IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tang-2008c-2 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation tang-2008c-3 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation tang-2008c-4 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. rat-brain-cortex Rattus norvegicus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation tang-2008c-5 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation tang-2008c-8 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation tang-2008c-1 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation tang-2008c-6 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. rat-brain-cortex Rattus norvegicus brain cortex brain_cortex Brain cortex [Cerebral cortex; Cerebrum cortex; Cortex; Pallium] confocal microscopy confocal microscopy immunostaining immunostaining Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation tang-2008c-7 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain confocal microscopy confocal microscopy immunostaining immunostaining Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation tang-2008c-9 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation tang-2008c-10 Netrin-1 mediates neuronal survival through PIKE-L interaction with the dependence receptor UNC5B. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tang X., Jang S.W., Okada M., Chan C.B., Feng Y., Liu Y., Luo S.W., Hong Y., Rama N., Xiong W.C., Mehlen P., Ye K. Apoptosis - Interactions involving proteins with a function related to apoptosis The origins of the agap2, unc5b and dcc constructs were given by the author upon email request. The GTPase domain location information was inferred from PMID:14528310, by the same group. The source of Fyn could not be traced. kye@emory.edu Human fetal brain cDNA Nat. Cell Biol. (1465-7392) 2008 Only protein-protein interactions imex curation unc5b_human Netrin receptor UNC5B UNC5B P53RDL1 UNC5H2 Protein unc-5 homolog 2 Protein unc-5 homolog B p53-regulated receptor for death and life protein 1 UNQ1883/PRO4326 protein protein human Homo sapiens Human MGARSGARGALLLALLLCWDPRLSQAGTDSGSEVLPDSFPSAPAEPLPYFLQEPQDAYIVKNKPVELRCRAFPATQIYFKCNGEWVSQNDHVTQEGLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKNFDQEPLGKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPTQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTICPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCMQNKKTLSDPNSHLLEASGDAALYAGLVVAIFVVVAILMAVGVVVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSVPPDLTASAGIYRGPVYALQDSTDKIPMTNSPLLDPLPSLKVKVYSSSTTGSGPGLADGADLLGVLPPGTYPSDFARDTHFLHLRSASLGSQQLLGLPRDPGSSVSGTFGCLGGRLSIPGTGVSLLVPNGAIPQGKFYEMYLLINKAESTLPLSEGTQTVLSPSVTCGPTGLLLCRPVILTMPHCAEVSARDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEPRACHILLDQLGTYVFTGESYSRSAVKRLQLAVFAPALCTSLEYSLRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLMFKDSYHNLRLSLHDLPHAHWRSKLLAKYQEIPFYHIWSGSQKALHCTFTLERHSLASTELTCKICVRQVEGEGQIFQLHTTLAETPAGSLDTLCSAPGSTVTTQLGPYAFKIPLSIRQKICNSLDAPNSRGNDWRMLAQKLSMDRYLNYFATKASPTGVILDLWEALQQDDGDLNSLASALEEMGKSEMLVAVATDGDC agap2_rat Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 Agap2 Centg1 Centaurin-gamma-1 Phosphatidylinositol 3-kinase enhancer protein protein rat Rattus norvegicus (Rat) Rat MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAVGAPGARGSEPRDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGAGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAVAAGGGGSAAVTTSGGVGAGAGARGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVTAASTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRASPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKPESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSHLHGQLISLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV dcc_rat Netrin receptor DCC Dcc Tumor suppressor protein DCC protein protein rat Rattus norvegicus (Rat) Rat MENSLGCVWVPKLAFVLFGASLLSAHLQVTGFQIKPFTSLHFVSEPSDAVTMRGGNVLLNCSAESDRGVPVIKWKKDGLILALGMDDRKQQLPNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVMVAGPLRFLSQTESITAFMGDTVLLKCEVIGDPMPTIHWQKNQQDLNPIPGDSRVVVLPSGALQISRLQPGDSGVYRCSARNPASTRTGNEAEVRILSDPGLHRQLYFLQRPSNVIAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGTYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECAVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQSSAQLIVPKPAIPSSSILPSAPRDVVPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLHAVSASPTSILITWEPPAYANGPVQGYRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYTLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVSLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSGPDVSTPMLPPVGVQAVALTHEAVRVSWADNSVPKNQKTSDVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQIMDLSLDTMYYFRIQARNAKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVITVVTVGVLTVLVVVIVAVICTRRSSAQQRKKRATHSASKRKGSQKDLRPPDLWIHHEEMEMKNIEKPAGTDPAGRGSPIQSCQDLTPVSHSQSESQMGSKSASHSGQDTEEAGSSMSTLERSLAARRATRTKLMIPMEAQSNNPAVVSAIPVPTLESAQYPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRTVSEGPTAQQQPMLPPAQPEHPSSEEAPSRTIPTACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVPTAPEVSEESHKPTEDPASVYEQDDLSEQMASLEGLMKQLNAITGSAF