IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ koike_takes-2006-2 Leu309 plays a critical role in the encapsulation of substrate protein into the internal cavity of GroEL. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Koike-Takeshita A., Shimamura T., Yokoyama K., Yoshida M., Taguchi H. BioCreative - Critical Assessment of Information Extraction systems in Biology A number of residues in both proteins were replaced by cysteine, proteins then cross-linked when dithiothreotol was removed from the reaction mixture. myoshida@res.titech.ac.jp J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation koike_takes-2006-3 Leu309 plays a critical role in the encapsulation of substrate protein into the internal cavity of GroEL. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Koike-Takeshita A., Shimamura T., Yokoyama K., Yoshida M., Taguchi H. BioCreative - Critical Assessment of Information Extraction systems in Biology A number of residues in both proteins were replaced by cysteine, proteins then cross-linked when dithiothreotol was removed from the reaction mixture. myoshida@res.titech.ac.jp J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation koike_takes-2006-1 Leu309 plays a critical role in the encapsulation of substrate protein into the internal cavity of GroEL. in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Koike-Takeshita A., Shimamura T., Yokoyama K., Yoshida M., Taguchi H. BioCreative - Critical Assessment of Information Extraction systems in Biology A number of residues in both proteins were replaced by cysteine, proteins then cross-linked when dithiothreotol was removed from the reaction mixture. myoshida@res.titech.ac.jp J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation mdh_ecoli Malate dehydrogenase mdh b3236 JW3205 protein protein ecoli Escherichia coli (strain K12) MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAVDLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLVQQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAELKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVEERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK ch60_ecoli Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 GroEL protein groL mopA JW4103 b4143 protein protein ecoli Escherichia coli (strain K12) MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM CD3A0FB505F74AD1 ch10_ecoli Co-chaperonin GroES groES 10 kDa chaperonin Chaperonin-10 groS mopB JW4102 b4142 protein protein ecoli Escherichia coli (strain K12) MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA 76829E09B11217EF glpe_ecoli Thiosulfate sulfurtransferase GlpE glpE b3425 JW3388 protein protein ecoli Escherichia coli (strain K12) MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLTNDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPAEVAYGA grol-mdh-1 Interaction between GroEL and MDH demonstrated by protein cross-linking 500507 N/A 500510 unspecified role unspecified role neutral component neutral component region cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 500511 unspecified role unspecified role neutral component neutral component P0A6F5:p.Leu309Lys mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 5 grol-gros-2 Interaction between GroEL and GroES demonstrated by gel filtration 500507 N/A 500511 unspecified role unspecified role neutral component neutral component P0A6F5:p.Leu309Lys mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 500512 unspecified role unspecified role neutral component neutral component region cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 5C grol-glpe-1 Interaction between GroEL and GLPE demonstrated by ultracentrifugation 500508 N/A 500511 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 500513 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Interaction requires presence of ATP 6 grol-glpe-2 Interaction between GroEL and GLPE demonstrated by ultracentrifugation 500508 N/A 500512 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 500511 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 500513 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Interaction stabilised by the presence of beryllium fluoride, a phosphate analogue 6 grol-gros-1 Interaction between GroEL and GroES demonstrated by protein cross-linking 500509 N/A 500512 unspecified role unspecified role neutral component neutral component P0A6F9:p.Thr28Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0A6F9:p.Gly29Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P0A6F9:p.Ser30Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 500511 unspecified role unspecified role neutral component neutral component P0A6F5:p.Met307Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0A6F5:p.Glu308Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0A6F5:p.Leu309Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0A6F5:p.Glu310Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P0A6F5:p.Glu308Cys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 500541 500535 direct interaction direct interaction false false false Interaction requires presence of ATP 2