IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sakai-2002-1 Interaction of a paraneoplastic cerebellar degeneration-associated neuronal protein with the nuclear helix-loop-helix leucine zipper protein MRG X. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Sakai K., Shirakawa T., Li Y., Kitagawa Y., Hirose G. Cancer - Interactions investigated in the context of cancer The sequences in Fig. 1 were compared to the exon sequence in the VEGA database in order to determine that the AS9 clone coded for amino acids 161-288 of full length MRG X. ksakai@kanazawa-med.ac.jp A human brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2002 Only protein-protein interactions imex curation sakai-2002-2 Interaction of a paraneoplastic cerebellar degeneration-associated neuronal protein with the nuclear helix-loop-helix leucine zipper protein MRG X. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sakai K., Shirakawa T., Li Y., Kitagawa Y., Hirose G. Cancer - Interactions investigated in the context of cancer The sequences in Fig. 1 were compared to the exon sequence in the VEGA database in order to determine that the AS9 clone coded for amino acids 161-288 of full length MRG X. ksakai@kanazawa-med.ac.jp A human brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2002 Only protein-protein interactions imex curation sakai-2002-3 Interaction of a paraneoplastic cerebellar degeneration-associated neuronal protein with the nuclear helix-loop-helix leucine zipper protein MRG X. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Sakai K., Shirakawa T., Li Y., Kitagawa Y., Hirose G. Cancer - Interactions investigated in the context of cancer GST-PCD17 protein was blotted onto a PVDF membrane and probed with the FLAG-tagged purified C-terminal region of MRG X, followed by Western blotting with an anti-FLAG M2 antibody. The sequences in Fig. 1 were compared to the exon sequence in the VEGA database in order to determine that the AS9 clone coded for amino acids 161-288 of full length MRG X. ksakai@kanazawa-med.ac.jp A human brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2002 Only protein-protein interactions imex curation sakai-2002-4 Interaction of a paraneoplastic cerebellar degeneration-associated neuronal protein with the nuclear helix-loop-helix leucine zipper protein MRG X. human-t98g Human caucasian glioblastoma line T98G t98g Human Caucasian glioblastoma T98G fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Sakai K., Shirakawa T., Li Y., Kitagawa Y., Hirose G. Cancer - Interactions investigated in the context of cancer The sequences in Fig. 1 were compared to the exon sequence in the VEGA database in order to determine that the AS9 clone coded for amino acids 161-288 of full length MRG X. ksakai@kanazawa-med.ac.jp A human brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2002 Only protein-protein interactions imex curation sakai-2002-5 Interaction of a paraneoplastic cerebellar degeneration-associated neuronal protein with the nuclear helix-loop-helix leucine zipper protein MRG X. human-t98g Human caucasian glioblastoma line T98G t98g Human Caucasian glioblastoma T98G anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sakai K., Shirakawa T., Li Y., Kitagawa Y., Hirose G. Cancer - Interactions investigated in the context of cancer Cell lysates of cells transiently transfected with PDC17 and FLAG-tagged MRG X were prepared, followed by immunoprecipitation with anti-FLAG antibody affinity resin and immunoblotting with anti-Purkinje cell antibodies (anti-Yo). The sequences in Fig. 1 were compared to the exon sequence in the VEGA database in order to determine that the AS9 clone coded for amino acids 161-288 of full length MRG X. ksakai@kanazawa-med.ac.jp A human brain cDNA library was used. Mol. Cell. Neurosci. (1044-7431) 2002 Only protein-protein interactions imex curation mo4l2_human Mortality factor 4-like protein 2 MORF4L2 KIAA0026 MRGX MORF-related gene X protein Transcription factor-like protein MRGX Protein MSL3-2 protein protein human Homo sapiens Human MSSRKQGSQPRGQQSAEEENFKKPTRSNMQRSKMRGASSGKKTAGPQQKNLEPALPGRWGGRSAENPPSGSVRKTRKNKQKTPGNGDGGSTSEAPQPPRKKRARADPTVESEEAFKNRMEVKVKIPEELKPWLVEDWDLVTRQKQLFQLPAKKNVDAILEEYANCKKSQGNVDNKEYAVNEVVAGIKEYFNVMLGTQLLYKFERPQYAEILLAHPDAPMSQVYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLGYLHDFLKYLAKNSASLFTASDYKVASAEYHRKAL cdr2_human Cerebellar degeneration-related protein 2 Paraneoplastic cerebellar degeneration-associated antigen Major Yo paraneoplastic antigen CDR2 PCD17 protein protein human Homo sapiens Human MLAENLVEEFEMKEDEPWYDHQDLQQDLQLAAELGKTLLDRNTELEDSVQQMYTTNQEQLQEIEYLTKQVELLRQMNEQHAKVYEQLDVTARELEETNQKLVADSKASQQKILSLTETIECLQTNIDHLQSQVEELKSSGQGRRSPGKCDQEKPAPSFACLKELYDLRQHFVYDHVFAEKITSLQGQPSPDEEENEHLKKTVTMLQAQLSLERQKRVTMEEEYGLVLKENSELEQQLGATGAYRARALELEAEVAEMRQMLQSEHPFVNGVEKLVPDSLYVPFKEPSQSLLEEMFLTVPESHRKPLKRSSSETILSSLAGSDIVKGHEETCIRRAKAVKQRGISLLHEVDTQYSALKVKYEELLKKCQEEQDSLSHKAVQTSRAAAKDLTGVNAQSEPVASGWELASVNPEPVSSPTTPPEYKALFKEIFSCIKKTKQEIDEQRTKYRSLSSHS o14596_human protein protein human Homo sapiens Human MLPAATEVQHRLQGQKDMVWKWMPLLLLLVCVATMCSAQDRTDLLNVCMDAKHHKTKPGPEDKLHDQCSPWKKNACCTASTSQELHKDTSRLYNFNWDHCGKMEPACSATSSRTPVSMSAHQPGALDPAGESELAAKNASWMCPYAKSTVSAGGRIVTPPTRARATGTEDGTGPQELTSAQLGLSAAPLSPTSPLQLPFVKASGVTHTRSATTAEGAAAASRCGLLQPRATPTRKWRGSMLQPCM