I2D I2D shang-2010-6 Dual-specificity phosphatase 26 is a novel p53 phosphatase and inhibits p53 tumor suppressor functions in human neuroblastoma. in vitro In vitro phosphatase assay phosphatase assay tag fluorescence tag visualisation by fluorescence tag fluorescence Shang X., Vasudevan SA., Yu Y., Ge N., Ludwig AD., Wesson CL., Wang K., Burlingame SM., Zhao YJ., Rao PH., Lu X., Russell HV., Okcu MF., Hicks MJ., Shohet JM., Donehower LA., Nuchtern JG., Yang J. Apoptosis - Interactions involving proteins with a function related to apoptosis Phosphatase activity was measured in pmols/min by absorbance at 600nm of free phosphate in a malachite green/molybdate-based assay Confirmed by author: all p53 constructs are of human origin. jianhuay@bcm.edu Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer shang-2010-3 Dual-specificity phosphatase 26 is a novel p53 phosphatase and inhibits p53 tumor suppressor functions in human neuroblastoma. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Shang X., Vasudevan SA., Yu Y., Ge N., Ludwig AD., Wesson CL., Wang K., Burlingame SM., Zhao YJ., Rao PH., Lu X., Russell HV., Okcu MF., Hicks MJ., Shohet JM., Donehower LA., Nuchtern JG., Yang J. Apoptosis - Interactions involving proteins with a function related to apoptosis Confirmed by author: all p53 constructs are of human origin. jianhuay@bcm.edu Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer shang-2010-1 Dual-specificity phosphatase 26 is a novel p53 phosphatase and inhibits p53 tumor suppressor functions in human neuroblastoma. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shang X., Vasudevan SA., Yu Y., Ge N., Ludwig AD., Wesson CL., Wang K., Burlingame SM., Zhao YJ., Rao PH., Lu X., Russell HV., Okcu MF., Hicks MJ., Shohet JM., Donehower LA., Nuchtern JG., Yang J. Apoptosis - Interactions involving proteins with a function related to apoptosis Confirmed by author: all p53 constructs are of human origin. jianhuay@bcm.edu Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer shang-2010-4 Dual-specificity phosphatase 26 is a novel p53 phosphatase and inhibits p53 tumor suppressor functions in human neuroblastoma. human-imr_32 Homo sapiens:Neuroblastoma cell line imr_32 Human IMR-32 Neuroblastoma Human neuroblastoma cell line established from an abdominal mass (diagnosed as neuroblastoma) of a 1-year-old Caucasian boy in 1967. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shang X., Vasudevan SA., Yu Y., Ge N., Ludwig AD., Wesson CL., Wang K., Burlingame SM., Zhao YJ., Rao PH., Lu X., Russell HV., Okcu MF., Hicks MJ., Shohet JM., Donehower LA., Nuchtern JG., Yang J. Apoptosis - Interactions involving proteins with a function related to apoptosis Confirmed by author: all p53 constructs are of human origin. jianhuay@bcm.edu Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer shang-2010-5 Dual-specificity phosphatase 26 is a novel p53 phosphatase and inhibits p53 tumor suppressor functions in human neuroblastoma. human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shang X., Vasudevan SA., Yu Y., Ge N., Ludwig AD., Wesson CL., Wang K., Burlingame SM., Zhao YJ., Rao PH., Lu X., Russell HV., Okcu MF., Hicks MJ., Shohet JM., Donehower LA., Nuchtern JG., Yang J. Apoptosis - Interactions involving proteins with a function related to apoptosis Confirmed by author: all p53 constructs are of human origin. jianhuay@bcm.edu Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer shang-2010-2 Dual-specificity phosphatase 26 is a novel p53 phosphatase and inhibits p53 tumor suppressor functions in human neuroblastoma. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Shang X., Vasudevan SA., Yu Y., Ge N., Ludwig AD., Wesson CL., Wang K., Burlingame SM., Zhao YJ., Rao PH., Lu X., Russell HV., Okcu MF., Hicks MJ., Shohet JM., Donehower LA., Nuchtern JG., Yang J. Apoptosis - Interactions involving proteins with a function related to apoptosis Confirmed by author: all p53 constructs are of human origin. jianhuay@bcm.edu Oncogene (0950-9232) 2010 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Cancer - Interactions investigated in the context of cancer p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD dus26_human Dual specificity protein phosphatase 26 DUSP26 DUSP24 LDP4 MKP8 NATA1 SKRP3 Low-molecular-mass dual-specificity phosphatase 4 Mitogen-activated protein kinase phosphatase 8 Novel amplified gene in thyroid anaplastic cancer Dual specificity phosphatase SKRP3 protein protein human Homo sapiens Human MCPGNWLWASMTFMARFSRSSSRSPVRTRGTLEEMPTVQHPFLNVFELERLLYTGKTACNHADEVWPGLYLGDQDMANNRRELRRLGITHVLNASHSRWRGTPEAYEGLGIRYLGVEAHDSPAFDMSIHFQTAADFIHRALSQPGGKILVHCAVGVSRSATLVLAYLMLYHHLTLVEAIKKVKDHRGIIPNRGFLRQLLALDRRLRQGLEA dusp26-p53-5 982512 n/a 982518 enzyme target enzyme target substrate substrate bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false ser15 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false n/a 982519 enzyme enzyme prey prey Q9BV47:p.Cys152Ser Alters kinetics of interaction mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) dephosphorylation dephosphorylation reaction dephosphorylation false false false Fig. 4g dusp26-p53-7 982512 n/a 982518 enzyme target enzyme target substrate substrate bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false ser37 Undetermined feature position opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false n/a 982519 enzyme enzyme prey prey Q9BV47:p.Cys152Ser Alters kinetics of interaction mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) dephosphorylation dephosphorylation reaction dephosphorylation false false false Fig. 4g dusp26-p53-6 982512 n/a 982518 enzyme target enzyme target substrate substrate bait bait region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false ser20 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain false n/a 982519 enzyme enzyme prey prey Q9BV47:p.Cys152Ser Alters kinetics of interaction mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) dephosphorylation dephosphorylation reaction dephosphorylation false false false Fig. 4g dusp26-p53-2 982513 n/a 982518 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 982519 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9BV47:p.Cys152Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 4d, S4b DUSP26-CS mutant still binds p53 suggesting that phosphatase activity of DUSP26 not required to bind p53. dusp26-p53-3 982513 n/a 982518 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 982519 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 4e dusp26-p53-1 982514 n/a 982518 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 982519 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4c dusp26-p53-4 982515 n/a 982518 unspecified role unspecified role bait bait n/a 982519 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4f dusp26-p53-9 982516 n/a 982518 unspecified role unspecified role prey prey n/a 982519 unspecified role unspecified role bait bait physical association physical association false false false Supp. Fig. 5b dusp26-p53-8 982517 n/a 982518 unspecified role unspecified role bait bait n/a 982519 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. S4a