IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ma-2008-1 SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 Only protein-protein interactions 17 imex curation ma-2008-2 SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 Only protein-protein interactions 17 imex curation ma-2008-5 SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 Only protein-protein interactions 17 imex curation ma-2008-6 SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 Only protein-protein interactions 17 imex curation ma-2008-3 SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 Only protein-protein interactions 17 imex curation ma-2008-4 SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Ma XM., Yoon SO., Richardson CJ., Julich K., Blenis J. Apoptosis - Interactions involving proteins with a function related to apoptosis Author used 293E cells - a sub-clone of 293 cells specifically expressing the E1a gene from adenovirus type 5. Source of transfected NCBP1 supplied by author, and identified by anti-tag western jblenis@hms.harvard.edu Insulin (mM) Rapamycin (ng/ml) Cell (0092-8674) 2008 Only protein-protein interactions 17 imex curation ncbp1_human Nuclear cap-binding protein subunit 1 NCBP1 CBP80 NCBP 80 kDa nuclear cap-binding protein protein protein human Homo sapiens Human MSRRRHSDENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYHIVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMNTTCVDRFINWFSHHLSNFQFRWSWEDWSDCLSQDPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPANPTCIYKYGDESSNSLPGHSVALCLAVAFKSKATNDEIFSILKDVPNPNQDDDDDEGFSFNPLKIEVFVQTLLHLAAKSFSHSFSALAKFHEVFKTLAESDEGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSRDFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRSSDRKDGVLEEQIERLQEKVESAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSVLTPWYKNCIERLQQIFLQHHQIIQQYMVTLENLLFTAELDPHILAVFQQFCALQA ks6b1_human Ribosomal protein S6 kinase beta-1 RPS6KB1 STK14A Ribosomal protein S6 kinase I p70 ribosomal S6 kinase alpha 70 kDa ribosomal protein S6 kinase 1 Serine/threonine-protein kinase 14A protein protein human Homo sapiens Human MRRRRRRDGFYPAPDFRDREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLESVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL eif3b_human Eukaryotic translation initiation factor 3 subunit B Eukaryotic translation initiation factor 3 subunit 9 eIF-3-eta eIF3 p116 eIF3 p110 Prt1 homolog EIF3B EIF3S9 protein protein human Homo sapiens Human MQDAENVAVPEAAEERAEPGQQQPAAEPPPAEGLLRPAGPGAPEAAGTEASSEEVGIAEAGPESEVRTEPAAEAEAASGPSESPSPPAAEELPGSHAEPPVPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEPRALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLATFHQRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGHKKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIPARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKNNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMAQELYMEQKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEIIPLGNQE if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV mtor_human Serine/threonine-protein kinase mTOR MTOR FRAP FRAP2 FK506-binding protein 12-rapamycin complex-associated protein 1 Rapamycin target protein 1 FRAP1 Mammalian target of rapamycin FKBP12-rapamycin complex-associated protein Mechanistic target of rapamycin RAFT1 RAPT1 Rapamycin and FKBP12 target 1 protein protein human Homo sapiens Human MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLIKQATSHENLCQCYIGWCPFW ren3b_human Regulator of nonsense transcripts 3B RENT3B Nonsense mRNA reducing factor 3B Up-frameshift suppressor 3 homolog B UPF3X UPF3B Up-frameshift suppressor 3 homolog on chromosome X protein protein human Homo sapiens Human MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQKNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE pdip3_human Polymerase delta-interacting protein 3 POLDIP3 KIAA1649 PDIP46 46 kDa DNA polymerase delta interaction protein S6K1 Aly/REF-like target protein protein human Homo sapiens Human MADISLDELIRKRGAAAKGRLNARPGVGGVRSRVGIQQGLLSQSTRTATFQQRFDARQKIGLSDARLKLGVKDAREKLLQKDARFRIKGKVQDAREMLNSRKQQTTVPQKPRQVADAREKISLKRSSPAAFINPPIGTVTPALKLTKTIQVPQQKAMAPLHPHPAGMRINVVNNHQAKQNLYDLDEDDDGIASVPTKQMKFAASGGFLHHMAGLSSSKLSMSKALPLTKVVQNDAYTAPALPSSIRTKALTNMSRTLVNKEEPPKELPAAEPVLSPLEGTKMTVNNLHPRVTEEDIVELFCVCGALKRARLVHPGVAEVVFVKKDDAITAYKKYNNRCLDGQPMKCNLHMNGNVITSDQPILLRLSDSPSMKKESELPRRVNSASSSNPPAEVDPDTILKALFKSSGASVTTQPTEFKIKL