IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rothe-1994-1 A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rothe M., Wong SC., Henzel WJ., Goeddel DV. Apoptosis - Interactions involving proteins with a function related to apoptosis Directed yeast-2-hybrid Cell (0092-8674) 1994 rothe-1994-2 A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rothe M., Wong SC., Henzel WJ., Goeddel DV. Apoptosis - Interactions involving proteins with a function related to apoptosis Directed yeast-2-hybrid Cell (0092-8674) 1994 rothe-1994-3 A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor. in vitro In vitro pull down pull down edman degradation edman degradation Rothe M., Wong SC., Henzel WJ., Goeddel DV. Apoptosis - Interactions involving proteins with a function related to apoptosis Directed yeast-2-hybrid Cell (0092-8674) 1994 tnr1b_human Tumor necrosis factor receptor superfamily member 1B TNFRSF1B TNFBR TNFR2 Tumor necrosis factor receptor 2 Tumor necrosis factor receptor type II p75 p80 TNF-alpha receptor protein protein human Homo sapiens Human MAPVAVWAALAVGLELWAAAHALPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGDFALPVGLIVGVTALGLLIIGVVNCVIMTQVKKKPLCLQREAKVPHLPADKARGTQGPEQQHLLITAPSSSSSSLESSASALDRRAPTRNQPQAPGVEASGAGEARASTGSSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTMGDTDSSPSESPKDEQVPFSKEECAFRSQLETPETLLGSTEEKPLPLGVPDAGMKPS traf1_human TNF receptor-associated factor 1 TRAF1 EBI6 Epstein-Barr virus-induced protein 6 protein protein human Homo sapiens Human MASSSGSSPRPAPDENEFPFGCPPTVCQDPKEPRALCCAGCLSENPRNGEDQICPKCRGEDLQSISPGSRLRTQEKAHPEVAEAGIGCPFAGVGCSFKGSPQSVQEHEVTSQTSHLNLLLGFMKQWKARLGCGLESGPMALEQNLSDLQLQAAVEVAGDLEVDCYRAPCSESQEELALQHFMKEKLLAELEGKLRVFENIVAVLNKEVEASHLALATSIHQSQLDRERILSLEQRVVELQQTLAQKDQALGKLEQSLRLMEEASFDGTFLWKITNVTRRCHESACGRTVSLFSPAFYTAKYGYKLCLRLYLNGDGTGKRTHLSLFIVIMRGEYDALLPWPFRNKVTFMLLDQNNREHAIDAFRPDLSSASFQRPQSETNVASGCPLFFPLSKLQSPKHAYVKDDTMFLKCIVETST traf2_human TNF receptor-associated factor 2 TRAF2 TRAP3 Tumor necrosis factor type 2 receptor-associated protein 3 E3 ubiquitin-protein ligase TRAF2 RING-type E3 ubiquitin transferase TRAF2 protein protein human Homo sapiens Human MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTYDGVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL tnr1b_mouse Tumor necrosis factor receptor superfamily member 1B Tumor necrosis factor receptor 2 Tumor necrosis factor receptor type II p75 p80 TNF-alpha receptor Tnfrsf1b Tnfr2 Tnfr-2 protein protein mouse Mus musculus Mouse MAPAALWVALVFELQLWATGHTVPAQVVLTPYKPEPGYECQISQEYYDRKAQMCCAKCPPGQYVKHFCNKTSDTVCADCEASMYTQVWNQFRTCLSCSSSCTTDQVEIRACTKQQNRVCACEAGRYCALKTHSGSCRQCMRLSKCGPGFGVASSRAPNGNVLCKACAPGTFSDTTSSTDVCRPHRICSILAIPGNASTDAVCAPESPTLSAIPRTLYVSQPEPTRSQPLDQEPGPSQTPSILTSLGSTPIIEQSTKGGISLPIGLIVGVTSLGLLMLGLVNCIILVQRKKKPSCLQRDAKVPHVPDEKSQDAVGLEQQHLLTTAPSSSSSSLESSASAGDRRAPPGGHPQARVMAEAQGFQEARASSRISDSSHGSHGTHVNVTCIVNVCSSSDHSSQCSSQASATVGDPDAKPSASPKDEQVPFSQEECPSQSPCETTETLQSHEKPLPLGVPDMGMKPSQAGWFDQIAVKVA traf1_mouse TNF receptor-associated factor 1 Traf1 protein protein mouse Mus musculus Mouse MASSSAPDENEFQFGCPPAPCQDPSEPRVLCCTACLSENLRDDEDRICPKCRADNLHPVSPGSPLTQEKVHSDVAEAEIMCPFAGVGCSFKGSPQSMQEHEATSQSSHLYLLLAVLKEWKSSPGSNLGSAPMALERNLSELQLQAAVEATGDLEVDCYRAPCCESQEELALQHLVKEKLLAQLEEKLRVFANIVAVLNKEVEASHLALAASIHQSQLDREHILSLEQRVVELQQTLAQKDQVLGKLEHSLRLMEEASFDGTFLWKITNVTKRCHESVCGRTVSLFSPAFYTAKYGYKLCLRLYLNGDGSGKKTHLSLFIVIMRGEYDALLPWPFRNKVTFMLLDQNNREHAIDAFRPDLSSASFQRPQSETNVASGCPLFFPLSKLQSPKHAYVKDDTMFLKCIVDTSA tnfr2-traf1 Directed yeast-2-hybrid reveals interaction between the Tumor necrosis factor receptor superfamily member 1B precursor (TNFR2) and TNF receptor associated factor 1 (TRAF1) 177877 N/A 177880 unspecified role unspecified role bait bait cytoplasmic domain cytoplasmic domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 177881 