IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zinzalla-2011-2 Activation of mTORC2 by association with the ribosome. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation zinzalla-2011-3 Activation of mTORC2 by association with the ribosome. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis 15%–45% sucrose gradient m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation zinzalla-2011-1 Activation of mTORC2 by association with the ribosome. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation zinzalla-2011-4 Activation of mTORC2 by association with the ribosome. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation zinzalla-2011-5 Activation of mTORC2 by association with the ribosome. human-na8 Homo sapiens na8 Human melanoma cell line NA8 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis 15%–45% sucrose gradient m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation zinzalla-2011-6 Activation of mTORC2 by association with the ribosome. human-juso Homo sapiens juso Human melanoma cell line Juso density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis 15%–45% sucrose gradient m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation zinzalla-2011-7 Activation of mTORC2 by association with the ribosome. human-me67 Homo sapiens me67 Human melanom cell line ME67 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis 15%–45% sucrose gradient m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation zinzalla-2011-8 Activation of mTORC2 by association with the ribosome. human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis 15%–45% sucrose gradient m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation zinzalla-2011-9 Activation of mTORC2 by association with the ribosome. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis 15%–45% sucrose gradient m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation zinzalla-2011-10 Activation of mTORC2 by association with the ribosome. human-sw620 Human caucasian colon adenocarcinoma SW620 cells sw620 Human caucasian colon adenocarcinoma SW620 cells density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis 15%–45% sucrose gradient m.hall@unibas.ch Cell (0092-8674) 2011 Only protein-protein interactions imex curation sin1_human Target of rapamycin complex 2 subunit MAPKAP1 MIP1 Mitogen-activated protein kinase 2-associated protein 1 Stress-activated map kinase-interacting protein 1 MAPKAP1 SIN1 protein protein human Homo sapiens Human MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQGSNGETQGYVYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGPQYRLEKQSEPNVAVDLDSTLESQSAWEFCLVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADYFAQKQRKLNRRTSFSFQKEKKSGQQ lst8_human Target of rapamycin complex subunit LST8 MLST8 GBL G protein beta subunit-like LST8 Mammalian lethal with SEC13 protein 8 protein protein human Homo sapiens Human MNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEVTPDRSMIAAAGYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMYTGGEDCTARIWDLRSRNLQCQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSITSAHIDPDASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSADQTCKIWRTSNFSLMTELSIKSGNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVETGEIKREYGGHQKAVVCLAFNDSVLG rictr_human Rapamycin-insensitive companion of mTOR RICTOR KIAA1999 AVO3 homolog protein protein human Homo sapiens Human MAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVARLQGVSNMRKLGHLNNFTKLLCDIGHSEEKLGFHYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITVNASLFPSSVTNSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNTILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREARFLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTEEFIEALLSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHISVRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLKRFHEMKKRGPKPYSLHLDHIIQKAIATHQKRDQYLRVQKDIFILKDTEEALLINLRDSQVLQHKENLEWNWNLIGTILKWPNVNLRNYKDEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDIVQWLNASSGMKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLLKLTVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEALDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHREYNSKYVDLIEEQLNEALTTYRKPVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQNIITELCRNVRTPDLDKWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQGCDILKCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPSTLSLNSESTSSRHNSESESVPSSMFILEDDRFGSSSTSTFFLDINEDTEPTFYDRSGPIKDKNSFPFFASSKLVKNRILNSLTLPNKKHRSSSDPKGGKLSSESKTSNRRIRTLTEPSVDFNHSDDFTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFGTENHRENTSRERLVVESSTSSHMKIRSQSFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVSTKTIKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFSYTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINQNTLQRSSSVRSMVSSATYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGGLPSGTGGLVKNSFHLLRQQMSLTEIMNSIHSDASLFLESTEDTGLQEHTDDNCLYCVCIEILGFQPSNQLSAICSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQEGSASSTKSTELLLGVKTIPDDTPMCRILLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEVSHLLSHCTFRLPCRRFIQELFQDVQFLQMHEEAEAVLATPPKQPIVDTSAES