rs6_human 40S ribosomal protein S6 RPS6 Phosphoprotein NP33 Small ribosomal subunit protein eS6 OK/SW-cl.2 protein protein human Homo sapiens Human MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK if4a3_human Eukaryotic initiation factor 4A-III KIAA0111 EIF4A3 DDX48 Eukaryotic translation initiation factor 4A isoform 3 ATP-dependent RNA helicase eIF4A-3 ATP-dependent RNA helicase DDX48 DEAD box protein 48 Eukaryotic initiation factor 4A-like NUK-34 Nuclear matrix protein 265 protein protein human Homo sapiens Human MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI srsf2_human Serine/arginine-rich splicing factor 2 Splicing factor SC35 Splicing component, 35 kDa Protein PR264 SRSF2 SFRS2 Splicing factor, arginine/serine-rich 2 protein protein human Homo sapiens Human MSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRSPRRRRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPVSKRESKSRSRSKSPPKSPEEEGAVSS eif3b-eif4e-1 Interaction between EIF3B, EIF4E and RPS6KB1 by coimmunoprecipitation 738551 N/A 738557 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 738558 unspecified role unspecified role prey prey N/A 738559 unspecified role unspecified role bait bait N/A 738560 unspecified role unspecified role prey prey association association false false false Fig. 1B 100 20 Q09161 transfection eif3b-eif4e-6 Interaction between EIF3B, EIF4E and RPS6KB1 by coimmunoprecipitation 738551 n/a 738559 unspecified role unspecified role bait bait n/a 738557 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 738560 unspecified role unspecified role prey prey n/a 738558 unspecified role unspecified role prey prey association association false false false Fig. 1B 0 0 Q09161 transfection eif3b-eif4e-2 Interaction between EIF3B, EIF4E and FRAP1 by coimmunoprecipitation 738551 N/A 738560 unspecified role unspecified role prey prey N/A 738561 unspecified role unspecified role prey prey N/A 738559 unspecified role unspecified role bait bait N/A 738557 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 1B 100 0 Q09161 transfection eif4e-eif3b Interaction between EIF3B, EIF4E, RPS6 and FRAP1 by coimmunoprecipitation 738551 N/A 738559 unspecified role unspecified role prey prey N/A 738560 unspecified role unspecified role bait bait N/A 738558 unspecified role unspecified role prey prey region nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 738561 unspecified role unspecified role prey prey association association false false false Fig. 1B 100 0 RPS6KB1 is weakly serine phosphorylated eif4e-eif3b-1 Interaction between EIF3B, EIF4E and RPS6KB1 by coimmunoprecipitation 738551 N/A 738558 unspecified role unspecified role prey prey N/A 738559 unspecified role unspecified role prey prey N/A 738560 unspecified role unspecified role bait bait N/A 738561 unspecified role unspecified role prey prey association association false false false Fig. 1B 100 20 eif4e-eif3b-2 Interaction between EIF3B, EIF4E and RPS6KB1 by coimmunoprecipitation 738551 n/a 738560 unspecified role unspecified role bait bait n/a 738561 unspecified role unspecified role prey prey n/a 738559 unspecified role unspecified role prey prey n/a 738558 unspecified role unspecified role prey prey association association false false false Fig. 1B 0 0 ncbp1-poldip3 Interaction between NCBP1, POLDIP3 (SKAR) and UPF3B demonstrated by coimmunoprecipitation 738552 N/A 738562 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 738563 unspecified role unspecified role prey prey N/A 738557 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false Fig. 2A Q09161; Q9BZI7 transfection ncbp1-rps6-2 Interaction between NCBP1 and RPS6 identified by coimmunoprecipitation 738552 N/A 738557 unspecified role unspecified role bait bait sbp tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 738564 unspecified role unspecified role prey prey ser-240_ser244 (potential) opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 5A, Supp. Fig 2A, Supp. Fig. 2B A number of other proteins were shown to be bound to NCBP1 by anti-phosphoserine Abs but none were actually identified. siRNA studies showed POLDIP3 to be required for the insulin-stimulated increase in CBP80-bound phosphoproteins. Phosphorylation required RPS6KB1 but not RPS6KB2. Rapamycin inhibited insulin-induced