I2D I2D wimmer-2010-3 SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Wimmer P., Schreiner S., Everett RD., Sirma H., Groitl P., Dobner T. Apoptosis - Interactions involving proteins with a function related to apoptosis Baby rat kidney cells were used as a negative control. Two derivatives of this cell line AB120/AB115, stably expressing E1B-55K or mutant E1B-55K-NES were treated with 20nM leptomycin B for 2 hrs before methanol fixation. thomas.dobner@hpi.uni-hamburg.de Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer wimmer-2010-2 SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML. rat-ab115 Rattus AB115 ab115 ab115-Baby Rat Kidney cell line cell line of polyclonal origin derived from transfection of primary BRK cells with pE1B-NES. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wimmer P., Schreiner S., Everett RD., Sirma H., Groitl P., Dobner T. Apoptosis - Interactions involving proteins with a function related to apoptosis Baby rat kidney cells were used as a negative control. Two derivatives of this cell line AB120/AB115, stably expressing E1B-55K or mutant E1B-55K-NES were treated with 20nM leptomycin B for 2 hrs before methanol fixation. thomas.dobner@hpi.uni-hamburg.de Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer wimmer-2010-4 SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wimmer P., Schreiner S., Everett RD., Sirma H., Groitl P., Dobner T. Apoptosis - Interactions involving proteins with a function related to apoptosis Baby rat kidney cells were used as a negative control. Two derivatives of this cell line AB120/AB115, stably expressing E1B-55K or mutant E1B-55K-NES were treated with 20nM leptomycin B for 2 hrs before methanol fixation. thomas.dobner@hpi.uni-hamburg.de Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer wimmer-2010-8 SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML. rat-ab120 Rat AB120 ab120 AB120 AB120 expresses the Ad5 E1A and wild-type Ad5 E1B-55kDa proteins anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wimmer P., Schreiner S., Everett RD., Sirma H., Groitl P., Dobner T. Apoptosis - Interactions involving proteins with a function related to apoptosis Baby rat kidney cells were used as a negative control. Two derivatives of this cell line AB120/AB115, stably expressing E1B-55K or mutant E1B-55K-NES were treated with 20nM leptomycin B for 2 hrs before methanol fixation. thomas.dobner@hpi.uni-hamburg.de Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer wimmer-2010-1 SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML. rat-ab115 Rattus AB115 ab115 ab115-Baby Rat Kidney cell line cell line of polyclonal origin derived from transfection of primary BRK cells with pE1B-NES. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Wimmer P., Schreiner S., Everett RD., Sirma H., Groitl P., Dobner T. Apoptosis - Interactions involving proteins with a function related to apoptosis Baby rat kidney cells were used as a negative control. Two derivatives of this cell line AB120/AB115, stably expressing E1B-55K or mutant E1B-55K-NES were treated with 20nM leptomycin B for 2 hrs before methanol fixation. thomas.dobner@hpi.uni-hamburg.de Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer wimmer-2010-5 SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML. human-heparg Homo sapiens hepatoma heparg Homo sapiens hepatoma http://www.hpacultures.org.uk/products/celllines/heparg/hepargcells.jsp confocal microscopy confocal microscopy immunostaining immunostaining Wimmer P., Schreiner S., Everett RD., Sirma H., Groitl P., Dobner T. Apoptosis - Interactions involving proteins with a function related to apoptosis Baby rat kidney cells were used as a negative control. Two derivatives of this cell line AB120/AB115, stably expressing E1B-55K or mutant E1B-55K-NES were treated