IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ filimonenko-2010-1 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-2 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-11 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Some participants were visualised using Tag fluorescence. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-5 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-3 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-4 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-7 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. human-neuron Human neurons neuron Neuron [Neuronal cell; Neural cell anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-8 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. human-cls Homo sapiens fibroblast cell line (GM03318) cls Human fibroblast obtained from patients with Coffin-Lowry Syndrome (CLS). GM03318 (NIGMS Human Genetic Cell Repository) Catalog ID GM03318 (NIGMS Human Genetic Cell Repository) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis Fibroblasts were used from a control or Huntingdon's disease patient. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-9 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. rat-neuron Rattus norvegicus neuronal cell neuron Neuron [Neuronal cell; Neural cell anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-10 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. rat-brain-cortex Rattus norvegicus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis A monclonal antibody was used to detect Htt. The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. filimonenko-2010-12 The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Filimonenko M., Isakson P., Finley KD., Anderson M., Jeong H., Melia TJ., Bartlett BJ., Myers KM., Birkeland HC., Lamark T., Krainc D., Brech A., Stenmark H., Simonsen A., Yamamoto A. Apoptosis - Interactions involving proteins with a function related to apoptosis The author confirms that the Alfy and alpha-synuclein constructs used are human in origin, and that the IP shown in Fig S6E was carried out in HeLa cells. Anne.Simonsen@medisin.uio.no,AY46@columbia.edu Anne.Simonsen@medisin.uio.no, AY46@columbia.edu Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. wdfy3_human WD repeat and FYVE domain-containing protein 3 Autophagy-linked FYVE protein WDFY3 KIAA0993 protein protein human Homo sapiens Human MNMVKRIMGRPRQEECSPQDNALGLMHLRRLFTELCHPPRHMTQKEQEEKLYMMLPVFNRVFGNAPPNTMTEKFSDLLQFTTQVSRLMVTEIRRRASNKSTEAASRAIVQFLEINQSEEASRGWMLLTTINLLASSGQKTVDCMTTMSVPSTLVKCLYLFFDLPHVPEAVGGAQNELPLAERRGLLQKVFVQILVKLCSFVSPAEELAQKDDLQLLFSAITSWCPPYNLPWRKSAGEVLMTISRHGLSVNVVKYIHEKECLSTCVQNMQQSDDLSPLEIVEMFAGLSCFLKDSSDVSQTLLDDFRIWQGYNFLCDLLLRLEQAKEAESKDALKDLVNLITSLTTYGVSELKPAGITTGAPFLLPGFAVPQPAGKGHSVRNVQAFAVLQNAFLKAKTSFLAQIILDAITNIYMADNANYFILESQHTLSQFAEKISKLPEVQNKYFEMLEFVVFSLNYIPCKELISVSILLKSSSSYHCSIIAMKTLLKFTRHDYIFKDVFREVGLLEVMVNLLHKYAALLKDPTQALNEQGDSRNNSSVEDQKHLALLVMETLTVLLQGSNTNAGIFREFGGARCAHNIVKYPQCRQHALMTIQQLVLSPNGDDDMGTLLGLMHSAPPTELQLKTDILRALLSVLRESHRSRTVFRKVGGFVYITSLLVAMERSLSCPPKNGWEKVNQNQVFELLHTVFCTLTAAMRYEPANSHFFKTEIQYEKLADAVRFLGCFSDLRKISAMNVFPSNTQPFQRLLEEDVISIESVSPTLRHCSKLFIYLYKVATDSFDSRAEQIPPCLTSESSLPSPWGTPALSRKRHAYHSVSTPPVYPPKNVADLKLHVTTSSLQSSDAVIIHPGAMLAMLDLLASVGSVTQPEHALDLQLAVANILQSLVHTERNQQVMCEAGLHARLLQRCSAALADEDHSLHPPLQRMFERLASQALEPMVLREFLRLASPLNCGAWDKKLLKQYRVHKPSSLSYEPEMRSSMITSLEGLGTDNVFSLHEDNHYRISKSLVKSAEGSTVPLTRVKCLVSMTTPHDIRLHGSSVTPAFVEFDTSLEGFGCLFLPSLAPHNAPTNNTVTTGLIDGAVVSGIGSGERFFPPPSGLSYSSWFCIEHFSSPPNNHPVRLLTVVRRANSSEQHYVCLAIVLSAKDRSLIVSTKEELLQNYVDDFSEESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVKLHYVHSTPGGSGSANPPVVSTVYAYIGTPPAQRQIASLVWRLGPTHFLEEVLPSSNVTTIYELGPNYVGSFQAVCMPCKDAKSEGVVPSPVSLVPEEKVSFGLYALSVSSLTVARIRKVYNKLDSKAIAKQLGISSHENATPVKLIHNSAGHLNGSARTIGAALIGYLGVRTFVPKPVATTLQYVGGAAAILGLVAMASDVEGLYAAVKALVCVVKSNPLASKEMERIKGYQLLAMLLKKKRSLLNSHILHLTFSLVGTVDSGHETSIIPNSTAFQDLLCDFEVWLHAPYELHLSLFEHFIELLTESSEASKNAKLMREFQLIPKLLLTLRDMSLSQPTIAAISNVLSFLLQGFPSSNDLLRFGQFISSTLPTFAVCEKFVVMEINNEEKLDTGTEEEFGGLVSANLILLRNRLLDILLKLIYTSKEKTSINLQACEELVKTLGFDWIMMFMEEHLHSTTVTAAMRILVVLLSNQSILIKFKEGLSGGGWLEQTDSVLTNKIGTVLGFNVGRSAGGRSTVREINRDACHFPGFPVLQSFLPKHTNVPALYFLLMALFLQQPVSELPENLQVSVPVISCRSKQGCQFDLDSIWTFIFGVPASSGTVVSSIHNVCTEAVFLLLGMLRSMLTSPWQSEEEGSWLREYPVTLMQFFRYLYHNVPDLASMWMSPDFLCALAATVFPFNIRPYSEMVTDLDDEVGSPAEEFKAFAADTGMNRSQSEYCNVGTKTYLTNHPAKKFVFDFMRVLIIDNLCLTPASKQTPLIDLLLEASPERSTRTQQKEFQTYILDSVMDHLLAADVLLGEDASLPITSGGSYQVLVNNVFYFTQRVVDKLWQGMFNKESKLLIDFIIQLIAQSKRRSQGLSLDAVYHCLNRTILYQFSRAHKTVPQQVALLDSLRVLTVNRNLILGPGNHDQEFISCLAHCLINLHVGSNVDGFGLEAEARMTTWHIMIPSDIEPDGSYSQDISEGRQLLIKAVNRVWTELIHSKKQVLEELFKVTLPVNERGHVDIATARPLIEEAALKCWQNHLAHEKKCISRGEALAPTTQSKLSRVSSGFGLSKLTGSRRNRKESGLNKHSLSTQEISQWMFTHIAVVRDLVDTQYKEYQERQQNALKYVTEEWCQIECELLRERGLWGPPIGSHLDKWMLEMTEGPCRMRKKMVRNDMFYNHYPYVPETEQETNVASEIPSKQPETPDDIPQKKPARYRRAVSYDSKEYYMRLASGNPAIVQDAIVESSEGEAAQQEPEHGEDTIAKVKGLVKPPLKRSRSAPDGGDEENQEQLQDQIAEGSSIEEEEKTDNATLLRLLEEGEKIQHMYRCARVQGLDTSEGLLLFGKEHFYVIDGFTMTATREIRDIETLPPNMHEPIIPRGARQGPSQLKRTCSIFAYEDIKEVHKRRYLLQPIAVEVFSGDGRNYLLAFQKGIRNKVYQRFLAVVPSLTDSSESVSGQRPNTSVEQGSGLLSTLVGEKSVTQRWERGEISNFQYLMHLNTLAGRSYNDLMQYPVFPWILADYDSEEVDLTNPKTFRNLAKPMGAQTDERLAQYKKRYKDWEDPNGETPAYHYGTHYSSAMIVASYLVRMEPFTQIFLRLQGGHFDLADRMFHSVREAWYSASKHNMADVKELIPEFFYLPEFLFNSNNFDLGCKQNGTKLGDVILPPWAKGDPREFIRVHREALECDYVSAHLHEWIDLIFGYKQQGPAAVEAVNVFHHLFYEGQVDIYNINDPLKETATIGFINNFGQIPKQLFKKPHPPKRVRSRLNGDNAGISVLPGSTSDKIFFHHLDNLRPSLTPVKELKEPVGQIVCTDKGILAVEQNKVLIPPTWNKTFAWGYADLSCRLGTYESDKAMTVYECLSEWGQILCAICPNPKLVITGGTSTVVCVWEMGTSKEKAKTVTLKQALLGHTDTVTCATASLAYHIIVSGSRDRTCIIWDLNKLSFLTQLRGHRAPVSALCINELTGDIVSCAGTYIHVWSINGNPIVSVNTFTGRSQQIICCCMSEMNEWDTQNVIVTGHSDGVVRFWRMEFLQVPETPAPEPAEVLEMQEDCPEAQIGQEAQDEDSSDSEADEQSISQDPKDTPSQPSSTSHRPRAASCRATAAWCTDSGSDDSRRWSDQLSLDEKDGFIFVNYSEGQTRAHLQGPLSHPHPNPIEVRNYSRLKPGYRWERQLVFRSKLTMHTAFDRKDNAHPAEVTALGISKDHSRILVGDSRGRVFSWSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKRLKISSPVRVCQNCYYNLQHERGSEDGPRNC atg5_human Autophagy protein 5 APG5-like Apoptosis-specific protein ATG5 APG5L ASP protein protein human Homo sapiens Human MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD atg12_human Ubiquitin-like protein ATG12 Autophagy-related protein 12 ATG12 APG12 APG12L protein protein human Homo sapiens Human MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG hd_human Huntingtin HTT IT15 HD Huntington disease protein protein protein human Homo sapiens Human MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPKKELSATKKDRVNHCLTICENIVAQSVRNSPEFQKLLGIAMELFLLCSDDAESDVRMVADECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSLRAALWRFAELAHLVRPQKCRPYLVNLLPCLTRTSKRPEESVQETLAAAVPKIMASFGNFANDNEIKVLLKAFIANLKSSSPTIRRTAAGSAVSICQHSRRTQYFYSWLLNVLLGLLVPVEDEHSTLLILGVLLTLRYLVPLLQQQVKDTSLKGSFGVTRKEMEVSPSAEQLVQVYELTLHHTQHQDHNVVTGALELLQQLFRTPPPELLQTLTAVGGIGQLTAAKEESGGRSRSGSIVELIAGGGSSCSPVLSRKQKGKVLLGEEEALEDDSESRSDVSSSALTASVKDEISGELAASSGVSTPGSAGHDIITEQPRSQHTLQADSVDLASCDLTSSATDGDEEDILSHSSSQVSAVPSDPAMDLNDGTQASSPISDSSQTTTEGPDSAVTPSDSSEIVLDGTDNQYLGLQIGQPQDEDEEATGILPDEASEAFRNSSMALQQAHLLKNMSHCRQPSDSSVDKFVLRDEATEPGDQENKPCRIKGDIGQSTDDDSAPLVHCVRLLSASFLLTGGKNVLVPDRDVRVSVKALALSCVGAAVALHPESFFSKLYKVPLDTTEYPEEQYVSDILNYIDHGDPQVRGATAILCGTLICSILSRSRFHVGDWMGTIRTLTGNTFSLADCIPLLRKTLKDESSVTCKLACTAVRNCVMSLCSSSYSELGLQLIIDVLTLRNSSYWLVRTELLETLAEIDFRLVSFLEAKAENLHRGAHHYTGLLKLQERVLNNVVIHLLGDEDPRVRHVAAASLIRLVPKLFYKCDQGQADPVVAVARDQSSVYLKLLMHETQPPSHFSVSTITRIYRGYNLLPSITDVTMENNLSRVIAAVSHELITSTTRALTFGCCEALCLLSTAFPVCIWSLGWHCGVPPLSASDESRKSCTVGMATMILTLLSSAWFPLDLSAHQDALILAGNLLAASAPKSLRSSWASEEEANPAATKQEEVWPALGDRALVPMVEQLFSHLLKVINICAHVLDDVAPGPAIKAALPSLTNPPSLSPIRRKGKEKEPGEQASVPLSPKKGSEASAASRQSDTSGPVTTSKSSSLGSFYHLPSYLKLHDVLKATHANYKVTLDLQNSTEKFGGFLRSALDVLSQILELATLQDIGKCVEEILGYLKSCFSREPMMATVCVQQLLKTLFGTNLASQFDGLSSNPSKSQGRAQRLGSSSVRPGLYHYCFMAPYTHFTQALADASLRNMVQAEQENDTSGWFDVLQKVSTQLKTNLTSVTKNRADKNAIHNHIRLFEPLVIKALKQYTTTTCVQLQKQVLDLLAQLVQLRVNYCLLDSDQVFIGFVLKQFEYIEVGQFRESEAIIPNIFFFLVLLSYERYHSKQIIGIPKIIQLCDGIMASGRKAVTHAIPALQPIVHDLFVLRGTNKADAGKELETQKEVVVSMLLRLIQYHQVLEMFILVLQQCHKENEDKWKRLSRQIADIILPMLAKQQMHIDSHEALGVLNTLFEILAPSSLRPVDMLLRSMFVTPNTMASVSTVQLWISGILAILRVLISQSTEDIVLSRIQELSFSPYLISCTVINRLRDGDSTSTLEEHSEGKQIKNLPEETFSRFLLQLVGILLEDIVTKQLKVEMSEQQHTFYCQELGTLLMCLIHIFKSGMFRRITAAATRLFRSDGCGGSFYTLDSLNLRARSMITTHPALVLLWCQILLLVNHTDYRWWAEVQQTPKRHSLSSTKLLSPQMSGEEEDSDLAAKLGMCNREIVRRGALILFCDYVCQNLHDSEHLTWLIVNHIQDLISLSHEPPVQDFISAVHRNSAASGLFIQAIQSRCENLSTPTMLKKTLQCLEGIHLSQSGAVLTLYVDRLLCTPFRVLARMVDILACRRVEMLLAANLQSSMAQLPMEELNRIQEYLQSSGLAQRHQRLYSLLDRFRLSTMQDSLSPSPPVSSHPLDGDGHVSLETVSPDKDWYVHLVKSQCWTRSDSALLEGAELVNRIPAEDMNAFMMNSEFNLSLLAPCLSLGMSEISGGQKSALFEAAREVTLARVSGTVQQLPAVHHVFQPELPAEPAAYWSKLNDLFGDAALYQSLPTLARALAQYLVVVSKLPSHLHLPPEKEKDIVKFVVATLEALSWHLIHEQIPLSLDLQAGLDCCCLALQLPGLWSVVSSTEFVTHACSLIYCVHFILEAVAVQPGEQLLSPERRTNTPKAISEEEEEVDPNTQNPKYITAACEMVAEMVESLQSVLALGHKRNSGVPAFLTPLLRNIIISLARLPLVNSYTRVPPLVWKLGWSPKPGGDFGTAFPEIPVEFLQEKEVFKEFIYRINTLGWTSRTQFEETWATLLGVLVTQPLVMEQEESPPEEDTERTQINVLAVQAITSLVLSAMTVPVAGNPAVSCLEQQPRNKPLKALDTRFGRKLSIIRGIVEQEIQAMVSKRENIATHHLYQAWDPVPSLSPATTGALISHEKLLLQINPERELGSMSYKLGQVSIHSVWLGNSITPLREEEWDEEEEEEADAPAPSSPPTSPVNSRKHRAGVDIHSCSQFLLELYSRWILPSSSARRTPAILISEVVRSLLVVSDLFTERNQFELMYVTLTELRRVHPSEDEILAQYLVPATCKAAAVLGMDKAVAEPVSRLLESTLRSSHLPSRVGALHGVLYVLECDLLDDTAKQLIPVISDYLLSNLKGIAHCVNIHSQQHVLVMCATAFYLIENYPLDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCALRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKVSPGRTSDPNPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDIMNKVIGEFLSNQQPYPQFMATVVYKVFQTLHSTGQSSMVRDWVMLSLSNFTQRAPVAMATWSLSCFFVSASTSPWVAAILPHVISRMGKLEQVDVNLFCLVATDFYRHQIEEELDRRAFQSVLEVVAAPGSPYHRLLTCLRNVHKVTTC a16l1_human Autophagy-related protein 16-1 APG16-like 1 UNQ9393/PRO34307 ATG16L1 APG16L protein protein human Homo sapiens Human MSSGLRAADFPRWKRHISEQLRRRDRLQRQAFEEIILQYNKLLEKSDLHSVLAQKLQAEKHDVPNRHEISPGHDGTWNDNQLQEMAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQRKDREMQMNEAKIAECLQTISDLETECLDLRTKLCDLERANQTLKDEYDALQITFTALEGKLRKTTEENQELVTRWMAEKAQEANRLNAENEKDSRRRQARLQKELAEAAKEPLPVEQDDDIEVIVDETSDHTEETSPVRAISRAATKRLSQPAGGLLDSITNIFGRRSVSSFPVPQDNVDTHPGSGKEVRVPATALCVFDAHDGEVNAVQFSPGSRLLATGGMDRRVKLWEVFGEKCEFKGSLSGSNAGITSIEFDSAGSYLLAASNDFASRIWTVDDYRLRHTLTGHSGKVLSAKFLLDNARIVSGSHDRTLKLWDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFWDIRSESIVREMELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAIKQTFSAPGFKCGSDWTRVVFSPDGSYVAAGSAEGSLYIWSVLTGKVEKVLSKQHSSSINAVAWSPSGSHVVSVDKGCKAVLWAQY sqstm_human Sequestosome-1 SQSTM1 ORCA OSIL Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 EBI3-associated protein of 60 kDa protein protein human Homo sapiens Human MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL mlp3b_human Microtubule-associated proteins 1A/1B light chain 3B MAP1LC3B MAP1ALC3 Microtubule-associated protein 1 light chain 3 beta MAP1A/MAP1B light chain 3 B MAP1 light chain 3-like protein 2 Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B protein protein human Homo sapiens Human MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV nbr1_human Next to BRCA1 gene 1 protein Neighbor of BRCA1 gene 1 protein Membrane component chromosome 17 surface marker 2 Protein 1A1-3B NBR1 1A13B KIAA0049 M17S2 Cell migration-inducing gene 19 protein MIG19 protein protein human Homo sapiens Human MEPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDLNTIQIKYLDEENEEVSINSQGEYEEALKMAVKQGNQLQMQVHEGHHVVDEAPPPVVGAKRLAARAGKKPLAHYSSLVRVLGSDMKTPEDPAVQSFPLVPCDTDQPQDKPPDWFTSYLETFREQVVNETVEKLEQKLHEKLVLQNPSLGSCPSEVSMPTSEETLFLPENQFSWHIACNNCQRRIVGVRYQCSLCPSYNICEDCEAGPYGHDTNHVLLKLRRPVVGSSEPFCHSKYSTPRLPAALEQVRLQKQVDKNFLKAEKQRLRAEKKQRKAEVKELKKQLKLHRKIHLWNSIHGLQSPKSPLGRPESLLQSNTLMLPLQPCTSVMPMLSAAFVDENLPDGTHLQPGTKFIKHWRMKNTGNVKWSADTKLKFMWGNLTLASTEKKDVLVPCLKAGHVGVVSVEFIAPALEGTYTSHWRLSHKGQQFGPRVWCSIIVDPFPSEESPDNIEKGMISSSKTDDLTCQQEETFLLAKEERQLGEVTEQTEGTAACIPQKAKNVASERELYIPSVDLLTAQDLLSFELLDINIVQELERVPHNTPVDVTPCMSPLPHDSPLIEKPGLGQIEEENEGAGFKALPDSMVSVKRKAENIASVEEAEEDLSGTQFVCETVIRSLTLDAAPDHNPPCRQKSLQMTFALPEGPLGNEKEEIIHIAEEEAVMEEEEDEEDEEEEDELKDEVQSQSSASSEDYIIILPECFDTSRPLGDSMYSSALSQPGLERGAEGKPGVEAGQEPAEAGERLPGGENQPQEHSISDILTTSQTLETVPLIPEVVELPPSLPRSSPCVHHHGSPGVDLPVTIPEVSSVPDQIRGEPRGSSGLVNSRQKSYDHSRHHHGSSIAGGLVKGALSVAASAYKALFAGPPVTAQPIISEDQTAALMAHLFEMGFCDRQLNLRLLKKHNYNILQVVTELLQLNNNDWYSQRY syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA atx1_human Ataxin-1 ATXN1 ATX1 SCA1 Spinocerebellar ataxia type 1 protein