IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ mcguire-2006-5 Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. mcguire-2006-1 Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. mcguire-2006-2 Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. mcguire-2006-3 Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. in vitro In vitro pull down pull down predetermined predetermined participant predetermined McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. mcguire-2006-4 Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology Hypothalamic tissue was used for HAP1 immunoprecipitation. xiaoli@genetics.emory.edu rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. mcguire-2006-6 Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. p54256-2 Huntingtin-associated protein 1 Short Hap1 protein protein rat Rattus norvegicus (Rat) Rat MRPKDQVQSSAGDGTGSGDPATGTPTTQPAADPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGGSFCRSRIRGDSDAPWTRYIFQGPYGPRATGLGTGRAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPLPRKKITEDDIKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDEEDEEDEEEEEGEEEEREGQRDQDQQHDHPYGAPKPPPKAETLHHCPQLEALKQKLKLLEEENDHLREEASHLDNLEDKEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQQLASEKGVHPESLRAGSHMQDYGSRPRERQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERCDTRCREERKKEQGTMPPPPVQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGPAEETEQASEETEAWEEVEPEVDEATRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKGECSRRGHPPASGTSYRSSTL Contains a phosphothreonine at position 598. kif5c_rat Kinesin heavy chain isoform 5C Kinesin heavy chain neuron-specific 2 Kif5c Nkhc2 protein protein rat Rattus norvegicus (Rat) Rat MADPAECSIKVMCRFRPLNEAEILRGDKFIPKFKGEETVVIGQGKPYVFDRVLPPNTTQEQVYNACAKQIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIAHDIFDHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLAVHEDKNRVPYVKGCTERFVSSPEEVMDVIDEGKANRHVAVTNMNEHSSRSHSIFLINIKQENVETEKKLSGKLYLVDLAGSEKV o35668-2 Huntingtin-associated protein 1 Short Hap1 protein protein mouse Mus musculus Mouse MRPKEQVQSGAGDGTGSGDPAAGTPTTQPAVGPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGSFCRSMIIGDSDAPWTRYVFQGPYGPRATGLGTGKAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPPPTKKITEDDVKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDDDEEDEEDEEEGEEEEREGQRDQDQQHDHPYGAPKPHPKAETAHRCPQLETLQQKLRLLEEENDHLREEASHLDNLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQMLASEKGIHSESLRAGSYMQDYGSRPRDRQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERRDTHCREGRKKEQRAMPPPPAQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGQAEENEQASEETEAWEEVEPEVDETTRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKGECSRRGHPPASGTSFRSSTI Contains a phosphothreonine at position 598. b2gv74_rat Kinesin light chain Klc2 Klc2_predicted rCG_48462 protein protein rat Rattus norvegicus (Rat) Rat MATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLASHEAGEAEPGSQERCLLLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGENKPIWMHAEEREESKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRSRKQGLDPASQTKVVELLKDGSGGRGDRRGSRDVAGGAGPRSESDLEESGPAAEWSGDGSGSLRRSGSFGKLRDALRRSSEMLVRKLQGGGPQEPPNSRMKRASSLNFLNKSVEEPVQPGGTGLSDSRTLSSSSMDLSRRSSLVG klc1_mouse Kinesin light chain 1 Klc1 Kns2 protein protein mouse Mus musculus Mouse MYDNMSTMVYIKEEKLENVTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSSMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQNVRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDSDSSKEPLDDLFPNDEDEPGQGIQHSDSSAAAARQGYEIPARLRTLHNLVIQYASQGRYEVAVPSCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALGIYQTKLGPDRTPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEAEFGSVDDENKPIWMHAEEREECKGKQKDGSAFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPESMEKRRSRESLNMDVVKYESGPDGGEEA hap1_mouse Huntingtin-associated protein 1 Hap1 protein protein mouse Mus musculus Mouse MRPKEQVQSGAGDGTGSGDPAAGTPTTQPAVGPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGSFCRSMIIGDSDAPWTRYVFQGPYGPRATGLGTGKAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPPPTKKITEDDVKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDDDEEDEEDEEEGEEEEREGQRDQDQQHDHPYGAPKPHPKAETAHRCPQLETLQQKLRLLEEENDHLREEASHLDNLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQMLASEKGIHSESLRAGSYMQDYGSRPRDRQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERRDTHCREGRKKEQRAMPPPPAQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGQAEENEQASEETEAWEEVEPEVDETTRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKDSPTPQQQTNMGGGILEQQPRVPTQDSQRLEEDRATHSPSAREEEGPSGAT klc2_mouse Kinesin light chain 2 Klc2 protein protein mouse Mus musculus Mouse MATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLASHEAGEAEPGSQERCLLLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEMLPQEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGENKPIWMHAEEREESKDKRRDRRPMEYGSWYKACKVDSPTVNTTLRTLGALYRPEGKLEAAHTLEDCASRSRKQGLDPASQTKVVELLKDGSGRGHRRGSRDVAGPQSESDLEESGPAAEWSGDGSGSLRRSGSFGKLRDALRRSSEMLVRKLQGGGPQEPNSRMKRASSLNFLNKSVEEPVQPGGRVFLTAAL p54256-1 Huntingtin-associated protein 1 Long Hap1 protein protein rat Rattus norvegicus (Rat) Rat MRPKDQVQSSAGDGTGSGDPATGTPTTQPAADPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGGSFCRSRIRGDSDAPWTRYIFQGPYGPRATGLGTGRAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPLPRKKITEDDIKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDEEDEEDEEEEEGEEEEREGQRDQDQQHDHPYGAPKPPPKAETLHHCPQLEALKQKLKLLEEENDHLREEASHLDNLEDKEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQQLASEKGVHPESLRAGSHMQDYGSRPRERQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERCDTRCREERKKEQGTMPPPPVQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGPAEETEQASEETEAWEEVEPEVDEATRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKDSPAPQQQTNMGGGIVEQQPIVPTQDSQRLEEDRATHSPSAREEEGPSGAT hap1-khc 501628 N/A 501634 unspecified role unspecified role neutral component neutral component N/A 501635 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false The colocalisation was observed in the presence of NGF treatment. 2B hap1-khc-1 501628 N/A 501635 unspecified role unspecified role neutral component neutral component N/A 501636 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false The colocalisation was observed in the presence of NGF treatment. 3 hap1-klc2 501629 N/A 501637 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 501636 unspecified role unspecified role bait bait physical association physical association false false false 1 hap1-klc2-1 501630 N/A 501636 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 501637 unspecified role unspecified role prey prey physical association physical association false false false 1B hap1-klc2-2 501631 N/A 501637 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 501636 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1C hap1-klc2-3 501632 N/A 501638 unspecified role unspecified role prey prey N/A 501639 unspecified role unspecified role bait bait N/A 501640 unspecified role unspecified role prey prey association association false false false 1D The antibody used reacts with both KLC-1 and KLC-2 in the brain. KLC-1 is the major form of KLC in the hypothalamus and was coprecipitated by the antibody to HAP1. klc2-hap1 501633 N/A 501637 unspecified role unspecified role prey prey N/A 501634 unspecified role unspecified role bait bait N/A 501641 unspecified role unspecified role prey prey association association false false false 2C