IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tang-2000c-3 Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening. human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang K., Finley RL., Nie D., Honn KV. Cancer - Interactions investigated in the context of cancer k.v.honn@wayne.edu A cDNA library from human epidermoid carcinoma A431 cells. Biochemistry (0006-2960) 2000 Only protein-protein interactions imex curation tang-2000c-1 Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tang K., Finley RL., Nie D., Honn KV. Cancer - Interactions investigated in the context of cancer k.v.honn@wayne.edu A cDNA library from human epidermoid carcinoma A431 cells. Biochemistry (0006-2960) 2000 Only protein-protein interactions imex curation tang-2000c-2 Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tang K., Finley RL., Nie D., Honn KV. Cancer - Interactions investigated in the context of cancer k.v.honn@wayne.edu A cDNA library from human epidermoid carcinoma A431 cells. Biochemistry (0006-2960) 2000 Only protein-protein interactions imex curation tang-2000c-4 Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening. human-a_431 Homo sapiens epidermoid carcinoma a_431 Human epidermoid carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Tang K., Finley RL., Nie D., Honn KV. Cancer - Interactions investigated in the context of cancer k.v.honn@wayne.edu A cDNA library from human epidermoid carcinoma A431 cells. Biochemistry (0006-2960) 2000 Only protein-protein interactions imex curation k2c5_human Keratin, type II cytoskeletal 5 Cytokeratin-5 58 kDa cytokeratin KRT5 Keratin-5 Type-II keratin Kb5 protein protein human Homo sapiens Human MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGGAGSGFGFGGGAGGGFGLGGGAGFGGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPSIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDLVEDFKNKYEDEINKRTTAENEFVMLKKDVDAAYMNKVELEAKVDALMDEINFMKMFFDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS lox12_human Polyunsaturated fatty acid lipoxygenase ALOX12 Arachidonate (12S)-lipoxygenase Arachidonate (15S)-lipoxygenase Linoleate (13S)-lipoxygenase ALOX12 LOG12 Platelet-type lipoxygenase 12 12LO Lipoxin synthase 12-LO protein protein human Homo sapiens Human MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDHDVAEDLGLLQFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWVQGEDILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYCWATWKEGLPLTIAADRKDDLPPNMRFHEEKRLDFEWTLKAGALEMALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPMLLRRSTSLPSRLVLPSGMEELQAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPLVMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNSDFQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRARTQLISDGGIFDKAVSTGGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLGLPGALYAHDALRLWEIIARYVEGIVHLFYQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI lmna_human Prelamin-A/C LMNA LMN1 protein protein human Homo sapiens Human METPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRETKRRHETRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERNSNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEKEREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRGRASSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSSSGDPAEYNLRSRTVLCGTCGQPADKASASGSGAQVGGPISSGSSASSVTVTRSYRSVGGSGGGSFGDNLVTRSYLLGNSSPRTQSPQNCSIM itb4_human Integrin beta-4 GP150 ITGB4 protein protein human Homo sapiens Human MAGPRPSPWARLLLAALISVSLSGTLANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSNIVELLEEAFNRIRSNLDIRALDSPRGLRTEVTSKMFQKTRTGSFHIRRGEVGIYQVQLRALEHVDGTHVCQLPEDQKGNIHLKPSFSDGLKMDAGIICDVCTCELQKEVRSARCSFNGDFVCGQCVCSEGWSGQTCNCSTGSLSDIQPCLREGEDKPCSGRGECQCGHCVCYGEGRYEGQFCEYDNFQCPRTSGFLCNDRGRCSMGQCVCEPGWTGPSCDCPLSNATCIDSNGGICNGRGHCECGRCHCHQQSLYTDTICEINYSAIHPGLCEDLRSCVQCQAWGTGEKKGRTCEECNFKVKMVDELKRAEEVVVRCSFRDEDDDCTYSYTMEGDGAPGPNSTVLVHKKKDCPPGSFWWLIPLLLLLLPLLALLLLLCWKYCACCKACLALLPCCNRGHMVGFKEDHYMLRENLMASDHLDTPMLRSGNLKGRDVVRWKVTNNMQRPGFATHAASINPTELVPYGLSLRLARLCTENLLKPDTRECAQLRQEVEENLNEVYRQISGVHKLQQTKFRQQPNAGKKQDHTIVDTVLMAPRSAKPALLKLTEKQVEQRAFHDLKVAPGYYTLTADQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPAGTATLGRRLVNITIIKEQARDVVSFEQPEFSVSRGDQVARIPVIRRVLDGGKSQVSYRTQDGTAQGNRDYIPVEGELLFQPGEAWKELQVKLLELQEVDSLLRGRQVRRFHVQLSNPKFGAHLGQPHSTTIIIRDPDELDRSFTSQMLSSQPPPHGDLGAPQNPNAKAAGSRKIHFNWLPPSGKPMGYRVKYWIQGDSESEAHLLDSKVPSVELTNLYPYCDYEMKVCAYGAQGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYGLVNDDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPIIPDIPIVDAQSGEDYDSFLMYSDDVLRSPSGSQRPSVSDDTGCGWKFEPLLGEELDLRRVTWRLPPELIPRLSASSGRSSDAEAPHGPPDDGGAGGKGGSLPRSATPGPPGEHLVNGRMDFAFPGSTNSLHRMTTTSAAAYGTHLSPHVPHRVLSTSSTLTRDYNSLTRSEHSHSTTLPRDYSTLTSVSSHDSRLTAGVPDTPTRLVFSALGPTSLRVSWQEPRCERPLQGYSVEYQLLNGGELHRLNIPNPAQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQSPLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDIVGYLVTCEMAQGGGPATAFRVDGDSPESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQDGGPFPQLGSRAGLFQHPLQSEYSSITTTHTSATEPFLVDGLTLGAQHLEAGGSLTRHVTQEFVSRTLTTSGTLSTHMDQQFFQT