with 20nM leptomycin B for 2 hrs before methanol fixation. thomas.dobner@hpi.uni-hamburg.de Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer wimmer-2010-6 SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML. human-heparg Homo sapiens hepatoma heparg Homo sapiens hepatoma http://www.hpacultures.org.uk/products/celllines/heparg/hepargcells.jsp anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wimmer P., Schreiner S., Everett RD., Sirma H., Groitl P., Dobner T. Apoptosis - Interactions involving proteins with a function related to apoptosis Baby rat kidney cells were used as a negative control. Two derivatives of this cell line AB120/AB115, stably expressing E1B-55K or mutant E1B-55K-NES were treated with 20nM leptomycin B for 2 hrs before methanol fixation. thomas.dobner@hpi.uni-hamburg.de Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer wimmer-2010-7 SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML. rat-ab120 Rat AB120 ab120 AB120 AB120 expresses the Ad5 E1A and wild-type Ad5 E1B-55kDa proteins fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Wimmer P., Schreiner S., Everett RD., Sirma H., Groitl P., Dobner T. Apoptosis - Interactions involving proteins with a function related to apoptosis Baby rat kidney cells were used as a negative control. Two derivatives of this cell line AB120/AB115, stably expressing E1B-55K or mutant E1B-55K-NES were treated with 20nM leptomycin B for 2 hrs before methanol fixation. thomas.dobner@hpi.uni-hamburg.de Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer p29590-5 Protein PML PML MYL RNF71 TRIM19 RING finger protein 71 Tripartite motif-containing protein 19 PML-IV PML-X TRIM19zeta Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNESGFSWGYPHPFLI p03243-1 E1B 55 kDa protein E1B protein, large T-antigen E1B-495R E1B-55K protein protein ade05 Human adenovirus C serotype 5 (HAdV-5) HAdV-5 Human adenovirus 5 MERRNPSERGVPAGFSGHASVESGCETQESPATVVFRPPGDNTDGGAAAAAGGSQAAAAGAEPMEPESRPGPSGMNVVQVAELYPELRRILTITEDGQGLKGVKRERGACEATEEARNLAFSLMTRHRPECITFQQIKDNCANELDLLAQKYSIEQLTTYWLQPGDDFEEAIRVYAKVALRPDCKYKISKLVNIRNCCYISGNGAEVEIDTEDRVAFRCSMINMWPGVLGMDGVVIMNVRFTGPNFSGTVFLANTNLILHGVSFYGFNNTCVEAWTDVRVRGCAFYCCWKGVVCRPKSRASIKKCLFERCTLGILSEGNSRVRHNVASDCGCFMLVKSVAVIKHNMVCGNCEDRASQMLTCSDGNCHLLKTIHVASHSRKAWPVFEHNILTRCSLHLGNRRGVFLPYQCNLSHTKILLEPESMSKVNLNGVFDMTMKIWKVLRYDETRTRCRPCECGGKHIRNQPVMLDVTEELRPDHLVLACTRAEFGSSDEDTD p29590-2 Protein PML PML MYL RNF71 TRIM19 RING finger protein 71 Tripartite motif-containing protein 19 PML-2 PML-V TRIM19beta Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSVSGPEVQPRTPASPHFRSQGAQPQQVTLRLALRLGNFPVRH Ref.5 (AAG50181) sequence is in conflict in position: 578:P->A. Contains a phosphoserine at position 565. p29590-1 Protein PML PML MYL RNF71 TRIM19 RING finger protein 71 Tripartite motif-containing protein 19 PML-I TRIM19alpha Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS p29590-4 Protein PML PML MYL RNF71 TRIM19 RING finger protein 71 Tripartite motif-containing protein 19 PML-3B PML-VI TRIM19epsilon Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAGRERNALW Contains a phosphoserine at position 518. Contains a phosphoserine at position 527. Contains a phosphoserine at position 530. f1m589_rat Pml protein protein rat Rattus norvegicus (Rat) Rat MPPPEEPSEDHEHSQSPAEQAIKEEFQFLRCPGCQTQAKCPKLLPCLHTLCSGCLEAPGLQCPICQAPGSANANAEALDNVFFESLQRRLAVFRQIVDAQAACTRCKELADFWCFECEQLMCGKCFDAHQWYLKHEARPLAELRDNSVREFLDSTRKSNIFCSNTNHRTPAQTNIYCRGCAKPLCCECALLDRNHSHLQCEIGKEIQQLHEELGTMTQALEEQGRTFDSAHAQMHSAIGQLDDARSDTEKQIRARVRQVIEHVQAQERELLEAVNVRYQRDYQEIAGQLGHLEAVLQRIRTGGALVKRMKLYASEQEVLDMHGFLRKALCRLRQEEPQNHKVQLRTSGFEEFKLCLQDLVSFITQRTDVAVAGRASPEAASTHPEAASAQPEAASTHPEAASAQPEAASAHPESASTPGNSTDLEQPKEVQRAQAQAMELSKTQPVAMVKSVPGAHPVPVYAFSMQGPTYREEASQTVSPMKRKCCHIDCCRKIIKMESTDENEDRLSTSSPEQPRPSTSKAISPPHPDGTSSTESTIPEKETPLPNNNHVASDAGETEERIVVISSSEDSDAENLPSHELDDSSSESSSIQLEGPNSLKALDESLAEPHLEDRTLVFFDLKIDNETQKISQLAAVNRESKFRVLIQPEAFSVYSKAVSLESGLQHFLSFLNSMHRPLLACPRLWGPGLPIFFQALNDINKLWEFQDAISGFLAVLPLIQERIPSASSFKLRNLAKTYLARNMSERSALAAVLAMRDLCCLLEVSPGLQLAQHIYSFSSLQCFASLQPLIQAQVLPQPEARLLALHNVSFLELLTAYRSNRQEGLKKYVHYLSLQTTPSSASTQIAQFLQALSAYMDGQLEGHIPAGAEGTALSLSGRAENKGCLA