unc5b_rat Netrin receptor UNC5B Unc5b Unc5h2 Protein unc-5 homolog 2 Protein unc-5 homolog B protein protein rat Rattus norvegicus (Rat) Rat MRARSGARGALLLALLLCWDPTPSLAGIDSGGQALPDSFPSAPAEQLPHFLLEPEDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQKGHVTQESLDEATGLRIREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQACQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSRPLEPSGDVALYAGLVVAVFVVLAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYDSSTIGSGAGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLTIPGTGVSLLVPNGAIPQGKFYDLYLRINKTESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFTGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPAALKEVLELERTLGGYLVEEPKTLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQKALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAATTQLGPYAFKIPLSIRQKICNSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMTTDGDC agap2_mouse Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 Agap2 Centg1 Centaurin-gamma-1 Kiaa0167 Phosphatidylinositol 3-kinase enhancer protein protein mouse Mus musculus Mouse MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAPGARGSEPRDPGSPRGSEEPGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAAAAGGGGSAAVTTSGGVGAGAGTRGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVTATSTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRTSPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKIESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDAEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV unc5b_mouse Netrin receptor UNC5B Unc5b Unc5h2 Protein unc-5 homolog 2 Protein unc-5 homolog B protein protein mouse Mus musculus Mouse MRARSGVRSALLLALLLCWDPTPSLAGVDSAGQVLPDSYPSAPAEQLPYFLLEPQDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQNDHVTQESLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSHPLETSGDVALYAGLVVAVFVVVAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPNNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYNSSTIGSGSGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLSLPGTGVSLLVPNGAIPQGKFYDLYLHINKAESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFMGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQRALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAITTQLGPYAFKIPLSIRQKICSSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMATDGDC dcc_mouse Netrin receptor DCC Dcc Tumor suppressor protein DCC protein protein mouse Mus musculus Mouse MENSLGCVWVPKLAFVLFGASLLSAHLQVTGFQIKPFTSLHFVSEPSDAVTMRGGNVLLNCSAESDRGVPVIKWKKDGLILALGMDDRKQQLPNGSLLIQNILHSRHHKPDEGLYQCEASLADSGSIISRTAKVTVAGPLRFLSQTESITAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLNPLPGDSRVVVLPSGALQISRLQPGDSGVYRCSARNPASIRTGNEAEVRILSDPGLHRQLYFLQRPSNVIAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGTYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECAVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQSSAQLIVPKPAIPSSSILPSAPRDVLPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLHAVSTSPTSILITWEPPAYANGPVQGYRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYTLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNISLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSGPDVSTPMLPPVGVQAVALTHEAVRVSWADNSVPKNQKTSDVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQIMDLSLDTMYYFRIQARNVKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVITVVTVGVLTVLVVVIVAVICTRRSSAQQRKKRATHSVSKRKGSQKDLRPPDLWIHHEEMEMKNIEKPTGTDPAGRDSPIQSCQDLTPVSHSQSETQMGSKSASHSGQDTEDAGSSMSTLERSLAARRATRAKLMIPMEAQSSNPAVVSAIPVPTLESAQYPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRTQSVSEGPTTQQQPMLPPAQPEHPSSEEAPSRTIPTACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVPTAPEVSEESHKPTEDPASVYEQDDLSEQMASLEGLMKQLNAITGSAF agap2-unc5b-2 1186291 n/a 1186301 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false c-terminal region without death domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false n/a 1186302 unspecified role unspecified role bait bait gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1b unc5b-agap2 1186291 n/a 1186302 unspecified role unspecified role prey prey gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186301 unspecified role unspecified role bait bait c-terminal region sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal region without death domain required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1b agap2-unc5b-3 1186291 n/a 1186302 unspecified role unspecified role bait bait myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false gtp-ase domain required to bind necessary binding region required to bind mutation analysis mutation analysis range range range range false n/a 1186301 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1b unc5b-agap2-4 1186291 n/a 1186302 unspecified role unspecified role prey prey Q8CGU4:p.