UniProt UniProt http://www.uniprot.org hublitz-2005-2 NIR is a novel INHAT repressor that modulates the transcriptional activity of p53. in vitro In vitro pull down pull down autoradiography autoradiography Hublitz P., Kunowska N., Mayer U.P., Muller J.M., Heyne K., Yin N., Fritzsche C., Poli C., Miguet L., Schupp I.W., van Grunsven L.A., Potiers N., van Dorsselaer A., Metzger E., Roemer K., Schule R. Apoptosis - Interactions involving proteins with a function related to apoptosis The retained NIR activity prevents acetylation of all core histones by the p300 histone acetyltransferase Experiments from figure 3A: omitted because participants detection by coomasie staining is not specific. Experiment from figure 3B: peptide used is not specific to a known species (because protein sequences are identical between several species). Origin of p300 is human according to PMID: 7523245 Experiments from figure 3C: omitted because H3, H2B, H2A and H4 participants are used as a mixture and cannot be mapped to a UniProt AC number. Source of KAT2B inferred from sequence length of construct produced by contributing author. roland.schuele@uniklinik-freiburg.de Genes Dev. (0890-9369) 2005 mimix curation hublitz-2005-4 NIR is a novel INHAT repressor that modulates the transcriptional activity of p53. mesau-bhk Mesocricetus auratus baby kidney cell line BHK bhk Golden (Syrian) Hamster baby kidney cell line BHK Established from the kidneys of five unsexed, one-day-old hamsters in 1961. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hublitz P., Kunowska N., Mayer U.P., Muller J.M., Heyne K., Yin N., Fritzsche C., Poli C., Miguet L., Schupp I.W., van Grunsven L.A., Potiers N., van Dorsselaer A., Metzger E., Roemer K., Schule R. Apoptosis - Interactions involving proteins with a function related to apoptosis The retained NIR activity prevents acetylation of all core histones by the p300 histone acetyltransferase Experiments from figure 3A: omitted because participants detection by coomasie staining is not specific. Experiment from figure 3B: peptide used is not specific to a known species (because protein sequences are identical between several species). Origin of p300 is human according to PMID: 7523245 Experiments from figure 3C: omitted because H3, H2B, H2A and H4 participants are used as a mixture and cannot be mapped to a UniProt AC number. Source of KAT2B inferred from sequence length of construct produced by contributing author. roland.schuele@uniklinik-freiburg.de Genes Dev. (0890-9369) 2005 mimix curation hublitz-2005-9 NIR is a novel INHAT repressor that modulates the transcriptional activity of p53. in vitro In vitro acetylase assay acetylase assay autoradiography autoradiography Hublitz P., Kunowska N., Mayer U.P., Muller J.M., Heyne K., Yin N., Fritzsche C., Poli C., Miguet L., Schupp I.W., van Grunsven L.A., Potiers N., van Dorsselaer A., Metzger E., Roemer K., Schule R. Apoptosis - Interactions involving proteins with a function related to apoptosis The retained NIR activity prevents acetylation of all core histones by the p300 histone acetyltransferase Experiments from figure 3A: omitted because participants detection by coomasie staining is not specific. Experiment from figure 3B: peptide used is not specific to a known species (because protein sequences are identical between several species). Origin of p300 is human according to PMID: 7523245 Experiments from figure 3C: omitted because H3, H2B, H2A and H4 participants are used as a mixture and cannot be mapped to a UniProt AC number. Source of KAT2B inferred from sequence length of construct produced by contributing author. roland.schuele@uniklinik-freiburg.de Genes Dev. (0890-9369) 2005 mimix curation hublitz-2005-3 NIR is a novel INHAT repressor that modulates the transcriptional activity of p53. