IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hammell-2009-1 nhl-2 Modulates microRNA activity in Caenorhabditis elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu C. elegans specific cDNA libraries (Clonetech) Cell (0092-8674) 2009 Only protein-protein interactions 4 imex curation hammell-2009-2 nhl-2 Modulates microRNA activity in Caenorhabditis elegans. caeel Caenorhabditis elegans fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu C. elegans specific cDNA libraries (Clonetech) Cell (0092-8674) 2009 Only protein-protein interactions 4 imex curation hammell-2009-3 nhl-2 Modulates microRNA activity in Caenorhabditis elegans. caeel-larva Immature form of the adult worm larva Immature form of an organism anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu C. elegans specific cDNA libraries (Clonetech) Cell (0092-8674) 2009 Only protein-protein interactions 4 imex curation hammell-2009-4 nhl-2 Modulates microRNA activity in Caenorhabditis elegans. caeel-larva Immature form of the adult worm larva Immature form of an organism anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hammell CM., Lubin I., Boag PR., Blackwell TK., Ambros V. Apoptosis - Interactions involving proteins with a function related to apoptosis victor.ambros@umassmed.edu C. elegans specific cDNA libraries (Clonetech) Cell (0092-8674) 2009 Only protein-protein interactions 4 imex curation q19818_caeel nhl-2 F26F4.7 CELE_F26F4.7 protein protein caeel Caenorhabditis elegans MKKAADRPTSAVNGRHESPATSTNNTQNSGTPSLNGQDPLRSTSSSPLFGTEPLPEDDHASCPYCQQDFRKPRVLDCLHSMCEDCIIAQLDGHLQAKSAAIEEKKRATAAMLDCELETPKVKERPTPPGVIRCPICSQESHVGNDVRYVHAMLLDFVRLAEMKEVEVEKMVAHHRKPCTACKSEQEAIAFCDQCSSDLCENCTTAHTVMNLFQGHTVTTFADLRQPGAHPELRQVMCSTHNQHMRYLCAACETAACKQCLEVDHLNHKIIDINELVINAIREDVTSTVDRVEKKYNNSVADMNSLPDKSQQLNEQYEIAKYRVDKHYEELRNSIEEAYQKSVNDLEEARIRQESNIDEMYRKSQVNEARVHDAINFTRRLLTKGNGLELVVSRKKVIQQFGNLSHAIPSHGHQVELEFYTPSKKQIDHFVHQLTGTVIGRVIQPTVKDVGNATAASTDRSERGGSQPGGGRSSSAANPSQQDEWGRPVYSQNPQLGAIGGERKKNTQNGTNGTRGDSNAFGGWPPSSHPPESTTPPPVTGPQTMITGAPTQTAAPIVSQQQLSNLPNAAAMAAAAAGMRPDLVALAANHMDLAALTNGSTNGSQTPLPESIFPAMWNARQGLPAVATPTTTGLSTNASAAYMQQKLAAAQLQALASHRLNPQIMSMNNLRTNQTIQEFLAMNAMGNLSLNPQLTSAAAAALAATAAANSAVTTTASDVGQSQNSANAQRVSDLKVHSVFGTSQQGSTIRELHCPSGFCLSDTDDILIADTNNHRVVVCGPPHPWKIGRPGTDDGQLCFPRKVIALRGEAVRYVVLDKGGDGKTRAQIFEARGEFVKRLNMMALVPRGGIEVSAAAATPNGQLLLVDTAGFVYSIDVDAPRVTFWFDASTQLGEASDVAMFDNLIYITDFKHHCVQVYTSEGKFIRKMGEPSQTPYPIGIDVSKAGEVLVADTHGNHLHVVVFSPEGQHIHSFTHNEFRLSRCVGLRIAKSGHIVTLCKHNHTLFVFKPLILPNGLPVPTPVPHATSLISKFQ cgh1_caeel ATP-dependent RNA helicase cgh-1 C07H6.5 Conserved germline helicase 1 cgh-1 protein protein caeel Caenorhabditis elegans MSGAEQQQIVPANNGDENWKAGLNLPAKDRRFKTADVTDTKGVEFEDFCLGRDLLMGIFEKGWEKPSPIQEASIGVALTGQDILARAKNGTGKTGAYCIPVIEKIQPALKAIQAMVIVPTRELALQTSQICVELSKHIQLKVMVTTGGTDLRDDIMRLNGTVHLVIATPGRILDLMEKGVAKMEHCKTLVLDEADKLLSQDFQGILDRLINFLPKERQVMLYSATFPNTVTSFMQKHMHKPYEINLMEELTLLGVTQYYAFVQEKQKVHCLNTLFRKLQINQSIIFCNSTQRVELLAKKITEIGYSCYYIHSKMAQNHRNRVFHDFRQGNCRNLVCSDLLTRGIDIQAVNVVINFDFPRNAETYLHRIGRSGRFGHLGVAINLITYEDRHTLRRIEQELRTRIEPIPKTVDPKLYVADQQLVDAADETTA o16720_caeel alg-2 CELE_T07D3.7 T07D3.7 protein protein caeel Caenorhabditis elegans MFPLPVHNGPRLGKLSIFEMPGDSLTSSSFMPDGGAETSSSSQLGGSAHGAIGTKPDAGVQFQCPVRPNHGVEGRSILLRANHFAVRIPGGSVQHYQIDVFPDKCPRRVNREVIGCLISSFSKYFTNIRPVYDGKRNMYTREPLPIGTEPMNFEVTLPGDSAVERKFSVTMKWIGQVCLSALDDAMEGRVRQVPHEAVQSIDVILRHLPSLKYTPVGRSFFTPPGVMKPGMQMHQESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCDIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICKLVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGKQFHTGMEVRTWAIACFAQQSHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIITDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDRDHGSGEEGSQPSGTSSEDTTLSSMAKAVQVHPDSNNVMYFA