assy_human Argininosuccinate synthase Citrulline--aspartate ligase ASS1 ASS protein protein human Homo sapiens Human MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSREFVEEFIWPAIQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQVRFELSCYSLAPQIKVIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDILEIEFKKGVPVKVTNVKDGTTHQTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQVSVLKGQVYILGRESPLSLYNEELVSMNVQGDYEPTDATGFININSLRLKEYHRLQSKVTAK cdr2-morf4l2-2 PDC17 interacts with MRG X in a yeast two-hybrid assay 575144 N/A 575149 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 575150 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 The sequence given for MRGX in Fig. 1 shows the protein to be 288 aa (confirmed by comparison to the exon described in the VEGA database and the Uniprotkb entry)as the last codon ATG is the termination codon. However, in Fig. 2 the diagram shows MRG X as being 289 aa. The length of the CDR2 protein is given as 460 aa in Fig. 2, whereas the length of the Uniprotkb entry is 454 aa. cdr2- o14596 PDC17 interacts with folate binding protein in a yeast two-hybrid assay 575144 N/A 575151 unspecified role unspecified role prey prey N/A 575150 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The length of the CDR2 protein is given as 460 aa in Fig. 2, whereas the length of the Uniprotkb entry is 454 aa. cdr2- ass PDC17 interacts with arginosuccinate synthetase in a yeast two-hybrid assay 575144 N/A 575150 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 575152 unspecified role unspecified role prey prey physical association physical association false false false The length of the CDR2 protein is given as 460 aa in Fig. 2, whereas the length of the Uniprotkb entry is 454 aa. morf4l2-cdr2-1 PDC17 interacts with MRG X in a yeast two-hybrid assay 575145 N/A 575150 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 575149 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1, 2 The sequence given for MRGX in Fig. 1 shows the protein to be 288 aa (confirmed by comparison to the exon described in the VEGA database and the Uniprotkb entry)as the last codon ATG is the termination codon. However, in Fig. 2 the diagram shows MRG X as being 289 aa. The length of the CDR2 protein is given as 460 aa in Fig. 2, whereas the length of the Uniprotkb entry is 454 aa. morf4l2-cdr2-2 PDC17 interacts with MRG X in a yeast two-hybrid assay 575145 N/A 575150 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 575149 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1, 2 The sequence given for MRGX in Fig. 1 shows the protein to be 288 aa (confirmed by comparison to the exon described in the VEGA database and the Uniprotkb entry)as the last codon ATG is the termination codon. However, in Fig. 2 the diagram shows MRG X as being 289 aa. The length of the CDR2 protein is given as 460 aa in Fig. 2, whereas the length of the Uniprotkb entry is 454 aa. cdr2-morf4l2 PCD17 interacts with MRG X in a ligand overlay assay 575146 N/A 575149 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false N/A 575150 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false It is not clear what organism the MRG X was expressed in. 3 The sequence given for MRGX in Fig. 1 shows the protein to be 288 aa (confirmed by comparison to the exon described in the VEGA database and the Uniprotkb entry)as the last codon ATG is the termination codon. However, in Fig. 2 the diagram shows MRG X as being 289 aa. The length of the CDR2 protein is given as 460 aa in Fig. 2, whereas the length of the Uniprotkb entry is 454 aa. It is not specified which C-terminal construct of MRG X was used. cdr2-morf4l2-1 PCD17 and MRG X colocalize in T98G cells 575147 N/A 575149 unspecified role unspecified role neutral component neutral component c-terminal BFP tag fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 575150 unspecified role unspecified role neutral component neutral component c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) colocalization colocalization false false false 5 C and D The sequence given for MRGX in Fig. 1 shows the protein to be 288 aa (confirmed by comparison to the exon described in the VEGA database and the Uniprotkb entry)as the last codon ATG is the termination codon. However, in Fig. 2 the diagram shows MRG X as being 289 aa. The length of the CDR2 protein is given as 460 aa in Fig. 2, whereas the length of the Uniprotkb entry is 454 aa. Q01850; Q15014 transfection Q01850; Q15014 overexpressed morf4l2-cdr2-3 PCD17 coimmunoprecipitates with MRG X 575148 N/A 575149 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 575150 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association false false false 6 B The sequence given for MRGX in Fig. 1 shows the protein to be 288 aa (confirmed by comparison to the exon described in the VEGA database and the Uniprotkb entry)as the last codon ATG is the termination codon. However, in Fig. 2 the diagram shows MRG X as being 289 aa. The length of the CDR2 protein is given as 460 aa in Fig. 2, whereas the length of the Uniprotkb entry is 454 aa. Q01850; Q15014 transfection