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. tap tandem affinity purification TAP TAP tap sequence tag sequence tag identification sequence tag Hublitz P., Kunowska N., Mayer U.P., Muller J.M., Heyne K., Yin N., Fritzsche C., Poli C., Miguet L., Schupp I.W., van Grunsven L.A., Potiers N., van Dorsselaer A., Metzger E., Roemer K., Schule R. Apoptosis - Interactions involving proteins with a function related to apoptosis The retained NIR activity prevents acetylation of all core histones by the p300 histone acetyltransferase Experiments from figure 3A: omitted because participants detection by coomasie staining is not specific. Experiment from figure 3B: peptide used is not specific to a known species (because protein sequences are identical between several species). Origin of p300 is human according to PMID: 7523245 Experiments from figure 3C: omitted because H3, H2B, H2A and H4 participants are used as a mixture and cannot be mapped to a UniProt AC number. Source of KAT2B inferred from sequence length of construct produced by contributing author. roland.schuele@uniklinik-freiburg.de Genes Dev. (0890-9369) 2005 mimix curation hublitz-2005-5 NIR is a novel INHAT repressor that modulates the transcriptional activity of p53. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hublitz P., Kunowska N., Mayer U.P., Muller J.M., Heyne K., Yin N., Fritzsche C., Poli C., Miguet L., Schupp I.W., van Grunsven L.A., Potiers N., van Dorsselaer A., Metzger E., Roemer K., Schule R. Apoptosis - Interactions involving proteins with a function related to apoptosis The retained NIR activity prevents acetylation of all core histones by the p300 histone acetyltransferase Experiments from figure 3A: omitted because participants detection by coomasie staining is not specific. Experiment from figure 3B: peptide used is not specific to a known species (because protein sequences are identical between several species). Origin of p300 is human according to PMID: 7523245 Experiments from figure 3C: omitted because H3, H2B, H2A and H4 participants are used as a mixture and cannot be mapped to a UniProt AC number. Source of KAT2B inferred from sequence length of construct produced by contributing author. roland.schuele@uniklinik-freiburg.de Genes Dev. (0890-9369) 2005 mimix curation hublitz-2005-6 NIR is a novel INHAT repressor that modulates the transcriptional activity of p53. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hublitz P., Kunowska N., Mayer U.P., Muller J.M., Heyne K., Yin N., Fritzsche C., Poli C., Miguet L., Schupp I.W., van Grunsven L.A., Potiers N., van Dorsselaer A., Metzger E., Roemer K., Schule R. Apoptosis - Interactions involving proteins with a function related to apoptosis The retained NIR activity prevents acetylation of all core histones by the p300 histone acetyltransferase Experiments from figure 3A: omitted because participants detection by coomasie staining is not specific. Experiment from figure 3B: peptide used is not specific to a known species (because protein sequences are identical between several species). Origin of p300 is human according to PMID: 7523245 Experiments from figure 3C: omitted because H3, H2B, H2A and H4 participants are used as a mixture and cannot be mapped to a UniProt AC number. Source of KAT2B inferred from sequence length of construct produced by contributing author. roland.schuele@uniklinik-freiburg.de Genes Dev. (0890-9369) 2005 mimix curation hublitz-2005-7 NIR is a novel INHAT repressor that modulates the transcriptional activity of p53. human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hublitz P., Kunowska N., Mayer U.P., Muller J.M., Heyne K., Yin N., Fritzsche C., Poli C., Miguet L., Schupp I.W., van Grunsven L.A., Potiers N., van Dorsselaer A., Metzger E., Roemer K., Schule R. Apoptosis - Interactions involving proteins with a function related to apoptosis The retained NIR activity prevents acetylation of all core histones by the p300 histone acetyltransferase Experiments from figure 3A: omitted because participants detection by coomasie staining is not specific. Experiment from figure 3B: peptide used is not specific to a known species (because protein sequences are identical between several species). Origin of p300 is human according to PMID: 7523245 Experiments from figure 3C: omitted because H3, H2B, H2A and H4 participants are used as a mixture and cannot be mapped to a UniProt AC number. Source of KAT2B inferred from sequence length of construct produced by contributing author. roland.schuele@uniklinik-freiburg.de Genes Dev. (0890-9369) 2005 mimix curation hublitz-2005-10 NIR is a novel INHAT repressor that modulates the transcriptional activity of p53. mesau-bhk Mesocricetus auratus baby kidney cell line BHK bhk Golden (Syrian) Hamster baby kidney cell line BHK Established from the kidneys of five unsexed, one-day-old hamsters in 1961. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hublitz P., Kunowska N., Mayer U.P., Muller J.M., Heyne K., Yin N., Fritzsche C., Poli C., Miguet L., Schupp I.W., van Grunsven L.A., Potiers N., van Dorsselaer A., Metzger E., Roemer K., Schule R. Apoptosis - Interactions involving proteins with a function related to apoptosis The retained NIR activity prevents acetylation of all core histones by the p300 histone acetyltransferase Experiments from figure 3A: omitted because participants detection by coomasie staining is not specific. Experiment from figure 3B: peptide used is not specific to a known species (because protein sequences are identical between several species). Origin of p300 is human according to PMID: 7523245 Experiments from figure 3C: omitted because H3, H2B, H2A and H4 participants are used as a mixture and cannot be mapped to a UniProt AC number. Source of KAT2B inferred from sequence length of construct produced by contributing author. roland.schuele@uniklinik-freiburg.de Genes Dev. (0890-9369) 2005 mimix curation noc2l_human Nucleolar complex protein 2 homolog NOC2-like protein NOC2L NIR Novel INHAT repressor protein protein human Homo sapiens Human MAAAGSRKRRLAELTVDEFLASGFDSESESESENSPQAETREAREAARSPDKPGGSPSASRRKGRASEHKDQLSRLKDRDPEFYKFLQENDQSLLNFSDSDSSEEEEGPFHSLPDVLEEASEEEDGAEEGEDGDRVPRGLKGKKNSVPVTVAMVERWKQAAKQRLTPKLFHEVVQAFRAAVATTRGDQESAEANKFQVTDSAAFNALVTFCIRDLIGCLQKLLFGKVAKDSSRMLQPSSSPLWGKLRVDIKAYLGSAIQLVSCLSETTVLAAVLRHISVLVPCFLTFPKQCRMLLKRMVIVWSTGEESLRVLAFLVLSRVCRHKKDTFLGPVLKQMYITYVRNCKFTSPGALPFISFMQWTLTELLALEPGVAYQHAFLYIRQLAIHLRNAMTTRKKETYQSVYNWQYVHCLFLWCRVLSTAGPSEALQPLVYPLAQVIIGCIKLIPTARFYPLRMHCIRALTLLSGSSGAFIPVLPFILEMFQQVDFNRKPGRMSSKPINFSVILKLSNVNLQEKAYRDGLVEQLYDLTLEYLHSQAHCIGFPELVLPVVLQLKSFLRECKVANYCRQVQQLLGKVQENSAYICSRRQRVSFGVSEQQAVEAWEKLTREEGTPLTLYYSHWRKLRDREIQLEISGKERLEDLNFPEIKRRKMADRKDEDRKQFKDLFDLNSSEEDDTEGFSERGILRPLSTRHGVEDDEEDEEEGEEDSSNSEDGDPDAEAGLAPGELQQLAQGPEDELEDLQLSEDD h31_human Histone H3.1 H3FA H3FL H3FD H3FI H3FH H3FK H3FF H3FJ H3FB Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l H3C1 HIST1H3A H3C2 HIST1H3B H3C3 H3FC HIST1H3C H3C4 HIST1H3D H3C6 HIST1H3E H3C7 HIST1H3F H3C8 HIST1H3G H3C10 HIST1H3H H3C11 HIST1H3I H3C12 HIST1H3J protein protein human Homo sapiens Human MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA h32_human Histone H3.2 H3C13 H3C14 H3C15 H3-clustered histone 13 H3-clustered histone 14 H3-clustered histone 15 H3F2 H3FM HIST2H3A HIST2H3C HIST2H3D Histone H3/m Histone H3/o protein protein human Homo sapiens Human MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA h33_human Histone H3.3 H3-3A H3-3B H3.3A H3.3B H3F3 H3F3A H3F3B PP781 protein protein human Homo sapiens Human MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD kat2b_human Histone acetyltransferase KAT2B P300/CBP-associated factor PCAF Lysine acetyltransferase 2B Histone acetyltransferase PCAF KAT2B Spermidine acetyltransferase KAT2B protein protein human Homo sapiens Human MSEAGGAGPGGCGAGAGAGAGPGALPPQPAALPPAPPQGSPCAAAAGGSGACGPATAVAAAGTAEGPGGGGSARIAVKKAQLRSAPRAKKLEKLGVYSACKAEESCKCNGWKNPNPSPTPPRADLQQIIVSLTESCRSCSHALAAHVSHLENVSEEEMNRLLGIVLDVEYLFTCVHKEEDADTKQVYFYLFKLLRKSILQRGKPVVEGSLEKKPPFEKPSIEQGVNNFVQYKFSHLPAKERQTIVELAKMFLNRINYWHLEAPSQRRLRSPNDDISGYKENYTRWLCYCNVPQFCDSLPRYETTQVFGRTLLRSVFTVMRRQLLEQARQEKDKLPLEKRTLILTHFPKFLSMLEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINPPPVAGTISYNSTSSSLEQPNAGSSSPACKASSGLEANPGEKRKMTDSHVLEEAKKPRVMGDIPMELINEVMSTITDPAAMLGPETNFLSAHSARDEAARLEERRGVIEFHVVGNSLNQKPNKKILMWLVGLQNVFSHQLPRMPKEYITRLVFDPKHKTLALIKDGRVIGGICFRMFPSQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHDILNFLTYADEYAIGYFKKQGFSKEIKIPKTKYVGYIKDYEGATLMGCELNPRIPYTEFSVIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKDGVRQIPIESIPGIRETGWKPSGKEKSKEPRDPDQLYSTLKSILQQVKSHQSAWPFMEPVKRTEAPGYYEVIRFPMDLKTMSERLKNRYYVSKKLFMADLQRVFTNCKEYNPPESEYYKCANILEKFFFSKIKEAGLIDK