IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ guenebeaud-2010-3 The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Guenebeaud C., Goldschneider D., Castets M., Guix C., Chazot G., Delloye-Bourgeois C., Eisenberg-Lerner A., Shohat G., Zhang M., Laudet V., Kimchi A., Bernet A., Mehlen P. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of constructs inferred from author's email. Identity of CIP2-A-flag construct used in figure 6a could not be inferred as it was a gift from another group and it was constructed from a cDNA clone whose origin could not be determined (clone ID: DKFZp762J0112Q3). mehlen@lyon.fnclcc.fr Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation guenebeaud-2010-4 The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guenebeaud C., Goldschneider D., Castets M., Guix C., Chazot G., Delloye-Bourgeois C., Eisenberg-Lerner A., Shohat G., Zhang M., Laudet V., Kimchi A., Bernet A., Mehlen P. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of constructs inferred from author's email. Identity of CIP2-A-flag construct used in figure 6a could not be inferred as it was a gift from another group and it was constructed from a cDNA clone whose origin could not be determined (clone ID: DKFZp762J0112Q3). mehlen@lyon.fnclcc.fr Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation guenebeaud-2010-1 The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Guenebeaud C., Goldschneider D., Castets M., Guix C., Chazot G., Delloye-Bourgeois C., Eisenberg-Lerner A., Shohat G., Zhang M., Laudet V., Kimchi A., Bernet A., Mehlen P. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of constructs inferred from author's email. Identity of CIP2-A-flag construct used in figure 6a could not be inferred as it was a gift from another group and it was constructed from a cDNA clone whose origin could not be determined (clone ID: DKFZp762J0112Q3). mehlen@lyon.fnclcc.fr Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation guenebeaud-2010-2 The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunostaining immunostaining Guenebeaud C., Goldschneider D., Castets M., Guix C., Chazot G., Delloye-Bourgeois C., Eisenberg-Lerner A., Shohat G., Zhang M., Laudet V., Kimchi A., Bernet A., Mehlen P. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of constructs inferred from author's email. Identity of CIP2-A-flag construct used in figure 6a could not be inferred as it was a gift from another group and it was constructed from a cDNA clone whose origin could not be determined (clone ID: DKFZp762J0112Q3). mehlen@lyon.fnclcc.fr Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation guenebeaud-2010-5 The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Guenebeaud C., Goldschneider D., Castets M., Guix C., Chazot G., Delloye-Bourgeois C., Eisenberg-Lerner A., Shohat G., Zhang M., Laudet V., Kimchi A., Bernet A., Mehlen P. Apoptosis - Interactions involving proteins with a function related to apoptosis Low-density detergent-insoluble membranes were fractionated by a discontinuous sucrose density gradient centrifugation, fractions were collected and separated by SDS-PAGE, and endogenous proteins were detected by western blot. Identity of constructs inferred from author's email. Identity of CIP2-A-flag construct used in figure 6a could not be inferred as it was a gift from another group and it was constructed from a cDNA clone whose origin could not be determined (clone ID: DKFZp762J0112Q3). mehlen@lyon.fnclcc.fr Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation guenebeaud-2010-8 The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guenebeaud C., Goldschneider D., Castets M., Guix C., Chazot G., Delloye-Bourgeois C., Eisenberg-Lerner A., Shohat G., Zhang M., Laudet V., Kimchi A., Bernet A., Mehlen P. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of constructs inferred from author's email. Identity of CIP2-A-flag construct used in figure 6a could not be inferred as it was a gift from another group and it was constructed from a cDNA clone whose origin could not be determined (clone ID: DKFZp762J0112Q3). mehlen@lyon.fnclcc.fr Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation guenebeaud-2010-6 The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Guenebeaud C., Goldschneider D., Castets M., Guix C., Chazot G., Delloye-Bourgeois C., Eisenberg-Lerner A., Shohat G., Zhang M., Laudet V., Kimchi A., Bernet A., Mehlen P. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of constructs inferred from author's email. Identity of CIP2-A-flag construct used in figure 6a could not be inferred as it was a gift from another group and it was constructed from a cDNA clone whose origin could not be determined (clone ID: DKFZp762J0112Q3). mehlen@lyon.fnclcc.fr Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation 2aab_human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform PPP2R1B PP2A subunit A isoform PR65-beta PP2A subunit A isoform R1-beta protein protein human Homo sapiens Human MAGASELGTGPGAAGGDGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGNFTGLVGGPDFAHCLLPPLENLATVEETVVRDKAVESLRQISQEHTPVALEAYFVPLVKRLASGDWFTSRTSACGLFSVCYPRASNAVKAEIRQQFRSLCSDDTPMVRRAAASKLGEFAKVLELDSVKSEIVPLFTSLASDEQDSVRLLAVEACVSIAQLLSQDDLETLVMPTLRQAAEDKSWRVRYMVADRFSELQKAMGPKITLNDLIPAFQNLLKDCEAEVRAAAAHKVKELGENLPIEDRETIIMNQILPYIKELVSDTNQHVKSALASVIMGLSTILGKENTIEHLLPLFLAQLKDECPDVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWAQNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGQEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIGPILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVLALA unc5b_rat Netrin receptor UNC5B Unc5b Unc5h2 Protein unc-5 homolog 2 Protein unc-5 homolog B protein protein rat Rattus norvegicus (Rat) Rat MRARSGARGALLLALLLCWDPTPSLAGIDSGGQALPDSFPSAPAEQLPHFLLEPEDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQKGHVTQESLDEATGLRIREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQACQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSRPLEPSGDVALYAGLVVAVFVVLAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYDSSTIGSGAGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLTIPGTGVSLLVPNGAIPQGKFYDLYLRINKTESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFTGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPAALKEVLELERTLGGYLVEEPKTLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQKALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAATTQLGPYAFKIPLSIRQKICNSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMTTDGDC dapk3_rat Death-associated protein kinase 3 Dapk3 Zipk DAP-like kinase ZIP-kinase MYPT1 kinase protein protein rat Rattus norvegicus (Rat) Rat MSTFRQEDVEDHYEMGEELGSGQFAIVRKCQQKGTGMEYAAKFIKKRRLPSSRRGVSREEIEREVSILREIRHPNIITLHDVFENKTDVVLILELVSGGELFDFLAEKESLTEDEATQFLKQILDGVHYLHSKRIAHFDLKPENIMLLDKHAASPRIKLIDFGIAHRIEAGSEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSSTSELAKDFIRRLLVKDPKRRMTIAQSLEHSWIKVRRREDGARKPERRRLRAARLREYSLKSHSSMPRNTSYASFERFSRVLEDVAAAEQGLRELQRGRRQCRERVCALRVAAEQREARCRDGSAGLGRDLRRLRTELGRTEALRTRAQEEARAALLGAGGLKRRLCRLENRYDALAAQVAAEVQFVRDLVRALEQERLQAECGVR unc5b_mouse Netrin receptor UNC5B Unc5b Unc5h2 Protein unc-5 homolog 2 Protein unc-5 homolog B protein protein mouse Mus musculus Mouse MRARSGVRSALLLALLLCWDPTPSLAGVDSAGQVLPDSYPSAPAEQLPYFLLEPQDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQNDHVTQESLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSHPLETSGDVALYAGLVVAVFVVVAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPNNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYNSSTIGSGSGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLSLPGTGVSLLVPNGAIPQGKFYDLYLHINKAESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFMGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQRALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAITTQLGPYAFKIPLSIRQKICSSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMATDGDC 2aab_mouse Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform Ppp2r1b PP2A subunit A isoform PR65-beta PP2A subunit A isoform R1-beta protein protein mouse Mus musculus Mouse MAGAAGPGSGPGAAGGDGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRTELLPFLTDTIYDEDEVLLALAEQLGNFTGLVGGPDFAHCLLPPLESLATVEETVVRDKAVESLRQISQEHTPVALEAHFVPLVKRLASGDWFTSRTSACGLFSVCYPRASNAVKAEIRQHFRSLCSDDTPMVRRAAASKLGEFAKVLELDSVKTEIVPLFTNLASDEQDSVRLLAVEACVSIAQLLSQEDLEALVMPTLRQAAEDKSWRVRYMVADKFSELQKAVGPKIALSDLIPAFQSLLRDCEAEVRAAAAHKVRELCENLPAEGRETVIMNQILPYIKELVSDTNQHVKSALASVIMGLSTVLGKENTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWAQNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGKEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIGPILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVLALA dapk1_human Death-associated protein kinase 1 DAPK1 DAPK protein protein human Homo sapiens Human MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGLQHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDVQDKGGSNAVYWAARHGHVDTLKFLSENKCPLDVKDKSGEMALHVAARYGHADVAQLLCSFGSNPNIQDKEEETPLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNACDKDGHIALHLAVRRCQMEVIKTLLSQGCFVDYQDRHGNTPLHVACKDGNMPIVVALCEANCNLDISNKYGRTPLHLAANNGILDVVRYLCLMGASVEALTTDGKTAEDLARSEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRRPRLSSTNSSRFPPSPLASKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAPTHHPHCSADDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVVVFSLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLQVVLVATHADIMNVPRPAGGEFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCPPMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEEDLRRIAQQLHSTGEINIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTVEDIQRLVPDSDVEELLQILDAMDICARDLSSGTMVDVPALIKTDNLHRSWADEEDEVMVYGGVRIVPVEHLTPFPCGIFHKVQVNLCRWIHQQSTEGDADIRLWVNGCKLANRGAELLVLLVNHGQGIEVQVRGLETEKIKCCLLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRAQTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPPDPLGKDWCLLAMNLGLPDLVAKYNTSNGAPKDFLPSPLHALLREWTTYPESTVGTLMSKLRELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR