IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ panizzi-2006-1 Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Panizzi P., Friedrich R., Fuentes-Prior P., Kroh HK., Briggs J., Tans G., Bode W., Bock PE. BioCreative - Critical Assessment of Information Extraction systems in Biology Fluorescence titrations of an array of ProT analogs with was carried out. paul.bock@vanderbilt.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation panizzi-2006-2 Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. in vitro In vitro competition binding competition binding predetermined predetermined participant predetermined Panizzi P., Friedrich R., Fuentes-Prior P., Kroh HK., Briggs J., Tans G., Bode W., Bock PE. BioCreative - Critical Assessment of Information Extraction systems in Biology Fluorescence titrations of an array of ProT analogs with was carried out. paul.bock@vanderbilt.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation panizzi-2006-3 Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Panizzi P., Friedrich R., Fuentes-Prior P., Kroh HK., Briggs J., Tans G., Bode W., Bock PE. BioCreative - Critical Assessment of Information Extraction systems in Biology Fluorescence titrations of an array of ProT analogs with was carried out. paul.bock@vanderbilt.edu J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation thrb_human Prothrombin F2 Coagulation factor II protein protein human Homo sapiens Human MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE q846v4_staau protein protein staau Staphylococcus aureus IVTKDYSKESRVNENSKYGTLISDWYLKGRLTSLESQFINALGILETYHYGEKEYKDAKDKLMTRILGEDQYLLERKKVQYEEYKKLYKKYKEENPTSKVKMKTFDQYTIEDLTMREYNELTESLKSAVKDFEKDVEIIENQHHDLKPFTDEMEEKATARVDDLANKAYSVYFAFVRDTQHKTEALELKAKVDLVLGDEDKPHRISNERIEKEMIKDLESIIEDFFIETGLNKPDNITSYDSSKHHYKNHSEGFEALVKETREAVTNANDSWKTKTVKKYGETVTKPPVVKEEKKVEEPQAPKVSEKVDVQETVGTEETTQPVAQPLVKIPQGTIYGETVKGPEYLTMENKTLQGEIVQGPDFPTMEQNRPSLSDNYTQPTTPNPILKGLEGSSSKLEIKPQGTESTLKGTQGESSDIEVKPQASETTEASHYPARPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTYKKPSKTNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTYKKPSETNAYNVTTHADGTATYGSRVTK sc-f2-3 505028 N/A 505031 unspecified role unspecified role neutral component neutral component human-plasma homo sapiens blood plasma plasma blood plasma N/A 505032 unspecified role unspecified role neutral component neutral component n terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false 2, 3 Analogues of ProT were competed out by SC. sc-f2-4 505029 N/A 505031 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma N/A 505032 unspecified role unspecified role prey prey n terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 6 Analogues of ProT were competed out by SC. sc-f2-5 505030 N/A 505031 self self unspecified role unspecified role human-plasma homo sapiens blood plasma plasma blood plasma N/A 505032 unspecified role unspecified role ancillary ancillary n terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false cleavage cleavage reaction cleavage false false false 4, 5 Increasing concentration of SC resulted in increased self cleavage of ProThrombin.