IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tang-2006-8 Critical role for Daxx in regulating Mdm2. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-1 Critical role for Daxx in regulating Mdm2. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-2 Critical role for Daxx in regulating Mdm2. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-4 Critical role for Daxx in regulating Mdm2. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis Lysate from p53-negative, Mdm2-negative MEFs were used for coimmunoprecipitation. xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-6 Critical role for Daxx in regulating Mdm2. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis HA�Hausp and GST�Mdm2 were cotransfected into H1299 in the presence or absence of Flag�Daxx. Cells were treated with 20 uM MG-132 for 4 h and the lysates were immunoprecipitated with M2 beads (lanes 3 and 4). Flag�Daxx and its binding complex were eluted using Flag peptide and the eluant was subject to a secondary immunoprecipitation with either anti-Mdm2 or a control antibody. Lysate from p53-negative, Mdm2-negative MEFs were used for coimmunoprecipitation. xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-3 Critical role for Daxx in regulating Mdm2. in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis HeLa S3 lysates were incubated with M2 beads (control) or M2 beads coated with recombinant Flag�Daxx. The bound proteins were resolved on SDS�PAGE and visualized by Coomassie blue staining. The 135 K protein band in the Flag�Daxx lane was identified as Hausp by mass spectrometry xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-5 Critical role for Daxx in regulating Mdm2. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunostaining immunostaining Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-7 Critical role for Daxx in regulating Mdm2. mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis Wild-type and Daxx-deficient ES cells were treated with 5 uM MG-132 for 6 h. Cell lysates were immunoprecipitated with an anti- Hausp antibody and the control antibody. xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-9 Critical role for Daxx in regulating Mdm2. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence fluorescence technology fluorescence immunostaining immunostaining Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis U2OS cells were treated with ALLN for 4 h or left untreated. (ALLN stabilises Mdm2). Cells were stained with a rabbit anti-Daxx and a mouse anti-Mdm2 antibodies. xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-10 Critical role for Daxx in regulating Mdm2. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology tang-2006-11 Critical role for Daxx in regulating Mdm2. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tang J., Qu LK., Zhang J., Wang W., Michaelson JS., Degenhardt YY., El-Deiry WS., Yang X. Apoptosis - Interactions involving proteins with a function related to apoptosis Lysate from p53-negative, Mdm2-negative MEFs were used for coimmunoprecipitation. xyang@mail.med.upenn.edu Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation BioCreative - Critical Assessment of Information Extraction systems in Biology p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP ubp7_human Ubiquitin carboxyl-terminal hydrolase 7 USP7 HAUSP Ubiquitin thioesterase 7 Ubiquitin-specific-processing protease 7 Deubiquitinating enzyme 7 Herpesvirus-associated ubiquitin-specific protease protein protein human Homo sapiens Human MNHQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRITQNPVINGNVALSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKKAVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDIQEKEFEKFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKRSRYTYLEKAIKIHN daxx_human Death domain-associated protein 6 DAXX BING2 DAP6 Daxx Fas death domain-associated protein ETS1-associated protein 1 protein protein human Homo sapiens Human MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPSGMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCKKSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCIPSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD ubp7-daxx-7 506079 N/A 506090 unspecified role unspecified role prey prey N/A 506091 unspecified role unspecified role prey prey N/A 506092 unspecified role unspecified role bait bait N/A 506093 unspecified role unspecified role prey prey association association false false false 4 C, 5 A (left panel), 5 B left panel The interaction of endogenous Mdm2 and Hausp requires Daxx. These results, in combination with the Mdm2- independent Daxx�Hausp interaction (Fig. 3b), suggested that Daxx is the central component of the Mdm2�Daxx�Hausp complex and physically connects Hausp to Mdm2 in vivo. DNA damage caused by etoposide dissociates Daxx and Hausp from Mdm2. When the expression of ATM was decreased by siRNA, the interactions of Daxx with both Mdm2 and Hausp were less affected by etoposide. ubp7-daxx-8 506079 N/A 506091 unspecified role unspecified role bait bait N/A 506090 unspecified role unspecified role prey prey N/A 506093 unspecified role unspecified role prey prey association association false false false S2 A and S3 A Hausp does not affect this interaction as