IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hopkins-2011-1 The impact of a novel apolipoprotein-E and amyloid-! precursor protein-interacting protein on the production of amyloid-B yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Apoptosis - Interactions involving proteins with a function related to apoptosis APOE3 and E4 are polymorphisms rather than splice variants. E3 is represented by the UniProt canonical sequence, E4 as a mutant of that sequence. 2011-05-04: P. Hopkins, Kings College London, MRC Centre for Neurodegeneration Research, Institute of Psychiatry, De Crespigny Park, London, UK. paul.hopkins@kcl.ac.uk Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease hopkins-2011-2 The impact of a novel apolipoprotein-E and amyloid-! precursor protein-interacting protein on the production of amyloid-B human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Apoptosis - Interactions involving proteins with a function related to apoptosis APOE3 and E4 are polymorphisms rather than splice variants. E3 is represented by the UniProt canonical sequence, E4 as a mutant of that sequence. 2011-05-04: P. Hopkins, Kings College London, MRC Centre for Neurodegeneration Research, Institute of Psychiatry, De Crespigny Park, London, UK. Source of APP traced to PMID:2881207 paul.hopkins@kcl.ac.uk Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease hopkins-2011-3 The impact of a novel apolipoprotein-E and amyloid-! precursor protein-interacting protein on the production of amyloid-B in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Apoptosis - Interactions involving proteins with a function related to apoptosis APOE3 and E4 are polymorphisms rather than splice variants. E3 is represented by the UniProt canonical sequence, E4 as a mutant of that sequence. 2011-05-04: P. Hopkins, Kings College London, MRC Centre for Neurodegeneration Research, Institute of Psychiatry, De Crespigny Park, London, UK. paul.hopkins@kcl.ac.uk Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease hopkins-2011-4 The impact of a novel apolipoprotein-E and amyloid-! precursor protein-interacting protein on the production of amyloid-B mouse-brain Mus musculus brain brain Brain [cerebrum] density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Apoptosis - Interactions involving proteins with a function related to apoptosis APOE3 and E4 are polymorphisms rather than splice variants. E3 is represented by the UniProt canonical sequence, E4 as a mutant of that sequence. 2011-05-04: P. Hopkins, Kings College London, MRC Centre for Neurodegeneration Research, Institute of Psychiatry, De Crespigny Park, London, UK. paul.hopkins@kcl.ac.uk Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease hopkins-2011-5 The impact of a novel apolipoprotein-E and amyloid-! precursor protein-interacting protein on the production of amyloid-B rat-brain Rattus norvegicus brain brain Brain [cerebrum] comigration in gel comigration in gel electrophoresis comigration in gel western blot western blot Immuno blot Immuno blot Apoptosis - Interactions involving proteins with a function related to apoptosis APOE3 and E4 are polymorphisms rather than splice variants. E3 is represented by the UniProt canonical sequence, E4 as a mutant of that sequence. 