unc5b-ppp2r1b-3 1180748 n/a 1180755 unspecified role unspecified role prey prey n/a 1180756 unspecified role unspecified role bait bait intracellular domain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false physical association physical association false false false 3c The Presence of netrin-1 inhibits this interaction. unc5b-dapk3 1180748 n/a 1180756 unspecified role unspecified role bait bait intracellular domain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false n/a 1180757 unspecified role unspecified role prey prey physical association physical association false false false 3c The Presence of netrin-1 inhibits this interaction. ppp2r1b-unc5b 1180749 n/a 1180758 unspecified role unspecified role prey prey n/a 1180759 unspecified role unspecified role bait bait physical association physical association false false false 3d The antibodies used in this experiment don't allow to distinguish between endogenous ppp2r1b and ppp2r1a. The interaction was annotated to ppp2r1b based on results shown in figure 3a (EBI-4404209), in which detection with ppp2r1a-specific antibodies show that the alpha isoform doesn't interact with unc5b. unc5b-ppp2r1b 1180749 n/a 1180759 unspecified role unspecified role prey prey n/a 1180758 unspecified role unspecified role bait bait physical association physical association false false false 3d The antibodies used in this experiment do not allow the user to distinguish between endogenous ppp2r1b and ppp2r1a. The interaction was annotated to ppp2r1b based on results shown in figure 3a (EBI-4404209), in which detection with ppp2r1a-specific antibodies show that the alpha isoform does not interact with unc5b. unc5b-ppp2r1b-1 1180750 n/a 1180755 unspecified role unspecified role prey prey n/a 1180756 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3a The Presence of netrin-1 inhibits this interaction. The antibodies used in this experiment do not allow the user to distinguish between different isoforms of the regulatory subunit of ppp2a. The interaction was annotated to ppp2r1b because detection with ppp2r1a-specific antibodies show that the alpha isoform does not interact with unc5b. dapk3-ppp2r1b-2 1180751 n/a 1180755 unspecified role unspecified role neutral component neutral component n/a 1180757 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4a The presence of netrin-1 inhibits this interaction. The antibodies used in this experiment do not allow the user to distinguish between different isoforms of the regulatory subunit of ppp2a. The interaction was annotated to ppp2r1b following the author's line of reasoning, by which dapk and ppp2r1b are interacting as parts of a complex with unc5b, as shown in subsequent experiments, and whose formation is inhibited by netrin-1 binding to unc5b. dapk1-2aab 1180752 n/a 1180760 unspecified role unspecified role neutral component neutral component n/a 1180755 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4b The antibodies used in this experiment do not allow the user to distinguish between endogenous ppp2r1b and ppp2r1a. The interaction was annotated to ppp2r1b based in the results shown in figure 3a (EBI-4404209), in which detection with ppp2r1a-specific antibodies show that the alpha isoform does not interact with unc5b. ppp2r1b-dapk3 1180753 n/a 1180756 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O08722:p.Val619Gln mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1180755 unspecified role unspecified role bait bait n/a 1180757 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4g The antibodies used in this experiment do not allow the user to distinguish between different isoforms of the regulatory subunit of ppp2a. The interaction was annotated to ppp2r1b based on results shown in figure 3a (EBI-4404209), in which detection with ppp2r1a-specific antibodies show that the alpha isoform does not interact with unc5b. dapk3-ppp2r1b-1 1180754 n/a 1180757 unspecified role unspecified role neutral component neutral component flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1180755 unspecified role unspecified role neutral component neutral component n/a 1180756 unspecified role unspecified role neutral component neutral component ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4f The antibodies used in this experiment do not allow the user to distinguish between different isoforms of the regulatory subunit of ppp2a. The interaction was annotated to ppp2r1b based on results shown in figure 3a (EBI-4404209), in which detection with ppp2r1a-specific antibodies show that the alpha isoform does not interact with unc5b Netrin-1 can disrupt the interaction. When a mutant form of unc5h2 (v619Q) the interaction takes place regardless of the presence of netrin-1. This mutation keeps the intracellular domain of unc5b constitutively open, so it's apoptosis-inducing action can take place in the presence of netrin-1.