unspecified role unspecified role prey prey physical association physical association false false false Authors note that the interaction between TNF-R2 and TRAF1 was 'weak' indicating this association was indirect. Evidence from this study implcates TRAF2 in mediating this interaction. traf1-traf2-1 Directed yeast-2-hybrid reveals interaction between the TNF receptor associated factor 1 (TRAF1) and TNF receptor associated factor 2 (TRAF2) 177877 N/A 177881 unspecified role unspecified role bait bait N/A 177882 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false traf2-traf1 Directed yeast-2-hybrid reveals interaction between the TNF receptor associated factor 2 (TRAF2) and TNF receptor associated factor 1 (TRAF1) 177877 N/A 177882 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 177881 unspecified role unspecified role prey prey physical association physical association false false false traf2-traf2-2 Directed yeast-2-hybrid reveals self association of the TNF receptor associated factor 2 (TRAF2) protein 177877 N/A 177882 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 177882 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false traf1-traf1-1 Directed yeast-2-hybrid reveals self association of the TNF receptor associated factor 1 (TRAF1) protein 177877 N/A 177881 unspecified role unspecified role prey prey N/A 177881 unspecified role unspecified role bait bait physical association physical association false false false tnfr2-traf1-1 Directed yeast-2-hybrid reveals interaction between the Tumor necrosis factor receptor superfamily member 1B precursor (TNFR2) and TNF receptor associated factor 1 (TRAF1) 177877 N/A 177883 unspecified role unspecified role bait bait cytoplasmic domain cytoplasmic domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 177881 unspecified role unspecified role prey prey physical association physical association false false false Authors note that the interaction between TNF-R2 and TRAF1 was 'weak' indicating this association was indirect. Evidence from this study implcates TRAF2 in mediating this interaction. tnfr2-traf2-1 Directed yeast-2-hybrid reveals interaction between the Tumor necrosis factor receptor superfamily member 1B precursor (TNFR2) and TNF receptor associated factor 2 (TRAF2) 177877 N/A 177883 unspecified role unspecified role bait bait cytoplasmic domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 177882 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false tnfr2-traf2-2 Directed yeast-2-hybrid reveals interaction between the Tumor necrosis factor receptor superfamily member 1B precursor (TNFR2) and TNF receptor associated factor 2 (TRAF2) 177877 N/A 177880 unspecified role unspecified role bait bait cytoplasmic domain cytoplasmic domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 177882 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false tnfr2-traf2 Yeast-2-hybrid reveals interaction between the Tumor necrosis factor receptor superfamily member 1B precursor (TNFR2) and TNF receptor associated factor 2 (TRAF2) 177878 N/A 177882 unspecified role unspecified role prey prey N/A 177880 unspecified role unspecified role bait bait cytoplasmic domain cytoplasmic domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false tnfr2-traf1-2 GST pulldown reveals interaction between the the Tumor necrosis factor receptor superfamily member 1B precursor (TNF-R2) and the TNF receptor associated factor 1 (TRAF1) protein 177879 N/A 177880 unspecified role unspecified role bait bait cytoplasmic domain cytoplasmic domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 177884 unspecified role unspecified role prey prey mouse-ctll_2 Mus musculus CTLL-2 C57b1/6 T cell lymphoblasts ctll_2 CTLL-2 mouse C57b1/6 T cell lymphoblasts physical association physical association false false false