IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ frum-2007-1 HDM2-binding partners: interaction with translation elongation factor EF1alpha. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Frum R., Busby S.A., Ramamoorthy M., Deb S., Shabanowitz J., Hunt D.F., Deb S.P. Apoptosis - Interactions involving proteins with a function related to apoptosis HDM2 was overexpressed in H1299 cells. Extracts were subjected to immunoprecipitation with anti-HDM2 antibody directly coupled with agarose. Proteins eluted from HDM2 immunoprecipitations were digested with trypsin and analysed by nanospray HPLC mass spectrometry. spdeb@vcu.edu J. Proteome Res. (1535-3893) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer frum-2007-4 HDM2-binding partners: interaction with translation elongation factor EF1alpha. human-wi_38 Homo sapiens Caucasian fetal lung fibroblast cell line wi_38 WI39 human Caucasian fetal lung fibroblast cell line From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37C with periodic pH adjustments. Used in preparation of human virus vaccines. 2n = 46, diploid except at high passage number anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Frum R., Busby S.A., Ramamoorthy M., Deb S., Shabanowitz J., Hunt D.F., Deb S.P. Apoptosis - Interactions involving proteins with a function related to apoptosis HDM2 was overexpressed in H1299 cells. Extracts were subjected to immunoprecipitation with anti-HDM2 antibody directly coupled with agarose. Proteins eluted from HDM2 immunoprecipitations were digested with trypsin and analysed by nanospray HPLC mass spectrometry. spdeb@vcu.edu J. Proteome Res. (1535-3893) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer frum-2007-2 HDM2-binding partners: interaction with translation elongation factor EF1alpha. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Frum R., Busby S.A., Ramamoorthy M., Deb S., Shabanowitz J., Hunt D.F., Deb S.P. Apoptosis - Interactions involving proteins with a function related to apoptosis HDM2 was overexpressed in H1299 cells. Extracts were subjected to immunoprecipitation with anti-HDM2 antibody directly coupled with agarose. Proteins eluted from HDM2 immunoprecipitations were digested with trypsin and analysed by nanospray HPLC mass spectrometry. spdeb@vcu.edu J. Proteome Res. (1535-3893) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer frum-2007-3 HDM2-binding partners: interaction with translation elongation factor EF1alpha. human-sjsa_1 Homo sapiens SJSA-1 (formerly OsA-CL) osteosarcoma cells sjsa_1 SJSA-1 (formerly OsA-CL) osteosarcoma cells The SJSA-1 cell line (formerly OsA-CL) was established in 1982 from the primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur. There is amplification of the gene which encodes the p53 associated protein, MDM2. SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene. anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Frum R., Busby S.A., Ramamoorthy M., Deb S., Shabanowitz J., Hunt D.F., Deb S.P. Apoptosis - Interactions involving proteins with a function related to apoptosis HDM2 was overexpressed in H1299 cells. Extracts were subjected to immunoprecipitation with anti-HDM2 antibody directly coupled with agarose. Proteins eluted from HDM2 immunoprecipitations were digested with trypsin and analysed by nanospray HPLC mass spectrometry. spdeb@vcu.edu J. Proteome Res. (1535-3893) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer frum-2007-5 HDM2-binding partners: interaction with translation elongation factor EF1alpha. human-wi_38 Homo sapiens Caucasian fetal lung fibroblast cell line wi_38 WI39 human Caucasian fetal lung fibroblast cell line From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37C with periodic pH adjustments. Used in preparation of human virus vaccines. 2n = 46, diploid except at high passage number confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Frum R., Busby S.A., Ramamoorthy M., Deb S., Shabanowitz J., Hunt D.F., Deb S.P. Apoptosis - Interactions involving proteins with a function related to apoptosis HDM2 was overexpressed in H1299 cells. Extracts were subjected to immunoprecipitation with anti-HDM2 antibody directly coupled with agarose. Proteins eluted from HDM2 immunoprecipitations were digested with trypsin and analysed by nanospray HPLC mass spectrometry. spdeb@vcu.edu J. Proteome Res. (1535-3893) 2007 Only protein-protein interactions imex curation Cancer - Interactions investigated in the context of cancer tbb1_human Tubulin beta-1 chain TUBB1 protein protein human Homo sapiens Human MREIVHIQIGQCGNQIGAKFWEMIGEEHGIDLAGSDRGASALQLERISVYYNEAYGRKYVPRAVLVDLEPGTMDSIRSSKLGALFQPDSFVHGNSGAGNNWAKGHYTEGAELIENVLEVVRHESESCDCLQGFQIVHSLGGGTGSGMGTLLMNKIREEYPDRIMNSFSVMPSPKVSDTVVEPYNAVLSIHQLIENADACFCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGITTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTAQGSQQYRALSVAELTQQMFDARNTMAACDLRRGRYLTVACIFRGKMSTKEVDQQLLSVQTRNSSCFVEWIPNNVKVAVCDIPPRGLSMAATFIGNNTAIQEIFNRVSEHFSAMFKRKAFVHWYTSEGMDINEFGEAENNIHDLVSEYQQFQDAKAVLEEDEEVTEEAEMEPEDKGH rs20_human 40S ribosomal protein S20 RPS20 Small ribosomal subunit protein uS10 protein protein human Homo sapiens Human MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA dcd_human Dermcidin AIDD DSEP Preproteolysin DCD protein protein human Homo sapiens Human MRFMTLLFLTALAGALVCAYDPEAASAPGSGNPCHEASAAQKENAGEDPGLARQAPKPRKQRSSLLEKGLDGAKKAVGGLGKLGKDAVEDLESVGKGAVHDVKDVLDSVL if90l_human_protein Chain L, Fab Fragment Of Monoclonal Antibody (Lnkb-2) Against Human Interleukin-2 In Complex With Antigenic Peptide protein protein mouse Mus musculus Mouse DVQMTQTPLTLSVTIGQPASISCESSQSLLYSNGKTYLNWLLQRPGQSPKRLIYLVSKLDSGVPDRFTGSGSGTDFTLRISRVEAEDLGVYYCVQGTHFPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC An amino acid sequence which is not represented in UniProt tba1c_human Tubulin alpha-1C chain TUBA1C TUBA6 Tubulin alpha-6 chain Alpha-tubulin 6 protein protein human Homo sapiens Human MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGADSADGEDEGEEY rl11_human 60S ribosomal protein L11 RPL11 CLL-associated antigen KW-12 Large ribosomal subunit protein uL5 protein protein human Homo sapiens Human MAQDQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKYDGIILPGK cdn2b_human Cyclin-dependent kinase 4 inhibitor B CDKN2B MTS2 p14-INK4b p15-INK4b Multiple tumor suppressor 2 protein protein human Homo sapiens Human MREENKGMPSGGGSDEGLASAAARGLVEKVRQLLEAGADPNGVNRFGRRAIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEERGHRDVAGYLRTATGD rl5_human 60S ribosomal protein L5 RPL5 Large ribosomal subunit protein uL18 MSTP030 protein protein human Homo sapiens Human MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES g3pt_human Glyceraldehyde-3-phosphate dehydrogenase, testis-specific GAPDHS GAPD2 GAPDH2 GAPDS HSD35 HSD-35 Spermatogenic glyceraldehyde-3-phosphate dehydrogenase Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2 protein protein human Homo sapiens Human MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK ef1a1_human Elongation factor 1-alpha 1 EEF1A1 EEF1A EF1A LENG7 Eukaryotic elongation factor 1 A-1 Elongation factor Tu Leukocyte receptor cluster member 7 protein protein human Homo sapiens Human MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP k1c14_human Keratin, type I cytoskeletal 14 Cytokeratin-14 KRT14 Keratin-14 protein protein human Homo sapiens Human MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSSRFSSGGACGLGGGYGGGFSSSSSSFGSGFGGGYGGGLGAGLGGGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPAEIKDYSPYFKTIEDLRNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN mdm2-cdkn2b Mdm2 coimmunoprecipitates EF1alpha in H1299 cell extracts 606322 n/a 606327 unspecified role unspecified role prey prey n/a 606328 unspecified role unspecified role prey prey n/a 606329 unspecified role unspecified role prey prey n/a 606330 unspecified role unspecified role prey prey n/a 606331 unspecified role unspecified role prey prey n/a 606332 unspecified role unspecified role prey prey n/a 606333 unspecified role unspecified role prey prey n/a 606334 unspecified role unspecified role prey prey n/a 606335 unspecified role unspecified role prey prey N/A 606336 unspecified role unspecified role prey prey N/A 606337 unspecified role unspecified role bait bait association association false false false Table 1, 1 B Q00987 transfection Q00987 over-expressed BAIT protein gi266516; PREY protein gi4503571 The author identifier for the PREY protein does not correspond to eukaryotic elongation factor 1. mdm2-if90l_human_pro Mdm2 coimmunoprecipitates keratin 14 in H1299 cell extracts 606322 n/a 606330 unspecified role unspecified role prey prey N/A 606338 unspecified role unspecified role prey prey N/A 606337 unspecified role unspecified role bait bait association association false false false Table 1 Q00987 transfection Q00987 over-expressed BAIT protein gi266516; PREY protein gi1752236 mdm2-eef1a1-2 Mdm2 coimmunoprecipitates EF1alpha in WI38 cell lysates 606323 N/A 606336 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 606337 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3 A, B, C WI38 cells are not transformed. Q00987 transfection eef1a1-mdm2-1 Mdm2 coimmunoprecipitates with EF1alpha in WI38 cell lysates 606323 N/A 606337 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 606336 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3 B WI38 cells are not transformed. Q00987 transfection eef1a1-mdm2 Mdm2 coimmunoprecipitates with EF1alpha in H1299 cell extracts 606324 N/A 606336 unspecified role unspecified role bait bait N/A 606337 unspecified role unspecified role prey prey physical association physical association false false false 2 A Q00987 transfection BAIT protein gi266516; PREY protein gi4503571 The author identifier for the PREY protein does not correspond to eukaryotic elongation factor 1. mdm2-eef1a1-1 Mdm2 coimmunoprecipitates EF1alpha in SJSA-1 (OsACL) cells 606325 N/A 606336 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 606337 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2 B Q00987 over-expression eef1a1-mdm2-2 Mdm2 colocalizes with EF1alpha in the cytoplasm of WI38 cells 606326 N/A 606336 unspecified role unspecified role neutral component neutral component N/A 606337 unspecified role unspecified role neutral component neutral component physical association physical association false false false WI38 cells are not transformed. Q00987 transfection