IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chen-1996-1 A bacterial invasin induces macrophage apoptosis by binding directly to ICE. in vitro In vitro pull down pull down monoclonal western monoclonal antibody western blot monoclonal western Chen Y., Smith MR., Thirumalai K., Zychlinsky A. Apoptosis - Interactions involving proteins with a function related to apoptosis EMBO J. (0261-4189) 1996 chen-1996-2 A bacterial invasin induces macrophage apoptosis by binding directly to ICE. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Chen Y., Smith MR., Thirumalai K., Zychlinsky A. Apoptosis - Interactions involving proteins with a function related to apoptosis EMBO J. (0261-4189) 1996 chen-1996-3 A bacterial invasin induces macrophage apoptosis by binding directly to ICE. mouse-j774a-1 Mus musculus macrophage j774a-1 mouse-macrophage coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip monoclonal western monoclonal antibody western blot monoclonal western Chen Y., Smith MR., Thirumalai K., Zychlinsky A. Apoptosis - Interactions involving proteins with a function related to apoptosis This experiment is carried out with Shigella flexneri strain M90T serotype 5. Negative controls are performed with the non-invasive derivate of M90T strain cured of the virulence plasmid carrying the IPA-B/C/D genes. EMBO J. (0261-4189) 1996 casp1_mouse Caspase-1 Interleukin-1 beta convertase Interleukin-1 beta-converting enzyme p45 Casp1 Il1bc protein protein mouse Mus musculus Mouse MADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKIKLANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAGILELQSAPSAETFVATEDSKGGHPSSSETKEEQNKEDGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTRTRLALIICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFAACPEHKTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPKVIIIQACRGEKQGVVLLKDSVRDSEEDFLTDAIFEDDGIKKAHIEKDFIAFCSSTPDNVSWRHPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLTKRFYLFPGH ipab_shifl Invasin IpaB 62 kDa antigen ipaB CP0128 protein protein shifl Shigella flexneri MHNVSTTTTGFPLAKILTSTELGDNTIQAANDAANKLFSLTIADLTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKITAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQIQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLEKEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQSLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASLALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVDSKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFLKNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGSVASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQANRTDVAKAILQQTTA ipab-casp1-1 pull down of shigella ipa-b and mouse caspase 1 169853 N/A 169856 unspecified role unspecified role prey prey 35s radiolabel tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse-j774a-1 Mus musculus macrophage j774a-1 mouse-macrophage N/A 169857 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false ipab-casp1-2 filter binding of shigella ipa-b and mouse caspase 1 169854 N/A 169856 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 169857 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 32p label of gst tag radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false The species of casp1 clone is not mentioned however It is assumed to be mouse casp1 since other experiments refers to mouse casp1. ipab-casp1-3 co-immunprecipitation of shigella ipa-b and mouse caspase 1 after infection of J774a cells 169855 N/A 169856 unspecified role unspecified role prey prey mouse-j774a-1 Mus musculus macrophage j774a-1 mouse-macrophage N/A 169857 unspecified role unspecified role bait bait shifl Shigella flexneri physical association physical association false false false Casp1 is immunoprecipitated only after infection of wild type shigella, and in not detected when the avirulent derivative missing the Ipa B/C/D genes is used. Casp1 induces the release of interleukin-1 beta (apoptosis signal) following wild type shigella infection. Microinjected IpaB is sufficient to induce apoptosis of J774-a cells. Thus this interaction is believed to induce apoptosis.