB1 in H1299 cells 575332 N/A 575335 unspecified role unspecified role neutral component neutral component N/A 575336 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1 B cops5-fbl JAB1 colocalizes with fibrillarin in H1299 cells 575332 N/A 575336 unspecified role unspecified role neutral component neutral component N/A 575337 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2 Fibrillarin is only present in the nucleolus in interphase cells. cdn1b-cops5 p27 colocalizes with JAB1 in H1299 cells 575332 N/A 575336 unspecified role unspecified role neutral component neutral component N/A 575338 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2 cdn1b-uchl1 p27 colocalizes with PGP9.5 in H1299 cells 575332 N/A 575338 unspecified role unspecified role neutral component neutral component N/A 575335 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2 uchl1-cops5 PGP9.5 interacts with JAB1 in a yeast two-hybrid assay 575333 N/A 575335 unspecified role unspecified role bait bait N/A 575336 unspecified role unspecified role prey prey physical association physical association false false false uchl1-ube2i PGP9.5 interacts with UBC9 in a yeast two-hybrid assay 575333 N/A 575339 unspecified role unspecified role prey prey N/A 575335 unspecified role unspecified role bait bait physical association physical association false false false uchl1-ranbp9 PGP9.5 interacts with RanBPM in a yeast two-hybrid assay 575333 N/A 575335 unspecified role unspecified role bait bait N/A 575340 unspecified role unspecified role prey prey physical association physical association false false false cops5-uchl1 PGP9.5 coimmunoprecipitates with JAB1 in H1299 cell lysates 575334 N/A 575335 unspecified role unspecified role prey prey N/A 575336 unspecified role unspecified role bait bait physical association physical association false false false 1 A cops5-cdkn1b p27 coimmunoprecipitates with JAB1 in H1299 cell lysates 575334 N/A 575336 unspecified role unspecified role bait bait N/A 575338 unspecified role unspecified role prey prey physical association physical association false false false 3 A cdkn1b-uchl1 PGP9.5 coimmunoprecipitates with p27 in H1299 cell lysates 575334 N/A 575338 unspecified role unspecified role bait bait N/A 575335 unspecified role unspecified role prey prey physical association physical association false false false 3 C The antibody used, K25020, mainly recognizes the nuclear form of p27. The M197 antibody which recognizes mainly the cytoplasmic form of p27 did not coimmunoprecipitate PGP9.5.