UniProt UniProt http://www.uniprot.org guo-2010a-8 DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation guo-2010a-3 DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation guo-2010a-5 DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation guo-2010a-6 DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation guo-2010a-1 DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation guo-2010a-2 DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation guo-2010a-4 DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation guo-2010a-7 DYRK1A and DYRK3 promote cell survival through phosphorylation and activation of SIRT1. in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Guo X., Williams J.G., Schug T.T., Li X. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of p53 (deacetylase assay fig. 3d) according pmid 11672522 (seq numbering) lix3@niehs.nih.gov murine testis cDNA library J. Biol. Chem. (0021-9258) 2010 mimix curation dyr1a_mouse Dual specificity tyrosine-phosphorylation-regulated kinase 1A Dyrk1a Dyrk Protein kinase minibrain homolog MP86 Dual specificity YAK1-related kinase protein protein mouse Mus musculus Mouse MHTGGETSACKPSSVRLAPSFSFHAAGLQMAAQMPHSHQYSDRRQPSISDQQVSALPYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWSLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFAAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLAMRQGADREESPMTGVCVQQSPVASS sir1_human NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 SIRT1 SIR2L1 SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein human Homo sapiens Human MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS dyrk3_mouse Dual specificity tyrosine-phosphorylation-regulated kinase 3 Dyrk3 protein protein mouse Mus musculus Mouse MGGAARDRGRKDAALPGAGLPPQQRRLGDGVYDTFMMIDETKGPPYSDTFSNPSEAPVSRRLNITTEPLTRGHTQHFVNGSEMKVEQLFQEFGNRRSNTLQSDGISNSEKSSPASQGKSSESLSAVKCNLSSRPSKVLPLTPEQALKQYKHHLTAYEKLEIVSYPEIYFVGPNAKKRQGVIGGPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGSFGQVARVYDHKLRQYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTFRNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSLDALHKNKIIHCDLKPENILLKHHGRSATKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGCRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLACMIELLGMPPQKLLEQSKRAKYFINSKGLPRYCSVSTQTDGRVVLLGGRSRRGKKRGPPGSKDWATALKGCGDYLFIEFLKRCLQWDPSARLTPAQALRHPWISKSTPKPLTMDKVPGKRVVNPTNAFQGLGSKLPPVVGIASKLKANLMSETSGSIPLCSVLPKLIS sir1_mouse NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 Sirt1 Sir2l1 SIR2alpha SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein mouse Mus musculus Mouse MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS dyr1a_human Dual specificity tyrosine-phosphorylation-regulated kinase 1A Protein kinase minibrain homolog HP86 Dual specificity YAK1-related kinase DYRK1A DYRK MNB MNBH protein protein human Homo sapiens Human MHTGGETSACKPSSVRLAPSFSFHAAGLQMAGQMPHSHQYSDRRQPNISDQQVSALSYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWNLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFTAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS dyrk3_human Dual specificity tyrosine-phosphorylation-regulated kinase 3 Regulatory erythroid kinase DYRK3 protein protein human Homo sapiens Human MGGTARGPGRKDAGPPGAGLPPQQRRLGDGVYDTFMMIDETKCPPCSNVLCNPSEPPPPRRLNMTTEQFTGDHTQHFLDGGEMKVEQLFQEFGNRKSNTIQSDGISDSEKCSPTVSQGKSSDCLNTVKSNSSSKAPKVVPLTPEQALKQYKHHLTAYEKLEIINYPEIYFVGPNAKKRHGVIGGPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGSFGQVARVYDHKLRQYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTFRNHVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSLDALHKNKIIHCDLKPENILLKHHGRSSTKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLACMMELLGMPPPKLLEQSKRAKYFINSKGIPRYCSVTTQADGRVVLVGGRSRRGKKRGPPGSKDWGTALKGCDDYLFIEFLKRCLHWDPSARLTPAQALRHPWISKSVPRPLTTIDKVSGKRVVNPASAFQGLGSKLPPVVGIANKLKANLMSETNGSIPLCSVLPKLIS p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD dyrk1a-sirt1 1234599 n/a 1234607 unspecified role unspecified role neutral component neutral component n/a 1234608 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1C dyrk1a-dyrk3-2 1234599 n/a 1234608 unspecified role unspecified role neutral component neutral component n/a 1234609 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1C dyrk1a-sirt1-1 1234599 n/a 1234607 unspecified role unspecified role neutral component neutral component n/a 1234610 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S6B dyrk1a-dyrk3-3 1234599 n/a 1234610 unspecified role unspecified role neutral component neutral component n/a 1234609 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S6B sirt1-dyrk3-2 1234600 n/a 1234609 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1234610 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1A, 1D, 1F, S6A and S1C sirt1-dyrk1a 1234600 n/a 1234610 unspecified role unspecified role bait bait n/a 1234607 unspecified role unspecified role prey prey physical association physical association false false false 1A, S6A and S1C dyrk3-sirt1 1234600 n/a 1234609 unspecified role unspecified role bait bait n/a 1234610 unspecified role unspecified role prey prey physical association physical association false false false 1E and S1D dyrk1a-dyrk3 1234601 n/a 1234608 unspecified role unspecified role prey prey n/a 1234611 unspecified role unspecified role bait bait physical association physical association false false false S1B dyrk3-sirt1-4 1234601 n/a 1234612 unspecified role unspecified role bait bait n/a 1234608 unspecified role unspecified role prey prey physical association physical association false false false S1B dyrk3-sirt1-3 1234602 n/a 1234610 enzyme target enzyme target substrate substrate neutral component neutral component n/a 1234609 enzyme enzyme neutral component neutral component direct interaction direct interaction false false false 3E dyrk1a-dyrk3-1 1234602 n/a 1234607 enzyme enzyme neutral component neutral component n/a 1234610 enzyme target enzyme target substrate substrate neutral component neutral component direct interaction direct interaction false false false 3E sirt1-dyrk3 1234603 n/a 1234609 unspecified role unspecified role prey prey n/a 1234610 unspecified role unspecified role bait bait physical association physical association false false false no figure sirt1-dyrk3-1 1234604 n/a 1234609 unspecified role unspecified role prey prey n/a 1234610 unspecified role unspecified role bait bait physical association physical association false false false S1A sirt1-dyrk1a-2 1234605 n/a 1234607 unspecified role unspecified role prey prey n/a 1234609 unspecified role unspecified role prey prey n/a 1234610 unspecified role unspecified role bait bait association association false false false 1B sirt1-tp53-8 1234606 n/a 1234613 enzyme target enzyme target substrate substrate neutral component neutral component n/a 1234610 enzyme enzyme neutral component neutral component direct interaction direct interaction false false false 3D