protein protein human Homo sapiens Human MKSNQERSNECLPPKKREIPATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHGGGRHGPAGTSVELGLQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAYATPQPGTPVSPVQYAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQQQQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVHLHPHQTMIPHTLTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQAKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSDYSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQQAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAPAIVTSSPQFAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAAPPTLPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEDSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKHSKADGLAGSRHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAPFLTKIEPSKPAATRKRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK mlp3b_rat Microtubule-associated proteins 1A/1B light chain 3B Map1lc3b Map1alc3 Map1lc3 Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B MAP1 light chain 3-like protein 2 MAP1A/MAP1B light chain 3 B Microtubule-associated protein 1 light chain 3 beta protein protein rat Rattus norvegicus (Rat) Rat MPSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESERDEDGFLYMVYASQETFGTALAVTYMSALKATATGREPCL atg5_mouse Autophagy protein 5 Atg5 Apg5l APG5-like protein protein mouse Mus musculus Mouse MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDVSEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAVEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPPEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLREVCPSAVAPEDGEKRSQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIVPQPTD wdfy3_mouse WD repeat and FYVE domain-containing protein 3 Wdfy3 Beach domain, WD repeat and FYVE domain-containing protein 1 protein protein mouse Mus musculus Mouse MNMVKRIMGRPRQEECSPQDNALGLMHLRRLFTELCHPPRHMTQKEQEEKLYMMLPVFNRVFGNAPPNTMTEKFSDLLQFTTQVSRLMVTEIRRRASNKSTEAASRAIVQFLEINQSEEASRGWMLLTTINLLASSGQKTVDCMTTMSVPSTLVKCLYLFFDLPHVPEAGGGAQNELPLAERRGLLQKAFVQILVKLCSFVSPAEELAQKDDLQLLFSAITSWCPPYNLPWRKSAGEVLMTISRHGLSVNVVKYIHEKECLSTCVQNMQQSDDLSPLEIVEMFAGLSCFLKDSSDVSQTLLDDFRIWQGYNFLCDLLLRLEQGKEAECRDALKDLVSLVTSLTTYGVSELKPAGVTTGAPFLLPGFAVPQPAGKGHSVRNIQAFAVLQNAFLKAKTNFLAQIILDAITNIYMADNANYFILESQHTLSQFAEKISKLPEVQNKYFEMLEFVVFSLNYIPCKELISVSILLKSSSSYHCSIIAMKTLLKFTRHDYIFKDVFREVGLLEVMVNLLHKYAALLKDPAQALNEQGDSRNNSSVEDQKHLALLVMEALTVLLQGSNTNAGIFREFGGARCAHNIVKYPQCRQHALMTIQQLVLSPNGEDDMGTLLGLMHSAPPTELQLKTDILRALLSVLRESHRSRTVFRKVGGFVYITSLLVAMERSLSSPPKNGWEKVSQSQVLELLHTVFCTLTAALRYEPANSHFFKTEIQYEKLADAVRFLGCFSDLRKISAVNVFPSNTQPFQRLLEEGAVSVDSVSPTLRHCSKLFIYLYKVATDSFDSHAEQIPPCLTSESSLPSPWGTPALSRKRHAFHCVSTPPVYPAKNVTDLKLQVTSSPLQSSDAVIIHPGAMLAMLDLLASVGSVTQPEHALDLQLAVANILQSLVHTERNQQVMCEAGLHARLLQRCGAALADEDHSLHPPLQRMFERLASQALEPMVLREFLRLASPLNCGAWDKKLLKQYRVHKPSSLSFEPEMRSSVITSLEGLGSDNVFSSHEDNHYRISKSLVKSAEGSTVPLTRVKCLVSMTTPHDIRLHGSSVTPAFVEFDTSLEGFGCLFLPSLAPHNAPTNNTVTTGLTDGAVVSGMGSGERFFPPPSGLSYSCWFCIEHFSSPPNNHPVRLLTVVRRANSSEQHYVCLAIVLSAKDRSLIVSTKEELLQNYVDDFSEESSFYEILPCCARFRCGELVVEGQWHHLALLMSRGMLKNSTAALYLDGQLVSTVKLHYVHSTPGGSGSANPPVLSTVYAYVGTPPAQRQIASLVWRLGPTHFLEEVLPPSSVTTIYELGPNYVGSFQAVCVPCKDAKSEGVTPSPVSLVAEEKVSFGLYALSVSSLTVARIRKVYNKLDSKAIAKQLGISSHENATPVKLVHNAAGHLNGPARTIGAALIGYLGVRTFVPKPVATTLQYIGGAAAILGLVAMASDVEGLYAAVKALVCVVKSNPLASKEMERIKGYQLLAMLLKKKRSLLNSHILHLTFSLVGTVDSGHETSIIPNSTAFQDLLCDFEVWLHAPYELHLSLFEHFIELLTESSEASKNAKLMREFQLIPKLLLTLRDMSLSQPTIAAISNVLSFLLQGFPNSNDLLRFGQFISSTLPTFAVCEKFVVMEINNEEKPDPGAEEEFGGLVSANLILLRNRLLDILLKLVYTSKEKTNINLQACEELVRTLGFDWIMMFMEEHLHPTTVTAAMRILVVLLSNQSILIKFKEGLSGGGWLEQTDSVLTNKIGTVLGFNVGRSAGGRSTVREINRDACHFPGFLVLQSFLPKHTNVPALYFLLMALFLQQPVSELPENLQVSVPVTSSRCKQGCQFDLDSIWTFIFGVPASSGTVVSSIHNVCTESAFLLLGMLRSMLNSPWQSEEEGSWLREYPVTLMQFFRYLYHNVPDLASMWLSPDFLCALAATVFPFNIRPYSEMVTDLDDEVGSPAEEFKAFAADTGMNRSQSEYCNVGTKTYLTNHPAKKFVFDFMRVLIIDNLCLTPASKQTPLIDLLLEASPERSTRTQQKEFQTHVLDSVMDHLLAADVLLGEDASLPITSGGSYQVLVNNVFYFTQRVVDKLWQGMFNKESKLLIDFIIQLIAQSKRRSQGLSLDAVYHCLNRTILYQFSRAHKTVPQQVALLDSLRVLTVNRNLILGPGNHDQEFISCLAHCLINLHAGSVEGFGLEAEARMTTWHIMIPSDIEPDGGYSQDISEGRQLLIKAVNRVWTELIHSKKQVLEELFKVSLPVNDRGHVDIALARPLIEEAGLKCWQNHLAHEKKCISRGEALVPTTQSKLSRVSSGFGLSKLTGSRRNRKESGLHKHSPSPQEISQWMFTHIAVVRDLVDTQYKEYQERQQNALKYVTEEWCQIECELLRERGLWGPPIGSHLDKWMLEMTEGPCRMRKKMVRNDMFYNHYPYVPETEQEASVGKPARYRRAISYDSKEYYLRLASGNPAIVQDAIVESSEGEATQQEPEHGEDTIAKVKGLVKPPLKRSRSAPDGGDEETQEQLQDQIAESGSIEEEEKTDNATLLRLLEEGEKIQHMYRCARVQGLDTSEGLLLFGKEHFYVIDGFTMTATREIRDIETLPPNMHEPIIPRGARQGPSQLKRTCSIFAYEDIKEVHKRRYLLQPIAVEVFSGDGRNYLLAFQKGIRNKVYQRFLAVVPSLTDSSESVSGQRPNTSVEQGSGLLSTLVGEKSVTQRWERGEISNFQYLMHLNTLAGRSYNDLMQYPVFPWILSDYDSEEVDLTNPKTFRNLAKPMGAQTDERLAQYKKRYKDWEDPNGETPAYHYGTHYSSAMIVASYLVRMEPFTQIFLRLQGGHFDLADRMFHSVREAWYSASKHNMADVKELIPEFFYLPEFLFNSNNFDLGCKQNGTKLGDVILPPWAKGDPREFIRVHREALECDYVSAHLHEWIDLIFGYKQQGPAAVEAVNVFHHLFYEGQVDIYNINDPLKETATIGFINNFGQIPKQLFKKPHPPKRVRSRLNGDNIGISVPPGATSDKIFFHHLDNLRPSLTPVKELKEPVGQIVCTDKGILAVEQNKVLIPPAWNKTFAWGYADLSCRLGTYESDKAVTVYECLSEWGQILCAVCPNPKLVITGGTSTVVCVWEMGTSKEKAKPLTLKQALLGHTDTVTCATASLAYHIIVSGSRDRTCIIWDLNKLSFLTQLRGHRAPVSALCINELTGDIVSCAGTYIHVWSINGNPIVSVNTFTGRSQQIVCCCMSEMNEWDTQNVIVTGHSDGVVRFWRMEFLQVPETPAPEPVEDLEMQEGCPEAQIGQQAQDDDSSDSETEEPSVSQDPKDTSSQPSSTSHRPRAASCRATATWCTDSGSDDSRRWSDQLSLDEKDGFIFVNYSEGQTRAHLQGPLAHPHPNPIEARSYSRLKPGYRWERQLVFRSKLTMHTAFDRKDNTHPAEVTALGVSKDHSRILVGDSRGRVFSWSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKRLKISSPVRVCQNCYYSLQHERGAEDGPRNC atg12_mouse Ubiquitin-like protein ATG12 Atg12 Apg12 Apg12l Autophagy-related protein 12 protein protein mouse Mus musculus Mouse MSEDSEVVLQLPSAPVGAGGESLPELSPETATPEPPSSAAVSPGTEEPPGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG sqstm_rat Sequestosome-1 Sqstm1 Zip Protein kinase C-zeta-interacting protein Ubiquitin-binding protein p62 protein protein rat Rattus norvegicus (Rat) Rat MASLTVKAYLLGKEEAAREIRRFSFCFSPEPEAEAAAGPGPCERLLSRVAVLFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRREHRPPCAQEARSMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHREHSKLIFPNPFGHLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPSEDPNVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTSAESSSTGTEDKSGTQPSSCSSEVSKPDGAGEGPAQSLTEQMKKIALESVGQPEELMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL d4a7d8_rat Wdfy3 protein protein rat Rattus norvegicus (Rat) Rat MNMVKRIMGRPRQEECSPQDNALGLMHLRRLFTELCHPPRHMTQKEQEEKLYMMLPVFNRVFGNAPPSTMTEKFSDLLQFTTQVSRLMVTEIRRRASNKSTEAASRAIVQFLEINQSEEASRGWMLLTTINLLASSGQKAVDCMTTMSVPSTLVKCLYLFFDLPHVPEAGGGAQSELPLAERRGLLQKAFVQILVKLCSFVSPAEELAQKDDLQLLFSAITSWCPPYNLPWRKSAGEVLMTISRHGLSVNVVKYIHEKECLSTCVQNMQQSDDLSPLEIVEMFAGLSCFLKDSNSLSSRFLDCFLKKKNIRYFCKYFIQLEQAKEAECRDALKDLVSLVTSLTTYGVSELKPAGVTTGAPFLLPGFSVPQPAGKGHSVRNIQAFAVLQNAFLKAKTNFLAQIILDAITDIYMADSANYFILESQHTLSQFAEKISKLPEVQSKYFEMLEFVVFSLNYIPCKELISVSILLKSSSSHHCSIVAMKTLLKFTRHDYIFKDVFREVGLLEVMVNLLHKYAALLKDPAQALGEQGDSRNNSSVEDQKHLALLVMEALTVLLQGSNTNAGIFREFGGARCAHNIVKYPQCRQHALMTIQQLVLSPNGEDDMGTLLGLMHSAPPTELQLKTDILRALLSVLRESHRSRTVFRKVGGFVYITSLLVAMERSLSSPPKNGWEKVNQNQVFELLHTVFCTLTAALRYEPANSHFFKTEIQYEKLADAVRFLGCFSDLRKISAVNVFPSNTSPFQRLLEEGAVPMDSVSPTLRHCSKLFIYLYKVATDSFDSRTEQIPPCLTSESSLPSPWGTPALSRKRHAFHCVSTPPVYPAKSVTDLKLQVTSSPLPSSEAVIIHPGAMLAMLDLLASVGSVTQPEHALDLQLAVANILQSLVHTERNQQVMCEAGLHARLLQRCGAALADEDHSLHPPLQRMFERLASQALEPMVLREFLRLASPLNCGAWDKKLLKQYRVHKPSSLSFEPDMRSSVVTSLEGLGSDSVFSSHEDNHYRISKSLVKSAEGSTVPLTRVKCLVSMTTPHDIRLHGSSVTPAFVEFDTSLEGFGCLFLPSLAPHNAPTNNAVTTGLTDGAVVSGIGSGERFFPPPSGLSYSCWFCIEHFSSPPNNHPVRLLTVVRRANSSEQHYVCLAIVLSAKDRSLIVSTKEELLQNYVDDFSEESSFYEILPCCARFRCGELVVEGQWHHLALLMSRGMLKNSTAALYIDGQLVSTVKLHYVHSTPGGSGSANPPVLSTVYAYIGTPPAQRQMASLVWRLGPTHFLEEVLPPSSVTAIYELGPDYVGSFQAVLVPCKDSKSEGIIPSPVSLVAEEKVSFGLYALSVSSLTVARIRKVYNKLDSKAIAKQLGISSHENATPVKLVHNAAGHLNGPARTIGAALIGYLGVRTFVPKPVATTLQYIGGAAAILGLVAMASDVEGLYAAVKALVCVVKSNPLASKEMERIKGYQLLAMLLKKRRSLLNSHILHLTFSLVGTVDSGHETSIIPNSTAFQDLLCDFEVWLHAPYELHLSLFEHFIELLTESSEASKNAKLMREFQLIPKLLLTLRDMSLSQPTIAAMSNVLSHLLQGFPNSNDLLRFGQFISSTLPTFAVCEKFVVMEIINEGKPDSGAEEEFGGLVSANLILLRNRLLDILLKLVYTSKEKTNINLQACEELVRTLGFDWIMMFMEEHLHPTTVTAAMRILVVLLSNQSILIKFKEGLSGGGWLEQTDSVLTNKIGTVLGFNVGRSAGGRSTVREINRDACHFPGFLVLQSFLPKHTNVPALYFLLMALFLQQPVSELPENLQVSVPVTNSRCKQGCQFDLDSIWTFIFGVPASSGTVVSSIHNVCTESAFLLLGMLRSMLNSPWQSEEEGSWLREYPVTLMQFFRYLYHNVPDLASMWLSPDFLCALAATVFPFNIRPYSEMVTDLDDEVGSPAEEFKAFAADTGMNRSQSEYCNVGTKTYLTNHPAKKFVFDFMRVLIIDNLCLTPASKQTPLIDLLLEASPERSTRTQQKEFQTHILDSVMDHLLAADVLLGEDASLPITSGGSYQVLVNNVFYFTQRVVDKLWQGMFNKESKLLIDFIIQLIAQSKRRSQGLSLDAVYHCLNRTILYQFSRAHKTVPQQVALLDSLRVLTVNRNLILGPGNHDQEFISCLAHCLINLHAGSSVEGFGLEAEARMTTWHIMIPSDIEPDGGYSQDISEGRQLLIKAVNRVWTELIHSKKQVLEEVFKVSLPVNDRGHVDIAVARPLIEEAGLKGWQNHLAHEKKCISRGEALVPTTQSKLSRVSSGFGLSKLTGSRRNRKESGLHKHSPSPQEISQWMFTHIAVVRDLVDTQYKEYQERQQNALKYVTEEWCQIECELLRERGLWGPPIGSHLDKWMLEMTEGPCRMRKKMVRNDMFYNHYPYVPETEQEASVGKPARYRRAISYDSKEYYLRLASGNPTIVQDAIVESSEGEATQQEPEHGEDTIAKVKGLVKPPLKRSRSAPDGGDEETQEQLQDQIAESGSIEEEEKTDNATLLRLLEEGEKIQHMYRCARVQGLDTSEGLLLFGKEHFYVIDGFTMTATREIRDIETLPPNMHEPIIPRGARQGPSQLKRTCSIFAYEDIKEVHKRRYLLQPIAVEVFSGDGRNYLLAFQKGIRNKVYQRFLAVVPSLTDSSESVSGQRPNTSVEQGSGLLSTLVGEKSVTQRWERGEISNFQYLMHLNTLAGRSYNDLMQYPVFPWILADYDSEEVDLTNPKTFRNLAKPMGAQTDERLAQYKKRYKDWEDPNGETPAYHYGTHYSSAMIVASYLVRMEPFTQIFLRLQGGHFDLADRMFHSVREAWYSASKHNMADVKELIPEFFYLPEFLFNSNNFDLGCKQNGTKLGDVILPPWAKGDPREFIRVHREALECDYVSAHLHEWIDLIFGYKQQGPAAVEAVNVFHHLFYEGQVDIYNINDPLKETATIGFINNFGQIPKQLFKKPHPPKRVRSRLNGENAGLSVPPGATSDKXFHHLDNLRPSLTPVKELKEPVGQIVCTDKGILAVEQNKVLIPPAWNKTFAWGYADLSCRLGTYESDKAVAVYECLSEWGQILCAVCPSPKLVITGGTSTVVCVWEMGTSKEKAKPLTLKQALLGHTDTVTCATASIAYHIIVSGSRDRTCIIWDLNKLSFLTQLRGHRAPVSALCINELTGDIVSCAGTYVHVWSINGNPIVSVNTFTGRSQQIVCCCMSEMNEWDTQNVIVTGHSDGVVRFWRMEFLQVPETPAPEPVEDLEMQEGCPEAQIGQQAQDEDSSDSEAEEPSVSQDPKDTPSQPSSTSHRPRAASCRATATWCTDSGSDDSRRWSDQLSLDEKDGFIFVNYSEGQARAHPQGSHSHPNPTEARSYSRLKPGYRWERQLVFRSKLTMHTAFDRKDNTHPAEVTALGVSKDHSRILVGDSRGRVFSWSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKRLKISSPVRVCQNCYYSLQHERGADDGPRNC The sequence has been withdrawn from uniprot. atg5_rat Autophagy protein 5 Atg5 protein protein rat Rattus norvegicus (Rat) Rat MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDVSEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPCKDAVEAHFMSCVKEADALKHKSQVINEMQRKDHKQLWMGLQNDRFDQFWTINRKLMEYPPEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLREVCPSAVAPEDGEKKSQVMIHGIEPLLETPLQWLSEHLSYPDNFLHISIVPQPTD atg5-alfy 994292 n/a 994303 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false td_tomato fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 994304 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 4B. htt103q_atg16_atg12 994292 n/a 994305 unspecified role unspecified role prey prey n/a 994306 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal_htt_q103 Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false n/a 994307 unspecified role unspecified role prey prey association association false false false Fig 4F. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. htt65q 994292 n/a 994303 unspecified role unspecified role prey prey n/a 994308 unspecified role unspecified role prey prey n/a 994304 unspecified role unspecified role prey prey n/a 994309 unspecified role unspecified role prey prey n/a 994306 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal_htt_q65 Exon1 amino acids from Htt with 65 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false association association false false false Fig 5A. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. htt103q- 994292 n/a 994306 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false n/a 994309 unspecified role unspecified role prey prey n/a 994303 unspecified role unspecified role prey prey n/a 994308 unspecified role unspecified role prey prey n/a 994304 unspecified role unspecified role prey prey association association false false false Fig 5A, 5D. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. Alfie knockdown (si-Alfy) diminished the interaction with Atg5, and a diminished amount of LC3 was present in the complex. htt103q-1 994292 n/a 994303 unspecified role unspecified role prey prey n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 994306 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false physical association physical association false false false Fig 5B. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. The image legend states the c-terminal sequence of Alfy is aa 2543-3527, but the protein ends at aa 3526. Both the N- and C-terminal fragments of Alfy can interact with the polyQ protein. htt65q-2 994292 n/a 994310 unspecified role unspecified role prey prey n/a 994306 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal_htt_q65 Exon1 amino acids from Htt with 65 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false physical association physical association false false false Fig 5G. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. htt103q-3 994292 n/a 994310 unspecified role unspecified role prey prey n/a 994306 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false physical association physical association false false false Fig 5G. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. The image legend states the c-terminal sequence of Alfy is aa 2543-3527, but the protein ends at aa 3526. a_syn_ 994292 n/a 994311 unspecified role unspecified role bait bait region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 994304 unspecified role unspecified role prey prey n/a 994303 unspecified role unspecified role prey prey n/a 994309 unspecified role unspecified role prey prey association association false false false Fig S6E. alfy-httq68 994293 n/a 994306 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminal_htt_q68 Exon1 amino acids from Htt with 68 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false n/a 994303 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 2B. The reference given - Bjorkoy et al. 2005, states the construct used contained Htt amino acids 1-171. However this paper states the Htt sequence was taken from Exon 1 only, which is amino acids 1~90 (including the 21 glutamines residues according to P42858) - this would give a total number of ~140 amino acids used from Htt in this construct, rather than 171. atg5-alfy-wt 994293 n/a 994303 unspecified role unspecified role prey prey n/a 994304 unspecified role unspecified role bait bait colocalization colocalization false false false Fig 4A panel 1. htt65q-1 994293 n/a 994310 unspecified role unspecified role prey prey n/a 994308 unspecified role unspecified role prey prey n/a 994303 unspecified role unspecified role prey prey n/a 994306 unspecified role unspecified role bait bait c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal_htt_q65 Exon1 amino acids from Htt with 65 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false colocalization colocalization false false false Fig 5F. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. alfy-ataxin1 994293 n/a 994312 unspecified role unspecified role neutral component neutral component P54253:p.Lys771Thr mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 994303 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig S 2C/D. Ataxin1 (Q82) containing the nuclear localisation signal mutation K772T did not colocalize in the nucleus with Alfy, though some cytoplasmic colocalisation can be seen. alfy-httq68-1 994294 n/a 994303 unspecified role unspecified role neutral component neutral component n/a 994306 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminal_htt_q68 amino acids from Htt with 68 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 994313 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig S1A. atg5-htt 994294 n/a 994304 unspecified role unspecified role neutral component neutral component n/a 994303 unspecified role unspecified role neutral component neutral component n/a 994306 unspecified role unspecified role neutral component neutral component c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false colocalization colocalization false false false Fig S5A. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. td_alfy-afg5 994294 n/a 994304 unspecified role unspecified role neutral component neutral component n/a 994303 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false td_tomato fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig 4A panels 2, 4 and 6. alfy-atg5 994295 n/a 994314 unspecified role unspecified role prey prey n/a 994315 unspecified role unspecified role bait bait physical association physical association false false false Fig 4E. htt72q-2 994295 n/a 994316 unspecified role unspecified role prey prey n/a 994314 unspecified role unspecified role prey prey n/a 994315 unspecified role unspecified role prey prey n/a 994306 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminal_htt_72q Exon1 amino acids from Htt with 72 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false physical association physical association false false false Fig S6B. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. Alfy can IP in the presence of polyQ proteins in ATG5 knockout cells. gst_atg5-mbp_alfy 994296 n/a 994303 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecobb Escherichia coli (strain B / BL21) n/a 994304 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 4C. atg5-alfy-2 994297 n/a 994303 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 994304 unspecified role unspecified role bait bait physical association physical association false false false Fig 4D. htt72q-wdfy3 994298 n/a 994306 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminal_htt_72q Exon1 amino acids from Htt with 72 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false n/a 994304 unspecified role unspecified role prey prey n/a 994308 unspecified role unspecified role prey prey n/a 994309 unspecified role unspecified role prey prey n/a 994303 unspecified role unspecified role prey prey association association false false false Fig 5A. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. htt103q-2 994299 n/a 994306 unspecified role unspecified role prey prey n/a 994308 unspecified role unspecified role prey prey n/a 994309 unspecified role unspecified role prey prey n/a 994303 unspecified role unspecified role bait bait n/a 994304 unspecified role unspecified role prey prey association association false false false Fig 5C. Immunoprecipitation with Alfy revealed that endogenous Htt comes down with Alfy only in HD patient samples. htt72q-1 994300 n/a 994317 unspecified role unspecified role prey prey n/a 994318 unspecified role unspecified role prey prey n/a 994319 unspecified role unspecified role prey prey n/a 994306 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminal_htt_72q Exon1 amino acids from Htt with 72 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false n/a 994313 unspecified role unspecified role prey prey association association false false false Fig 5E. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. Alfie knockdown (sh-Alfy) diminished the interaction with Atg5, and a diminished amount of LC3 was present in the complex. alfy-httq72_or_79 994301 n/a 994303 unspecified role unspecified role neutral component neutral component td_tomato fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 994306 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n_terminal_htt_q72 Exon1 amino acids from Htt with 72 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false colocalization colocalization false false false Fig 6A, S7C. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. The number of glutamines is stated to be 79 on the image, but 72 on the image legend. Expression of Alfy2981-3526 led to a significant reduction in the aggregate load in the neurons. Overexpression of the full-length 400 kDa Alfy was very difficult to achieve, but in the few cells that were successfully transfected, the frequency of inclusions was fewer, as for Alfy2981-3526. htt103q_alfy 994302 n/a 994306 unspecified role unspecified role neutral component neutral component c terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal_htt_103q Exon1 amino acids from Htt with 103 glutamine residues sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false n/a 994303 unspecified role unspecified role neutral component neutral component n terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false td_tomato tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Fig S6A. The Htt sequence is stated to be "exon1", but the exact amino acid numbers are not given. Both the N- and C-terminal fragments of Alfy interact with the polyQ protein:The N-terminal fragment completely colocalized with the Htt aggregate, while the C-terminal fragment was only found to decorate the outside of the inclusion.