2011-05-04: P. Hopkins, Kings College London, MRC Centre for Neurodegeneration Research, Institute of Psychiatry, De Crespigny Park, London, UK. Western blot analysis of 2-dimensional blue-native/SDS-PAGE gels paul.hopkins@kcl.ac.uk Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease hopkins-2011-6 The impact of a novel apolipoprotein-E and amyloid-! precursor protein-interacting protein on the production of amyloid-B human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Apoptosis - Interactions involving proteins with a function related to apoptosis APOE3 and E4 are polymorphisms rather than splice variants. E3 is represented by the UniProt canonical sequence, E4 as a mutant of that sequence. 2011-05-04: P. Hopkins, Kings College London, MRC Centre for Neurodegeneration Research, Institute of Psychiatry, De Crespigny Park, London, UK. Source of APP traced to PMID:2881207 paul.hopkins@kcl.ac.uk Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease hopkins-2011-7 The impact of a novel apolipoprotein-E and amyloid-! precursor protein-interacting protein on the production of amyloid-B rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell confocal microscopy confocal microscopy immunostaining immunostaining Apoptosis - Interactions involving proteins with a function related to apoptosis APOE3 and E4 are polymorphisms rather than splice variants. E3 is represented by the UniProt canonical sequence, E4 as a mutant of that sequence. 2011-05-04: P. Hopkins, Kings College London, MRC Centre for Neurodegeneration Research, Institute of Psychiatry, De Crespigny Park, London, UK. Western blot analysis of 2-dimensional blue-native/SDS-PAGE gels paul.hopkins@kcl.ac.uk Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease tmcc2_human Transmembrane and coiled-coil domains protein 2 Cerebral protein 11 TMCC2 KIAA0481 hucep-11 protein protein human Homo sapiens Human MKRCRSDELQQQQGEEDGAGLEDAASHLPGADLRPGETTGANSAGGPTSDAGAAAAPNPGPRSKPPDLKKIQQLSEGSMFGHGLKHLFHSRRRSREREHQTSQDSQQHQQQQGMSDHDSPDEKERSPEMHRVSYAMSLHDLPARPTAFNRVLQQIRSRPSIKRGASLHSSSGGGSSGSSSRRTKSSSLEPQRGSPHLLRKAPQDSSLAAILHQHQCRPRSSSTTDTALLLADGSNVYLLAEEAEGIGDKVDKGDLVALSLPAGHGDTDGPISLDVPDGAPDPQRTKAAIDHLHQKILKITEQIKIEQEARDDNVAEYLKLANNADKQQVSRIKQVFEKKNQKSAQTIAQLHKKLEHYRRRLKEIEQNGPSRQPKDVLRDMQQGLKDVGANVRAGISGFGGGVVEGVKGSLSGLSQATHTAVVSKPREFASLIRNKFGSADNIAHLKDPLEDGPPEEAARALSGSATLVSSPKYGSDDECSSASASSAGAGSNSGAGPGGALGSPKSNALYGAPGNLDALLEELREIKEGQSHLEDSMEDLKTQLQRDYTYMTQCLQEERYRYERLEEQLNDLTELHQNEMTNLKQELASMEEKVAYQSYERARDIQEAVESCLTRVTKLELQQQQQQVVQLEGVENANARALLGKFINVILALMAVLLVFVSTIANFITPLMKTRLRITSTTLLVLVLFLLWKHWDSLTYLLEHVLLPS apoe_human Apolipoprotein E APOE protein protein human Homo sapiens Human MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH tmcc2_mouse Transmembrane and coiled-coil domains protein 2 Tmcc2 Kiaa0481 protein protein mouse Mus musculus Mouse MKRCKSDELQQQQGEEDGAGMEDAACLLPGADLRHGEASSANSAGGPTSDAGAAVAPNPGPRSKPPDLKKIQQLSEGSMFGHGLKHLFHSRRRSREREHQASQEAQQQQQQQGLSDQDSPDEKERSPEMHRVSYAVSLHDLPARPTAFNRVLQQIRSRPSIKRGASLHSSGGSGGRRAKSSSLEPQRGSPHLLRKAPQDSSLAAILHQHQGRPRSSSTTDTALLLADGSSAYLLAEEAESIGDKGDKGDLVALSLPSGPGHGDSDGPISLDVPDGAPDPQRTKAAIEHLHQKILKITEQIKIEQEARDDNVAEYLKLANNADKQQVSRIKQVFEKKNQKSAQTIAQLHKKLEHYRRRLKEIEQNGPSRQPKDVLRDMQQGLKDVGANMRAGISGFGGGVVEGVKGSLSGLSQATHTAVVSKPREFASLIRNKFGSADNIAHLKDPMEDGPPEEAARALSGSATLVSSPKYGSDDECSSASASSAGAGSNSGAGPGGALGSPRSNTLYGAPGNLDTLLEELREIKEGQSHLEDSMEDLKTQLQRDYTYMTQCLQEERYRYERLEEQLNDLTELHQNEMTNLKQELASMEEKVAYQSYERARDIQEAVESCLTRVTKLELQQQQQQVVQLEGVENANARALLGKFINVILALMAVLLVFVSTIANFITPLMKTRLRITSTALLLLVLFLLWKHWASLTYLLEHVLLPS apoe_mouse Apolipoprotein E Apoe protein protein mouse Mus musculus Mouse