[Lys413Ala;Ser414Asn] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186301 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2a The presence of purified netrin-1 is required for the interaction to happen. unc5b-agap2-5 1186291 n/a 1186301 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186302 unspecified role unspecified role prey prey Q8CGU4:p.[Tyr1032Phe;Tyr1124Phe] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8CGU4:p.Tyr1124Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8CGU4:p.Tyr1032Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2b, 3a The presence of purified netrin-1 is required for the interaction to happen. The interaction is inhibited by PP2 (selective inhibitor of Fyn, a tyrosine kinase that phosphorylates unc5b), genistein and compund 5 (both tyrosine kinase inhibitors). agap2-unc5b-9 1186291 n/a 1186302 unspecified role unspecified role bait bait Q8CGU4:p.Tyr1124Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q8CGU4:p.Tyr1032Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q8CGU4:p.[Tyr1032Phe;Tyr1124Phe] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1186301 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2b, S6, 3a, S1c The presence of purified netrin-1 is required for the interaction to happen (figs. 2b, S6 and 3a). In the absence of netrin-1, the presence of FynA is required (fig. S1c). The interaction is inhibited by PP2 (selective inhibitor of Fyn, a tyrosine kinase that phosphorylates unc5b), genistein and compund 5 (both tyrosine kinase inhibitors). dcc-agap2-1 1186291 n/a 1186302 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186303 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false S2b unc5b-agap2-1 1186292 n/a 1186301 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186302 unspecified role unspecified role prey prey gtp-ase domain required to bind necessary binding region required to bind mutation analysis mutation analysis range range range range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1b unc5b-agap2-7 1186292 n/a 1186301 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186302 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false S1c The presence of FynA is required for the interaction to happen. agap2-unc5b-4 1186293 n/a 1186302 unspecified role unspecified role bait bait n/a 1186304 unspecified role unspecified role prey prey physical association physical association false false false 1c, S6, 2c This interaction is disrupted by tyrosine-kinase inhibitors genistein adn PP2 (fig. 2c). The presence of netrin-1 increases the interaction (fig. 2c). unc5b-agap2-6 1186293 n/a 1186304 unspecified role unspecified role bait bait n/a 1186302 unspecified role unspecified role prey prey physical association physical association false false false 1c, S6, 2c This interaction is disrupted by tyrosine-kinase inhibitors genistein adn PP2 (fig. 2c). The presence of netrin-1 increases the interaction (fig. 2c). agap2-unc5b-5 1186294 n/a 1186304 unspecified role unspecified role prey prey n/a 1186302 unspecified role unspecified role bait bait physical association physical association false false false 1c, S6 unc5b-agap2-3 1186294 n/a 1186304 unspecified role unspecified role bait bait n/a 1186302 unspecified role unspecified role prey prey physical association physical association false false false 1c, S6 agap2-unc5b-12 1186295 n/a 1186305 unspecified role unspecified role unspecified role unspecified role n/a 1186306 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false 3b The presence of FynA is required for the interaction to happen. dcc-agap2-2 1186295 n/a 1186305 unspecified role unspecified role prey prey n/a 1186307 unspecified role unspecified role bait bait physical association physical association false false false S2c agap2-unc5b 1186296 n/a 1186301 unspecified role unspecified role prey prey c-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1186302 unspecified role unspecified role bait bait gtp-ase domain sufficient to bind sufficient binding region sufficient to bind range range range range false 1186388 1186386 physical association physical association false false false 1a agap2-unc5b-7 1186297 n/a 1186302 unspecified role unspecified role neutral component neutral component n/a 1186304 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1d agap2-unc5b-8 1186298 n/a 1186304 unspecified role unspecified role neutral component neutral component n/a 1186302 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1d dcc-agap2 1186299 n/a 1186303 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186302 unspecified role unspecified role prey prey cell lysate cell lysate myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false S2a unc5b-dcc 1186300 n/a 1186301 unspecified role unspecified role bait bait ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1186303 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false S2d The presence of either netrin-1, Fyn or GFP-agap2 is required for the interaction to happen.