UniProt UniProt http://www.uniprot.org chanda-2010-2 Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Nucleic Acids Res. (0305-1048) 2010 mimix curation chanda-2010-3 Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Nucleic Acids Res. (0305-1048) 2010 mimix curation chanda-2010-6 Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Nucleic Acids Res. (0305-1048) 2010 mimix curation chanda-2010-1 Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down western blot western blot Immuno blot Immuno blot Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Nucleic Acids Res. (0305-1048) 2010 mimix curation chanda-2010-4 Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Nucleic Acids Res. (0305-1048) 2010 mimix curation chanda-2010-5 Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). pull down pull down western blot western blot Immuno blot Immuno blot Chanda D., Xie Y.B., Choi H.S. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of NR0B2/SHP as human according to described pEBG-SHP plasmids (pmid 18657049 and 14752053) though the used sequence numbering of the deletion constructs would fit to mouse or rat origin. Origin of HNF4-alpha according pmid 19651776. Origin of FLAG-SIRT1 according numbering of deletion constructs , myc-SIRT1 according pmid 19690166. hsc@chonnam.ac.kr Nucleic Acids Res. (0305-1048) 2010 mimix curation nr0b2_human Nuclear receptor subfamily 0 group B member 2 NR0B2 SHP Orphan nuclear receptor SHP Small heterodimer partner protein protein human Homo sapiens Human MSTSQPGACPCQGAASRPAILYALLSSSLKAVPRPRSRCLCRQHRPVQLCAPHRTCREALDVLAKTVAFLRNLPSFWQLPPQDQRRLLQGCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEPSSSGGSGQLPDRPQPSLAAVQWLQCCLESFWSLELSPKEYACLKGTILFNPDVPGLQAASHIGHLQQEAHWVLCEVLEPWCPAAQGRLTRVLLTASTLKSIPTSLLGDLFFRPIIGDVDIAGLLGDMLLLR sir1_human NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 SIRT1 SIR2L1 SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein human Homo sapiens Human MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS nr0b2_mouse Nuclear receptor subfamily 0 group B member 2 Nr0b2 Shp Orphan nuclear receptor SHP Small heterodimer partner protein protein mouse Mus musculus Mouse MSSGQSGVCPCQGSAGRPTILYALLSPSPRTRPVAPASHSHCLCQQQRPVRLCAPHRTCREALDVLAKTVAFLRNLPSFCHLPHEDQRRLLECCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEASSGTQGAQPSDRPQPSLAAVQWLQRCLESFWSLELGPKEYAYLKGTILFNPDVPGLRASCHIAHLQQEAHWALCEVLEPWYPASQGRLARILLMASTLKNIPGTLLVDLFFRPIMGDVDITELLEDMLLLR sir1_mouse NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 Sirt1 Sir2l1 SIR2alpha SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein mouse Mus musculus Mouse MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS hnf4a_rat Hepatocyte nuclear factor 4-alpha Hnf4a Hnf-4 Hnf4 Nr2a1 Tcf14 Nuclear receptor subfamily 2 group A member 1 Transcription factor 14 Transcription factor HNF-4 protein protein rat Rattus norvegicus (Rat) Rat MRLSKTLADMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKKIANITDVCESMKEQLLVLVEWAKYIPAFCELLLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYACLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSASDAPHAHHPLHPHLMQEHMGTNVIVANTMPSHLSNGQMCEWPRPRGQAATPETPQPSPPSGSGSESYKLLPGAITTIVKPPSAIPQPTITKQEAI nr0b2-sirt1-2 1243147 n/a 1243153 unspecified role unspecified role bait bait n/a 1243154 unspecified role unspecified role prey prey physical association physical association false false false 2B sirt1-nr0b2-1 1243147 n/a 1243153 unspecified role unspecified role prey prey n/a 1243154 unspecified role unspecified role bait bait physical association physical association false false false 2B nr0b2-sirt1-1 1243148 n/a 1243155 unspecified role unspecified role bait bait n/a 1243156 unspecified role unspecified role prey prey physical association physical association false false false 2B sirt1-nr0b2 1243148 n/a 1243155 unspecified role unspecified role prey prey n/a 1243156 unspecified role unspecified role bait bait physical association physical association false false false 2B nr0b2-sirt1-5 1243149 n/a 1243153 unspecified role unspecified role bait bait n/a 1243154 unspecified role unspecified role prey prey physical association physical association false false false 4B hnf4a-sirt1 1243149 n/a 1243157 unspecified role unspecified role bait bait n/a 1243154 unspecified role unspecified role prey prey physical association physical association false false false 4F nr0b2-sirt1 1243150 n/a 1243153 unspecified role unspecified role bait bait n/a 1243154 unspecified role unspecified role prey prey physical association physical association false false false 2A nr0b2-sirt1-3 1243151 n/a 1243154 unspecified role unspecified role neutral component neutral component n/a 1243153 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2C nr0b2-sirt1-4 1243152 n/a 1243153 unspecified role unspecified role bait bait n/a 1243154 unspecified role unspecified role prey prey physical association physical association false false false 3A, B C and D