IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rostovtseva-2009-1 Bax activates endophilin B1 oligomerization and lipid membrane vesiculation. in vitro In vitro fcs fluorescence correlation spectroscopy FCS FCS fcs tag fluorescence tag visualisation by fluorescence tag fluorescence Rostovtseva T.K., Boukari H., Antignani A., Shiu B., Banerjee S., Neutzner A., Youle R.J. Apoptosis - Interactions involving proteins with a function related to apoptosis youler@ninds.nih.gov J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation rostovtseva-2009-2 Bax activates endophilin B1 oligomerization and lipid membrane vesiculation. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight identificat confirmation by molecular weight weight identificat Rostovtseva T.K., Boukari H., Antignani A., Shiu B., Banerjee S., Neutzner A., Youle R.J. Apoptosis - Interactions involving proteins with a function related to apoptosis youler@ninds.nih.gov J. Biol. Chem. (0021-9258) 2009 Only protein-protein interactions imex curation q9y371-2 Endophilin-B1 SH3GLB1 KIAA0491 Bax-interacting factor 1 SH3 domain-containing GRB2-like protein B1 CGI-61 protein protein human Homo sapiens Human MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIMKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRNSQLNSARLEGDNIMIWAEEVTKSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSDLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITYLELLN sh3glb1-sh3glb1-4 1246298 n/a 1246300 putative self putative self neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false alexa-488 label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 physical association physical association false false false 1 sh3glb1-sh3glb1 1246298 n/a 1246300 putative self putative self neutral component neutral component purified purified hist tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false alexa-488 label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1246300 putative self putative self neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false alexa-488 label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1 The presence of human bax is required for this polymerization to happen. sh3glb1-sh3glb1-5 1246299 n/a 1246300 putative self putative self neutral component neutral component purified purified sh3 domain-containing region required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false hist tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) n/a 1246300 putative self putative self neutral component neutral component purified purified sh3 domain-containing region required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 2a and 3a The presence of human bax is required for this polymerization to happen.