IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gengenbache-2006-1 Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights. ecobb Escherichia coli (strain B / BL21) pull down pull down western blot western blot Immuno blot Immuno blot Gengenbacher M., Fitzpatrick TB., Raschle T., Flicker K., Sinning I., Muller S., Macheroux P., Tews I., Kappes B. BioCreative - Critical Assessment of Information Extraction systems in Biology ivo.tews@bzh.uni-heidelberg.de J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation pdx2_plaf7 Pyridoxal 5'-phosphate synthase subunit Pdx2 pdx2 Pyridoxal 5'-phosphate synthase glutaminase subunit PF11_0169 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MSEITIGVLSLQGDFEPHINHFIKLQIPSLNIIQVRNVHDLGLCDGLVIPGGESTTVRRCCAYENDTLYNALVHFIHVLKKPIWGTCAGCILLSKNVENIKLYSNFGNKFSFGGLDITICRNFYGSQNDSFICSLNIISDSSAFKKDLTAACIRAPYIREILSDEVKVLATFSHESYGPNIIAAVEQNNCLGTVFHPELLPHTAFQQYFYEKVKNYKYS pdx1_plaf7 Pyridoxal 5'-phosphate synthase subunit Pdx1 pdx1 PFF1025c protein protein plaf7 Plasmodium falciparum (isolate 3D7) MENHKDDAVLLKHGWCEMLKGGVIMDVKSVEQAKIAEEAGAIGVMVLENIPSELRNKEGVARSVDPSKVEEIKKCVSINVLAKVRIGHFVEAQILEELKIDMIDESEVLTIADEMHHIDKHKFKTPFVCGCTNLGEALRRISEGASMIRTKGEAGTGNIIEAIKHIRTVNNEIKYLCSLSDSEVYHFAKKINAPIDLVLLTKKLKRLPVVNFAAGGVATPADAAMCMQLGMDGVFVGSGIFESENPRKMAASIVSAVSNFNNPKILLDVSMNLGKAMCGSTRVSDKWKNKNEEHTKFLTPQ pdx1-pdx2-1 Interaction between PDX1 and PDX2 demonstrated by pulldown 500253 N/A 500254 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 500255 unspecified role unspecified role prey prey Stoichiometry: 1.0 physical association physical association false false false Fig. 3B