IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tan-2010-1 B55?-associated PP2A complex controls PDK1-directed myc signaling and modulates rapamycin sensitivity in colorectal cancer. human-dld1 Human colorectal adenocarcinoma dld1 Human colon adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tan J., Lee PL., Li Z., Jiang X., Lim YC., Hooi SC., Yu Q. Apoptosis - Interactions involving proteins with a function related to apoptosis Authors confirm that PPP2RB coding region was obtained from Human colon tissue. Interactions containing PPP2A C have not been included as this molecule cannot be uniquely identified. yuq@gis.a-star.edu.sg Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer Chromatin - Epigenetic interactions resulting in chromatin modulation tan-2010-2 B55?-associated PP2A complex controls PDK1-directed myc signaling and modulates rapamycin sensitivity in colorectal cancer. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tan J., Lee PL., Li Z., Jiang X., Lim YC., Hooi SC., Yu Q. Apoptosis - Interactions involving proteins with a function related to apoptosis Authors confirm that PPP2RB coding region was obtained from Human colon tissue. Interactions containing PPP2A C have not been included as this molecule cannot be uniquely identified. yuq@gis.a-star.edu.sg Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer Chromatin - Epigenetic interactions resulting in chromatin modulation tan-2010-4 B55?-associated PP2A complex controls PDK1-directed myc signaling and modulates rapamycin sensitivity in colorectal cancer. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tan J., Lee PL., Li Z., Jiang X., Lim YC., Hooi SC., Yu Q. Apoptosis - Interactions involving proteins with a function related to apoptosis HEK-TERV cells were used, which express both SV40 LT and mutant H-Ras-V12; they have constitutive activation of the MAPK-ERK pathway even in the absence of SV40 ST. Authors confirm that PPP2RB coding region was obtained from Human colon tissue. Interactions containing PPP2A C have not been included as this molecule cannot be uniquely identified. yuq@gis.a-star.edu.sg Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer Chromatin - Epigenetic interactions resulting in chromatin modulation tan-2010-3 B55?-associated PP2A complex controls PDK1-directed myc signaling and modulates rapamycin sensitivity in colorectal cancer. human-dld1 Human colorectal adenocarcinoma dld1 Human colon adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tan J., Lee PL., Li Z., Jiang X., Lim YC., Hooi SC., Yu Q. Apoptosis - Interactions involving proteins with a function related to apoptosis Authors confirm that PPP2RB coding region was obtained from Human colon tissue. Interactions containing PPP2A C have not been included as this molecule cannot be uniquely identified. yuq@gis.a-star.edu.sg Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer Chromatin - Epigenetic interactions resulting in chromatin modulation tan-2010-6 B55?-associated PP2A complex controls PDK1-directed myc signaling and modulates rapamycin sensitivity in colorectal cancer. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tan J., Lee PL., Li Z., Jiang X., Lim YC., Hooi SC., Yu Q. Apoptosis - Interactions involving proteins with a function related to apoptosis HEK-TERV cells were used, which express both SV40 LT and mutant H-Ras-V12; they have constitutive activation of the MAPK-ERK pathway even in the absence of SV40 ST. Authors confirm that PPP2RB coding region was obtained from Human colon tissue. Interactions containing PPP2A C have not been included as this molecule cannot be uniquely identified. yuq@gis.a-star.edu.sg Cancer Cell (1535-6108) 2010 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer Chromatin - Epigenetic interactions resulting in chromatin modulation 2abb_human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform PP2A subunit B isoform beta PP2A subunit B isoform B55-beta PP2A subunit B isoform PR55-beta PPP2R2B PP2A subunit B isoform R2-beta protein protein human Homo sapiens Human MEEDIDTRKINNSFLRDHSYATEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEIISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPSENIIAVAATNNLYIFQDKVN 2aaa_human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform PPP2R1A PP2A subunit A isoform PR65-alpha PP2A subunit A isoform R1-alpha Medium tumor antigen-associated 61 kDa protein protein protein human Homo sapiens Human MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA pdpk1_human 3-phosphoinositide-dependent protein kinase 1 PDPK1 PDK1 protein protein human Homo sapiens Human MARTTSQLYDAVPIQSSVVLCSCPSPSMVRTQTESSTPPGIPGGSRQGPAMDGTAAEPRPGAGSLQHAQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRLDHPFFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPENILLNEDMHIQITDFGTAKVLSPESKQARANSFVGTAQYVSPELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARDLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTAYLPAMSEDDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQRSGSNIEQYIHDLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ ks6b1_human Ribosomal protein S6 kinase beta-1 RPS6KB1 STK14A Ribosomal protein S6 kinase I p70 ribosomal S6 kinase alpha 70 kDa ribosomal protein S6 kinase 1 Serine/threonine-protein kinase 14A protein protein human Homo sapiens Human MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL ppp2r2b_1a_c 1009309 n/a 1009314 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1009315 unspecified role unspecified role prey prey physical association physical association false false false Fig 3D doxycycline -a semi-synthetic tetracycline antibiotic. Subunit(s) of the catalytic subunit of PP2A were also present in the immunoprecipitation but could not be ascribed to a single gene product. pdpk1_ppp2r2b-1 1009309 n/a 1009316 unspecified role unspecified role bait bait n/a 1009314 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 7B. doxycycline -a semi-synthetic tetracycline antibiotic. s6k-ppp2r2b-pp2ac 1009309 n/a 1009315 unspecified role unspecified role prey prey n/a 1009317 unspecified role unspecified role prey prey n/a 1009314 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig S3B doxycycline -a semi-synthetic tetracycline antibiotic. s6k-2abb-pp2aa 1009309 n/a 1009317 unspecified role unspecified role bait bait n/a 1009314 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig S3A doxycycline -a semi-synthetic tetracycline antibiotic. The catalytic subunit(s) of PP2A were also present in the complex but could not be ascribed to a single gene product. ppp2r2b-pdpk1 1009309 n/a 1009316 unspecified role unspecified role prey prey n/a 1009314 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 7B. doxycycline -a semi-synthetic tetracycline antibiotic. ppp2r2b-pdpk1-1 1009310 n/a 1009316 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1009314 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 7A. doxycycline -a semi-synthetic tetracycline antibiotic. pdpk1-ppp2r2b 1009310 n/a 1009314 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1009316 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 7A. doxycycline -a semi-synthetic tetracycline antibiotic. ppp2r2b-pdk1 1009311 n/a 1009316 unspecified role unspecified role prey prey n/a 1009314 unspecified role unspecified role bait bait physical association physical association false false false Fig 7C. The endogenous interaction between PDK1 and B55-beta was markedly reduced in HEKTERV cells expressing shPPP2R2B. pdpk1-ppp2r2b-1 1009311 n/a 1009314 unspecified role unspecified role prey prey n/a 1009316 unspecified role unspecified role bait bait physical association physical association false false false Fig 7C. The endogenous interaction between PDK1 and B55-beta was markedly reduced in HEKTERV cells expressing shPPP2R2B. pdpk1_ppp2r2b-6 1009312 n/a 1009316 unspecified role unspecified role bait bait n/a 1009314 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 7B. doxycycline -a semi-synthetic tetracycline antibiotic. pdpk1_ppp2r2b-7 1009313 n/a 1009314 unspecified role unspecified role prey prey n/a 1009316 unspecified role unspecified role bait bait physical association physical association false false false Fig 7C. The endogenous interaction between PDK1 and B55-beta was markedly reduced in HEKTERV cells expressing shPPP2R2B.