IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ shinohara-2008-1 Tyrosine kinases Btk and Tec regulate osteoclast differentiation by linking RANK and ITAM signals. mouse-bmm mouse bone marrow derived macrophages bmm bone marrow derived macrophages Bone marrow derived macrophages (BMM) were generated in vitro from precursor cells harvested from the femurs of C57BL/6 mice (7�8 weeks) by proliferation and differentiation in the presence of L-cell conditioned medium. as a source of CSF-1 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Shinohara M., Koga T., Okamoto K., Sakaguchi S., Arai K., Yasuda H., Takai T., Kodama T., Morio T., Geha RS., Kitamura D., Kurosaki T., Ellmeier W., Takayanagi H. Apoptosis - Interactions involving proteins with a function related to apoptosis taka.csi@tmd.ac.jp Cell (0092-8674) 2008 Only protein-protein interactions 2 imex curation shinohara-2008-2 Tyrosine kinases Btk and Tec regulate osteoclast differentiation by linking RANK and ITAM signals. mouse-bmm mouse bone marrow derived macrophages bmm bone marrow derived macrophages Bone marrow derived macrophages (BMM) were generated in vitro from precursor cells harvested from the femurs of C57BL/6 mice (7�8 weeks) by proliferation and differentiation in the presence of L-cell conditioned medium. as a source of CSF-1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shinohara M., Koga T., Okamoto K., Sakaguchi S., Arai K., Yasuda H., Takai T., Kodama T., Morio T., Geha RS., Kitamura D., Kurosaki T., Ellmeier W., Takayanagi H. Apoptosis - Interactions involving proteins with a function related to apoptosis taka.csi@tmd.ac.jp Cell (0092-8674) 2008 Only protein-protein interactions 2 imex curation btk_mouse Tyrosine-protein kinase BTK Bruton tyrosine kinase B-cell progenitor kinase Kinase EMB Btk Bpk Agammaglobulinemia tyrosine kinase protein protein mouse Mus musculus Mouse MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVIPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPTAAPISTTELKKVVALYDYMPMNANDLQLRKGEEYFILEESNLPWWRARDKNGQEGYIPSNYITEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGEPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSKQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIREGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASERVYTIMYSCWHEKADERPSFKILLSNILDVMDEES blnk_mouse B-cell linker protein Cytoplasmic adapter protein B-cell adapter containing a SH2 domain protein B-cell adapter containing a Src homology 2 domain protein Src homology 2 domain-containing leukocyte protein of 65 kDa Lymphocyte antigen 57 Blnk Bash Ly57 Slp65 protein protein mouse Mus musculus Mouse MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMDKIKKLKVKGPPSVPRRDYALDSPADEEEQWSDDFDSDYENPDEHSDSEMYVMPAEETGDDSYEPPPAEQQTRVVHPALPFTRGEYVDNRSSQRHSPPFSKTLPSKPSWPSAKARLASTLPAPNSLQKPQVPPKPKDLLEDEADYVVPVEDNDENYIHPRESSPPPAEKAPMVNRSTKPNSSSKHMSPPGTVAGRNSGVWDSKSSLPAAPSPLPRAGKKPATPLKTTPVPPLPNASNVCEEKPVPAERHRGSSHRQDTVQSPVFPPTQKPVHQKPVPLPRFPEAGSPAADGPFHSFPFNSTFADQEAELLGKPWYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLVAFFNKRVYNIPVRFIEATKQYALGKKKNGEEYFGSVVEIVNSHQHNPLVLIDSQNNTKDSTRLKYAVKVS btk-blnk-1 Colocalisation between Btk and Blnk 729335 N/A 729337 unspecified role unspecified role neutral component neutral component N/A 729338 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Tnfsf11/Rankl Fig. 5A and Supp. Fig. 3 Colocalisation not observed in DAP12-/-FcRgamma-/- cells. btk-blnk-2 Interaction between Btk and Blnk determined by coimmunoprecipitation 729336 N/A 729338 unspecified role unspecified role bait bait N/A 729337 unspecified role unspecified role prey prey physical association physical association false false false Tnfsf11/Rankl Fig. 5B Interaction not seen in DAP12-/-FcRgamma-/- cells or Tec-/-Btk-/- cells.