g5egr6_caeel alg-1 CELE_F48F7.1 F48F7.1 protein protein caeel Caenorhabditis elegans MSGGPQYLPGVMNSTIQQQPQSATSSFLPSGPISSTSTSSQVVPTSGATQQPPFPSAQAAASTALQNDLEEIFNSPPTQPQTFSDVPQRQAGSLAPGVPIGNTSVSIGEPANTLGGGLPGGAPGQLPGGNQSGIQFQCPRRPNHGVEGRSILLRANHFAVRIPGGTIQHYQVDVTPDKCPRRVNREIISCLISAFSKYFTNIRPVYDGKRNMYTREPLPIGRERMDFDVTLPGDSAVERQFSVSLKWVGQVSLSTLEDAMEGRVRQVPFEAVQAMDVILRHLPSLKYTPVGRSFFSPPVPNASGVMAGSCPPQASGAVAGGAHSAGQYHAESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRSMPVIEFIAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGQMRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFYDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCNIVPGQRCIKKLTDVQTSTMIKATARSAPEREREISNLVRKAEFSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRTRATALPNQGVWDMRGKQFHTGIDVRVWAIACFAQQQHVKENDLRMFTNQLQRISNDAGMPIVGNPCFCKYAVGVEQVEPMFKYLKQNYSGIQLVVVVLPGKTPVYAEVKRVGDTVLGIATQCVQAKNAIRTTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFFGCDITHPPAGDSRKPSIAAVVGSMDAHPSRYAATVRVQQHRQEIISDLTYMVRELLVQFYRNTRFKPARIVVYRDGVSEGQFFNVLQYELRAIREACMMLERGYQPGITFIAVQKRHHTRLFAVDKKDQVGKAYNIPPGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDREHDSGEGSQPSGTSEDTTLSNMARAVQVHPDANNVMYFA q17740_caeel ain-1 C06G1.4 CELE_C06G1.4 protein protein caeel Caenorhabditis elegans MESLAKNFNNISIDDAAWHPNDQQPVWGNNGLQQQRVWAPPVPQPQFPNRSPWQQESNDIEPNVNEALVGLSGTGSSSQSQWGGIQNTDYEKQIWGDPPLSDVQYPLQPHASFGELNSLGGQSAPWSIGSQPAHPVWSNGGMGKESDDFWKQQQPQQHQQYPMPLLQGAWNTGMRHGPPGQGQRSGPMMQQQQDYQGGWGGPNNMGGNKQMNRPWGQDSNRGMSGMPPRNGRPNHNQGRYSGNMGGGVDVSVPPPMDMHMGGGMGGMRNMGVAPNSGHGSWKNNNAGGGQGGHGGQRNYNNRGGMGHSGSQSGGGSNMWNSTGNNGSGGSMQQFMGGDQQYNNPFPIGGAADDLTLTVWHDPNGELKKWQRDTGVSYWGDPEKQTDRTISLWIVGEGADEDLETALNRCPVPQKKGEDNQRFPFPVPAKRPIVVTGWGELPENDPNNPSKSESTIFDENNRWNDLTTEQNPWYLPNQHTSTFSSDNTTGSWVQGGTIPLNEPGTNQHVAVAEMLKNAVDKGYLDQSVTMMANLPPMVLQYVNMLLVKIPALDSVENELKQIIESSLPEDVKEVDPQNQQKYMNDSQKLEHNRLIIEVTTAKIEVQEYSKKVNRALMDAGIVQPQEQRAPASTEDYHYSFLE nhl2-cgh1-1 Interaction between NHL2 abd CGH1 determined by Y2H 802080 n/a 802084 unspecified role unspecified role bait bait n/a 802085 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4B, 4C nhl2-cgh1-2 Colocalisation of NHL2 abd CGH1 802081 n/a 802084 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 802085 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 4D, 4CE alg1-cgh1-1 Interaction between CGH1, ALG1 and ALG2 determined by coimmunoprecipitation 802082 n/a 802085 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 802086 unspecified role unspecified role prey prey n/a 802087 unspecified role unspecified role prey prey association association false false false Fig. 6B nhl2-cgh1-4 Interaction between CGH1, ALG1 and ALG2 determined by coimmunoprecipitation 802083 n/a 802088 unspecified role unspecified role prey prey n/a 802087 unspecified role unspecified role prey prey n/a 802086 unspecified role unspecified role prey prey n/a 802085 unspecified role unspecified role bait bait n/a 802084 unspecified role unspecified role prey prey association association false false false Fig. 6C, 6D RNase treatment of immunoprecipitated material reduces CGH-1 binding to core miRISC components but does not dramatically affect its interaction with NHL-2. Coimmunoprecipitation of ALG-1/2 and AIN-1 with CGH-1 is not affected in the absence of nhl-2.