IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ jelluma-2008-1 Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Jelluma N., Brenkman AB., van den Broek NJ., Cruijsen CW., van Osch MH., Lens SM., Medema RH., Kops GJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of TTK/MPS1 taken from PMID: 11927556 and mutation mapping. Source of Borealin identified from mapping of mass spec peptides. g.j.p.l.kops@umcutrecht.nl Cell (0092-8674) 2008 Only protein-protein interactions 3 imex curation jelluma-2008-2 Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference pull down pull down western blot western blot Immuno blot Immuno blot Jelluma N., Brenkman AB., van den Broek NJ., Cruijsen CW., van Osch MH., Lens SM., Medema RH., Kops GJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells treated with Nocodazole for 16 hr. Immunoprecipitates isolated following pulldown of LAP-Borealin using S-protein-Agarose. Source of TTK/MPS1 taken from PMID: 11927556 and mutation mapping. Source of Borealin identified from mapping of mass spec peptides. g.j.p.l.kops@umcutrecht.nl Cell (0092-8674) 2008 Only protein-protein interactions 3 imex curation ttk_human Dual specificity protein kinase TTK TTK MPS1L1 Phosphotyrosine picked threonine-protein kinase MPS1 protein protein human Homo sapiens Human MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGSLGHLQNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFGRVPVNLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSCELRNLKSVQNSHFKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETKEYQEPEVPESNQKQWQSKRKSECINQNPAASSNHWQIPELARKVNTEQKHTTFEQPVFSVSKQSPPISTSKWFDPKSICKTPSSNTLDDYMSCFRTPVVKNDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQVLASSSANECISVKGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLDSYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPWERKSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTYGKTPFQQIINQISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCLKRDPKQRISIPELLAHPYVQIQTHPVNQMAKGTTEEMKYVLGQLVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK borea_human Borealin Dasra-B Cell division cycle-associated protein 8 Pluripotent embryonic stem cell-related gene 3 protein CDCA8 PESCRG3 protein protein human Homo sapiens Human MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD aurkb_human Aurora kinase B AIRK2 Aurora- and IPL1-like midbody-associated protein 1 Aurora/IPL1-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 STK1 STK5 Aurora 1 AURKB AIK2 AIM1 ARK2 STK12 Serine/threonine-protein kinase aurora-B protein protein human Homo sapiens Human MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA cdca8-ttk-1 Phosphorylation of CDCA8 (Borealin ) by TTK (Mps1) 728908 N/A 728910 enzyme enzyme neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P33981:p.Asp664Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 728911 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation false false false Phosphorylation of CDCA8. Subsequent mass spec and mutagenesis studies show Thr-88, Thr-94, Thr-169 and Thr-230 of CDCA8 to be phosphorylated by TTK Figs. 6A, 6B Supp. Fig. 4 Asp664Ala is catalytically inactive ttk-1 Autophosphorylation of TTK (Mps1) 728908 N/A 728910 putative self putative self putative self putative self P33981:p.Asp664Ala mutation mutation certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation false true false Autophosphorylation of TTK (Mps1). Figs. 1B Autophosphorylation not present in kinase-dead asp664ala mutant. cdca8-aurkb-1 Interaction between CDCA8 (Borealin ), AURKB and BIRC5 (Survivin) identified by pulldown 728909 N/A 728911 unspecified role unspecified role bait bait lap tagged region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 728912 unspecified role unspecified role prey prey N/A 728913 unspecified role unspecified role prey prey association association false false false Figs. 6D Q53HL2 transfection