mtor_human Serine/threonine-protein kinase mTOR MTOR FRAP FRAP2 FK506-binding protein 12-rapamycin complex-associated protein 1 Rapamycin target protein 1 FRAP1 Mammalian target of rapamycin FKBP12-rapamycin complex-associated protein Mechanistic target of rapamycin RAFT1 RAPT1 Rapamycin and FKBP12 target 1 protein protein human Homo sapiens Human MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLIKQATSHENLCQCYIGWCPFW rl26_human 60S ribosomal protein L26 RPL26 Large ribosomal subunit protein uL24 protein protein human Homo sapiens Human MKFNPFVTSDRSKNRKRHFNAPSHIRRKIMSSPLSKELRQKYNVRSMPIRKDDEVQVVRGHYKGQQIGKVVQVYRKKYVIYIERVQREKANGTTVHVGIHPSKVVITRLKLDKDRKKILERKAKSRQVGKEKGKYKEETIEKMQE rl7_human 60S ribosomal protein L7 RPL7 Large ribosomal subunit protein uL30 protein protein human Homo sapiens Human MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN rl17_human 60S ribosomal protein L17 60S ribosomal protein L23 PD-1 RPL17 Large ribosomal subunit protein uL22 protein protein human Homo sapiens Human MVRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDVTLQKQCVPFRRYNGGVGRCAQAKQWGWTQGRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKEQIVPKPEEEVAQKKKISQKKLKKQKLMARE rs16_human 40S ribosomal protein S16 RPS16 Small ribosomal subunit protein uS9 protein protein human Homo sapiens Human MPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR ypk2_yeast Serine/threonine-protein kinase YPK2/YKR2 YPK2 YKR2 YMR104C YM9718.03C protein protein yeast Saccharomyces cerevisiae Baker's yeast MHSWRISKFKLGRSKEDDGSSEDENEKSWGNGLFHFHHGEKHHDGSPKNHNHEHEHHIRKINTNETLPSSLSSPKLRNDASFKNPSGIGNDNSKASERKASQSSTETQGPSSESGLMTVKVYSGKDFTLPFPITSNSTILQKLLSSGILTSSSNDASEVAAIMRQLPRYKRVDQDSAGEGLIDRAFATKFIPSSILLPGSTNSSPLLYFTIEFDNSITTISPDMGTMEQPVFNKISTFDVTRKLRFLKIDVFARIPSLLLPSKNWQQEIGEQDEVLKEILKKINTNQDIHLDSFHLPLNLKIDSAAQIRLYNHHWISLERGYGKLNITVDYKPSKNKPLSIDDFDLLKVIGKGSFGKVMQVRKKDTQKIYALKALRKAYIVSKCEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLYLVLAFINGGELFYHLQHEGRFSLARSRFYIAELLCALDSLHKLDVIYRDLKPENILLDYQGHIALCDFGLCKLNMKDNDKTDTFCGTPEYLAPEILLGQGYTKTVDWWTLGILLYEMMTGLPPYYDENVPVMYKKILQQPLLFPDGFDPAAKDLLIGLLSRDPSRRLGVNGTDEIRNHPFFKDISWKKLLLKGYIPPYKPIVKSEIDTANFDQEFTKEKPIDSVVDEYLSASIQKQFGGWTYIGDEQLGDSPSQGRSIS E1FAB0295386A113 crosstide Crosstide Ser/Thr protein kinase substrate peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) GRPRTSSFAEG dt-dna oligo(dT) dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR rictor-mapkap1 1157775 n/a 1157785 unspecified role unspecified role prey prey n/a 1157786 unspecified role unspecified role prey prey n/a 1157787 unspecified role unspecified role bait bait n/a 1157788 unspecified role unspecified role prey prey association association false false false Fig. 2B, 3B. rpl26-mtor 1157775 n/a 1157789 unspecified role unspecified role bait bait n/a 1157787 unspecified role unspecified role prey prey n/a 1157788 unspecified role unspecified role prey prey association association false false false Supp. Fig. 5C, S6C, S6D. Fig 6A, 6B, 6C. Inhibition of PI3K strongly decreased mTORC2/Rictor-Rpl26 interaction, conversely hyperactivation of PI3K signaling increased the amount of mTOR and rictor in Rpl26 immunoprecipitates, Insulin stimulates mTORC2-ribosome association. Insulin, PTEN siRNA LY294002 rpl26-mapkap1 1157775 n/a 1157788 unspecified role unspecified role prey prey n/a 1157789 unspecified role unspecified role bait bait n/a 1157787 unspecified role unspecified role prey prey n/a 1157785 unspecified role unspecified role prey prey n/a 1157790 unspecified role unspecified role prey prey association association false false false Fig. 5A rpl26-mtor-2 1157775 n/a 1157785 unspecified role unspecified role ancillary ancillary n/a 1157789 unspecified role unspecified role bait bait n/a 1157788 unspecified role unspecified role prey prey association association false false false Supp. Fig. 6A Inhibition of PI3K strongly decreased mTORC2/Rictor-Rpl26 interaction, conversely hyperactivation of PI3K signaling increased the amount of mTOR and rictor in Rpl26 immunoprecipitates, Insulin stimulates mTORC2-ribosome association. rpl26-mtor-5 1157775 n/a 1157791 unspecified role unspecified role ancillary ancillary n/a 1157787 unspecified role unspecified role prey prey n/a 1157792 unspecified role unspecified