MKALWAVLLVTLLTGCLAEGEPEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQAFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFEPIVEDMHRQWANLMEKIQASVATNPIITPVAQENQ p08592-pro_0000000161 Soluble APP-beta App ABPP Alzheimer disease amyloid A4 protein homolog Amyloidogenic glycoprotein Amyloid precursor protein A4 AD1 Alzheimer disease amyloid protein Amyloid-beta (A4) precursor protein Amyloid-beta A4 protein protein protein rat Rattus norvegicus (Rat) Rat LEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSIDSADAEEDDSDVWWGGADTDYADGGEDKVVEVAEEEEVADVEEEEAEDDEDVEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSVSSQSLLKTTSEPLPQDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKM 18 671 d3ze26_rat Tmcc2 RGD1311960_predicted rCG_46440 protein protein rat Rattus norvegicus (Rat) Rat MKRCKSDELQQQQGEEDGAGMEDAACLLPGADLRHGEASSANSAGGPTSDAGAAVAPNPGPRSKPPDLKKIQQLSEGSMFGHGLKHLFHSRRRSREREHQASQEAQQQQQHQGLSDQDSPDEKERSPEMHRVSYAVSLHDLPARPTAFNRVLQQIRSRPSIKRGASLHSSGGSGGRRAKSSSLEPQRGSPHLLRKAPQDSSLTAILHQHQGRPRSSSTTDTALLLADGSNAYLLAEEAESTGDKGDKGDLVALSLPSGPGHGDTDGPISLDVPDGAPDPQRTKAAIDHLHQKILKITEQIKIEQEARDDNVAEYLKLANNADKQQVSRIKQVFEKKNQKSAQTIAQLHKKLEQYRRRLKEIEQNGPSRQPKDVLRDMQQGLKDVGANMRAGISGFGGGVVEGVKGSLSGLSQATHTAVVSKPREFASLIRNKFGSADNIAHLKDPMEDGPPEEAARALSGSATLVSSPKYGSDDECSSASASSAGAGSNSGAGPAGALGSPRSNTLYGAPGNLDTLLEELREIKDGQSHLEDSMEDLKTQLQRDYTYMTQCLQEERYRYERLEEQLNDLTELHQNEMTNLKQELASMEEKVAYQSYERARDIQEAVESCLTRVTKLELQQQQQQVVQLEGVENANARALLGKFINVILALMAVLLVFVSTIANFITPLMKTRLRITSTALLLLVLFLLWKHWDSLTYLLEHVLLPS p05067-pro_0000000091 C99 APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN 672 770 apoe-tmcc2-1 1144020 n/a 1144027 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1144028 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1A apoe-tmcc2-2 1144020 n/a 1144028 unspecified role unspecified role bait bait P02649:p.Cys130Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1144027 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A tmcc2-apoe 1144021 n/a 1144027 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal trxa fusion fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1144028 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1C tmcc2-apoe-1 1144021 n/a 1144028 unspecified role unspecified role unspecified role unspecified role P02649:p.Cys130Arg mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1144027 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal trxa fusion fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1C apoe-tmcc2-3 1144022 n/a 1144028 unspecified role unspecified role neutral component neutral component n/a 1144027 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal trxa fusion fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1C apoe-tmcc2-4 1144023 n/a 1144029 unspecified role unspecified role neutral component neutral component n/a 1144030 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 3A d3ze26-p08592_pro-2 1144024 n/a 1144031 unspecified role unspecified role neutral component neutral component n/a 1144032 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 4A, 4B p05067_pro-tmcc2-1 1144025 n/a 1144027 unspecified role unspecified role prey prey c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1144033 unspecified role unspecified role bait bait region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false d3ze26-p08592_pro-1 1144026 n/a 1144032 unspecified role unspecified role neutral component neutral component n/a 1144031 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4E