IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yan-2013-1 Inactivation of BAD by IKK inhibits TNFα-induced apoptosis independently of NF-κB activation. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yan J., Xiang J., Lin Y., Ma J., Zhang J., Zhang H., Sun J., Danial N.N., Liu J., Lin A. Apoptosis - Interactions involving proteins with a function related to apoptosis Interactions involving 14-3-3 proteins could not be curated as pan-14-3-3 antibodies were used. Interactions involving Ikbkb and IκBalpha also could not be curated as the origin of the constructs could not be traced. anninglin@bsd.uchicago.edu Cell (0092-8674) 2013 Only protein-protein interactions imex curation bad_mouse Bcl2-associated agonist of cell death Bad Bbc6 Bcl-2-binding component 6 Bcl-xL/Bcl-2-associated death promoter Bcl2 antagonist of cell death protein protein mouse Mus musculus Mouse MGTPKQPSLAPAHALGLRKSDPGIRSLGSDAGGRRWRPAAQSMFQIPEFEPSEQEDASATDRGLGPSLTEDQPGPYLAPGLLGSNIHQQGRAATNSHHGGAGAMETRSRHSSYPAGTEEDEGMEEELSPFRGRSRSAPPNLWAAQRYGRELRRMSDEFEGSFKGLPRPKSAGTATQMRQSAGWTRIIQSWWDRNLGKGGSTPSQ q64373-1 Bcl-2-like protein 1 Bcl2l1 Bcl2l Bclx Apoptosis regulator Bcl-X Bcl-xL protein protein mouse Mus musculus Mouse MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEAERETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK bcl2l1-bad-1 1391663 n/a 1391664 unspecified role unspecified role prey prey n/a 1391665 unspecified role unspecified role bait bait physical association physical association false false false Fig 5C, 5E Only nonphosphorylated, but not Ser26-phosphorylated, BAD was found to associate with BCL-XL TNF-alpha Interaction observed in Ikbkb -/- cells. bad-bcl2l1-3 1391663 n/a 1391665 unspecified role unspecified role prey prey n/a 1391664 unspecified role unspecified role bait bait physical association physical association false false false Supp. Fig. 4B Only nonphosphorylated, but not Ser26-phosphorylated, BAD was found to associate with BCL-XL. 14-3-3 proteins were also present in this interaction. Interaction observed in Ikbkb -/- cells. TNF-alpha