role ancillary ancillary n/a 1157789 unspecified role unspecified role bait bait n/a 1157788 unspecified role unspecified role prey prey association association false false false Supp. Fig. 6B mapkap1-mtor-1 1157776 n/a 1157789 unspecified role unspecified role neutral component neutral component n/a 1157792 unspecified role unspecified role neutral component neutral component n/a 1157785 unspecified role unspecified role neutral component neutral component n/a 1157787 unspecified role unspecified role neutral component neutral component n/a 1157790 unspecified role unspecified role neutral component neutral component n/a 1157788 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp. Fig. 5A mapkap1-mtor-2 1157776 n/a 1157790 unspecified role unspecified role neutral component neutral component n/a 1157792 unspecified role unspecified role neutral component neutral component n/a 1157787 unspecified role unspecified role neutral component neutral component n/a 1157789 unspecified role unspecified role neutral component neutral component n/a 1157788 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 5B crosstide-ypk2 1157777 n/a 1157793 enzyme enzyme unspecified role unspecified role region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 1157794 enzyme target enzyme target substrate substrate unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation false false false Fig. 1C dt_dna-gnb2l1 1157778 n/a 1157790 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1157787 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1157795 unspecified role unspecified role bait bait human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1157788 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1157789 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 n/a 1157796 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 association association false false false Supp Fig. 5B Binding assumed to be via Poly(A) mRNA-bound to ribosomes RNAse A gnb2l1-mapkap1 1157779 n/a 1157788 unspecified role unspecified role neutral component neutral component n/a 1157790 unspecified role unspecified role neutral component neutral component n/a 1157787 unspecified role unspecified role neutral component neutral component n/a 1157789 unspecified role unspecified role neutral component neutral component n/a 1157785 unspecified role unspecified role neutral component neutral component n/a 1157796 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 7B gnb2l1-mapkap1-1 1157780 n/a 1157789 unspecified role unspecified role neutral component neutral component n/a 1157790 unspecified role unspecified role neutral component neutral component n/a 1157796 unspecified role unspecified role neutral component neutral component n/a 1157787 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 7B gnb2l1-mapkap1-3 1157781 n/a 1157790 unspecified role unspecified role neutral component neutral component n/a 1157788 unspecified role unspecified role neutral component neutral component n/a 1157796 unspecified role unspecified role neutral component neutral component n/a 1157787 unspecified role unspecified role neutral component neutral component n/a 1157789 unspecified role unspecified role neutral component neutral component n/a 1157785 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 7B gnb2l1-mapkap1-3 1157782 n/a 1157789 unspecified role unspecified role neutral component neutral component n/a 1157785 unspecified role unspecified role neutral component neutral component n/a 1157796 unspecified role unspecified role neutral component neutral component n/a 1157787 unspecified role unspecified role neutral component neutral component n/a 1157788 unspecified role unspecified role neutral component neutral component n/a 1157790 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. S7A gnb2l1-mapkap1-6 1157783 n/a 1157785 unspecified role unspecified role neutral component neutral component n/a 1157788 unspecified role unspecified role neutral component neutral component n/a 1157787 unspecified role unspecified role neutral component neutral component n/a 1157796 unspecified role unspecified role neutral component neutral component n/a 1157789 unspecified role unspecified role neutral component neutral component n/a 1157790 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp. Fig. 7A gnb2l1-mapkap1-5 1157784 n/a 1157796 unspecified role unspecified role neutral component neutral component n/a 1157790 unspecified role unspecified role neutral component neutral component n/a 1157789 unspecified role unspecified role neutral component neutral component n/a 1157787 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp. Fig. 7A