h4_drome Histone H4 H4 His4r H4r CG3379 His4:CG31611 CG31611 His4 His4:CG33869 His4:CG33871 His4:CG33873 His4:CG33875 His4:CG33877 His4:CG33879 His4:CG33881 His4:CG33883 His4:CG33885 His4:CG33887 His4:CG33889 His4:CG33891 His4:CG33893 His4:CG33895 His4:CG33897 His4:CG33899 His4:CG33901 His4:CG33903 His4:CG33905 His4:CG33907 His4:CG33909 CG33869 CG33871 CG33873 CG33875 CG33877 CG33879 CG33881 CG33883 CG33885 CG33887 CG33889 CG33891 CG33893 CG33895 CG33897 CG33899 CG33901 CG33903 CG33905 CG33907 CG33909 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG h3_drome Histone H3 His3 His3:CG31613 His3:CG33857 His3:CG33860 His3:CG33863 His3:CG33866 CG31613 CG33803 CG33806 CG33809 CG33812 CG33815 CG33818 CG33821 CG33824 CG33827 CG33830 CG33833 CG33836 CG33839 CG33842 CG33845 CG33848 CG33851 CG33854 CG33857 CG33860 CG33863 CG33866 His3:CG33803 His3:CG33806 His3:CG33809 His3:CG33812 His3:CG33815 His3:CG33818 His3:CG33821 His3:CG33824 His3:CG33827 His3:CG33830 His3:CG33833 His3:CG33836 His3:CG33839 His3:CG33842 His3:CG33845 His3:CG33848 His3:CG33851 His3:CG33854 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA hist1h3a-noc2l 1234521 n/a 1234529 unspecified role unspecified role prey prey n/a 1234530 unspecified role unspecified role bait bait physical association physical association false false false 3B hist2h3a-noc2l 1234521 n/a 1234529 unspecified role unspecified role prey prey n/a 1234531 unspecified role unspecified role bait bait physical association physical association false false false 3B h3f3a-noc2l 1234521 n/a 1234529 unspecified role unspecified role prey prey n/a 1234532 unspecified role unspecified role bait bait physical association physical association false false false 3B tp53-noc2l 1234521 n/a 1234533 unspecified role unspecified role bait bait n/a 1234529 unspecified role unspecified role prey prey direct interaction direct interaction false false false 5B tp53-noc2l-1 1234522 n/a 1234533 unspecified role unspecified role bait bait n/a 1234529 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Fig 11B noc2l-tp53-2 1234522 n/a 1234529 unspecified role unspecified role bait bait n/a 1234533 unspecified role unspecified role prey prey physical association physical association false false false 5C his4r-kat2b 1234523 n/a 1234534 enzyme enzyme neutral component neutral component n/a 1234535 enzyme target enzyme target substrate substrate neutral component neutral component physical association physical association false false false supplementary fig.5 (lower panel) his3-his4r 1234523 n/a 1234534 enzyme enzyme neutral component neutral component n/a 1234536 enzyme target enzyme target substrate substrate neutral component neutral component physical association physical association false false false supplementary fig.5 (lower panel) noc2l-tp53-1 1234524 n/a 1234529 unspecified role unspecified role bait bait n/a 1234533 unspecified role unspecified role prey prey association association false false false Supplementary fig 9 noc2l-tp53-4 1234525 n/a 1234533 unspecified role unspecified role prey prey n/a 1234529 unspecified role unspecified role bait bait physical association physical association false false false 5D (Upper panel) noc2l-tp53-5 1234526 n/a 1234529 unspecified role unspecified role bait bait n/a 1234533 unspecified role unspecified role prey prey physical association physical association false false false 5D (lower panel) noc2l-tp53-6 1234527 n/a 1234529 unspecified role unspecified role bait bait n/a 1234533 unspecified role unspecified role prey prey physical association physical association false false false Supplementary Fig. 11A tp53-noc2l-2 1234528 n/a 1234529 unspecified role unspecified role prey prey n/a 1234533 unspecified role unspecified role bait bait physical association physical association false false false Supplementary Fig 11B