IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ han-2006-1 Shank2 associates with and regulates Na+/H+ exchanger 3. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Han W., Kim KH., Jo MJ., Lee JH., Yang J., Doctor RB., Moe OW., Lee J., Kim E., Lee MG. BioCreative - Critical Assessment of Information Extraction systems in Biology The intensity of the binding was determined by the induction of the yeast reporter genes HIS3 and beta-galactosidase. mlee@yumc.yonsei.ac.kr J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation han-2006-2 Shank2 associates with and regulates Na+/H+ exchanger 3. crigr-ps120 Cricetulus griseus ps120 fibroblasts ps120 PS120 hamster fibroblasts anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Han W., Kim KH., Jo MJ., Lee JH., Yang J., Doctor RB., Moe OW., Lee J., Kim E., Lee MG. BioCreative - Critical Assessment of Information Extraction systems in Biology The intensity of the binding was determined by the induction of the yeast reporter genes HIS3 and beta-galactosidase. mlee@yumc.yonsei.ac.kr J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation han-2006-3 Shank2 associates with and regulates Na+/H+ exchanger 3. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Han W., Kim KH., Jo MJ., Lee JH., Yang J., Doctor RB., Moe OW., Lee J., Kim E., Lee MG. BioCreative - Critical Assessment of Information Extraction systems in Biology The intensity of the binding was determined by the induction of the yeast reporter genes HIS3 and beta-galactosidase. mlee@yumc.yonsei.ac.kr J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation shan2_rat SH3 and multiple ankyrin repeat domains protein 2 Cortactin-binding protein 1 Proline-rich synapse-associated protein 1 GKAP/SAPAP-interacting protein SPANK-3 Shank2 Cortbp1 protein protein rat Rattus norvegicus (Rat) Rat MKSLLNAFTKKEVPFREAPAYSNRRRRPPNTLAAPRVLLRSNSDNNLNAGAPEWAVCSAATSHRSLSPQLLQQTPSKPDGATKSLGSYAPGPRSRSPSLNRLGGAGEDGKRPQPPHWHVGSPFTPGANKDSLSTFEYPGPRRKLYSAVPGRLFVAIKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDAASDCIIEDKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTAELEELGLSLVDKASVRKKKDKPEEIVPASKPSRTAENVAIESRVATIKQRPTSRCFPAASDVNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPRSPTPRVYGTIKPAFNQNPVAKVPPATRSDTVATMMREKGMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQATEPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRMQPSKFPEEGGFGDEDETEQPLLPTPGAAPRELENHFLGGGEAGAQGEAGGPLSSTSKAKGPESGPAAALKSSSPASPENYVHPLTGRLLDPSSPLALALSARDRAMQESQQGHKGEAPKADLNKPLYIDTKMRPSVESGFPPVTRQNTRGPLRRQETENKYETDLSKDRRADDKKNMLINIVDTAQQKSAGLLMVHTVDIPVAGPPLEEEEDREDGDTKPDHSPSTVPEGVPKTEGALQISAAPEPAAAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTEPLPPPLEFANSFDIPDDRAASVPALADLVKQKKSDTPQPPSLNSSQPANSTDSKKPAGISNCLPSSFLPPPESFDAVTDSGIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGESMDTCTVYADGQAFVVDKPPVPPKPKMKPIVHKSNALYQDTLPEEDTDGFVIPPPAPPPPPGSAQAGVAKVIQPRTSKLWGDVTEVKSPILSGPKANVISELNSILQQMNRGKSVKPGEGLELPVGAKSANLAPRSPEVMSTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGPRRAPSPVVSPTELSKEILPTPPSAAAASPSPTLSDVFSLPSQSPAGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKETFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR sl9a3_rat Sodium/hydrogen exchanger 3 Slc9a3 Nhe3 Na(+)/H(+) exchanger 3 Solute carrier family 9 member 3 protein protein rat Rattus norvegicus (Rat) Rat MWHPALGPGWKPLLALAVAVTSLRGVRGIEEEPNSGGSFQIVTFKWHHVQDPYIIALWILVASLAKIVFHLSHKVTSVVPESALLIVLGLVLGGIVWAADHIASFTLTPTLFFFYLLPPIVLDAGYFMPNRLFFGNLGTILLYAVIGTIWNAATTGLSLYGVFLSGLMGELKIGLLDFLLFGSLIAAVDPVAVLAVFEEVHVNEVLFIIVFGESLLNDAVTVVLYNVFESFVTLGGDAVTGVDCVKGIVSFFVVSLGGTLVGVIFAFLLSLVTRFTKHVRIIEPGFVFVISYLSYLTSEMLSLSAILAITFCGICCQKYVKANISEQSATTVRYTMKMLASGAETIIFMFLGISAVDPVIWTWNTAFVLLTLVFISVYRAIGVVLQTWILNRYRMVQLETIDQVVMSYGGLRGAVAYALVVLLDEKKVKEKNLFVSTTLIVVFFTVIFQGLTIKPLVQWLKVKRSEQREPKLNEKLHGRAFDHILSAIEDISGQIGHNYLRDKWSNFDRKFLSKVLMRRSAQKSRDRILNVFHELNLKDAISYVAEGERRGSLAFIRSPSTDNMVNVDFSTPRPSTVEASVSYFLRENVSAVCLDMQSLEQRRRSIRDTEDMVTHHTLQQYLYKPRQEYKHLYSRHELTPNEDEKQDKEIFHRTMRKRLESFKSAKLGINQNKKAAKLYKRERAQKRRNSSIPNGKLPMENLAHNFTIKEKDLELSEPEEATNYEEISGGIEFLASVTKDVASDSGAGIDNPVFSPDEDLDPSILSRVPPWLSPGETVVPSQRARVQIPNSPSNFRRLTPFRLSNKSVDSFLQADGPEEQLQPASPESTHM slc9a3 Chinese hamster Slc9a3 (NHE3) protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster An amino acid sequence which is not represented in UniProt slc9a3-shank2-1 Interaction between Slc9a3 (NHE3) and Shank2 demonstrated by yeast 2 hybrid 493111 N/A 493114 unspecified role unspecified role prey prey pdz region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 493115 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 493121 493119 physical association physical association false false false S1 slc9a3-shank2-2 Interaction between hamster Slc9a3 (NHE3) and rat Shank2 demonstrated by yeast 2 hybrid 493112 N/A 493116 unspecified role unspecified role prey prey N/A 493114 unspecified role unspecified role bait bait physical association physical association false false false 1A slc9a3-shank2-3 Interaction between Slc9a3 (NHE3) and Shank2 demonstrated by yeast 2 hybrid 493113 N/A 493114 unspecified role unspecified role prey prey pdz region sufficient to bind sufficient binding region sufficient to bind range range range range false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 493115 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false 1B Association rate constant=3.2x10EXP-4M-1s-1 Dissociation rate constant=3.7x10EXP-3s-1