trib1_human Tribbles homolog 1 SKIP1 G-protein-coupled receptor-induced gene 2 protein TRIB1 C8FW GIG2 TRB1 protein protein human Homo sapiens Human MRVGPVRSAMSGASQPRGPALLFPATRGVPAKRLLDADDAAAVAAKCPRLSECSSPPDYLSPPGSPCSPQPPPAAPGAGGGSGSAPGPSRIADYLLLPLAEREHVSRALCIHTGRELRCKVFPIKHYQDKIRPYIQLPSHSNITGIVEVILGETKAYVFFEKDFGDMHSYVRSRKRLREEEAARLFKQIVSAVAHCHQSAIVLGDLKLRKFVFSTEERTQLRLESLEDTHIMKGEDDALSDKHGCPAYVSPEILNTTGTYSGKAADVWSLGVMLYTLLVGRYPFHDSDPSALFSKIRRGQFCIPEHISPKARCLIRSLLRREPSERLTAPEILLHPWFESVLEPGYIDSEIGTSDQIVPEYQEDSDISSFFC alox12-krt5-2 12-LOX coimmunoprecipitates KRT5 in A431 lysates 722807 N/A 722811 unspecified role unspecified role prey prey N/A 722812 unspecified role unspecified role bait bait physical association physical association false false false 2 B alox12-krt5-3 Exogenous 12-LOX coimmunoprecipitates KRT5 in A431 lysates 722807 N/A 722812 unspecified role unspecified role bait bait N/A 722811 unspecified role unspecified role prey prey physical association physical association false false false 2 B P18054 tansfection P18054 over-expression krt5-alox12 12-LOX coimmunoprecipitates with KRT5 in A431 lysates 722807 N/A 722811 unspecified role unspecified role bait bait N/A 722812 unspecified role unspecified role prey prey physical association physical association false false false 2 B krt5-alox12-1 Exogenous 12-LOX coimmunoprecipitates with KRT5 in A431 lysates 722807 N/A 722811 unspecified role unspecified role prey prey N/A 722812 unspecified role unspecified role bait bait physical association physical association false false false 2 B P18054 tansfection P18054 over-expression alox12-lmna-2 12-LOX coimmunoprecipitates LMNA in A431 lysates 722807 N/A 722813 unspecified role unspecified role prey prey N/A 722812 unspecified role unspecified role bait bait physical association physical association false false false 4 This interaction was observed in nuclear extracts and not cytoplasmic extracts. lmna-alox12 12-LOX coimmunoprecipitates with LMNA in nuclear A431 extracts 722807 N/A 722812 unspecified role unspecified role prey prey N/A 722813 unspecified role unspecified role bait bait physical association physical association false false false 4 This interaction was observed in nuclear extracts and not cytoplasmic extracts. alox12-krt5 12-LOX interacts with KRT5 in a yeast two-hybrid assay 722808 N/A 722811 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 722812 unspecified role unspecified role bait bait physical association physical association false false false alox12-lmna 12-LOX interacts with Lamin A in a yeast two-hybrid assay 722808 N/A 722812 unspecified role unspecified role bait bait N/A 722813 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false alox12-itgb4 12-LOX interacts with ITGB4 in a yeast two-hybrid assay 722808 N/A 722812 unspecified role unspecified role bait bait N/A 722814 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The range of the cytoplasmic domain of integrin beta-4 has been taken from the UniProtKB entry for the protein. alox12-trib1 12-LOX interacts with C8FW in a yeast two-hybrid assay 722808 N/A 722815 unspecified role unspecified role prey prey N/A 722812 unspecified role unspecified role bait bait physical association physical association false false false alox12-itgb4-2 12-LOX interacts with ITGB4 in a directed yeast two-hybrid assay 722809 N/A 722812 unspecified role unspecified role bait bait N/A 722814 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The range of the cytoplasmic domain of integrin beta-4 has been taken from the UniProtKB entry for the protein. Fig. 1, Table 1 alox12-krt5-1 12-LOX interacts with KRT5 in a directed yeast two-hybrid assay 722809 N/A 722812 unspecified role unspecified role bait bait N/A 722811 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1, Table 1 alox12-trib1-1 12-LOX interacts with C8FW in a directed yeast two-hybrid assay 722809 N/A 722815 unspecified role unspecified role prey prey N/A 722812 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1, Table 1 alox12-lmna-1 12-LOX interacts with Lamin A in a directed yeast two-hybrid assay 722809 N/A 722813 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 722812 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1, Table 1 alox12-krt5-4 12-LOX and KRT5 colocalize in A431 cells 722810 N/A 722812 unspecified role unspecified role neutral component neutral component N/A 722811 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3