phosphorylation of RPS6 100 mM insulin for 30 min 20 ng/mL rapamycin for 20 min + 100 mM insulin for 30 min eif3b-ncbp1-3 Interactors of NCBP1 identified by coimmunoprecipitation 738552 N/A 738564 unspecified role unspecified role prey prey N/A 738558 unspecified role unspecified role prey prey N/A 738559 unspecified role unspecified role prey prey N/A 738557 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false Fig. 5B 100 mM insulin for 30 mins 0 ng/ml rapamycin + 0 mM insulin RPS6 and RPS6KB1 showed insulin-dependent phosphorylation, siRNA studies suggest POLDIP3 is required for RPS6KB1 binding. eif3b-ncbp1-4 Interactors of NCBP1 identified by coimmunoprecipitation 738552 N/A 738559 unspecified role unspecified role prey prey N/A 738558 unspecified role unspecified role prey prey N/A 738557 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false Fig. 5B 0 mM insulin 20 ng/ml rapamycin for 20 mins + 100 mM insulin for 30 mins siRNA studies suggested binding of RPSkB! was POLDIP3-dependent. ncbp1-rps6-1 Interaction between NCBP1 and RPS6 identified by coimmunoprecipitation 738553 N/A 738557 unspecified role unspecified role bait bait N/A 738564 unspecified role unspecified role prey prey ser-240_ser244 (potential) opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1A A number of other proteins were shown to be bound to NCBP1 by anti-phosphoserine Abs but none were actually identified. 100 mM insulin for 30 min 20 ng/mL rapamycin for 20 min + 100 mM insulin for 30 min Rapamycin inhibited insulin-induced phosphorylation of RPS6 ncbp1-poldip3-1 Interaction between NCBP1 and POLDIP3 (SKAR) demonstrated by coimmunoprecipitation 738553 N/A 738557 unspecified role unspecified role prey prey N/A 738563 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2B eif4a3-poldip3-1 Interaction between EIF4A3, POLDIP3 (SKAR) and NCBP1 identified by coimmunopreciptiation 738553 N/A 738565 unspecified role unspecified role prey prey N/A 738563 unspecified role unspecified role bait bait N/A 738557 unspecified role unspecified role prey prey association association false false false Interaction with NCBP1 is RNA-dependent Fig. 4C rps6kb1-eif4e-6 Interaction between RPS6, EIF4E and RPS6KB1 by coimmunoprecipitation 738553 N/A 738558 unspecified role unspecified role prey prey N/A 738560 unspecified role unspecified role bait bait N/A 738564 unspecified role unspecified role prey prey association association false false false Fig. 5B 100 mM insulin for 30 mins 0 ng/ml rapamycin + 0 mM insulin RPS6 was Ser-240 and Ser-244 phosphorylated. ncbp1-eif3b-1 Interactors of NCBP1 identified by coimmunoprecipitation 738554 N/A 738558 unspecified role unspecified role prey prey N/A 738559 unspecified role unspecified role prey prey N/A 738557 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 738561 unspecified role unspecified role prey prey association association false false false Fig. 1B 100 20 ncbp1-eif3b-3 Interactors of NCBP1 identified by coimmunoprecipitation 738554 n/a 738558 unspecified role unspecified role prey prey n/a 738559 unspecified role unspecified role prey prey n/a 738557 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false Fig. 1B 0 0 ncbp1-eif3b-2 Interactors of NCBP1 identified by coimmunoprecipitation 738554 N/A 738559 unspecified role unspecified role prey prey N/A 738561 unspecified role unspecified role prey prey N/A 738557 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 738558 unspecified role unspecified role prey prey region nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 1B 100 0 Q09161 transfection RPS6KB1 is serine phosphorylated poldip3-sfrs2 Colocalisation of POLDIP3 with SFRS2 738555 N/A 738566 unspecified role unspecified role neutral component neutral component N/A 738563 unspecified role unspecified role neutral component neutral component n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Q9BY77 transfection Fig. 3A poldip3-sfrs2-1 Colocalisation of POLDIP3 with SFRS2 738555 N/A 738566 unspecified role unspecified role neutral component neutral component N/A 738563 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 3A poldip3-sfrs2-2 Colocalisation of POLDIP3 with SFRS2 738556 N/A 738563 unspecified role unspecified role neutral component neutral component N/A 738566 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 3A