I2D I2D legros-2011-1 The HTLV-1 Tax protein inhibits formation of stress granules by interacting with histone deacetylase 6. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Legros S., Boxus M., Gatot J.S., Van Lint C., Kruys V., Kettmann R., Twizere J.C., Dequiedt F. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Flag-HDAC6 is human. fdequiedt@ulg.ac.be Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer legros-2011-4 The HTLV-1 Tax protein inhibits formation of stress granules by interacting with histone deacetylase 6. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Legros S., Boxus M., Gatot J.S., Van Lint C., Kruys V., Kettmann R., Twizere J.C., Dequiedt F. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Flag-HDAC6 is human. fdequiedt@ulg.ac.be Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer legros-2011-2 The HTLV-1 Tax protein inhibits formation of stress granules by interacting with histone deacetylase 6. human-mt2 Homo sapiens T cell, HTLV-1 immortalized mt2 Human T cell, lymphoblast, Human T Lymphotrohpic Virus 1 (HTLV-1) immortalized confocal microscopy confocal microscopy immunostaining immunostaining Legros S., Boxus M., Gatot J.S., Van Lint C., Kruys V., Kettmann R., Twizere J.C., Dequiedt F. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Flag-HDAC6 is human. fdequiedt@ulg.ac.be Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer legros-2011-3 The HTLV-1 Tax protein inhibits formation of stress granules by interacting with histone deacetylase 6. human-slb-1 Homo sapiens T cell, HTLV-1 immortalized slb-1 Homo sapiens T cell, HTLV-1 immortalized confocal microscopy confocal microscopy immunostaining immunostaining Legros S., Boxus M., Gatot J.S., Van Lint C., Kruys V., Kettmann R., Twizere J.C., Dequiedt F. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Flag-HDAC6 is human. fdequiedt@ulg.ac.be Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer legros-2011-5 The HTLV-1 Tax protein inhibits formation of stress granules by interacting with histone deacetylase 6. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Legros S., Boxus M., Gatot J.S., Van Lint C., Kruys V., Kettmann R., Twizere J.C., Dequiedt F. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Flag-HDAC6 is human. fdequiedt@ulg.ac.be Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer tiar_human Nucleolysin TIAR TIAL1 TIA-1-related protein protein protein human Homo sapiens Human MMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEHTSNDPYCFVEFYEHRDAAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPFGKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAPKSTQENNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVYGNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIPPPNQAGYGMASYQTQ tax_htl1a Protein Tax-1 Tax Protein X-LOR Trans-activating transcriptional regulatory protein of HTLV-1 protein protein htl1a Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) MAHFPGFGQSLLFGYPVYVFGDCVQGDWCPISGGLCSARLHRHALLATCPEHQITWDPIDGRVIGSALQFLIPRLPSFPTQRTSKTLKVLTPPITHTTPNIPPSFLQAMRKYSPFRNGYMEPTLGQHLPTLSFPDPGLRPQNLYTLWGGSVVCMYLYQLSPPITWPLLPHVIFCHPGQLGAFLTNVPYKRIEELLYKISLTTGALIILPEDCLPTTLFQPARAPVTLTAWQNGLLPFHSTLTTPGLIWTFTDGTPMISGPCPKDGQPSLVLQSSSFIFHKFQTKAYHPSFLLSHGLIQYSSFHSLHLLFEEYTNIPISLLFNEKEADDNDHEPQISPGGLEPPSEKHFRETEV g3bp1_human Ras GTPase-activating protein-binding protein 1 G3BP1 G3BP ATP-dependent DNA helicase VIII GAP SH3 domain-binding protein 1 protein protein human Homo sapiens Human MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ hdac6_human Histone deacetylase 6 HDAC6 KIAA0901 JM21 Tubulin-lysine deacetylase HDAC6 protein protein human Homo sapiens Human MTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDEQLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKEELMLVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASGSVLRLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQQKHRIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKASNWSTTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLVLVAAGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLRALAEGVSASLHTLLGDPCPMLESPGAPCRSAQASVSCALEALEPFWEVLVRSTETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNMMNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRLVEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGHALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKVEDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGEESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTIQENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAYVHHQALLDVKNIAHQNKFGEDMPHPH tax-tial1-1 1231812 n/a 1231817 unspecified role unspecified role neutral component neutral component n/a 1231818 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1e g3bp1-tax 1231812 n/a 1231819 unspecified role unspecified role neutral component neutral component n/a 1231818 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 1e, f, 2a, g, 5f, S2 Cells were either treated with 1mM Aresenite or Sorbitol (600mM, 2hrs) or heat shock (43 degrees C, 50 min). g3bp1-hdac6 1231812 n/a 1231820 unspecified role unspecified role neutral component neutral component n/a 1231818 unspecified role unspecified role neutral component neutral component n/a 1231819 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4c hdac6-tax-1 1231812 n/a 1231818 unspecified role unspecified role neutral component neutral component n/a 1231820 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4g Cells were treated with 1mM arsenite for 30 mins. tax-hdac6 1231813 n/a 1231820 unspecified role unspecified role prey prey n/a 1231818 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4a hdac6-tax 1231813 n/a 1231820 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 1231818 unspecified role unspecified role prey prey physical association physical association false false false 4b, f g3bp1-tax-1 1231814 n/a 1231818 unspecified role unspecified role neutral component neutral component n/a 1231819 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1g g3bp1-tax-2 1231815 n/a 1231818 unspecified role unspecified role neutral component neutral component n/a 1231819 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1g hdac6-tax-2 1231816 n/a 1231820 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1231818 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5d