show when U2OS cells were transfected with a Hausp-specific siRNA or a control siRNA. ubp7-daxx-9 506079 N/A 506092 unspecified role unspecified role bait bait N/A 506090 unspecified role unspecified role prey prey N/A 506091 unspecified role unspecified role prey prey N/A 506093 unspecified role unspecified role prey prey association association false false false S2 E and S4 C Etoposide stops Mdm2 from associating with the complex. Wortmannin prevents this disrupting effect. mdm2-daxx-2 506079 N/A 506091 unspecified role unspecified role prey prey N/A 506090 unspecified role unspecified role prey prey N/A 506093 unspecified role unspecified role bait bait association association false false false S4 B Etoposide disrupts the Daxx-Mdm2 interaction and the Daxx-p53 interaction. mdm2-daxx 506080 N/A 506093 unspecified role unspecified role prey prey N/A 506090 unspecified role unspecified role prey prey N/A 506091 unspecified role unspecified role bait bait association association false false false 2 A and S5 E The interaction between Mdm2 and Daxx without p53 was shown in p53-negative HCT116 cells. Thus the interaction takes place regardless of p53 status. ubp7-daxx 506080 N/A 506090 unspecified role unspecified role prey prey N/A 506093 unspecified role unspecified role bait bait N/A 506092 unspecified role unspecified role prey prey N/A 506091 unspecified role unspecified role prey prey association association false false false 3 B and S5g The interaction between all the 4 above proteins occurred in the p53 wild-type HCT116 cells and the interaction without p53 occurred in the HCY116 p53-negative cells. mdm2-daxx-1 506081 N/A 506091 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 506093 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2 B ubp7-daxx-3 506081 N/A 506093 unspecified role unspecified role prey prey N/A 506092 unspecified role unspecified role bait bait direct interaction direct interaction false false false 3 D ubp7-daxx-1 506082 N/A 506093 unspecified role unspecified role bait bait N/A 506092 unspecified role unspecified role prey prey physical association physical association false false false 3 B and S5g ubp7-daxx-5 506082 N/A 506091 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 506092 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 506093 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4 A Overexpression of Daxx enhances the interaction between Mdm2 and Daxx. ubp7-daxx-4 506083 N/A 506091 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 506092 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 506093 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3 E and S5h daxx-mdm2-1 506083 N/A 506091 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 506093 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region contains second paired amphipathic helix domain (PAH2) required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false S2 D Deletion analyses revealed that the main binding domain for Mdm2 resides in a small region of Daxx (amino acids 157�260) that encompasses the second paired amphipathic helix domain (PAH2). daxx-usp7 506084 N/A 506093 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 506092 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false 3 A ubp7-daxx-2 506085 N/A 506092 unspecified role unspecified role neutral component neutral component c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 506093 unspecified role unspecified role neutral component neutral component n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 3 C ubp7-daxx-6 506086 N/A 506090 unspecified role unspecified role prey prey N/A 506091 unspecified role unspecified role prey prey N/A 506092 unspecified role unspecified role bait bait N/A 506093 unspecified role unspecified role prey prey association association false false false 4 B and S5i The Hausp-Mdm2 interaction was increased in the presence of Daxx. These results, in combination with the Mdm2- independent Daxx�Hausp interaction (Fig. 3b), suggested that Daxx is the central component of the Mdm2�Daxx�Hausp complex and physically connects Hausp to Mdm2 in vivo. daxx-mdm2 506087 N/A 506091 unspecified role unspecified role neutral component neutral component N/A 506093 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Endogenous Daxx and Mdm2 were mostly found in the nucleus. S2B ubp7-daxx-10 506088 N/A 506093 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal contain the first paired amphipathic helix domain (PAH1) sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false c-terminal acid-rich region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 506092 unspecified role unspecified role prey prey physical association physical association false false false S2 F Two regions of Daxx associate with Hausp: the N-terminal 160 residues, containing the first paired amphipathis helix domain (PAH1) and residues 347-760, containing the acid-rich region. usp7-hausp 506089 N/A 506093 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 506091 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 506092 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false S4 E Mutations of Mdm2 at Ser395 do not affect the interactions of Mdm2 with Daxx and Hausp.