pml_human Protein PML PML MYL RNF71 TRIM19 Tripartite motif-containing protein 19 RING finger protein 71 Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS pml-e1b-55k-3 995659 n/a 995667 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 995668 unspecified role unspecified role bait bait P03243-1:p.Val103Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P03243-1:p.Lys104Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2a, 3a, 5a Binding of E1B-55K is abolished upon superinfection of H1299 cells with 10f.f.u/cell of wild-type virus H5pg4100. pml-e1b-55k-4 995659 n/a 995669 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 995668 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2a pml-e1b-55k-5 995659 n/a 995670 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 995668 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3a Specific binding of E1B-55K is observed upon superinfection of H1299 cells with 10f.f.u/cell of wild-type virus H5pg4100. pml-e1b-55k-6 995659 n/a 995671 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 995668 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3a Specific binding of E1B-55K is observed upon superinfection of H1299 cells with 10f.f.u/cell of wild-type virus H5pg4100. pml-e1b-55k-1 995660 n/a 995668 unspecified role unspecified role prey prey P03243-1:p.[Leu83Ala;Leu87Ala;Leu91Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 995672 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1c pml-e1b-55k-2 995660 n/a 995668 unspecified role unspecified role bait bait P03243-1:p.[Leu83Ala;Leu87Ala;Leu91Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 995672 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1d pml-e1b-55k-7 995661 n/a 995667 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 995668 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2b pml-e1b-55k-8 995661 n/a 995669 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 995668 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2b pml-e1b-55k-1 995662 n/a 995668 unspecified role unspecified role prey prey n/a 995672 unspecified role unspecified role bait bait physical association physical association false false false Fig. 1c pml-e1b-55k-2 995662 n/a 995668 unspecified role unspecified role bait bait n/a 995672 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1d pml-e1b-55k 995663 n/a 995668 unspecified role unspecified role neutral component neutral component P03243-1:p.[Leu83Ala;Leu87Ala;Leu91Ala] leu83ala-leu87ala-leu91ala mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 995672 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 1a,b Leptomycin B E1B-55K-wt accumulates in several bright stained nuclear aggregates clearly colocalizing with endogenous PML. pml-e1b-55k-9 995664 n/a 995668 unspecified role unspecified role neutral component neutral component P03243-1:p.Lys104Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false n/a 995673 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4a pml-e1b-55k-9-10 995665 n/a 995668 unspecified role unspecified role prey prey n/a 995673 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4b pml-e1b-55k 995666 n/a 995668 unspecified role unspecified role neutral component neutral component n/a 995672 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 1a,b Leptomycin B E1B-55K-wt accumulates in several bright stained nuclear aggregates clearly colocalizing with endogenous PML.